KWP-100 Operation Manual

User Manual

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 Table of Contents

1. Overview .......................................................................................... Error! Bookmark not defined.

1.1 System structure .................................................................................................................... 3

1.2 Basic Principles ..................................................................................................................... 4

2. Specifications and Parameters ..................................................... Error! Bookmark not defined.

3. Installation ....................................................................................... Error! Bookmark not defined.

3.1 Unpacking .............................................................................................................................. 7

3.2 Standard Configuration .......................................................................................................... 7
3.3 Environmental Requirements ................................................................................................ 7
3.4 Connection ............................................................................................................................. 7

4. Operation ......................................................................................... Error! Bookmark not defined.

4.1 Power-on.............................................................................................................................. 10
4.2 Operation Procedures ......................................................................................................... 10
4.3 Shutdown ............................................................................................................................. 12
4.4 Precautions on Use ............................................................................................................. 13

5. Maintenance and Storage .............................................................. Error! Bookmark not defined.

5.1 Cleaning ............................................................................................................................... 14

5.2 Maintaining and replacing the duct ...................................................................................... 14
5.3 Replacing the wash head .................................................................................................... 14
5.4 Unclogging the wash head .................................................................................................. 14
5.5 Replacing the fuse ............................................................................................................... 14
5.6 Storage................................................................................................................................. 14

6. Troubleshooting ............................................................................. Error! Bookmark not defined.

7. Warning............................................................................................ Error! Bookmark not defined.

Erba LISAWASH User Manual 2

 1. Overview

Erba Lisawash Elisa Washer

1.1 System structure

The appearance of the Erba Lisawash Elisa Washer is as shown in figure 1:

(5)
(6)

(2)

(1)

(3)

(4)

Figure 1

(1) Main unit (2) Touch Screen (3) Wash Head

(4) Plate Tray (5) Detergent Bottle (6) Waste Bottle

The system consists of pumps, controlling mechanism, wash head, plate tray, circuit, and bottles.

The pumps include a water pump for transporting detergent to wash head and a vacuum pump for

evacuating the waste from the ELISA plate. The controlling mechanism is primarily used to control

Erba LISAWASH User Manual 3

the up/down and left/right movement of wash head. Wash head is available in 8 or 12 way manifold

for 8×12 and 12×8 plates respectively.

The circuit is an advanced single chip system composed of high speed CPU.

The Erba Lisawash Elisa Washer is equipped with a touch screen, which makes operation

extremely convenient and easy.

The system includes a distilled water bottle, two wash bottles, and a waste bottle with overflow

sensors.

1.2 Basic Principles

Under control of the valve, the detergent is transported by the water pump to the ELISA plate on the

plate tray, through the wash head. The wash head moves up/down and left/right to add the detergent

into the plate wells. After a certain time of soaking, the vacuum pump sucks the Microwell contents

to the waste bottle. The ELISA plate can be washed efficiently through repetition of this process.

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 2. Specifications and Parameters

User Interface : Touch Screen LCD, 128x64 pixels with backlight

Wash head : 8 and 12 head manifold compatible

Wash mode : Row and plate

Wash rows : 1-12 rows

Plate Type : 96 well plate ( V, U and Flat)

No. of Plate presets : 10 Nos.

Wash protocols : 64 Nos.

Moving Cycles : 1-9 cycles

Aspiration Cycles : 1-9 cycles

Dispensing Volume : 50-450µl in 5µl increments

Dispensing Precision : <5% at 300µl

Aspiration Pressure : Programmable 2 Types

Soak Time : 255s

Residual Volume : <=2 µl for V/U bottom plates, and <=3 µl for Flat

Nos. of Bottles : 2 Wash, 1 D/W, 1 Waste

Bottle capacity : >2L Wash, D/W; Waste > 3L

Prime volume : 50-700µl in steps of 5µl increments

Clear bottom : Yes/No

Wash bottom : Yes/No

Keypad : Touch screen

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Operating Environment

Input power : <80 W

Power supply : AC220V/110V, 50/60Hz

Ambient temperature : 10ºC to 40ºC

Storage temperature : 10ºC to 50ºC

Relative humidity : Upto 90% relative humidity without condensation

Weight : 7.5Kg (Bottles not included)

Dimension : 380mm (H) ×350mm (W) ×190mm (D)

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 3. Installation

3.1 Unpacking

Upon opening the package, check the main unit and accessories against the packing list. If you find

anything damaged or missing, please contact your supplier immediately.

