OFFICIAL ABSTRACT and CERTIFICATION

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Krizia Nicole R. Dequilla, Denise Danielle B. Dilag, Verny Jane D. Hucalinas
Doña Montserrat Lopez Memorial High School
Animal Sciences o

The purpose of this research is to prove that the extract from Bakhaw lalaki
Behavioral & Social o
(Rhizophora apiculata) leaves is effective against pathogenic bacteria. The

Sciences
bacteria used for the antibacterial sensitivity testing were Escherichia coli and Biochemistry o
Staphylococcus aureus. The methods of this study includes the preparation of

Biomedical & Health
leaves; extraction of leaves (pulverized leaves was packed into a soxhlet Sciences o

apparatus and extracted with 100 mL of 70% methanol); rotary evaporation; Biomedical
phytochemical analysis; determination of minimum inhibitory concentration (MIC) Engineering o

and minimum bactericidal concentration (MBC); sensitivity testing of Cellular & Molecular
microorganisms, the positive control used was ciprofloxacin while methanol and Biology o

distilled water were used for negative. (Kirby-Bauer Disc Diffusion Method was Chemistry o

used in the analysis); the plates were then carefully inverted and incubated for 24 Computational
hours at 37°c. After the incubation, a ruler was used to measure the diameter of Biology &
the zone of inhibition. The measurement obtained from the individual discs were Bioinformatics o

recorded and compared with the standards table to determine the sensitivity zone Earth & Environmental o

to determine whether the tested bacterial species is sensitive to the extract. With Sciences
values <8 mm were considered as not active against microorganisms. The mean Embedded Systems o

of the inhibition zone of E. coli is 13 mm while S. aureus is 14 mm. Since the Energy: Chemical o

results are greater than 8 mm and considered as active against microorganisms, Energy: Physical o

the study revealed that crude methanolic extract from Bakhaw lalaki (Rhizophora Engineering Mechanics o

apiculata) leaves has antibacterial property. Environmental
Engineering o
1. As a part of this research project, the student directly handled, manipulated, or

Materials Science o

interacted with (check ALL that apply):

Mathematics o

o human participants ■ potentially hazardous biological agents
o Microbiology o

o vertebrate animals ■ microorganisms
o o rDNA o tissue Physics & Astronomy o

Plant Sciences o
■
2. I/we worked or used equipment in a regulated research institution ■ Yes
o o No

Robotics & Intelligent o

or industrial setting:

Machines

Systems Software o
3. This project is a continuation of previous research. o Yes ■ No
o

Translational Medical o



Sciences

4. My display board includes non-published photographs/visual ■ Yes
o o No


depictions of humans (other than myself):

5. This abstract describes only procedures performed by me/us, o■ Yes o No

reflects my/our own independent research, and represents one year’s
work only


6. I/we hereby certify that the abstract and responses to the ■ Yes
o o No


above statements are correct and properly reflect my/our own work.

This stamp or embossed seal attests that this project is in compliance with all federal
and state laws and regulations and that all appropriate reviews and approvals have
been obtained including the final clearance by the Scientific Review Committee.