Stat Fax 3300 PDF

Stat Fax 3300 ®
Service Manual
©Copyright 2004 Awareness Technology Inc. 01/2008 Doc. 3300 Rev. G

TABLE OF CONTENTS
1. INTRODUCTION ................................................................................................................................... 1
1.1 APPLICATIONS ...................................................................................................................................... 1
1.1.1 Intended Use ................................................................................................................................ 1
1.1.2 Summary of the Instrument .......................................................................................................... 1
1.2 SPECIFICATIONS .................................................................................................................................... 2
1.3 WARNING MARKINGS ........................................................................................................................... 5
1.3.1 Safety Symbols ............................................................................................................................. 5
1.3.2 Safety Terms................................................................................................................................. 5
1.4 SAFETY PRECAUTIONS .......................................................................................................................... 5
2. PRINCIPLES OF OPERATION ........................................................................................................... 8
2.1 POWER REQUIREMENTS ........................................................................................................................ 8
2.2 PARTS AND CONTROLS ......................................................................................................................... 9
2.3 GENERAL LAYOUT.............................................................................................................................. 11
2.3.1 The Chassis ................................................................................................................................ 11
2.3.2 Front Panel Electronics ............................................................................................................. 11
2.3.3 Main PCB .................................................................................................................................. 12
2.3.4 Photometer Mechanism.............................................................................................................. 14
2.3.5 Flowcell ..................................................................................................................................... 17
2.3.6 Valve .......................................................................................................................................... 17
2.3.7 Vacuum Pump and Pump Control Circuit ................................................................................. 17
2.3.8 Temperature Control.................................................................................................................. 18
2.4 PARALLEL INTERFACE ........................................................................................................................ 18
2.5 AUXILIARY AND OPTIONAL EQUIPMENT............................................................................................. 18
2.5.1 Auxiliary Equipment Interface ................................................................................................... 18
2.5.2 Optional Equipment ................................................................................................................... 18
3. SERVICE PROCEDURES................................................................................................................... 19
3.1 OPENING THE INSTRUMENT ................................................................................................................ 19
3.2 CLEANING THE FLOWCELL ................................................................................................................. 21
3.3 FLOWCELL CONFIGURATION .............................................................................................................. 22
3.4 FLOWCELL ALIGNMENT ...................................................................................................................... 24
3.5 FLOWCELL TUBING REPLACEMENT .................................................................................................... 25
3.6 TESTING FOR LEAKS IN THE FLOWCELL ASSEMBLY............................................................................ 32
3.7 FLOWCELL CELL INSERT REPLACEMENT ............................................................................................ 34
3.8 MAIN PCB AND/OR PHOTOMETER REPLACEMENT .............................................................................. 35
3.8.1 Photometer Replacement ........................................................................................................... 35
3.8.2 Main PCB Replacement ............................................................................................................. 37
3.9 VALVE TUBING REPLACEMENT .......................................................................................................... 38
3.10 EPROM REPLACEMENT ................................................................................................................... 39
3.11 FILTER CHANGE PROCEDURE ........................................................................................................... 41
3.12 LAMP REPLACEMENT........................................................................................................................ 44
3.13 VACUUM ADJUSTMENT .................................................................................................................... 46
3.14 EXHAUST FILTER REPLACEMENT...................................................................................................... 47
4. TROUBLESHOOTING........................................................................................................................ 48
4.1 KEYPAD PROBLEMS ............................................................................................................................ 48
4.1.1 No Keys Function....................................................................................................................... 48
4.1.2 A Row or Column of the Keypad Does Not Function................................................................. 48
4.1.3 The Keyboard and Display Do Not Function............................................................................. 48
4.1.4 Keypad Key Stuck ...................................................................................................................... 48
4.1.5 Password Protection Override................................................................................................... 48
4.2 PHOTOMETER PROBLEMS.................................................................................................................... 49
4.2.1 Lamp Low/Filter Low Message is Displayed............................................................................. 49
4.2.2 Filter Wheel Does Not Rotate .................................................................................................... 49
4.2.3 Filter Wheel Runs at an Uncontrolled Rate ............................................................................... 49
4.2.4 Incorrect Control Readings ....................................................................................................... 50
4.2.5 Poor Linearity............................................................................................................................ 50
4.2.6 Erratic Readings ........................................................................................................................ 50
4.2.7 Lamp failure............................................................................................................................... 50
4.2.8 No sampling ............................................................................................................................... 50
5. CALIBRATION ..................................................................................................................................... 51
5.1 CALIBRATION AND ABSORBANCE LINEARITY ..................................................................................... 52
5.2 RESTORE CALIBRATION DATA ............................................................................................................ 52
5.3 TEMPERATURE CALIBRATION ............................................................................................................. 53
5.4 ABSORBANCE CALIBRATION............................................................................................................... 54
5.5 RESTORE FILTER LABELS ................................................................................................................... 55
6. CONTACT INFORMATION ............................................................................................................... 56
APPENDIX 1 – SCHEMATICS................................................................................................................ 57
1. INTRODUCTION
This manual describes service and troubleshooting procedures for 3300 series
instruments. This manual is written with the service technician in mind, and
contains no information for clinical chemistry analysis, or other applications. For
details on instrument operation and specifications, please refer to the Owner's
Manual specific to the instrument.
1.1 Applications
1.1.1 Intended Use
This instrument is intended to be used to read and calculate the results of in-vitro
clinical diagnostic assays, as well as any other application requiring absorbance
or concentration readings at or near the available wavelengths. This general
purpose instrument is intended to be used by laboratory professionals capable of
selecting the appropriate features and options for each specific clinical
application.
1.1.2 Summary of the Instrument

Stat Fax® 3300 is designed for the investigation of Biochemistry Enzyme
Immuno and Drug Levels from human serum, plasma, or urine. A removable
Flowcell installs in the read well to provide extremely rapid fluid sampling with low
carryover. A built-in vacuum pump and an external waste bottle with level
sensing are supplied standard. When the Flowcell is removed, the instrument
accepts standard 12 mm round tubes as well as 1 cm square cuvettes.
The design of the instrument includes many features to minimize operator error,
such as stable factory calibration, automatic zeroing, complete operator
prompting, detailed labeling, pre-programmed calculations, visual and audible
feedback, flags and error messages, and minimal maintenance requirements.
The operating modes are: Absorbance Mode, Standard Mode, Rate Mode,
Factor Mode, Multi-point Mode, and Index Mode.
The basic calculations are permanently stored in memory and include several
single and multi-point equations. Provisions are made in certain cases for
reading specimens in duplicate and/or using the mean reading in calculations.
Each calculation mode is described in detail in the Operator’s Manual. These
modes are keyboard selectable and self-prompting to reduce error and simplify
operation.
System Control
The instrument is based on the 8 bit Z180 microprocessor. The software is
permanently stored in a M27C1001 EPROM. A battery-backed non-volatile RAM
(random access memory) chip (MK48T35) incorporating a real-time clock, (2)
24LC515 EEPROM chips are used to store data such as calibration, test setups,
samples, contains 32k of general use RAM, and also maintains the date and
time. All digital I/O is accomplished with (2) 8255 Programmable Peripheral
Interface. Time intervals and pulse widths are measured using the counter
channels of an 8254 programmable timer.
Awareness Technology Inc. Stat Fax® 3300 Service Manual Rev G 1

User-programmable Memory
User-programmable memory allows the operator to store and name tests in a
numbered test menu. Thereafter, these tests may be readily recalled for later use
with minimal set up. Standard curves are also stored in this memory. Test
protocols remain stored until either changed or deleted by the user. Instructions
for using this memory are given in the Users Test Menu in the Operator’s
Manual.
1.2 Specifications
Specification Date......................................21 September, 2002
Model Name ..............................................Stat Fax® 3300
Spectrophotometer Type ...........................Filter photometer
Optical Configuration .................................Single beam with continuously rotating
filter wheel
Monochromatic or bichromatic reading
8 filter positions
Usable Spectral Range..............................330 to 770 nm
System Procedures ...................................Open and by stored menu
Calculating Modes ....................................Absorbance
Single Standard
Differential samples
Factor Mode
Differential samples
Multi Standard Mode (up to 7
standards)
Multi Standard % Abs (up to 7
standards)
Kinetic Mode (consecutively, or
simultaneously (Batch))
By Factor or by Standard
Fixed Time Kinetic
By Factor or by Standard
Index Mode
Channels ...................................................120 Open
Source of Radiation ...................................Tungsten Halogen, 10 Watt, with
automatic lamp saver
Selection of Wavelength ............................By filter
Filter Type..................................................4-cavity interference, long-life ion
beam-assisted deposition
Wavelength Accuracy ................................+/- 3 nm
Filter Location ............................................After sample (heat absorbing filter
before sample)
Filter Selection ...........................................Automatic by software or via keyboard
Wavelengths ..............................................340, 405, 505, 545, 580, 630 nm
supplied standard
other/additional filters optional
Half Bandwidth...........................................< 10 nm
1/100 Bandwidth ........................................14 nm at 340 nm
False Radiant Energy Ratio.......................< 0.001 at 340 and 405 nm
Cuvette ................................................... 1 cm square, 12 cm round, flow through
Supplied Type............................................Flow-through
Material......................................................316 stainless, borosilicate windows
2 Stat Fax® 3300 Service Manual Rev G Awareness Technology Inc.