3.2 Standard Configuration

Name Qty. Name Qty.

Main unit 1 Bottle (2L) 3

Power cord 1 Waste bottle (3.5L) 1

8-head wash head 1 Plate tray 1

12-head wash head 1 Ground wire 1

Ø3.5 silicone tube 1 Fuse (2A) 2

Needle 1 Operation Manual 1

3.3 Environmental Requirements

The environment should be free of dust, corrosive gases, strong direct electromagnetic interference

and sunlight. The working platform should be firm and reliable, and the room should have good

ventilation.

3.4 Connection

3.4.1 Connect the air pump tube (blue) on the waste bottle to the Air Pump port (blue) on the back of

the device, as shown in figure 2.

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 ((1)
1) ((2)
2) ((3) 4)(5)
3) ((4)( 5)((6)
6) ((7)
7)

((8)
8) 10) ((11)
9) ((10)
((9) 11)

Figure 2

(1) Power switch (2) Air Pump port (3) Vent port

(4) Waste port (5) Detergent port (6) Duct hole

(7) Valve (8) Grounding (9) Water Pump

(10) Overflow Sensor plug (11) Power socket

3.4.2 Connect the waste tube (green) on the waste bottle to the Waste port (green) on the back of

the device.

3.4.3 Connect the tube (yellow) on the wash bottle to the Wash port (yellow) on the back of the

device.

3.4.4 Put the duct next to the valve into the round hole of the valve by pressing the valve core on the

top of the valve.

NOTE: The duct must be led to the round hole.

3.4.5 Insert the overflow sensor wire (gray) on the waste bottle into the Overflow Sensor plug on the

back of the system.

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3.4.6 Insert the two-wire cord (water pump supply lead) on the detergent bottle into the Water Pump

port on the back of the system.

3.4.7 Connect a proper ground wire to the Grounding on the back of the system.

3.4.8 Set the Power switch to off (O). Then insert the power cord into the Power socket on the back

of the device and switch on the power.

3.4.9 Unscrew the wash bottle cap (hold the cap with your left hand and rotate the bottle clockwise

with your right hand) and add sufficient detergent, then tighten the cap.

3.4.10 Add sufficient distilled water into the water bottle.

3.4.11 Tighten the waste bottle cap.

NOTE: When fixing the bottle cap, check if the white O-ring in the cap is damaged or placed

correctly.

3.4.12 Position the plate tray on the four poles, in the front base of the device, and push it into its

place.

NOTE: The Priming trough should be on the left.

3.4.13 Fix the 8-head (or 12-head) wash head on the wash arm. Sleeve the thinner tube of the wash

arm on the inlet port of the wash head and the thicker one on the outlet port.

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 4. Operation

4.1 Power-on

Switch on the system, it starts initialization and then the Main Menu is displayed on the screen.

Main Menu

Plate Settings Program Settings

System Settings Wash Program

4.2 Operation Procedures

4.2.1 System Settings

This function is used to set the number of wash heads, start position of wash head and pump speed.

4.2.1.1 Wash Head Settings

You should select corresponding wash head type according to the actual device configuration.

Method: Touch System Settings ( indicates being selected) and Wash Head

Settings in the submenu, then select “8” or “12” accordingly.

4.2.1.2 Start Pos. Settings

This function is used to adjust the wash head from the start position to the center of the wells of the

first row (Note: Since the start position of the 8- and 12-head wash heads is different, it should be

reset after replacing the wash head). Method: Touch Start Pos. Settings and select the wash

head type, then touch Increment or Decrease to set a value (8-head 330 by default;

12-head 490 by default). Touch OK and go to “Demo” menu. Touch Yes . Check whether

the wash head stops at the center of the first row. Increase or decrease the value until the required

setting is reached. Then check it by the demo program again. After the adjustment, touch OK

and No to return.

