Standard Manuscript Template

Remove all gray text before submitting

1This template contains the sections and formatting for a submission to JoVE.
2Begin writing directly in this document and remove the header, the footer, links, and any gray
3text prior to submission. The submitted manuscript should include original text, not previously
4published online or in print, including journals, websites or blogs.
5
6TITLE: (Instructions)
7Enter text here.
8
9AUTHORS AND AFFILIATIONS: (Instructions)
10Enter text here.
11
12KEYWORDS: (Instructions)
13Enter text here.
14
15SUMMARY: (Instructions)
16Enter text here.
17
18ABSTRACT: (Instructions)
19Enter text here.
20
21INTRODUCTION: (Instructions)
22Enter text here.
23
24PROTOCOL: (Instructions)
25Enter text here (10-page maximum, 2.75 pages of highlighted text for filming).
26
27REPRESENTATIVE RESULTS: (Instructions)
28Enter text here.
29
30FIGURE AND TABLE LEGENDS: (Instructions)
31Enter text here.
32
33DISCUSSION: (Instructions)
34Enter text here.
35
36ACKNOWLEDGMENTS: (Instructions)
37Enter text here.
38
39DISCLOSURES: (Instructions)
40Enter text here.
41
42REFERENCES: (Instructions)
43Enter text here.
44

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45General formatting
46 The manuscript must be submitted as an editable .doc or .docx file.
47 Use 12 pt, Calibri. Do not underline any text in the protocol; however, bold text is acceptable.
48 Include single-line spaces between all paragraphs, headings, steps, etc.
49 Use a standard US Letter page size with 1-inch (2.54 cm) margins on all sides.
50 The manuscript text must be original, in complete sentences, and in paragraph form.
51 Define acronyms/abbreviations upon first use in the main text.
52 Use SI units as much as possible and abbreviate all units: L, mL, µL, cm, kg, h, min, s, etc.
53 Include a space between all numbers and the corresponding unit: 50 mg, 100 mL, 37 °C, etc.
54 Abbreviate species names after first use: Caenorhabditis elegans should be C. elegans.
55
56TITLE: (150-character maximum)
57The title should represent the content included in the video and include the model system
58used or the type of study design. Avoid the use of abbreviations.
59
60AUTHORS AND AFFILIATIONS:
61 Provide full unabbreviated affiliations and institutional email addresses for all authors.
62 Affiliations should reflect where the work was performed.
63 Please see our editorial policies: (www.jove.com/author/editorial-policies) for more details
64 regarding authorship. Indicate equal contributions or other footnotes with asterisks (*).
65 The published corresponding author may differ from the one used for correspondence with
66 the editorial office during the submission and publication process.
67
68 Jane A. Smith1,*, Edward A. Jenner1,2,*
69
70 1
Department of Biology, Harvard University, Cambridge, MA, USA
71 2
Department of Neuroscience, Oxford University, Oxford, UK
72
73 *These authors contributed equally.
74
75 Email addresses of co-authors:
76 Jane A. Smith (jasmith@harvard.edu)
77
78 Corresponding author:
79 Edward A. Jenner (EAjenner@ox.ac.uk)
80
81KEYWORDS: (6 minimum, 12 maximum)
82
83SUMMARY: (10-word minimum, 50-word maximum)
84The summary should clearly state the goal of the protocol. It may include a general description
85of the method and its applications. This description should focus on the protocol, not on the
86results obtained by the method.

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 87ABSTRACT: (150-word minimum, 300-word maximum)
88The abstract should focus on the method being presented rather than on the results of a
89specific experiment. It should include a statement about the purpose of the method. A detailed
90overview of the method and a summary of its advantages, limitations, and applications are
91appropriate here. Focus on the general types of results acquired. Do not include references
92here.
93
94INTRODUCTION: (150-word minimum, 1,500-word maximum, 2-paragraph minimum)
95This section should include:
96 A clear statement of the purpose of this method
97 The rationale behind the development and/or use of this method
98 The advantages over alternative methods with references to relevant studies
99 The context of the method in the wider body of published literature
100 Information to help readers decide whether the method described is appropriate for them
101
102PROTOCOL: (1-page minimum, 10-page maximum)
103The protocol text should provide a detailed description to enable the accurate replication of
104the presented method (including setup, materials, actions, conditions, etc.) by both experts
105and researchers new to the field. Well-established methods (e.g., western blotting, PCR,
106spectrophotometry) used within the protocol should be cited as necessary and any
107modifications of the aforementioned procedures should be described.
108
109A) Format
110 The protocol must be a numbered list: step 1 followed by 1.1, followed by 1.1.1, etc. Each
111 step should include 1−2 actions and contain 2−3 sentences. Use subheadings and substeps
112 for clarity if there are discrete stages in the protocol.
113 Include a space between each numbered step or note in the protocol.
114 Short notes (“NOTE:”) can be used sparingly to describe nonactionable items. Discussion of
115 the protocol can be moved to the representative results or discussion section.
116 Indicate any points at which the experiment can be paused and then restarted later. For
117 these situations, indicate the choices at that point in the protocol.
118 Indicate any toxic or harmful chemicals with the word “CAUTION” when they are first used
119 and include notes that describe the hazard and the appropriate handling guidelines.
120 All methods that involve the use of human or vertebrate subjects and/or tissue sampling
121 must include an ethics statement at the beginning of the protocol section.
122 For actions involving software usage, provide all specific details (e.g., button clicks, software
123 commands, any user inputs, etc.) needed to execute the actions.
124
125B) Grammar
126 Use the active/imperative voice and complete sentences throughout the protocol.
127 Avoid the use of personal pronouns (e.g., I, you, your, we, our) or colloquial phrases.
128 Avoid the use of commercial language, including ™/®/© symbols or company brand names
129 before/after an instrument or reagent. Cite these in the Table of Materials instead.