Geometry ...................................................Cylindrical, 2.3 mm dia x 5 mm +/-
0.05 mm
Illuminated Volume ....................................21 µl
Minimum Read Volume .............................250 µl
Aspiration/Purge ........................................Vacuum pump at 18 cm of Hg
Valve..........................................................Silicone pinch type
Cuvette Holder...........................................Thermostatically controlled
compartment at 37° C
Detector .....................................................Gallium-Arsenide-Phosphide
photodiode
Signal Processing and Display
Display Type ..............................................240x128 Graphic LCD w/ Backlight
Scale of Display
Absorbance ...............................................-0.5 to 3.5 (flow-through mode)
-0.5 to 2.5 (tube or 1 cm cuvette)
Concentration ............................................Maximum 999,999
Kinetic Results ...........................................Abs/min with resolution of 0.0002
A/min
Zero Compensation ...................................Automatic
Range ........................................................-0.5 to 2.5 absorbance
Signal Outputs
Parallel ......................................................Centronics/IBM-PC compatible
Serial .........................................................RS-232 at 9600 baud, 8 data, 1 stop,
no parity Bi-Directional
Data Input.................................................1) 20 Key Keypad, 2) PS2 101
Keyboard (connector available at back
of instrument)
Spectrophotometric Inaccuracy
Flow-through..............................................< 0.5 % at 1 absorbance, 340/630 nm
NADH solution
< 1% at 2 absorbance, 340/630 nm
NADH solution
< 3 % at 3 absorbance, 340/630 nm
NADH solution
< 0.5 % at 1 absorbance, 405/630 nm
PNP solution
PNP solution
PNP solution
Stability ......................................................Better than 0.003 A/hr monochromatic
after warm-up
Better than 0.001 A/hr bichromatic
after warm-up
Warm-up Time ...........................................90 seconds photometric
15 minutes for temperature
compartment
Electronics .................................................Z180 Microprocessor 18 MHz
128k EEPROM
32 K bytes Nonvolatile RAM (NVRAM)
Power ........................................................Auto-Switching Power Supply
Voltage source: 90 – 264 VAC
Frequency: 50/60 Hz

Power consumption: 60 watts
Installation Category: CAT II
Fuses: 2.5A/250V Fast 5-20 mm Glass Fuse,
2.0A/250V Fast 5-20 mm Ceramic Fuse, (2)
6/10 250V Slow Blow 3AG Fuses
Dimensions and Weight ............................40cm (L) x 37 cm (W) x 14 cm (H) lid
closed (30 cm lid opened), 6.4 kg
Space Requirements .................................10 cm clearance on all sides
Environmental Conditions for Safe Operation: Indoor Use Altitude up to 2000m.
Temperature 5°C to 40°C. (Although it
may be safe to operate in these
conditions, it may not be suitable for
the performance of the user’s tests.
Check with supplier.)
Humidity 85% for temperatures up to
31°C decreasing linearly to 50%
humidity at 40°C. Mains supply
voltage fluctuations not to exceed
±10% of the nominal voltage.
Recommended Operating Temperature ....15-35°C
Recommended Operating Humidity Between 10 and 85%, non-condensing
Design and Instrument specifications are subject to change without notice.

1.3 Warning Markings
1.3.1 Safety Symbols
Safety symbols which may appear on the product:
WARNING Protective Ground CAUTION BIOHAZARD

Risk of Shock (Earth) Terminal Refer To Manual Risk of Infection
FUSE: For continued protection against the risk of fire, replace only with fuse of
the specified type and current ratings. Disconnect equipment from supply before
replacing fuse.
1.3.2 Safety Terms

These terms may appear on the product:
DANGER indicates an injury immediately accessible as you read the

marking.
WARNING indicates an injury hazard not immediately accessible as you read

this marking.
CAUTION indicates a hazard to property including the product.
Terms which may appear in this manual:
WARNING: Warning statements identify conditions or practices that could

result in injury or loss of life. WARNING indicates an injury hazard not
immediately accessible as you read the marking.
CAUTION: Caution statements identify conditions or practices that could

result in damage to this product or other property.
BIOHAZARD: Biohazards are biological agents that can cause disease in

humans. Lab workers handling potentially infectious materials must use
universal precautions to reduce the risk of exposure to these agents.
1.4 Safety Precautions

To assure operator safety and prolong the life of your instrument, carefully follow
all instructions outlined below.
Read Instructions
Review the following safety precautions to avoid injury and prevent damage to
this instrument or any products connected to it. To avoid potential hazards, use
this instrument only as specified. For best results, familiarize yourself with the
instrument and its capabilities before attempting any clinical diagnostic tests.
Refer any questions to your instrument service provider.
Personal Protective Equipment

Many diagnostic assays utilize materials which are potentially biohazardous.
Always wear protective apparel and eye protection while using this instrument.

Follow Operating Instructions
Do not use the instrument in a manner not specified by the manual or the
protection provided by the instrument may be impaired.
Use Proper Power Cord

Use only the power cord specified for this product and certified for the country of
use.
Ground the Product

This product is grounded through the grounding conductor of the power cord. To
avoid electric shock, the grounding conductor must be connected to earth
ground. An alternate method is to attach a ground strap from the external
grounding terminal on the rear panel of the instrument to a suitable ground such
as to a grounded pipe or some metal surface to earth ground.
Observe All Terminal Ratings

To avoid fire or shock hazard, observe all ratings and markings on the
instrument. Consult this manual for further ratings information before making
connections to the instrument.
Install as Directed
The Stat Fax® 3300 should be installed on a sturdy, level surface capable of
supporting the instrument’s weight (25 lbs, 11.4 kg) safely for safety and
ventilation purposes. The mounting surface should be free of vibrations.
Provide Proper Ventilation

Refer to the installation instructions for details on installing the product so it has
proper ventilation. The instrument should be surrounded by the following
clearances: 3 inches (8 cm) around perimeter of unit and 3 inches (8 cm) on top.
Do Not Operate Without Covers

Do not operate this instrument with covers and panels removed.
Use Proper Fuse. Use only the fuse type and rating specified by the
manufacturer for this instrument. Use of a fuse with an improper rating may pose
a fire hazard. Refer to the Power Requirements section for details on fuse
replacement.
Avoid Exposed Circuitry

Do not touch exposed connections and components when power is present.
Avoid Excessive Dust

Do not operate in an area with excessive dust.
Do Not Operate With Suspected Failures

If you suspect there is damage to this instrument, have it inspected by a qualified
service person.
Do Not Operate in Wet/Damp Conditions.
Do Not Operate In An Explosive Atmosphere.

Keep Instrument Surfaces Clean and Dry
Solvents such as acetone or thinner will damage the instrument. Do not use
solvents to clean the unit. Avoid abrasive cleaners; the display overlay is liquid-
resistant, but is easily scratched. The exterior of the instrument may be cleaned
with a soft cloth using plain water. If needed, a mild all-purpose or nonabrasive
cleaner may be used. A 10% solution of chlorine bleach (5.25% Sodium
Hypochlorite) or 70% isopropyl alcohol may be used as a disinfectant. Take
special care not to spill liquid inside the instrument.
Operating Precautions
Be sure to run a sufficient number of controls in each assay. If controls are not
within their acceptable limits, disregard test results.
Biohazard Precautions
BIOHAZARD
WARNING: If any materials are overturned during operation, immediately

set the power switch to OFF (0). This material should be treated as
potentially biohazardous. Appropriate cleanup and disposal of
biohazardous waste should be used.

2. PRINCIPLES OF OPERATION
This section of the manual describes the operation and the interaction of the
plate reader's main systems. Most systems are controlled by the Z180 on the
main circuit board.
2.1 Power Requirements

The Stat Fax® 3300 contains an auto-switching power supply.
Voltage source: .........................................90 – 264

VAC Frequency: .......................................50/60 Hz
Power consumption: ..................................60 watts
Installation Category: .................................CAT II
Fuse: .........................................................2.5A/250 V Slow Blow / 2.0A/250V Slow Blow
A "dead" instrument may result from a blown fuse. However, a blown fuse may
indicate a problem on the main PCB or power supply.
If there is no voltage present at J6, check the fuse located at the back left corner
of the power supply and remove it with a fuse puller, or carefully pry it out with a
small screwdriver. Install a 250V 2A slow blow fuse. Use only the recommended
fuse. Do not substitute other rating! If voltage is present at J6, check for input
voltage on voltage regulators VR2, VR3, and VR4.
For 110-120 V units used in the US, use a UL listed cord set consisting of a minimum 18
AWG, Type SVT or SJT three conductor cord, maximum 3 meters (10 feet) in length,
rated 10 A, 125 V, with a parallel blade, grounding type attachment plug.
For 220-240 V units used inside the US, use a UL listed cord as above, except rated 250
V, with a tandem blade, with grounding type attachment plug. The cord set provided by
the manufacturer meets these requirements.
For other locations, use the power cord certified for the country of use.
Safety Grounding - Do not alter or defeat the safety grounding methods provided. To
avoid the risk of electric shock, the third prong of the AC power plug must be connected
to conductive parts internal to the equipment. Internal fasteners to grounding points are
marked by the IEC 417 symbol 5019. DO NOT loosen or remove these fasteners or
connections. An alternate method of grounding is provided by connecting the grounding
terminal located on the rear panel to a suitable ground.
To avoid electric shock, the power cord protection ground conductor must be connected
to ground.

