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Pathology 5 A PDF
Pathology 5 A PDF
09/13/10
Normal Histology:
Definitions:
Hyperkeratosis Parakeratosis Acanthosis
Papillomatosis Dysplasia
Acanthosis Cytologic atypia:
w/upward 1) Variation in size
projections and shape of nucleus
(wart; (nuclear
seborrheic pleomorphism)
keratosis) 2) Variation in
staining
(hyperchromatism)
3) Increased and
abnormal mitotic
figures (cancer;
precancer)
Solar Elastosis
Seborrheic Keratosis
• Common
• Occur >30 years
• Trunk/face Irritation
• Rarely as paraneoplastic syndrome Inflammation
w/an internal malignancy.
– Sign of Leser-Trelat
• Papules – raised.
• Smooth, rough, or “greasy” surface
• Light to dark brown
• “Stuck on” appearance ^ Solitary ^ Multiple
• People often scratch them off.
Above: Dermatosis
Papulosis Nigra – Multiple
seborrheic keratoses.
Actinic Keratosis
• Fair-complexioned individuals (Irish/Anglo-Saxon
heritage)
• Chronically sun-exposed areas
• Induced by ultraviolet radiation
• Course
– stable
– regress
– progress to squamous cell carcinoma
• Macules - flat
• Papules - raised
• Scale
• Hyperkeratosis (increased stratum corneum); Test q: A 58y/o farmer has had a lesion on his face that has been enlarging for
the past 3yr. This lesion is excised, and microscopic exam shows basal cell
parakeratosis hyperplasia. Some of the basal cells show nuclear atypicality associated
• Dysplasia of lower epidermis w/marked hyperkeratosis and parakeratosis. Solar elastosis is present. Which of
• Solar elastosis the following lesions best accounts for these findings? Actinic keratosis.
Test q: A 50y/o male presents w/a maculopapular scaly lesion on the back of his
neck. A biopsy shows dysplasia of the keratinocytes in the lower epidermis and
marked solar elastosis in the dermis. Diagnosis: actinic keratosis.
• Hyperkeratosis; parakeratosis
• Acanthosis
• Dysplasia of entire epidermis – large
nuclei within the entire epidermis
• Extension of tumor into dermis
• Squamous “pearls”
• Solar elastosis
Cells still make keratin – result is keratin pearls. Have s. corneum but it’s trapped beneath rather than being at the
surface of the skin.
Test q: A 22y/o male develops more than a dozen basal cell carcinomas on his upper face. This syndrome is autosomal dominant and is associated
w/mutation of which gene? PTCH.
Test q: A 5y/o male presents w/a basal cell carcinoma on his forehead. The genetic basis for this disease is: PTCH.
Test q: A 66y/o female farmer has a pearly nodule on her forehead. The nodule is focally ulcerated and shows prominent dilated blood vessels.
Histologically you would expect to see: peripheral palisading.
Test q: All of the following regarding basal cell carcinoma are true except: Tumor exhibits full thickness atypia of the epidermis (Other choices –
Retraction spaces are seen around tumor lobules; Peripheral palisading is a feature; The nose is a frequent site; Sun exposure is a risk factor)
Keratoacanthoma
• Benign vs. malignant
• Rapidly growing tumor
• M>F
• Sun-exposed skin
• May spontaneously involute (regress)
• Rarely aggressive
• Cup-shape
• Keratin plug
• Glassy keratinocytes
Test q: A 70y/o man develops a keratoacanthoma on his nose. The lesion is cup-
shapes and filled w/glossy keratinocytes. The lesion can be expected to:
Spontaneously regress.
Test q: A 60y/o man has noted the appearance of a nodule on his ear during the past
month. On phys exam, there is a 1.2cm flesh-colored, dome-shaped nodule on his
right ear lobe. The nodule has a central keratin-filled crated surrounded by
proliferating epithelium. The lesion regresses and disappears within 1mo. Which of
the following is the most appropriate diagnosis of this lesion? Keratoacanthoma.