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4.2.1.3 Pump Speed

There are two flow speeds available for the pumps (you can control the prime volume and prime

pressure through the valve). Method: Touch Pump Speed and select 1 or 2 (2 is faster than 1),

then touch OK to confirm and return.

4.2.2 Plate Settings

This function is used to set the V, U, or Flat bottom plate. Different plates are stored in different No.

(1-8), which is easy to refer when editing wash programs.

Method: Touch Plate Settings and set a plate Number in the submenu. In the Prime Height

screen, select a height (about 70), then touch Demo to check whether the longer probes on

the wash head reach the desired height. Increase or decrease the value till desired setting is

reached. After confirming with the demo program, touch No to proceed to Aspirate Height.

Select a height about 150 (you’d better increase from Low to High to avoid damaging the wash

head), then touch Demo to check if the longer probes on the wash head reach the well bottom

with a spacing of about 0.3-0.5mm. If not enough, increase; otherwise decrease. Then touch No

to proceed to Crosswise aspirate . The aspiration of the fluid in the well bottom is done by

moving the probes back and forth. This function only applies to the flat bottom plates. Suppose plate

No.1 is a flat bottom plate, you can select Yes and then proceed to Range, which is used to set

the range setting to clean the well bottom. Set a value within 2-40 (about 30), then touch Demo

to check and make sure the probes will not touch the wells. When confirmed all the relevant data will

be saved in No.1 plate. Return to the main menu and proceed to settings of No.2…8 plates.

4.2.3 Program Settings

Based on ELISA assay requirements, we must have different wash modes and processes, namely

wash program. Program Settings is where you can edit various wash modes and save them for

future use.

Method: Touch Program Settings and select a program no. (1-40).

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Select a plate Number to select a Microwell plate type. Proceed to Moving Cycle (1-9). Set a value

and touch OK to proceed to Row Cycle. To wash for 5 times, if you select “Yes” for Row Cycle,

then it will wash every row for 5 times and go to the next row; if you select “No” for Row Cycle, then it

will wash from the first row to the 12th (8th) row and then repeat for another 4 times.

Select Yes or No . Proceed to Aspirate Cycle (1-9), set a value, and confirm.

Go to Prime Vol. (50µl - 700µl), which is commonly set to 300µl. Set a value and confirm.

In Prime Bottom setting, select Yes or No . Proceed to Prime Bottom Vol. (50µl - 700µl).

Set a value and confirm and go to Prime Bottom Cycle (1-9), which refers to number of the well

bottom washing. Set a value and confirm. For the Soak Time (0-255s), set a time as per the assay

protocol and confirm. Then this wash program will be saved. Return to the main menu and proceed

to settings of the next program.

4.2.4 Wash Program

You can start washing the plate when you finish the settings for the system and plate program.

Method: Touch the Wash Program button and select a program No., you can select the

program you want from the programs set. Confirm and proceed to Rows No. (1-12). For 8×12 plates,

the maximum is 12; for the 12×8 plates, the maximum is 8. Set a value and confirm, then the system

will start washing the plate. During washing process, touch Pause to pause. Then select

Stop to quit or Resume , for continue. If the waste bottle is full during washing, the screen

will display “Waste Overflow” and the washing will stop.

4.3 Shutdown

When the system is not used, the buffer salt in the detergent may be solidified, thus blocking the

wash head. To avoid this situation, perform the distilled water prime.

4.4.1 Daily Shutdown

Before shutdown of the system, touch the Wash Program button and run the distilled water

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prime. After washing, switch off the power supply and empty the waste bottle, then fill the Priming

trough on the plate tray with distilled water and soak the wash head.

4.4.2 Shutdown for Long Time

Wash the whole fluid system with distilled water prime for several times. Take out the duct from the

valve. Empty the detergent bottle and waste bottle, and then clean them with distilled water.