2.2 Parts and Controls
The following photographs identify major parts and controls of the Stat Fax®
3300
C D
B
A
Figure 2.2-1 Overall view
K
L
I
J
H
G
M
Figure 2.2-2 Rear view

Q
R
T
P S
U
Figure 2.2-3 Waste Bottle Assembly
Reference Figures 2.2-1, 2.2-2, and 2.2-3:
A. Flowcell M. Sensor Jack

B. Waste Bottle (See Detail in Fig. 2.3) N. Vacuum Fitting (Blue)
C. Internal Printer O. Waste Fitting (Black)
D. Graphical LCD Display P. Waste Bottle
E. Key Pad Q. Bottle Cap Assembly (white cap and rubber stopper)
F. Heat Block R. Waste Full Sensor Wires
G. Power Cord Connector S. Waste Tube (Black Fitting)
H. Power Switch T. Exhaust Filter
I. Parallel Port U. Sensor Lead
J. Heat Block Connector V. Vacuum Fitting (Blue)
K. Serial Port W Sample Bar
L. External Keyboard Connector

2.3 General Layout
2.3.1 The Chassis
The chassis consists of two main components:
Cover. The cover is a pressure formed fire-retardant ABS enclosure meeting UL

specification 94V-0. It is attached to the main chassis with 2 #4 self-tap screws.
The display panel is attached to main cover (see Figure 2.2-1). The LCD
display, the interface circuit board, and the keypad are all included in this
assembly.
Base. The base supports the rear electronic deck, and is the foundation for the
plate transport and photometer mechanisms.
2.3.2 Front Panel Electronics

The display: The LCD is driven directly off of the Z180 bus. Address decoding is
done on the main board.
The keypad: The keypad consists of a 5 X 4 membrane keypad. Ports PB0

through PB7 provide the key scan output. The keys are connected as shown in
the drawing. Scanning is performed from PB0 to PB7 outputs and reading from
PC0 to PC3 inputs.
Port Buffer Board: Contains the parallel, serial, and external keyboard ports. A
cable runs from this board to the Main PCB. The ports use an 8255 located on
the Main Board.

2.3.3 Main PCB
Under normal circumstances, there are no adjustments to be made to the main
PCB. Circuit failures are highly unlikely, but if they occur, it is recommended that
the repairs be performed by factory authorized technicians. There are ten test
points on J8 which provide access to a number of vital signals. The instrument
can be observed in operation with an oscilloscope. Typical test point waveforms
are shown in Figure 2.3.4-2.
The current configuration (after Revision Level L artwork) of the Surface Mount
Main PCB is as follows:
Figure 2.3.3.1 – Surface Mount Main PCB Configuration (Rev. L artwork)
The 7-segment LED on the Main PCB was replaced by discrete diodes:
D4, D12, D13, D14, and D17 with Revision level L. Figure 2.3.3-1
displays functions as shown and is further described below:
Diode Label Function

D4 SAMPLE Indicates when the sample sensor is
closed
D6 POWER +15V available on the Main PCB and
processor is active.
D12 VACUUM Indicates a signal to turn on the pump
D13 VALVE A signal to open the pinch valve is
present
D14 CELL HEAT The heating elements in the
photometer cell block are energized.
D17 TUBE IN Detects a test tube or Flowcell
inserted into the photometer.

NOTE: Prior to REV L art work (Surface Mount) this was the configuration
of the Main PCB:
Pump
Tube Detect
Sample Sensor
Valve Action
Cell Heat
Power
Figure 2.3.3-2 - Functions indicated by 7-segment LED
The 7-segment LED on the Main PCB displays functions as shown in

Figure 2.3.3-2 and is further described below:
Pump Indicates a signal to turn on the pump.
Sample Sensor switch Indicates when the sample sensor

switch is closed.
Cell heat The heating elements in the

photometer cell block are energized.
Tube detect A test tube or Flowcell is inserted into

the photometer.
Power +15V available on Main PCB.
Valve action A signal to open the pinch valve is

present.

2.3.4 Photometer Mechanism
The Stat Fax® 3300 uses a single channel silicon diode detector to read the light
signal from a 6-volt gas-filled lens-end tungsten lamp. The continuously rotating
filter wheel contains four fixed wavelength interference filters, and is located
inside the photometer housing underneath the microwell plate. This arrangement
greatly reduces problems due to ambient light and allows for an open frame
structure of the plate transport system.
The photometer is operated under microprocessor control. The filter wheel is

electronically controlled via a TDA7275A speed control chip to four revolutions
per second. Infrared opto switches provide commutation (filter identification)
using the holes in the edge of the filter wheel. One pair provides a count pulse,
and the other provides a home indication. Figure 2.3.4-1 shows the photometer
mechanism. Figure 2.3.4-2 illustrates the photometer and absorbance
conversion signals (filter count, home, sample and hold, filter peaks and
comparator output waveforms).
The photometer mechanism consists of four elements:
• The filter wheel and rotation mechanism: The filter wheel is rotated by
a small DC motor connected to the wheel by a rubber belt. The wheel
speed is electronically controlled to four revolutions per second. As the
wheel turns, the filters mounted in it pass between the light source and
the photo detector, the reading is taken when the filter passes through
the light beam.
• Filter wheel position detection system: Optical gates (beam interrupt

switches) are used to control the filter wheel speed and take readings.
Normally the infrared light from the LCD side of the gate is blocked, but
when the small holes in the filter wheel pass by, the light passes through
and the microprocessor receives a signal. There are two sets of holes.
One set consists of several holes, one at each filter. When one of these
holes is detected, the filter is lined up, and a reading is taken. The other
set is only one hole and it is used primarily for synchronizing the filter
count.
• Light source: The light source consists of the lamp with its built-in lens,
the diffuser, and the aperture block. The light, originating at the lamp,
passes through the diffuser, then through the sample plate, the aperture
block, and then into the photometer. This area under the spot of light is
known as the read site. The connection to the lamp and filter wheel motor
is the junction PCB with an IDC (insulation displacement connector.)
• Optical detection system: This system consists of a photometer PCB

on which is mounted: a light sensitive photodiode and the operation
amplifier. The optical system is connected to the junction PCB using a
14-pin (2 x 7) header cable assembly.

Figure 2.3.4-1 - Photometer Mechanism

Figure 2.3.4-2 - Photometer and absorbance conversion signals
Use the home signal to trigger an oscilloscope to observe the test points J8 as
shown above. Each filter peak is sampled and held (U15 Pin 1) and
simultaneously converted from an analog signal to a digital absorbance by an
exponential capacitor decay circuit (U14 Pin 7) and one of the 16 bit counters in
U16 Pin 11. (See the photometer schematic.) The photometer output is
proportional to the power of the light signal whereas the width of the positive
phase of the pulse at the counter chip is proportional to the absorbance. The
resistance (TP1) across the log cap (simple RC decay) determines the base of
the log (10 for absorbance) and is used to adjust the low end absorbance
calibration (gain). Another potentiometer (TP3) is used to null the offset voltages
in the circuits and serves to adjust the hi-end absorbance indications.
CAUTION: Do not turn trim pots. Refer to the Calibration Section.

2.3.5 Flowcell
If proper care is taken to clean the Flowcell between uses, especially after highly
proteinaceous liquids have been sampled, the Flowcell itself should require little
or no maintenance. The Flowcell should be cleaned regularly as described in the
section entitled Cleaning the Flowcell. If the Flowcell tubing has become
damaged or the Flowcell is clogged, reference the service procedure Flowcell
Tubing Replacement.
Erratic readings (excessive dither) may be a result of trapped air in the Flowcell.
This can be caused by improper installation of, or failure of the Flowcell tubing or
coupling tubes. Refer to the Flowcell Tubing Replacement section. Check the
insertion depth of the Flowcell tubes into the coupling tubes. Ensure that a leak-
free seal is made and that no tubes are kinked or pinched off.
2.3.6 Valve
The valve uses a short length of silicone tubing. If the valve operates but no
sample is drawn up, the pinch valve tubing may be blocked. To check for
clogged valve tubing, unscrew the Flowcell vacuum line from the Luer fitting.
Activate and hold Sample Sensor Optical Switch and listen for aspiration. If you
hear aspiration, the valve is operating, but the Flowcell is clogged. If you do not
hear aspiration, the valve tubing is clogged. Refer to the Valve Tubing
Replacement section.
2.3.7 Vacuum Pump and Pump Control Circuit

The vacuum pump and pump control Circuit should require no maintenance. If
the vacuum pump runs continuously, you probably have a leak in the vacuum
side.
• Check the waste bottle cap and fittings.
• Check that the tubing is firmly seated on barbs and all fittings are tight.
Turn the fittings only until finger-tight; do not over tighten the plastic Luer
fittings.
• If the waste bottle is located at a higher or lower level than the

instrument, such as on a shelf, or if the instrument is used at very high
altitudes, the vacuum trim pot may require adjustment. See the Vacuum
Adjustment section.
• If the vacuum pump is taking longer to achieve full vacuum (runs much
longer than usual, but otherwise works), the exhaust filter is likely
clogged and should be replaced.
In the event that the exhaust filter gets wet due to a waste bottle spill, it
must be replaced. Reference the Exhaust Filter Replacement section for
instructions on replacing the exhaust filter.