Adnexal Tumors
• Most benign
• Derived from adnexal structures including follicle, sebaceous gland, eccrine gland, apocrine gland
Cylindroma
• Benign adnexal tumor, derived from eccrine glands
• Occurs on forehead and scalp
• Multiple lesions may coalesce into hat-like growth (“turban
tumor”)
Melanocytic Tumors:
Nevus
• Nests of cells
– epidermis (junctional nevus)
– dermis (intradermal nevus)
– epidermis + dermis (compound
nevus)
• Nevus cells
– round with oval nucleus
– variable melanin
• Summary of histologic features
– Symmetric
– Predominant nest pattern
– Cytologically bland melanocytes
– No dermal mitotic figures
– “Maturation” (nuclei get smaller
with descent into dermis)
Test q: Characteristics of benign nevi include:
maturation; smaller cells in dermis.
Congenital Melanocytic Nevi: Dysplastic Nevi:
• Macules and papules
• Irregular: shape, border, color
• >5mm diameter
• Occur on sun-exposed and non-
sun-exposed skin
• Clinical significance unclear:
– Isolated lesions: not very
significant
– Multiple lesions: probably
increased risk of melanoma
(only very slightly)
– Dysplastic nevus syndrome
(atypical mole syndrome)
• Autosomal dominant
Malignant Melanoma:
Test q: In the US, the malignant tumor most commonly causing death in females
is: Lung.
Test q: The malignancies responsible for the most cancer deaths in women and
men in the US (currently) is (are): Lung for both
Test q: In the US, the highest incidence of melanoma can be found in: Texas
(other choices: CA, ME, NC, IN. Proximity to equator)
Test q: Malignant neoplasms develop in patients w/xeroderma pigmentosum
because these patients: Lack an enzyme necessary for DNA repair.
Melanoma
• Not just a disease of older patients
– Most common cause of cancer-related mortality in young women
– Well documented pediatric cases
• Warning signs
– Change in pre-existing mole
– Itching/pain in pre-existing mole
– New pigmented lesion as adult
– ABCDs
Test q: In melanoma, the single most important prognostic factor is: depth of
invasion
Test q: The Breslow measurement is used to: assess melanoma tumor
stage and prognosis (as opposed to tumor grade and prognosis)
Clark’s Levels
I – tumor cells are confined to the epidermis (melanoma in situ)
II – superficial papillary dermis
III – filling papillary dermis
IV – in reticular dermis, where collagen fibers are thicker (distinguishes from papillary
dermis)
V – has the greatest risk of metastasis.
Test q: A 37y/o red-haired female has a pigmented papule on her back that measures 0.8cm in diameter. On excisional biopsy, melanoma is
diagnosed. The melanoma is confined entirely to the epidermis. The Breslow measurement is .75mm. This melanoma is most accurately described as:
Melanoma in situ REPEATED x2 (once – it was 1.0cm in diameter)
Test q: A 39y/o woman has a nodule on her back that has become larger over the past 2mo. On phys exam, there is a 2.1cm pigmented lesion
w/irregular borders and an irregular brown-black color. An excisional biopsy w/wide margins is performed and microscopic exam of the biopsy specimen
shows a malignant melanoma composed of epithelioid cells that extends 3.5mm (one year, was “2mm”) down to the reticular dermis. There is a band of
lymphocytes beneath the melanoma. Which of the following statements is most appropriate to make to this patient regarding these findings?
Metastases are probable. REPEATED x2 (One year, answer was “The prognosis is poor.” Other choices were the same both times: Her immune
system will prevent metastases; Other family members are at risk for this condition; The primary site for this lesion is probably the eye; Nevi elsewhere
on her body will become malignant.)
Breslow Level:
• Perpendicular distance from granular layer
• Measured in mm
• More reproducible
Pagetoid spread.