4.4 Precautions:

4.4.1 Empty the waste bottle regularly.

Dispose of the waste in accordance with your hospital guidelines for biohazard waste

disposal.

4.4.2 Before starting the ELISA wash, check if the No. of heads of the installed wash head is in

accordance with the settings. If you only want to wash part of the plate, please select corresponding

No. of rows.

4.4.3 The selected type of plate should be consistent with the one you are using.

Note that the programs with Crosswise aspirate function can’t be applied to the V, U

bottom plates, for it may damage the wash head.

4.4.4 It is strongly recommended to run wash program #41 for 2 times after the daily work.

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 5. Maintenance and Storage

5.1 Cleaning

The detergents used can be corrosive, so regular cleaning becomes very necessary. Please take
out the plate tray, clean the device with a soft cloth with neutral detergent and dry it. Clean the cover
of the device regularly.

5.2 Maintaining and replacing the duct

The duct is probably unable to restore as a result of long closing of the control valve. However, you
can press the valve core on the top of the valve and pull out one end of the duct a little.
Replace the duct after being used for a long time.

5.3 Replacing the wash head

Refer to 3.4.13.

5.4 Unclogging the wash head

Remove the wash head and then unclog the probes of the wash head with the spare needle.

5.5 Replacing the fuse

The fuse is located in the lower part of the power socket on the back of the device. Open the fuse
holder with a screwdriver and then replace the fuse with the spare 2A fuse.

5.6 Storage

The device should be stored in a room with good ventilation and relative humidity no greater than
80% and without corrosive gas.

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 6. Troubleshooting

Symptoms Causes Actions to be taken

1. The 220V power supply is Restore the normal power supply

No response when
powering on the device
The fuse is broken again
after being replaced
No detergent is dispensed
abnormal
Replace the fuse
2. The fuse is broken
1. Power supply is greater than Restore the normal power supply
242V
2. The device goes wrong Contact local distributor
1. The plug of the water pump is Insert it again properly
not inserted in place
2. The position of the water
pump is not good, it blocks the Change the position of the water
duct pump
3. The duct cannot be restored
4. The duct is broken Refer to 5.2

Replace the duct

1. The tube is leaking
2. The waste bottle is leaking
Can’t aspirate
3. The probe on wash head is
blocked
The tube is broken or connection is
loose
Check if the bottle cap is broken or
tightened
Unclog the probe with the needle

1. The moving part is blocked Clean the moving part and remove
The wash head moves
2. The system goes wrong the barrier
up/down or left/right
Contact local distributor
abnormally

1. The plug of the overflow sensor Insert the plug again properly
“Waste Overflow” is is loose
displayed when the waste 2. The overflow sensor is Replace the overflow sensor
bottle is not full defective

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Liquid drops at duct tube or
aspirate tube
High residual volume
Erba LISAWASH User Manual
1. The connections from duct tube
or aspirate tube to wash head is
loose or leaking
2. The tubing system is leaking
3. The duct tube is distortion
1. The ELISA plate is not placed
well
2. The Aspirate Height value is
not correct
3. The Start Pos. Setting is not
good
Tighten the tubes or change it if
necessary
Check the tubing system
Replace the duct tube
Place the plate again
Change the Aspirate Height value
and make sure the probes can
reach the well bottom
Change the Start Pos. Setting and
make sure the probe can reach the
center of the wells
16
 7. Warning

Erba Lisawash is a compact in vitro diagnosis instrument. It may bring risks to the patients

indirectly, and accompanying risks to the operator and environment. To avoid these risks, be

sure to read the following instructions carefully.

7.1. This device adopts AC 220V/110V which may bring electrical harm to human body.

Don’t open the rear cover before switching off the power supply. If you need to open the rear

cover to repair the device, contact our service personnel or professionals.

7.2. Since the patient samples may contain germs or virus which will cause disease,

be sure to dispose of the waste in accordance with relevant regulations of your hospital.

Transport and store the patient samples and handle the sample tubes strictly as instructed in

relevant regulations of your hospital.

7.3. Don’t operate the device unless you have read this Operation Manual carefully.

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