2.3.8 Temperature Control
The temperature control system is very stable and ordinarily does not require
adjustment or recalibration. In the event calibration must be verified or re-
established, refer to the section titled Temperature Calibration.
2.4 Parallel Interface

The locations of the parallel port and the RS 232 serial port on the rear deck of
the unit are shown in Figure 2.2-2.
The parallel interface is industry standard Centronics compatible. The cabling is

the industry standard parallel printer cable. Below is the pin out list for the parallel
port on the Stat Fax® 3300:
Pin # Function Direction

1 Strobe To Printer
2 Data0 To Printer
3 Data1 To Printer
4 Data2 To Printer
5 Data3 To Printer
6 Data4 To Printer
7 Data5 To Printer
8 Data6 To Printer
9 Data7 To Printer
11 Busy From Printer
12 Paper Out From Printer
16 Initialize To Printer
19-25 Ground
2.5 Auxiliary and Optional Equipment

2.5.1 Auxiliary Equipment Interface
Parallel Port: The Stat Fax® 3300 can send results out to a printer with a
parallel port. The Stat Fax® 3300 has a 25-pin female connector on the back of
the hatch cover. This connector is a standard parallel port. A standard parallel
cable normally used to connect a PC to a parallel printer can be purchased from
any computer store. See Section 2.4 Parallel Interface for pin outs.
Serial Printer: The Stat Fax® 3300 can print its results out to an Epson
compatible serial printer using the included serial interface port. The data is sent
at 9600 baud, No Parity, 8 data bits, and 1 stop bit. The connection requires a
standard serial cable.
External Heat Block: The Stat Fax® 3300 comes standard with an External
Heat Block. The External Heat Block maintains its temperature at 37.5° C and is
connected to the rear of the Stat Fax® 3300. When active, the temperature is
displayed on screen.
2.5.2 Optional Equipment

External Keyboard. The Stat Fax® 3300 can also be operated using an External
Keyboard attached to the port on the rear panel.

3. SERVICE PROCEDURES
3.1 Opening the Instrument
Refer to Figure 3.1 Instrument Interior. The cover is hinged at the rear panel,
and can be raised to allow access to the inside of the instrument. Disconnect the
power cable, the tubing, and the sensor lead from the rear panel. Locate and
remove the two cover screws from the underside, one on each side (shown with
arrows in Figure 3.1). Gently lift the front of the cover upward, taking care to
clear the photometer. Prop the cover open with a suitable object.
Do not force the cover backwards. Damage to the cover or fittings may result.
To reinstall the cover, reverse the procedure. Carefully lower the cover until it
seats on the chassis, taking care to clear the incubation block and the Flowcell
Luer fitting.

Power
Supply
MAIN PCB
Pump
Valve
Photometer Fan
Cover Screw Cover Screw
Figure 3.1 – Instrument Interior (internal view with cover lifted)

3.2 Cleaning the Flowcell
The Flowcell should be cleaned when the instrument will not be used for an
extended period, e.g. over night, end of shift, and when storing the Flowcell.
Proper cleaning will help to prevent clogging of the Flowcell tubing and valve
tubing. Cleaning is extremely important to obtaining accurate, repeatable results.
If reagent, serum, or other proteinaceous fluid is allowed to dry in the Flowcell, it
is extremely difficult to remove and its presence can affect test results.
To clean the Flowcell:
1. Purge with air for at least 5 seconds.
2. Aspirate several ml of Flowcell cleaning solution. Allow the solution to

remain in the Flowcell for 3 minutes.
3. Aspirate 15 ml of distilled water then purge with air for 5 seconds.
4. Aspirate 0.1N hydrochloric acid (HCl). Allow the solution to remain in the
Flowcell for 3 minutes.
5. Purge with at least 15 ml of deionized water.
6. If the Flowcell is to be removed for storage, purge with air until no more fluid
can be seen flowing into the waste bottle. Otherwise, leave the Flowcell filled
with water.

3.3 Flowcell Configuration
Flowcell Configuration offers two choices; read tubes and cuvettes, or to activate
the Flowcell with options to change the aspirate (sample) volume, and read and
store water reference values.
Flowcell Configuration: Prompt/Response:
From the TOOLS menu (F4) access the Flowcell F4 TOOLS

Configuration screen by pressing:
Numeric Key 2
The screen will display: Current Flowcell STATUS =
Current Flowcell STATUS = ON OFF The current status of the Flowcell will
be highlighted (ON or OFF).
Current Aspiration VOLUME = 500 µL
Press the F1 key to toggle the
Flowcell Mode ON
ON/OFF VOLUME CAL MENU OK
Press the F1 key to toggle the Flowcell Mode ON F1
The screen will display the current aspiration volume. Current Aspiration VOLUME =
To change the volume, press: F2 VOLUME
The display will prompt: Enter Aspiration Volume:
Make a selection from the seven

ASPIRATION VOLUME volume choices.
0 = 750 3 = 450 6 = 300 Press ENTER (F4) to accept.

1 = 600 4 = 400 7 = 250
2 = 500 5 = 350 Press CLEAR (F3) to entry.
Press QUIT (F1) to return to the Main

Enter Aspiration Volume: Menu.
QUIT CLEAR ENTER
NOTE: When reading sample in Flowcell Mode, remember to have more sample than the
aspiration volume selected. Attempting to aspirate the exact amount of sample may result in poor
results due to air mixing with sample. User technique, the shape of the bottom of vessel being
used, and the location of the tube during sampling can affect this process.

Flowcell Configuration: (Continued) Prompt/Response:
Select the CAL MENU by pressing F3 F3 CAL MENU
The screen will display: FLOWCELL CALIBRATION
FLOWCELL CALIBRATION
Enter Menu Number:
1 = Read Water Reference
2 = Flowcell Alignment A short description of each option
follows below.
Select the numeric key that

Enter Menu Number: corresponds with the option desired.
QUIT
NOTE: These options may only be used if the Flowcell is

active.
Press:
Numeric Key 1 Read Water Reference:
The instrument references water instead of air when reading with the Flowcell. These
reference values are stored in non-volatile memory and used whenever the Flowcell is
active and until new values are read. This should be done before Flowcell Alignment.
In order to insure good blanks when using the Flowcell, conduct Read Water Reference on
a regular basis; time intervals depend upon instrument usage and when encountering
unexpected (very high, very low) blank values.
Numeric Key 2 Flowcell Alignment:
NOTE: This option is not necessary under normal operating conditions.
Necessary when a new Flowcell is used, or if a lamp replacement has occurred. Conduct
Read Water Reference before and after Flowcell Alignment.
The Flowcell should be active but NOT inserted into the read well.
– Press F3 PRINT RESULT to print the current reading.
– Insert the Flowcell into the read well.
– Submerge the end of the tubing into a container of water and activate the Sample
Bar. Continue with the interruption of the beam until water starts to flow into the
Waste Bottle. This will decrease the likelihood of air bubbles interfering with an
accurate reading.

Flowcell Configuration: (Continued) Prompt/Response:
Numeric Key 2 Flowcell Alignment: (Continued)
– This reading should be slightly over half of the original reading. If it is not, the
Flowcell must be adjusted.
– If the displayed value is less than 50% of the reading with the Flowcell removed,
purge and remove the Flowcell.
– Adjust the set screw with the hex wrench supplied. Turn the set screw 1/4 turn in
either direction and replace the Flowcell.
– Sample water again. If the value increases, turn the set screw in the same
direction. If the value decreases, turn the setscrew in the opposite direction.
– Repeat until the value is >50%.
– When complete, press QUIT to return to the main prompt. New water values must
be read as described above in Read Water Reference.
• Function Key F1 QUIT to return to Main Menu
3.4 Flowcell Alignment

Note that Flowcell alignment is not necessary under normal operating conditions.
However, it is necessary to perform Flowcell alignment when a new Flowcell is

used, or if a lamp replacement has occurred. Conduct Read Water Reference
before and after Flowcell Alignment.
The Flowcell alignment instructions can be found above in Section 3.3 Flowcell
Configuration.

3.5 Flowcell Tubing Replacement
The Flowcell utilizes 1.2mm I.D. Teflon tubing for the Inlet (sample) and Exit tubes.
Replacement tubing is included with the tubing kit. Follow this procedure to replace the
Flowcell tubing.
Materials/tools required:
◦ Tubing kit ◦ Phillips screwdriver
◦ Tweezers ◦ Isopropyl alcohol
Inlet Exit tube

(sample)
tube
Figure 3.5A Flowcell
• Lift and remove the Flowcell out of the read well.
Flowcell handle
Flowcell upper body
Flowcell lower body

assembly
Figure 3.5B Flowcell

• Remove the two flathead screws from the retainer plate to remove the Flowcell
handle (reference Figure 3.5B and 3.5C)
Flowcell handle
Remove the
retainer plate
from the
Flowcell
handle by
removing the
two flathead
screws.
Retainer plate
Figure 3.5C Remove the flathead screws on the retainer plate
Inlet tubing Exit tubing
Tube support Flowcell handle
Figure 3.5D Remove Exit tubing
• Remove the Exit tubing (reference Figure 3.5D) at the metal tube and pull
it through the Flowcell handle. Slip the Inlet tubing out and away from the
Flowcell handle.
• Remove the two pan head screws with split lock washers from the underside
of the retainer plate (reference Figure 3.5E) and slide off the retainer plate.