Melanoma – Molecular mechanisms: Test q: A 20y/o man has a raised, irregular, pigmented lesion
• Deletions of CDKN2A (INK4A) on his forearm that has increased in size and become more
– Deletion of p16 protein product leads to: irregular in color over the past 4 months. An excisional biopsy
specimen shows a malignant melanoma that extends into the
• Unrestricted phosphorylation of Rb (inactive Rb) reticular dermis. Family history indicates that the patient’s
• Unrestricted cell growth paternal uncle died of metastatic melanoma that spread to the
– Deletion of p14 (alternative protein product) leads to liver after excision of a primary lesion on the foot. His
• Decreased p53 function (via decreased function of MDM2) grandfather required enucleation of the left eye because of a
“dark brown” mass in the eyeball. Which of the following
• Enhanced growth of altered cells genes is most likely to have undergone mutation to produce
• BRAF mutations these findings? p16 (cell cycle inhibition) REPEATED x2
– Found in melanomas and nevi
– Part of RAS/RAF/MAP kinase pathway
Mesenchymal Tumors:
Benign Fibrous
Histiocytoma(BFH)/Dermatofibroma (DF)
Clinical Features:
• Solitary, slow-growing nodule, often red-brown in
color
• Usually on extremities in dermis or superficial
subcutis Dermatofibroma. Large dark pink
• Presents in early or mid-adult life area = lesion. Proliferation induces
• Spindled tumor cells acanthosis (thickening) of the
• Collagen trapping – at the periphery of the overlying epidermis.
tumor, the tumor cells interdigitate around
preexisting collagen fibers of the reticular dermis
DFSP: White spaces = what is left of the subcutaneous fat. Below: cells are a bit amorphic. Can see mitoses.
Hematopoietic Tumors:
Mycosis Fungoides:
• Uncommon (<1% of lymphomas)
• Older adults
• Clinical progression in disease:
patch plaque nodule
• Patch Stage: Scaly, reddish plaques that arise Patch stage Plaque stage Plaque stage
mainly on clothed areas of the body – sun
exposure actually acts as a treatment.
• T cell lymphoma – uncommon relative to
systemic lymphomas, but one of the most
common skin lymphomas. Some people never
progress through the disease. Nodule (Tumor) Stage
Not everyone evolves to this
stage – some people are in
Cutaneous T-cell Lymphoma patch stage their whole life.
(Mycosis Fungoides)
Sezary Syndrome
(Erythrodermic Stage)
Langerhans Cell Histiocytosis (Histiocytosis X): Langerhans cell = APC typically found in epidermis
• Children > adults
• Solitary or multiple
• Papules, nodules or plaques
• Proliferation of Langerhans cells
General Principles:
• Thousands of inflammatory dermatoses
• Limited number of histologic reaction patterns
• Significant histologic overlap
• Correlation of histologic features with clinical presentation essential
• Dermatopathologist dependent on history and clinical description of lesions by dermatologist or family practitioner
Histologic Classification:
Based on “pattern” of inflammatory changes. Some patterns:
– Spongiotic (intraepidermal edema) ex: contact dermatitis/eczema
– Psoriasiform (epidermal acanthosis) ex: psoriasis
– Interface (damage to basal layer)
• Perivascular (inflammatory cells predominant ly around blood vessels) ex: erythema multiforme, lupus
• Lichenoid (inflammatory cells arranged as dense band beneath epidermis) ex: Lichen Planus
Other patterns:
– Perivascular infiltrate (no epidermal changes)
– Interstitial infiltrate (inflammatory cells between collagen bundles)
– Bullous disease (blister)
• Intraepidermal – blister within epidermis
• Subepidermal – blister beneath epidermis
– Panniculitis (inflammation in subcutaneous fat)
– Miscellaneous
Patterns:
• Represent starting point (defines category)
• Individual differentiating microscopic features
• Correlation with clinical features often necessary to make final diagnosis
Spongiotic Dermatitis
• Primary feature: intraepidermal edema (ECF as a result of inflammation)
– Accumulation of edema fluid within epidermis
– Keratinocytes pull apart (easy to see intercellular bridges)
– May see intraepidermal vesicles
– May see some acanthosis – thickening – in older lesions (overlap with
psoriasiform pattern)