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130 List all centrifugation speeds in terms of centrifugal g-force instead of rpm: 100 x g
131C) Protocol length and highlighting
132 There is a 10-page upper limit (with proper formatting). There is a 2.75-page upper limit on
133 the amount of content we can film for a single video article.
134 For a protocol section that exceeds 3 pages, highlight in yellow up to 2.75 pages (no less
135 than 1 page) of protocol text (including headers and spacing) to be featured in the video.
136 Our scriptwriters will derive the video script directly from the highlighted text.
137 Bear in mind the goal of the protocol and highlight the critical steps to be filmed.
138 Ensure that the highlighted steps form a cohesive narrative with a logical flow from one
139 highlighted step to the next. The highlighted text must include at least one action that is
140 written in the imperative voice per step. Do not highlight notes.
141 There is no need to delete steps from the protocol. The full-length manuscript will be
142 published along with the video. All unfilmed steps will still be available in the written
143 manuscript.
144
145D) Equations (example: www.jove.com/video/58316)
146 Ensure that all inline equations are formatted identically, using a consistent font style.
147 Separate each equation to its own line and define all terms in the equation.
148 Do not embed equations as images. Instead, use the equation builder in MS Word.
149
150E) Example protocol
151
1521. Deactivation of basic alumina
153
1541.1. To generate basic alumina (activity IV), add 100 g of basic alumina (activity I) to a 500 mL
155round-bottom flask.
156
1571.1.1. Add 10 mL of water to the flask and fit it with a glass stopper.
158
1591.1.2. Shake the flask vigorously until no lumps are visible, indicating the even spreading of
160water throughout the alumina. Allow the alumina to cool to room temperature (RT).
161
162CAUTION: Adsorption of water is exothermic, and the flask may get hot, which may result in a
163buildup of pressure. Release any pressure buildup frequently.
164
1652. Purification of the iodoaziridine
166
1672.1. Purify the crude iodoaziridine by column chromatography using basic alumina (activity IV)
168as the stationary phase, eluting with hexane and grading to 5% EtOAc/hexane 7.
169
1702.2. Combine the product-containing fractions and remove the solvent under reduced pressure
171to obtain the pure iodoaziridine.
172

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173NOTE: The protocol can be paused here.

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174REPRESENTATIVE RESULTS: (example: www.jove.com/video/55733)
175Provide a concisely written description of a representative outcome following the use of this
176protocol, so that a viewer will have a sense of a “positive” and/or “negative” result. Reference
177all data and figures in the manuscript, emphasizing how the results confirm the success of the
178protocol and how to interpret the data. Include data from successful experiments and data
179from suboptimal experiments to demonstrate the range of outcomes possible. Also, include
180results for possible outcomes if critical steps are not followed. A diagram/schematic of the
181method is recommended but is NOT sufficient.
182
183 All claims of the effectiveness of a method must be supported with data (i.e., representative
184 results). For example, if a method is claimed to purify nuclear envelope proteins from a cell,
185 a figure definitively demonstrating this purification must be included. The manuscript must
186 include at least 1 figure or table providing representative results.
187
188 Provide a separate file for each figure and table; do NOT embed figures or tables within the
189 manuscript document. The default placement for all figures and tables in the final
190 publication is below the representative results’ text. Indicate via brackets if figure/table
191 placement at another location in the text is preferred [Place Figure 1 here].
192
193 If a figure is adapted or republished from a previous publication, cite the original article in
194 the figure legend. Reprint permission for the previously published/adapted figure is
195 required.
196
197Example: This figure has been modified from Zhang et al.1.
198
199FIGURES AND TABLES:
200 Ensure that all details are visible and that formatting is consistent throughout. Avoid
201 unnecessary figures and panels.
202 All figures should be provided as individual files. Multipanel figures (A, B, C, etc.) should be
203 submitted as a single image file that contains the entire figure.
204 Use capital letters for panel identification. Label the panels at the upper left corner
205 whenever possible.
206 All microscopic images must include scale bars, ideally at the lower right corner.
207 All data figures must include measurement definitions and error bars (if applicable). Define
208 all error bars (SEM, SD, range, etc.).
209 Preferred figure file types: .ai, .eps, .psd, .svg, .pdf
210 .tiff and .jpg (not preferred) files must be at least 1,440 pixels x 480 pixels, or 300 dpi.
211 Preferred animated figure file types: .mov, .mp4, .m4v (upload as “Video or Animated
212 Figure”)
213 All tables should be provided as individual .xls or .xlsx files and submitted as tables. Avoid
214 any coloring or formatting in the tables.