Remove the
two pan head
screws with
split lock
washers from
the underside
of the
retainer plate.
Figure 3.5E Remove two pan head screws with split lock washers
• Remove the upper cell body from the lower cell body assembly by removing
the two flat head machine screws from both sides of the upper cell body (see
Figure 3.5F). Once the screws are removed, pull the upper cell body apart
from the lower cell body assembly.
Upper cell body

Remove screws
from both sides
Lower cell body
assembly
Figure 3.5F Remove the lower cell body assembly

• Remove the four flathead screws from the cell retainer plate (reference
Figure 3.5G).
Remove the
four flathead
screws from
the lower cell
body retainer
plate.
Figure 3.5G Lower cell body assembly
• Once the cell retainer plate is removed, lift off the cell window and the seal to
access the metal cell insert. Grasp the cell insert and lift it out from the lower cell
body.
• Remove the Inlet (sample) tubing from the steel tube on the cell insert. Remove
the ground contact sleeve from the metal tube.
• Locate the Inlet (sample) tubing from the tubing kit. Carefully press the end of
the Inlet tubing (the swaged end) onto the short steel tube of the cell insert.
• Reassemble the lower body assembly following the illustration shown in

Figure 3.5H.
Read the tip that follows for immobilizing the glass cell window to make
reassembly easier.

Useful T I P for reassembling the
lower cell body assembly:
¾ Ensure that an o-ring is inserted into the cell retainer plate (reference
Figure 3.5H).
¾ Position an o-ring, a glass cell window, a Teflon seal, the cell

insert, and the remaining Teflon seal into the lower cell body per
Figure 3.5H.
¾ While holding the remaining glass cell window by its edge with
tweezers, dip the remaining glass cell window in isopropyl alcohol (this
will help immobilize the cell window for ease in placement) and place it
in position on the underside of the cell retainer plate.
¾ Insert two of the screws into the cell retainer plate at opposing corners
and set the cell retainer plate into position and tighten the screws.
¾ Install the remaining two screws.
• Once the lower cell body is assembled, attach the upper cell body to the lower
cell body assembly by slipping the Inlet tubing and metal cell insert through the
center hole of the upper cell body. Push the two pieces together until they are
flush. Reinstall the two flathead machine screws to each side of the upper cell
body to hold the upper cell body and the lower body cell assembly together
(reference Figure 3.5F and Figure 3.5I).
Insert flat head

screw on both
sides and tighten
Figure 3.5I Upper cell body and lower cell body assembly
• Slide the Inlet tubing and metal insert through the center hole on the retainer
plate (reference Figure 3.5E).

Figure 3.5H illustrates the process of assembling the Flowcell lower body once it has been
taken apart for inlet (sample) tube replacement or for cell insert replacement.
Figure 3.5H Flowcell lower body assembly

• Reinstall the two pan head screws with split lock washers to attach the
retainer plate to the assembly (Figure 3.5J).
• Insert the ground contact sleeve over the metal cell insert tube (reference
Figure 3.5J) pressing it into place until the ground contact sleeve is at the
height of about 1/8”.
Underside of
retainer plate
with the two
pan head
screws with
split lock
washers in
place
Metal cell
insert for exit
tube with
ground contact
sleeve in place
Figure 3.5J Flowcell lower body assembly
• Feed the Exit tube through the hole provided in the Flowcell handle (reference
Figure 3.5D).
• Feed the Inlet tube into the Inlet (sample) tube support via the hole provided
inside the Flowcell handle.
• Attach the Exit tubing to the metal cell insert (reference Figure 3.5D).
• Carefully, without kinking the tubing, snug the Exit tubing and the Inlet tubing into
place such that the retainer plate can be fastened to the Flowcell handle.
CAUTION: Handle carefully. Do not pinch the tubing.
• Reinstall the two flathead machine screws (reference Figure 3.5C) to hold the
retainer plate and assembly to the Flowcell handle.
• Follow the process for testing for leaks in the Flowcell assembly.

3.6 Testing for Leaks in the Flowcell Assembly
Test for leaks when the Flowcell has been disassembled and reassembled as for
tubing or cell insert replacement. The Flowcell should be active but NOT inserted
into the read well until stated to do so.
Testing for Leaks in the Flowcell Assembly: Prompt/Response:
From the TOOLS menu (F4) access the Flowcell F4 TOOLS

Configuration screen by pressing numeric key 2
Numeric key 2 Flowcell
Configuration
The screen will display: Current Flowcell STATUS =
The current status of the Flowcell will

Current Flowcell STATUS = ON OFF
be highlighted (ON or OFF).
Current Aspiration VOLUME = 500 µL
Press the F1 key to toggle the
Flowcell Mode ON
ON/OFF VOLUME CAL MENU OK
Press the F1 key to toggle the Flowcell Mode ON F1
Select the CAL MENU by pressing F3 F3 CAL MENU
The screen will display: FLOWCELL CALIBRATION
FLOWCELL CALIBRATION
Enter Menu Number: Numeric key 2
1 = Read Water Reference
2 = Flowcell Alignment Flowcell Alignment
Enter Menu Number:
QUIT
Select Flowcell Alignment by pressing numeric key 2
NOTE: These options may only be used if the

Flowcell is active.

Testing for Leaks in the Flowcell Assembly: Prompt/Response:
Perform an air voltage reading (the Flowcell should be F3 PRINT RESULTS

active but NOT inserted into the read well) by pressing
the Sample Bar. ENTER
Press F3 to print the result and press Enter.
Press LINE FEED twice to advance the paper feed so LINE FEED
that the reading is visible.
LINE FEED
Install the Flowcell into the photometer. Snug the Exit
tubing into the Flowcell Luer fitting (do not over tighten).
Fill the Flowcell with water by placing the Inlet (sample)

tube into a container of deionized water.
Press and hold in the Sample Bar until there are no air
bubbles visible in the Exit tube feeding into the Waste
bottle.
This reading displayed should be slightly over half of the

original reading. If it is not, see the following scenarios
to correct the problem:
1) Check to see that the Inlet (sample) tubing is

not kinked behind the retainer plate in the
Flowcell handle. Remove the two flathead
screws from the retainer plate under the
Flowcell handle. Ensure that the tubing is not
kinked as this will hinder its ability to aspirate.
Reinstall the retainer plate to the Flowcell
handle. Perform the reading again. If the
reading is not > 50% of the original reading
see the step below.
2) Disassemble the Flowcell as detailed in

Section 3.5 Flowcell Tubing Replacement.
Ensure that the o-rings, glass cell windows,
and seals are seated properly. Ensure that
the swaged end of the Inlet (sample) tubing
is securely attached to short steel tube of the
cell insert. Reassemble the Flowcell and
perform the reading again.
When complete, press QUIT to return to the main

menu.

3.7 Flowcell Cell Insert Replacement
The Flowcell may be disassembled for extensive cleaning or replacement of the
cell insert.
To replace the cell insert, follow the instructions on disassembling the Flowcell in
Section 3.5 Flowcell Tubing Replacement. Once the lower cell body assembly is
disassembled, remove the cell insert and install the new cell insert. Assure the
correct orientation is used when installing the new cell insert by following the
illustration in Figure 3.5H. Reassemble the Flowcell following the instructions in
Section 3.5 Flowcell Tubing Replacement. Use the procedure provided to check
for possible leaks in the Flowcell.
After assembly, complete the procedure in Section 3.4 Flowcell Alignment to

restore optical alignment.

3.8 Main PCB and/or Photometer Replacement
3.8.1 Photometer Replacement
1. Open the instrument. Refer to Figure 3.1. Locate the photometer. Unplug
cables from the main PCB.
2. NOTE: For ease of relocating for reassembly, mark the chassis with a pencil
along the front and side edges of the photometer subassembly as shown
below.
3. Locate the two #6 screws holding the photometer assembly to the chassis.
From the bottom of the instrument, remove these screws, washer, and the
speed nuts from inside the instrument.

4. Install the replacement photometer subassembly to the chassis by replacing
the two #6 self tap screws with internal tooth lock washers through the
chassis base and the photometer mounting slots and into the speed nuts.
Line up photometer with locating marks made in step 2. Connect the cables
removed in step 1.
NOTE
The internal tooth lock washer must make electrical contact (ground) with
the chassis base for low noise operation.

NOTE:
Screw
locations
shown at
black
circles.
Figure 3.6.2 Main PCB
NOTE: Screw locations shown above with black circles
3.8.2 Main PCB Replacement

1. Open the instrument. Refer to Figure 3.1. Locate the Main PCB. Unplug
cables from the main PCB and disconnect the wires from the Power Supply.
2. Refer to Figure 3.6.2. Remove the four screws securing the Main PCB.
Secure the replacement PCB in the same fashion as the removed PCB.
3. Reconnect Power Supply Wire Harness. Plug in all remaining cables.

