RESULTS

The Gram stain involves applying a sample from an infected area or a sample of
bacteria grown in culture onto a glass slide. The slide is then treated with a special
stain and examined under a microscope. The stain and shape of the bacteria help
classify which general types of bacteria are present.

First specimen: The resulting stain is colored red, which denotes that it is Gram-negative.
This indicates that it is more dangerous as disease organisms since their outer membrane is
often hidden by a capsule or slime layer which hides the antigens of the cell and the body
recognizes foreign bodies by its antigen. It has forms of very short rods and has fading red
color on the top and in the middle. The bacterium is a Facultative intracellular bacteria where
it invade host cells when it gives them selective advantage.
 Second specimen: The bacteria seen through the microscope above has a resulting stain
of pink in color, which indicates that it is also a Gram-negative and its cell membrane is thin
but difficult to penetrate and because of this, they are often resistant to antibiotics and other
antibacterial interventions. It has a form of small figure and has different patterns which are
tiny dots and lines that are between sphere and rod-shape. It is also a facultative and an
intracellular bacterium which is a bacteria that is capable of living and reproducing either
inside or outside cells.

CONCLUSION
The purpose of having this experiment, Gram Stain, is to differentiate two large
groups of bacteria based on their different cell wall constituents. We distinguished it by
telling if it is Gram positive or Gram negative when we coloured the cells red or violet. As
read in the methodology, we used all the materials indicated and followed all the steps for us
to have a successful experiment. We were able to achieve and complete our objective of
determining the peptidoglycan layer of the bacteria in the cultured media. This will also help
us to determine what kind of microorganisms live in the cultured media.