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215FIGURE AND TABLE LEGENDS:
216Each figure or table, including supplemental figures/tables, must have an accompanying legend
217comprised of a short title and a short description of each panel or a general description.
218Legends should be included as part of the manuscript and not be included in the figure file.
219
220Example: Figure 1: Representative flow cytometry analysis of nonpermeabilized cells. (A)
221Schematic representation of gating strategy used in flow cytometry analysis sample. Data were
222analyzed after acquisition with the appropriate software … (B) Semi-log graph for the …
223
224TABLE OF MATERIALS:
225A Table of Materials is required for all articles. A template is provided here. Sort the materials
226alphabetically by material name. Do not number the Table of Materials in the article. Do not
227include any ™/®/© symbols here.
228
229SUPPLEMENTARY FILES:
230Supplementary figure files must be referenced in the text. Production files, such as screenshots
231and permissions, can be uploaded as numbered “supplemental files”.
232
233 Upload all scripts and coding files as “Supplemental coding files”.
234
235DISCUSSION: (3–6 paragraphs)
236JoVE is a methods-based journal. Thus, the discussion section of the article should be focused
237on the protocol and not on the representative results.
238
239This section should discuss the following with citations:
240 Critical steps in the protocol
241 Modifications and troubleshooting of the method
242 Limitations of the method
243 The significance of the method with respect to existing/alternative methods
244 Future applications or directions of the method
245
246ACKNOWLEDGMENTS:
247List acknowledgments and all funding sources for the work here.
248
249DISCLOSURES:
250The corresponding author must ensure that all authors have disclosed any and all conflicts of
251interest. An example of a conflict of interest would be “The author [full name] is an
252[employee/shareholder, etc.] of [full company name] that produces reagents and/or
253instruments used in this article.” If authors have no conflict of interest, make this clear by
254stating “The authors have nothing to disclose.”

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255REFERENCES: (10 minimum; if using EndNote, use the JoVE EndNote style file)
256
257A) In-text formatting
258 When giving a reference in the text, the corresponding number from the reference list must
259 appear superscripted without a space after the word/group of words it applies to but before
260 any punctuation. The references should be numbered in order of appearance.
261 Multiple references should be separated by commas or by an en dash for inclusive numbers:
262 example2,5 refers to references 2 and 5, while example2–5 refers to references 2 through 5.
263 Personal communications, unpublished data, and conference abstracts can be cited
264 parenthetically in the text with the author’s last name, initials, and the year.
265 Footnotes should not be used. Grant details and personal acknowledgments should not be
266 cited as a numbered reference (but included in the acknowledgments section).
267
268B) Citation formatting
269 Last name, first and middle initials (if available). List ALL authors. If there are six or more
270 authors, list the first author and then “et al.”.
271 “Accepted” or “In Press” can be listed after the title or journal name and before the year.
272 Manuscripts that are in preparation or under review should not be listed.
273
274C) Examples
275 Journal article: Bedford, C. D., Harris, R. N., Howd, R. A., Goff, D. A., Koolpe, G. A.
276 Quaternary salts of 2-[(hydroxyimino)methyl]imidazole. Journal of Medicinal Chemistry. 32
277 (2), 493–503 (1998).
278 Book: Kioh, L. G. et al. Physical Treatment in Psychiatry. Blackwell Scientific Pubs. Boston,
279 MA (1988).
280 Book chapter: Guo, A., Zhu, X.-Y. The critical role of surface chemistry in protein
281 microarrays. In Functional Protein Microarrays in Drug Discovery. Edited by Predki, P. F., 53–
282 72, CRC Press. Boca Raton, FL (2007).
283 Webpage: Rueden, C., Schindelin, J., Hiner, M., Eliceiri, K. SciJava Common [software].
284 http://scijava.org (2016).
285 Conference proceedings: Patrick, C. L. Multi-heterodyne spectroscopy using Fabry-Perot
286 interband cascade lasers for trace gas detection: a feasibility assessment. Proceedings of
287 SPIE OPTO 2017: 10123, Novel In-Plane Semiconductor Lasers XVI. San Francisco, CA (2017).
288 Thesis: Tessmann, J. Neuroendocrine Genomics for Tumor Variant Discovery. Doctoral
289 dissertation. University of Iowa (2018).
290 Patent: Rosell Ferrer, F. X., Sánchez Terrones, B., Bragós Bardia, R., Bayés Genís, A., Llucià
291 Valldeperas, A. Methods and devices for mechanical and electrical stimulation of stem cell
292 monolayer and 3d cultures for tissue engineering applications. WO/2013/185818.
293 Barcelano, Spain (2013).

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