UREA KIT (DAM method) Forte deamination of Ue in sum pasa and ine (Forinvite Dagrosc Use Ory) Summary Ut ste ond prosuct of the pean metabolen. is syntedeed nto er om be amon proguce bythe fatale fama aces. anspor th le 2 the ney fom where tis exceed Increased ves ae found in ronal dsoass, inary obstuctns, shock, sengastv hea fale and buns. Decreased oes ‘ound nero and regnaney Principe ca in a1 acide medium condenses with Dace rrenoxime at 100 to for rad eooued corolex. Inensty of he colurfrmedsdrecsy propria fhe amouniofueapresertinthesarpe oe Urea iceymonorine ne RedColred Complex Nomalreerence values Serum, Pera ine 440mg uplo2agtite Is recomended tat each boar ata is own raalrangs representing tsar population Contents asteste —s0Tete Li: Urea Reagent Taml——450mi (asnciReoger T5nl 450m LE: DaNRoagert 75m 480m 5 :UreeSiancan (40mg) Sal Sal Strapestabilty Agger are sible at RT. ‘holaes, pry morsoned on Reagent Preparation Reagors ae reag ose. Do nol pate wih mou, Sample materia Serum, plasma or Utne. Uine should be of 24 hous tallecion. Dilze the urine specimen 1:20 with distleldeonises waar bela the assay Ure reported {obedable enamferSdayeat 2€C. Procedure Wiveorath te 20am Hg 48m) reer Teperaie ‘0 Lorteat tem LT Ppt ito clean cry tst bes labled 2s Blank (8), Sanda) andes) ‘tion soot Sequence yo UesReapenttt) | 19 10 40 redReagentt) | 19 40 40 DaMResgerts) | 19 10 t0 Dis water cot oot ao xwetandtoap tees ubesinblig waa 019 minis. col unde naning tap wales and messre the ‘Ssorbancocth Standard (Abs 8), and Testsample(A0s agatha Caleuitons Pot Useinmgid = x 4 Rss ost Une Ueaingtire = x6 ers Ure Nitegen in gl = Vea in glx 0.467 Linearty The procedure ine up 71mg. values exceed hs lint ue te sare win died waar a repeat > 358 Cleat valu using a proper luton art Note Thapresancaafarmenia oesratbtarentistest References Fearon, WR (1899 Beston. 33-802 arin R185. Amer od Teo 31678 \yterga, 0871) Cn Chem. 17: 881‘System Parameters Reacton = End Pont Wavelength: 520m ZeroSeting Reagent Blank Ineub. Temp, : 100% Incub: Time 10m. ResdTime : Nocotread = = Interval = Sample Vol: 01m Reagent Vol. : 300m Standard: K0mgid Factor = React Slope: Ineeasng Linearity": TOmal Unis noid ‘tana Dr. Arno Do Rego Bagh Bamboim Gorpox 20. GOA. 403202, NDIA