Certificate of Analysis: Lyophilized Microorganism Specification and Performance Upon Release

Specifications Expiration Date: 2020/5/31

Microorganism Name: Escherichia coli Release Information:
Catalog Number: 01265 Quality Control Technologist: JoAnn E Schmitz
Lot Number: 1265-01** Release Date: 2018/6/21
Reference Number: NCTC 13353
Purity: Pure
Passage from Reference: 2

Performance
Macroscopic Features: Medium:
Small, circular, low convex, entire margin, grey, and smooth. SBAP
Microscopic Features: Method:
Gram negative rods. Gram Stain (1)
ID System: MALDI-TOF (1) Other Features/ Challenges: Results
See attached ID System results document. (1) Oxidase (Kovacs): negative
Beta-glucuronidase (E. coli Broth w/MUG): positive
AST-GN Cefepime: >=64 - Resistant

Amanda Kuperus
Quality Control Manager
AUTHORIZED SIGNATURE
**Disclaimer: The last digit(s) of the lot number appearing on the product label and packing slip are merely a packaging event number. The lot number displayed on this
certificate is the actual base lot number.

Note for Vitek®: Although the Vitek® panel uses many conventional tests, the unique environment of the card, combined with the short incubation period, may
produce results that differ from published results obtained by other methods.

Refer to the enclosed product insert for instructions, intended use and hazard/safety information.

Individual products are traceable to a recognized culture collection.

(1) These tests are accredited to ISO/IEC 17025:2005.

© 2012 Microbiologics, Inc. All Rights Reserved. 200 Cooper Avenue North Saint Cloud, MN 56303 Page 1 of 1 DOC.286
Bruker Daltonik MALDI Biotyper Classification Results

Meaning of Score Values

Meaning of Consistency Categories (A - C)

Sample Name: Escherichia coli

Sample Description: 01265
Sample ID: 1265-01
Sample Creation Date/Time: 2018-06-13T16:15:49.560 AS/CC
Applied MSP Library(ies): BDAL, Mycobacteria Library (bead method), Filamentous Fungi Library 1.0, Listeria

Sample Name Sample ID Organism (best match) Score Value

C5
1265-01 Escherichia coli 2.52
(+++) (A)

Comments:
closely related to Shigella / Escherichia fergusonii and not definitely distinguishable at the moment