ATIVIDADE EXPERIMENTAL BIOLOGIA N.

º3 – IDENTIFICAÇÃO DE BIOMOLÉCULAS
Nome: ______________________________________ nº______ Avaliação:________________

I -. Problem: Which organic and inorganic compounds can be found in milk, orange juice and potatoes?

II. Materials:

III. Procedure:

Part I (Testing for Monosaccharides)

1. Obtain 3 clean test tubes and place them in a test tube rack, mark each one with the letters A, B and C.

2. Fill: test tube A with 2 mL of milk; test tube B with 2 mL of orange juice; test tube C with 2 mL of potato juice.

3. Add 5 mL of fehling’s solution

4. Heat each tube with an alcohol burner.

5. Record any colour changes

Part II (Testing for Starch)

1. Obtain 3 clean test tubes and place them in a test tube rack, mark each one with the letters A, B and C.

2. Fill: test tube A with 2 mL of milk; test tube B with 2 mL of orange juice; test tube C with 2 mL of potato juice.

3. Add 3 drops of Iugol’s solution to each test tube.

4. Let the test tubes sit for 3-5 minutes.

5. Record any colour changes

Part III (Testing for Proteins)

1. Obtain 3 clean test tubes and place them in a test tube rack, mark each one with the letters A, B and C.

2. Fill: test tube A with 2 mL of milk; test tube B with 2 mL of orange juice; test tube C with 2 mL of potato juice.

3. Add Biuret reagente ( 4 drops of copper sulphate 5%+ 1 drop of potassium hydroxide 50%) to each test tube.

4. Gently shake the contents of each test tube.

5. Let the test tubes sit for 3-5 minutes.

6. Record any color changes.

Part IV (Testing for Lipids)

1. Obtain 3 clean test tubes and place them in a test tube rack, mark each one with the letters A, B and C.

2. Fill: test tube A with 2 mL of milk; test tube B with 2 mL of orange juice; test tube C with 2 mL of potato juice.

3. Add 5 drops of sudan III to each test tube

4. Gently shake the contents of each test tube.

5. Let the test tubes sit for 3-5 minutes.

6. Record any color changes.

Part V (Testing for water)

1. Obtain 3 clean test tubes and place them in a test tube rack, mark each one with the letters A, B and
C.

2. Fill: test tube A with 2 mL of milk; test tube B with 2 mL of orange juice; test tube C with 2 mL of potato
juice.

3. Add a bit of anhydrous copper sulfate

4. Record any color changes.

V. Results:

(DRAW A TABLE)
 ANEXO

ALGUNS REAGENTES QUÍMICOS UTILIZADOS EM LABORATÓRIO PARA IDENTIFICAÇÃO DE SUBSTÂNCIAS

Substância a identificar Reagente Reacção característica

O sulfato de cobre anidro toma a

Água Sulfato de cobre anidro
cor azul

Ião cloro Nitrato de prata Precipitado branco que escurece

quando exposto à luz

Ião potássio Ácido pícrico concentrado Precipitado, em agulhas

Ião cálcio Oxalato de amónio Precipitado branco

Ião sulfato Cloreto de bário Precipitado branco

Glicose Licor de Fehling Precipitado cor de tijolo

Amido Soluto de lugol /água iodada Cor azul arroxeada/azul

Lípidos Sudão III Cor vermelha intensa

Vitamina C Solução de indofenol Descoloração da solução de

indofenol

Proteínas Ácido nítrico e amónia

Cor amarela que passa a cor-de-
laranja (reacção xantoproteica)

Sulfato de cobre e soda cáustica Cor violeta (reacção do biureto)