• Red,papulovesicular, oozing and crusting
• Prototype: contact dermatitis
Eczematous Dermatitis
Histologic Features:
• Spongiotic dermatitis
– Intercellular edema in epidermis
– Small vesicles in epidermis due to edema fluid
– Inflammatory infiltrate in dermis (typically lymphocytes and eosinophils
– clue that it’s an allergic process)
Psoriasiform Dermatitis
• Epidermal acanthosis most important feature
• Variable perivascular infiltrate
• Variable spongiosis
• Prototype: psoriasis
Psoriasis
• Chronic dermatitis
• Affects 1-3% of the population
• May develop at any age; genetic predisposition
• Psoriatic arthritis
– Overall incidence ~5%
– Usually seen in patients with severe skin
disease
• HIV: psoriasis can be presenting sign
• Red plaques with loose silvery scale
• Pitted nails with yellow discoloration
• Frequent sites: extensor surfaces, scalp,
lumbosacral region, gluteal cleft, glans penis
Interface Dermatitis
Key feature: damage to basal layer of epidermis, usually by lymphocytes
• Damage to basal layer of epidermis with perivascular inflammation
Erythema Multiforme
Toxic Epidermal Necrolysis (TEN)
Lupus Erythematosus
• Damage to basal layer of epidermis with lichenoid inflammation (dead span of lymphocytes underneath the epidermis)
• Lichen Planus
Erythema Multiforme:
• Spectrum of disease (EM, TEN)
• EM = less severe end of the spectrum
• TEN = toxic epidermal necrolitis (most severe end of the spectrum)
• Dramatic hypersensitivity response with a wide range of possible causes
– Infections: HSV, mycoplasma, histoplasmosis, coccidiomycosis, typhoid, leprosy
– Drugs: Sulfa drugs, PCN, barbiturates, salicylates, hydantoins, antimalarials
– Malignancies: carcinomas and lymphomas
– Collagen vascular disease: Lupus erythematosus, dermatomyositis, periarteritis nodosa
– Idiopathic ~ 50% of cases
• Clinical lesions variable (multiple forms)
– Papules
– Targetoid lesion (most characteristic)
– Vesicles
– Bullae
Rapid onset of red papules: Target lesions:
Test q: A 25y/o female had a child in daycare that develops diarrhea
which the patient also developed. Both were treated w/trimethaprim –
sulfa methoxazole (Septra). The mother develops a targetoid rash
w/vesicles and bullae. You suspect: Erythema multiforme.
Test q: A 40y/o male AIDS patient is treated w/Septra (trimethaprim
sulfamethoxazole) for Pneumocystis pneumonia. His CD4 count is
<200. He develops a papular rash which eventually shows a targetoid
pattern. A biopsy shows full thickness necrosis of keratinocytes. The
most likely diagnosis is: Erythema multiforme.
Test q: A 28y/o AIDS patient suffers from Histoplasmosis and
Nocardiosis. He is treated w/Itraconazole and Trimethiprim
Above: Central area = necrosis Sulfamethoxazole. He develops vesicles and target-shaped red skin
of the epidermis papules. This history is consistent with: Erythema multiforme.
Perivascular lymphocytes
Test q: Histologic features of Lupus Erythematosus include: interface vacuoles and focal epidermal atrophy
Lichen Planus
• Chronic but self-limiting disease
• Multiple, symmetric lesions on extremities (especially
wrists)
• Pruritic, purple, polygonal papules
• Oral mucosal involvement common (see right)
– oral lesions have lacy, white pattern.
• Dense band-like lymphocytic infiltrate hugs epidermis
• Epidermal changes:
– Hyperkeratosis
– Wedge-shaped hypergranulosis
• Individual necrotic
keratinocytes (circled)
Pemphigus Vulgaris:
• Presents in the 4th-6th decades, M=F
• Autoimmune blistering disease
– IgG against desmoglein 3 in desmosomes
– Desmosomes hold epithelial cells together – loss of cell
adhesion within the epidermis
• Test q: In pemphigus vulgaris, IgG autoantibodies are directed against:
Desmosomes REPEATED x2
• Variants: pemphigus vegetans and foliaceus
• Flaccid, fragile bullae
• Oral mucosa involvement (typically ruptures before you see it
clinically)
Pemphigus vulgaris.
• Intraepidermal, suprabasilar blister
with “tombstone” basal layer
• Mixed dermal inflammatory
infiltrate, often with eosinophils
• Intraepidermal: looks like this
blister is under the whole epidermis,
but note the one layer of cells left
underneath. The rest makes up the
roof of the blister.
