Power-on:

Switch on the system, it starts initialization and then the Main Menu is displayed on the screen.
Main Menu
Plate Settings Program Settings
System Settings Wash Program

Operation Procedures

System Settings
This function is used to set the type of Wash head, start position of wash head and pump speed.

Wash Head Settings

Select corresponding wash head type according to the actual device configuration.

Method: Touch System Settings (indicates being selected) and Wash Head Settings in the
submenu, then select “8” or “12” accordingly.

Start Pos. Settings

This function is used to adjust the wash head from the start position to the centre of the wells of
the first row. (Note: Since the start position of the 8- and 12-head wash heads is different, it should be
reset after replacing the wash head).

Method: Touch Start Pos. Settings and select the wash head type, then touch Increase or
Decrease to set a value (8-head 330 by default; 12-head 490 by default).

Touch OK and go to “Demo” menu.

Touch Yes. Check whether the wash head stops at the centre of the first row.

Increase or decrease the value until the required setting is reached.

Then check it by the demo program again. After the adjustment, touch OK and No to return.

Pump Speed
There are two flow speeds available for the pumps (you can control the prime volume and prime
pressure through the valve).

Method: Touch Pump Speed and select 1 or 2 (2 is faster than 1), then touch OK to confirm
and return.

Plate Settings
This function is used to set the V, U, or Flat bottom plate.
Different plates are stored in different No. (1-8), which is easy to refer when editing wash
programs.
Method: Touch Plate Settings and set a plate Number in the submenu.

In the Prime Height screen, select a height (about 70), then touch Demo to check whether the
longer probes on the wash head reach the desired height.
Increase or decrease the value till desired setting is reached.

After confirming with the demo program, touch No to proceed to Aspirate Height.

Select a height about 150 (you’d better increase from Low to High to avoid damaging the wash
head), then touch Demo to check if the longer probes on the wash head reach the well bottom
with a spacing of about 0.3-0.5mm.

If not enough, increase; otherwise decrease.

Then touch No to proceed to Crosswise aspirate.

The aspiration of the fluid in the well bottom is done by moving the probes back and forth.

This function only applies to the flat bottom plates. Suppose plate No.1 is a flat bottom plate,
you can select Yes and then proceed to Range, which is used to set the range setting to clean
the well bottom.

Set a value within 2-40 (about 30), then touch Demo to check and make sure the probes will not
touch the wells. When confirmed all the relevant data will be saved in No.1 plate.

Return to the main menu and proceed to settings of No.2…8 plates.

Program Settings

Based on ELISA assay requirements, we must have different wash modes and processes,
namely wash program. Program Settings is where you can edit various wash modes and save
them for future use.

Method: Touch Program Settings and select a program no. (1-40).

Select a plate Number to select a Microwell plate type. Proceed to Moving Cycle (1-9). Set
a value and touch OK to proceed to Row Cycle.

To wash for 5 times, if you select “Yes” for Row Cycle, then it will wash every row for 5
times and go to the next row; if you select “No” for Row Cycle, then it will wash from the
first row to the 12th (8th) row and then repeat for another 4 times.

Select Yes or No. Proceed to Aspirate Cycle (1-9), set a value, and confirm.

Go to Prime Vol. (50μl - 700μl), which is commonly set to 300μl. Set a value and confirm.

In Prime Bottom setting, select Yes or No. Proceed to Prime Bottom Vol. (50μl - 700μl).

Set a value and confirm and go to Prime Bottom Cycle (1-9), which refers to number of
the well bottom washing.

Set a value and confirm. For the Soak Time (0-255s), set a time as per the assay protocol
and confirm. Then this wash program will be saved. Return to the main menu and proceed
to settings of the next program.
Wash Program
You can start washing the plate when you finish the settings for the system and plate
program.

Method: Touch the Wash Program button and select a program No., you can select the
program you want from the programs set. Confirm and proceed to Rows No. (1-12).
For 8×12 plates, the maximum is 12; for the 12×8 plates, the maximum is 8. Set a value
and confirm, then the system will start washing the plate.

During washing process, touch Pause to pause.

Then select Stop to quit or Resume for continuing the Wash.
If the waste bottle is full during washing, the screen will display “Waste Overflow” and the
washing will stop.

Shutdown

When the system is not used, the buffer salt in the detergent may be solidified, thus
blocking the wash head. To avoid this situation, perform the distilled water prime.

Daily Shutdown

Before shutdown of the system, touch the Wash Program button and run the distilled
water prime.

After washing, switch off the power supply and empty the waste bottle, then fill the Priming
trough on the plate tray with distilled water and soak the wash head.

Shutdown for Long Time

Wash the whole fluid system with distilled water prime for several times.
Take out the duct from the valve.
Empty the detergent bottle and waste bottle, and then clean them with distilled water.

Precautions:

Empty the waste bottle regularly.

Dispose of the waste in accordance with your hospital guidelines for biohazard waste
disposal.

Before starting the ELISA wash, check if the No. of heads of the installed wash head is in
accordance with the settings.
If you only want to wash part of the plate, please select corresponding No. of rows.

The selected type of plate should be consistent with the one you are using.

Note that the programs with Crosswise aspirate function can’t be applied to the V, U bottom
plates, for it may damage the wash head.