3.9 Valve Tubing Replacement
It is not recommended to replace any tubing while the instrument is performing
properly. However, the short length of silicone tubing used in the sampling valve
may become clogged or worn with age. A replacement is included in the tubing
kit.
Set the power switch to OFF (O). Open the instrument as described in
Section 3.1 Opening the Instrument. Refer to Figure 3.1. Locate the valve.
Refer to Figure 3.9. Pull back the pinch bracket and remove the valve tubing
from the valve body.
Disconnect the valve tubing from the fittings at both ends.
Install the replacement tubing to the valve body in a similar manner.
Push the tubing over the tubing barbs until seated. Be especially careful not to
kink, stretch, or tension the tubing.
Lower the cover and replace the screw.
Pull towards the

front of the
instrument to
free tubing
Figure 3.9 Valve Tubing Replacement

3.10 EPROM Replacement
1. Firmware update consists of one EPROM to be installed in the instrument as
follows: Access the circuit board by turning off the instrument, disconnecting
the power cord and removing the cover as shown in Section 3 Service
Procedures.
2. Locate the EPROM socket (see Figure 3.10.2) and push the small blue
levers outward at both ends of the socket (see Figure 3.10.3). This frees the
EPROM for easy removal and insertion.
3. If necessary, gently bend the pins of the new EPROM so that the pins are
perpendicular to the EPROM (see Figure 3.10.1). Hold the EPROM by the
body and bend the pins against a flat surface such as a table to bend all
the pins the same amount. Do not over bend the pins, since they are easy to
break if bent too far.
Figure 3.10.1 - Correct Alignment of EPROM Pins
4. Install the EPROM with the notch oriented as shown in the label on the
EPROM socket. The notch should face toward the left side of the
instrument. Figures 3.10.2 and 3.10.3 display the location of the EPROM
on the Main PCB and an enhanced view of the EPROM label showing the
direction of the arrow for proper orientation.
5. Reinstall the cover and power cord. Turn the instrument back on.

EPROM Replacement (Continued)
Figure 3.10.2 - Location of EPROM on Main PCB
Note the
direction of
the arrow on
the EPROM
label for
proper
orientation.
Figure 3.10.3 – Enhanced view of the EPROM

3.11 Filter Change Procedure
Refer to Figure 3.11.2 Photometer Illustration for this procedure.
Materials/tools required:
• Replacement filter with neutrals and retainer
• Phillips screw driver
• Flat blade screw driver (small)
• Awl with blunt tip (or similar for prying)
• Wooden dowel (or similar for pressing) 7/16" diameter
1. Disconnect the unit from the AC power source and detach the power cord.
Open the instrument (see Section 3, Service Procedures). Set the cover out
of the way.
2. Locate the photometer. Unplug the 4 pin lamp/motor cable from the
interconnect printed circuit board (PCB). Using the flat blade screw driver,
unplug the gray cable from the interconnect PCB.
NOTE: Do not remove the spacers from the photometer. Removing or

altering their position on the photometer will require the unit be
realigned per the procedure in the service manual. Do not move the
lamp or the lamp bracket.
3. IMPORTANT: Refer to Figure 3.1. Follow the procedure in Section 3.8 Main
PCB and/or Photometer Replacement for removing the photometer.
4. Remove the four 6-32 screws retaining the photometer cover. Set the cover
aside. Remove the two 4-40 screws securing the photometer PCB. Remove
the drive belt from the filter wheel. Using the blade screwdriver, remove the
filter wheel shaft. Remove the wheel. Remove the shaft taking care not to
misplace the nylon spacer/washers.
Figure 3.11.1 - Filter Wheel

5. Locate the filter to be removed (see Figure 3.11.1).
6. There are two means of filter retention in field use; silicone glue or a retaining
ring. If a retaining ring is used, carefully pry two to three of the retaining ring
teeth away from the filter until the ring is dislodged and remove the ring and
filter. If silicone glue is present, remove the silicone and push the filter from
the wheel using the eraser end of a pencil or other soft blunt object and any
residual glue should release. Remove any neutral or screen disks that may
remain in the filter cavity.
7. Locate the neutral filters and/or screens that were included with the
replacement filter and drop them first into the cavity. Next insert the filter with
the mirror side down. Place the replacement retainer over the filter so that the
tabs are angled away from the filter and press in place with a 7/16 diameter
wooden dowel or similar object.
8. Reinstall the filter wheel on the bracket taking care not to pinch any washers
under the shoulder of the shaft. Tighten the shaft. Replace the belt. Replace
the photometer PCB taking care to center the board around the shaft.
Tighten the two 4-40 screws. Replace the optical cover taking care to
position the gray cable in the slot of the cover. Do not pinch the cable
under the cover. Insert the three 6-32 screws and snug up. Do not over
tighten.
9. Reinstall the photometer assembly as per Section 3.8.
10. If necessary refer to the lamp alignment procedure in the service manual.
11. Plug the cover cable back into the main PCB. Replace the cover but do not
install the cover screws. If an updated EPROM was included with the filter
replacement kit, install it now referring to the EPROM replacement sheet.
12. Attach the power cord and connect the unit to the AC power source. Turn the
unit on. The displayed numbers represent the voltages of the installed filters.
All voltages should be between 2 and 10 volts. To terminate this mode, press
Clear as required.
NOTE: If the filter voltage is not in the range specified in the previous step,
check lamp alignment. If the lamp alignment is properly set, the neutral
density filters may need to be changed. Add neutrals to lower the voltage.
Remove neutrals to increase the voltage. Never adjust the trim pots on the
main PCB to obtain the correct voltage. Refer to Section 4 Troubleshooting
as needed.
13. Detach the unit from the AC power source. Replace the two cover screws. Refer
also to Section 3.12 Lamp Replacement and Section 4 Troubleshooting.

Remove
Remove Connectors
Screws
Red Wire
Remove (3) screws holding photometer to the base.

Belt Filter Wheel Shaft Retainer
Filter
Remove retainer
(if used), filter,
and neutrals with
soft, blunt object.
Two Nylon
Washers
Install replacement
neutrals, filter, and One Nylon
retainer using 7/16 Washer
dowel.
Figure 3.11.2 - Photometer Illustration

3.12 Lamp Replacement
The lamp should be replaced only if it fails to light, or several filter voltages are
reported as low.
To replace the lamp, follow the procedure below.
CAUTION: Turn the power OFF and unplug the instrument

Open the instrument as described in Section 3.1 Opening the Instrument.
Locate the photometer and the lamp at the right side of the photometer. Refer to
Figure 3.12-1 Lamp Replacement. The figure shows the right side view of the
photometer assembly.
CAUTION: Lamp is HOT. Allow the lamp to cool before handling.

Avoid handling with bare skin.
Loosen but do not remove the two center lamp connector screws. Remove the
lamp by lifting upward.
Use a pair of pliers or tweezers to handle the new lamp.
Avoid handling with bare skin. Insert the lamp leads into the connector until they
hit bottom. Refer to Figure 13.12-2, for proper lamp alignment. The lamp filament
must be centered on the lens and the lamp body must be parallel with the lens
bracket. While holding the lamp in alignment, tighten the lamp connector screws.
Set the power switch to ON. Observe the projection of the light from the lamp
onto the cell holder (behind the lens). Refer to Figure 3.12-2 for spot alignment.
The spot should be small and centered on the oval hole in the cell block (behind
the lens). If the spot is not centered, use the adjustment screws to position the
spot. The vertical adjustment screw raises and lowers the lamp bracket. The
lamp bracket is slotted at the horizontal adjustment screw, so that the lamp
bracket can be moved. The horizontal adjustment screw serves to lock down the
lamp bracket.
Insert a borosilicate 12 mm tube filled with plain water into the read well. Do not
use a soda-lime glass tube, since it does not transmit at 340nm. Press F4
(TOOLS). Press 4 and ENTER. Press 1 and Enter. The display shows the
voltages for each filter.

Figure 3.12-1 - Lamp Replacement
X X
Correct Incorrect Incorrect
Figure 3.12-2 – Proper Lamp Alignment

3.13 Vacuum Adjustment
For the purpose of adjusting the vacuum perform the following:
• With the Waste bottle assembly connected properly to the instrument

(reference Section 2.2 Parts and Controls), disconnect the vacuum line at
the blue fitting at the rear of the instrument; connect this vacuum line with
the blue luer to the vacuum gauge; connect the vacuum gauge to the
back of the instrument at the blue fitting forming a tee (Figure 3.13-1).
Vacuum gauge
Pressure side of Instrument rear

waste bottle panel
Figure 3.13-1 Vacuum gauge connection
• Set Flowcell Mode On via the TOOLS menu F4
• Select Flowcell Configuration numeric key 2
• Toggle the Flowcell Status ON via the F1 key
• Select F4 OK
• Press and release the Sample bar. The vacuum gauge should read 7.0 ±
0.5. If not, adjust trim pot TP2 until the reading on the vacuum gauge is
within the acceptable range. When the reading on the vacuum gauge is
within the acceptable range, remove it and connect the vacuum line to
the instrument at the blue fitting.
• Perform Self Check by selecting the TOOLS Menu F4; select Diagnostics
Menu numeric key 4; select Self Check numeric key 6, and check that the
vacuum system reading displayed is OK, select F1 to Quit.
System Diagnostics:
EEPROM OK
NV RAM OK
Vacuum System OK
Aspiration Valve OK
Photometer OK
______________________________________
QUIT REPEAT ENTER

3.14 Exhaust Filter Replacement
To replace the exhaust filter:
• Set the power switch to OFF (O).
• Carefully release pressure from the Waste bottle.
• Before uncoupling the exhaust filter from the Inlet tubing, note the
orientation of the wording (INLET) on the exhaust filter. The side of the
exhaust filter with the wording (INLET) should face away from the blue
vacuum fitting (see Figure 3.14-1).
• Pull the tubing from the fittings on the filter.
• Install the new filter with the INLET side pointing away from the blue vacuum
fitting. Push the tubing on the fitting until seated.
Exhaust
filter
Inlet
wording
Vacuum
fitting (blue)
Figure 3.14-1 Exhaust Filter