Above: In the base of the blister is one layer of the Above: Pemphigus Vulgaris Immunofluoresence
epidermis. The rest makes up the roof of the blister.
Bullous Pemphigoid:
• Generally affects elderly patients
• Bullae on extremities, intertriginous areas, abdomen and oral mucosa (1/3
pts)
• Autoimmune disease
• IgG against hemidesmosome proteins
• Hemidesmosomes bind epidermis to basement membrane
• Subepidermal blistering process – blisters have more strength because of
more material in the roof. Blisters are tense and stay intact longer (as
compared to fragile blisters of PV, described above).
• Tense bullae
Above: Bullous Pemphigoid. Subepidermal Above: Blister contains edema Bullous Pemphigoid: Immunofluorescence.
blister without “tombstones” or acantholysis. fluid and eosinophils. IgG bound to hemidesmosome – located in
No epidermis in the base of the blister. basement membrane.
Dermatitis Herpetiformis
• Vesicular dermatosis not bullous
rd th
• 3 and 4 decades, M>F
• Pruritic, burning vesicles, especially on extensor surfaces of extremities
• Strongly associated with celiac disease – everyone w/DH either has Celiac disease or sub-clinical Celiac disease.
• Responds to gluten free diet (like celiac)
• IgA deposited in dermal papillae of skin
Incredibly itchy – patients will scratch blisters DH Histologic Features- Subepidermal vesicles Neutrophilic microabscess
before you ever see them in clinic.
Test q: A person w/dermatitis herpetiformis should avoid what food substance: gluten.
Test q: A 35y/o man has had an outbreak of pruritic lesions over the extensor surface
of the elbows and knees during the past month/ He has a history of malabsorption
that requires him to eat a special diet, but he has had no previous skin problems. On
phys exam, the lesions are 0.4-0.7cm vesicles. A 3mm punch biopsy of one of the
lesions over the elbow is performed. Microscopic exam of the biopsy specimen shows
accumulation of neutrophils at the tips of dermal papillae and formation of small
blisters due to separation at the dermo-epidermal junction. Immunofluorescence
studies performed on this specimen show granular deposits of IgA localized to tips of
dermal papillae. Lab studies show serum anti-gliadin antibodies. Which of the
following is the most likely diagnosis? Dermatitis herpetiformis.
DH Immunofluorescence.
Test q: Neutrophilic microabscesses and fluffy IgA deposits on basement membrane
IgA deposits recruit in neutrophils.
anchoring febrils are characteristic of: Dermatitis herpetiformis.
Dermatophytosis:
• AKA: Tinea (capitis – scalp, corporis – body, barbae – beard area, cruris – groin, pedis – foot), “ringworm”
• Dermatophytes infect outer keratin layer of skin
Dermatophytosis
Warts (verrucae):
• Benign neoplasms caused by human papillomavirus (HPV)
– >60 subtypes
– Certain subsets associated w/squamous cell carcinoma
• Common
– Common wart (verruca vulgaris)
– Plantar/palmar wart - caused by diff HPV subtypes
– Genital wart (condyloma acuminatum)
– Flat wart
• Occur at any age
• Self-limited
Test q: A 35y/o man has noted a bump on his upper trunk for the past 6wk. On phys exam, there is a solitary 0.4cm flesh-colored nodule on the upper
trunk. The dome-shaped lesion is umbilicated, and a curd-like material can be expressed from the center. This material is smeared on a slide, and
Giemsa stain shows many pink, homogenous, cytoplasmic inclusions. The lesion regresses over the next 2mo. Which of the following infectious agents
most likely produced this lesion? Molluscum contagiosum
Impetigo:
• Common superficial infection
• Staphylococcus or streptococcus
• Exposed surfaces
• Characteristic honey-colored crust
• Histologic Feature: Subcorneal pustule
• A lot of neutrophils in the stratum corneum –
can see gram positive cocci here.
Scabies like to burrow underneath the stratum corneum. Find them on the penis and web spaces of the fingers.