4. TROUBLESHOOTING
Presented below are some possible problems and their solutions.
4.1 Keypad Problems

4.1.1 No Keys Function
• If on power up the LCD functions, the interconnection between the main
circuit board and the keyboard interface is OK.
• If the unit beeps briefly immediately at power-up and no keys function,

either the cable is not plugged in correctly, the key tail is shorted, or there
is a stuck key. Unplug the keypad to determine if it is malfunctioning.
• Check that the tracks are covered down to the insertion point. If the
covering has been damaged, insulate it with clear tape.
• If the problem still persists, massage over the keypad until the beeping
starts. If necessary, stick the key with a pin to equalize the air pressure
inside. If necessary, replace the keypad. If the tracers on the key tails
are missing conductive material at the end that plugs into the interface
PCB, the insulation may be trimmed back 1mm at a time to expose fresh
contact material.
4.1.2 A Row or Column of the Keypad Does Not Function

• Check that the key tails are properly inserted and covered to 1/4" from
the end as discussed in Section 4.1.1. If a row (horizontal) does not
function, check that the low level on the appropriate line of Port B can be
transmitted to the desired line of Port C. Reference schematic. It is highly
unlikely that a component has failed. It is more likely that a plated through
hole on the interface board has failed. Trace the circuit on the board, and
replace if necessary.
4.1.3 The Keyboard and Display Do Not Function

• The problem is probably the connection between the keyboard interface
and the main board. Make sure that the 14 pin cable is plugged in
correctly on both ends.
4.1.4 Keypad Key Stuck

• A keypad with a stuck key will be ignored by the firmware so that an
external keyboard can be used. A series of thirty beeps will be emitted,
and the keypad will be disabled.
4.1.5 Password Protection Override

• To override the password protection, power-up the instrument while
pressing the numeric 5 key on the internal keyboard for a short duration,
such as five beeps.
NOTE: To avoid the “stuck key” scenario, it is Important that the key be
pressed for a very short duration, such as five beeps maximum.
• The password control window will display the status of the password; the
status can be changed from ON to OFF.

4.2 Photometer Problems
4.2.1 Lamp Low/Filter Low Message is Displayed
• Check that the lamp is illuminated and is properly aligned.
• Insert a borosilicate 12 mm tube filled with plain water into the read well.
Do not use a soda-lime glass tube, since it does not transmit at 340nm.
• Press F4 (TOOLS).
• Press 4 and ENTER.
• Press 1 and Enter.
• The display shows the voltages for each filter. Make sure that all are
greater than 2 volts. If a particular channel is low, the filter may be
degrading. Ensure that the lamp is fine and that there is nothing blocking
the light path such as dirt or dust. Replace if less than 1 volt.
4.2.2 Filter Wheel Does Not Rotate

• Check that the wire harness that goes from the main circuit board to the
photometer is installed properly.
• If the filter wheel motor is running, but the filter wheel does not seem to
be rotating, check the two rubber belts.
• If the motor is not turning, check that the motor has between +5 and +6
volts across the motor terminals. (Make sure that the reader is attempting
to read a plate or running test 186.)
• If there is no voltage on the motor, check for a high level on ULN2003,

pins 1 and 2. Also check that the 7806 has +6 volts on its output
terminal.
4.2.3 Filter Wheel Runs at an Uncontrolled Rate

• Check that the wheel count pulse is transmitting to the NMI pin of the
Z180. It should be a narrow, negative spike.
• Also check that the wheel home pulse is arriving at the 8255 port, PC6
pin 11.
• If neither are present and the cables are installed correctly, check the IR
LCD pair on top of the photometer housing for 1.2 to 1.5 volts between
the black and the white wire. A reading of +5 volts here indicates an open
LCD or unterminated return.

4.2.4 Incorrect Control Readings
• Check that the procedures and materials used were valid. Turbid or
contaminated reagents may affect absorbance readings.
• Reading reference dyes can be very helpful in separating instrument problems

from reagent problems.
• Be sure that the appropriate wavelengths were selected for the chromophore
being read.
• Tubes should have no bubbles, condensation, scratches or smudges.
4.2.5 Poor Linearity

• If the instrument is several years old, or has been operated in very humid
conditions, new optical filters may be needed.
The instrument incorporates interference filters of an advanced technology, and

will provide extended life in humid environments when compared to standard soft
interference filters.
However, excessive humidity should be avoided. The instrument will require

service to replace the filters.
4.2.6 Erratic Readings

• One possible source of erratic readings (excessive dither) is trapped air in the
Flowcell. This can be caused by improper installation of the Flowcell tubing.
Refer to the Flowcell Tubing Replacement section. Check the insertion depth of
the Flowcell tubes. Ensure that a leak-free seal is made.
4.2.7 Lamp failure

• The lamp is rated to read over 300,000 tubes, and the lamp saver feature
minimizes lamp idle time. Lamp replacement is only indicated when the lamp fails
to light, or when the message “Lamp output low!” is displayed. Press LAMP to
turn the lamp on or off. If the lamp fails to light, refer to the section titled Lamp
Replacement.
4.2.8 No sampling
• If you can hear the valve cycle, but no sample is drawn up, the valve tubing may
be blocked. Press and hold PURGE several times. Disconnect the Luer fitting at
the rear of the Flowcell. Press PURGE and listen for aspiration. If air is heard
entering the fitting, the valve tubing is clear, but the Flowcell is blocked. Refer to
the Flowcell Cleaning section and the Flowcell Tubing Replacement section.
• If the valve clicks but the pump does not run when you activate the
Sample Switch, the valve tubing may be stuck closed. If this happens,
remove the front cover screw and lift open the cover. Pull the pinch
bracket against the spring tension (to open the valve manually). Gently
pull the tubing slightly to break the seal. See the section titled Valve
Tubing Replacement for a diagram and more information about the valve
tubing.

5. CALIBRATION
This instrument relies on software calibration. All calibration data is stored in
serial EEPROM memory. This data is used by the microprocessor to
compensate for variations in electronic components and manufacturing. During
factory calibration, the calibration data is set and any hardware adjustments are
made using precision reference standards. The calibration data is then recorded
on a label located on the bottom of the instrument. The factory calibration is very
stable and does not drift appreciably over time, making trim pot or other
component adjustments unnecessary unless the trim pots are disturbed or
calibration-related components replaced.
Recalibration of the instrument, software or otherwise, should not be considered

until all possible interfering factors have been ruled out. If field calibration is in
fact necessary, it should only be performed with the proper reference materials
and instruments. In any case, software calibration should be attempted before
any trim pot or component replacements or adjustments are considered.
The temperature calibration data is stored as offsets which the instrument adds
to the sensed temperatures. The absorbance calibration data is stored as a
scaling factor, which is the ratio of a known reference absorbance to the
instrument's reported absorbance. The calibration data may be printed at any
time by selecting test #213. This prints the serial number of the instrument, the
date of last calibration, and the stored calibration data for the absorbance and
cell temperature.
The calibration data is stored with a check sum that is recalculated and
compared each time a mode is selected. Failure to recover the calibration data
properly (checksum failure) will be indicated on the display and the internal
printer:
Do Temperature Calibration Test
Do ABS Calibration Test
The instrument annunciates this condition with multiple beeps. The temperature
offset for the read well is then set to 0.0 and the absorbance scaling factor is set
to 1.000. In this condition, the maximum temperature error is ±1.5 ½C and the
maximum absorbance error is ±10%. The calibration data can be restored as
described in “Restore calibration”.
The hardware calibration of the instrument in time is based on an 18 MHz ceramic

resonator RES1. Calibration in temperature is based on trim pot TP4 and time.
Calibration of absorbance is based on C17, a 0.1µf polystyrene capacitor, trim pots TP1
and TP3, and time.

5.1 Calibration and Absorbance Linearity
Each instrument is calibrated during manufacturing using standards that are
traceable to the National Institute for Standards and Testing (NIST), and is tested
to verify its linearity to 2A. This preset calibration is very stable. Absolute
calibration can be verified with the use of NIST filters, or by periodic comparison
to a reference instrument that is known to be calibrated to NIST filters.
Calibration may be confirmed using a commercially available calibration check
set which can be obtained from your distributor. A periodic verification of
instrument linearity is advised. Since most lab test results are based upon
standards rather than upon absolute absorbances, the linearity of the instrument
is the more critical indicator of instrument performance.
CAUTION: DO NOT ALTER ANY POTENTIOMETER SETTINGS
Changing these settings will make the factory calibration data invalid.
In the unlikely event the calibration data is lost or corrupted, the

absorbance factor is set to 1.000 and the temperature offset adjustments
for the cell are set to 0.0.
Do not enter values other than those recorded on the calibration label
unless absolutely necessary.
5.2 Restore Calibration Data

Each unit is electronically calibrated at the factory. The calibration values are
entered by the keyboard and stored in nonvolatile memory. The instrument will
not accept a change greater than +/- 10% (.900-1.100) for the absorbance factor,
nor will it accept a temperature offset change greater than +/- 2.5°C. Only
minimal calibration adjustments are accepted from the keyboard.
Do Temperature Calibration Test
Do ABS Calibration Test
If either of these messages are printed or displayed, it indicates that the

calibration values have been lost. These messages will be printed each time that
the instrument is turned on, a mode is selected, or a test is recalled. The
instrument will continue to operate, but the calibration must be restored to ensure
the accuracy of the instrument.
WARNING: DO NOT ALTER ANY POTENTIOMETER SETTINGS !
Changing these settings will make the factory calibration data invalid.
In the unlikely event the calibration data is lost or corrupted, the

absorbance factor is set to 1.000 and the temperature offset adjustments
for the cell are set to 0.0.
Do not enter values other than those recorded on the calibration label
unless absolutely necessary.

Follow these steps to restore the electronic calibration:
1. Shut off the instrument. Remove any tubes or cuvettes from the read well.
Carefully lift up the instrument and locate the Calibration Data label on the
underside of the unit. There are (2) values recorded there: Absorbance and
Cell Temp. Write down these numbers.
2. Set the power switch to ON(1).
3. If the date and time have been reset or are incorrect, enter the correct date
and time. See the section “Set Date and Time” in the Operator’s Manual.
4. Press TOOLS (F4). Press 4 and Enter. The Diagnostics Menu is shown.
Choose 3 for Absorbance Calibration and Enter. Enter the Number.
5. Choose 4 for Temp Calibration and Enter. Enter the Number.
6. Press Run Test (F1), type in 213, and press ENTER to get a report of the
calibration data. The cell temperature adjustment will be printed along with
the absorbance adjustment. Make sure that these values are the same as
those recorded on the calibration label.
5.3 Temperature Calibration

Materials and equipment necessary:
• 12 mm borosilicate tube
• Reference Thermistor probe
• YSI Inc. #44108 or equivalent.
• 30K @ 25°C, ± 0.2° interchangeability
• Stat Fax® 3300 Temperature Calibration Fixture
• Digital ohmmeter (DMM), 0.5% or better accuracy
Temperature calibration:
1. Ensure that the Flowcell is active.
2. Remove the Flowcell and install the Temperature Calibration Fixture into the
read well.
3. Allow 15 minutes for equilibration.
4. Set the DMM to read 20K. Read the resistance of the Thermistor and the
fixture. If the resistance is between 17724 ý and 18712 ý, no adjustments
are needed. Otherwise, continue to the next step.

Temperature calibration: (Continued)
5. Calculate the temperature offsets using the following formula:
T = R - 18210
Where:
R is the measured resistance.
C is equal to 750 if the measured resistance is greater than 18210.
C is equal to 720 if the measured resistance is less than 18210.
6. The display shows:
Cell adjust=
Type the offset calculated for the cell and press ENTER. The maximum
allowed adjustment is ±3.0C . Entering values that exceed this net number
will produce an out of range message.
7. Repeat steps 4 through 8 until the temperature is 37°C ± 0.3.
8. Select test #213 to see the current net adjustments. Record the new values
on the calibration label located on the bottom of the instrument.
5.4 Absorbance Calibration

Materials and equipment:
• 12 mm borosilicate tube
• Reference spectrophotometer
• Reference material which peaks at one of the instrument's wavelengths.
• Precise 1:2 serial dilution of the reference material.
1. Ensure that the Flowcell is active.
2. Place a 12 mm borosilicate test tube filled with 1 ml of water into the read
well.
3. Select TOOLS, Diagnostics, FILTER VOLTAGES. Confirm that all of the filter
voltages are between 2.00 and 10.00 volts. Refer to Section 4.2 Photometer
Problems if voltages are not within this range.
Never adjust trim pots on the Main PCB to achieve the proper voltages.

4. Determine the absorbance of both the 1:1 reference material and the 1:2
dilution on a reference spectrophotometer. The 1:2 dilution should be in the
range of .8 to 1.2 absorbance. The 1:1 material should be in the range of 1.6
to 2.4 absorbance.
5. Remove the tube and install the Flowcell in the instrument. Press Program to
create an ABS test. Select the required wavelengths. Blank on the same
diluent material used to prepare the concentrations.
6. Read the 1:2 dilution. Divide the absorbance as read on the reference
spectrophotometer by the reported absorbance value.
7. Exit the absorbance mode by pressing QUIT to return to Main Menu. Press
TOOLS, DIAGNOSTICS, Absorbance Calibration. At the prompt
“Absorbance factor= x.xxx?”, enter the ratio calculated. When this factor is
entered the instrument will display a net factor (the new calibration data).
8. Press QUIT and restart the absorbance mode. Blank as before on the
diluent. The 1:2 dilution absorbance should now agree with the reference to
within +/-.005 absorbance.
9. Read the 1:1 concentration. It should agree within +/-2 % of the reference. If
necessary, carefully adjust trimpot TP3 to achieve this agreement. Re-blank
and reconfirm the values.
10. Select test #213 to print the new calibration data. Enter these values on the
calibration data label on the bottom of the instrument.
5.5 Restore Filter Labels

Like the calibration data, the wavelengths for the two optional filters are stored in
nonvolatile memory. In the event this data is lost or corrupted, the following message will
be displayed and printed.
Filter Labels 7&8 Clrd!
Filter labels need to be re-entered for two of the filters. Open the instrument and locate
the filter label on the side of the photometer cover.
Key 7 is xxx
Key 8 is xxx
Where “xxx” is a three-digit wavelength value. If there are no 7th or 8th filters, they will be
listed as BLOCKED. Press RUN TEST (F1), type in 248, and press ENTER. The display
prompts:
Key 7 = ??? nm
Type in the wavelength for Key 7 that is printed on the label and press ENTER. Repeat
for Key 8. use “000” for unused filter positions. Press QUIT to return to the main prompt.
Note that, if values for Key 7 and Key 8 are entered when there are no filters present, the
filters will be flagged as “low” when Self Check is run.

6. CONTACT INFORMATION
If you continue to have problems after consulting your dealer, contact the factory.
Phone: USA 772-283-6540
Fax: USA 772-283-8020
E-mail: support@awaretech.com
Mailing Address: Awareness Technology, Inc.
P.O. Drawer 1679
Palm City, FL. 34991
USA
When contacting us, please have serial number of the Stat Fax® 3300 in
question. Have a description of the problem with as much detail as possible.
Save any relevant jobs or logs to disk and send or e-mail us the information.

APPENDIX 1 – SCHEMATICS

Layout

Layout (Continued)

Layout (Continued)

Notes:

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©Copyright 2004 Awareness Technology Inc. 01/2008 Doc. 3300 Rev. G

1.1.2 Summary of the Instrument

Awareness Technology Inc. Stat Fax® 3300 Service Manual Rev G 1

2 Stat Fax® 3300 Service Manual Rev G Awareness Technology Inc.

Awareness Technology Inc. Stat Fax® 3300 Service Manual Rev G 3

Design and Instrument specifications are subject to change without notice.

4 Stat Fax® 3300 Service Manual Rev G Awareness Technology Inc.

WARNING Protective Ground CAUTION BIOHAZARD

1.3.2 Safety Terms

DANGER indicates an injury immediately accessible as you read the

WARNING indicates an injury hazard not immediately accessible as you read

CAUTION indicates a hazard to property including the product.

Terms which may appear in this manual:

WARNING: Warning statements identify conditions or practices that could

CAUTION: Caution statements identify conditions or practices that could

BIOHAZARD: Biohazards are biological agents that can cause disease in

1.4 Safety Precautions

Personal Protective Equipment

Awareness Technology Inc. Stat Fax® 3300 Service Manual Rev G 5

Use Proper Power Cord

Ground the Product

Observe All Terminal Ratings

Provide Proper Ventilation

Do Not Operate Without Covers

Avoid Exposed Circuitry

Avoid Excessive Dust

Do Not Operate With Suspected Failures

Do Not Operate in Wet/Damp Conditions.

Do Not Operate In An Explosive Atmosphere.

6 Stat Fax® 3300 Service Manual Rev G Awareness Technology Inc.

WARNING: If any materials are overturned during operation, immediately

Awareness Technology Inc. Stat Fax® 3300 Service Manual Rev G 7

2.1 Power Requirements

Voltage source: .........................................90 – 264

8 Stat Fax® 3300 Service Manual Rev G Awareness Technology Inc.

Figure 2.2-1 Overall view

Figure 2.2-2 Rear view

Awareness Technology Inc. Stat Fax® 3300 Service Manual Rev G 9

Figure 2.2-3 Waste Bottle Assembly

Reference Figures 2.2-1, 2.2-2, and 2.2-3:

A. Flowcell M. Sensor Jack

10 Stat Fax® 3300 Service Manual Rev G Awareness Technology Inc.

Cover. The cover is a pressure formed fire-retardant ABS enclosure meeting UL

2.3.2 Front Panel Electronics

The keypad: The keypad consists of a 5 X 4 membrane keypad. Ports PB0

Awareness Technology Inc. Stat Fax® 3300 Service Manual Rev G 11

Figure 2.3.3.1 – Surface Mount Main PCB Configuration (Rev. L artwork)

Diode Label Function

12 Stat Fax® 3300 Service Manual Rev G Awareness Technology Inc.

Figure 2.3.3-2 - Functions indicated by 7-segment LED

The 7-segment LED on the Main PCB displays functions as shown in

Pump Indicates a signal to turn on the pump.

Sample Sensor switch Indicates when the sample sensor

Cell heat The heating elements in the

Tube detect A test tube or Flowcell is inserted into

Power +15V available on Main PCB.

Valve action A signal to open the pinch valve is

Awareness Technology Inc. Stat Fax® 3300 Service Manual Rev G 13

The photometer is operated under microprocessor control. The filter wheel is

The photometer mechanism consists of four elements:

• Filter wheel position detection system: Optical gates (beam interrupt

• Optical detection system: This system consists of a photometer PCB

Numeric Key 1 Read Water Reference:

Numeric Key 2 Flowcell Alignment:

Numeric Key 2 Flowcell Alignment: (Continued)