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MCQ FOR GENE TRANSFER

1. Ti plasmid are used to create transgenic ______.

A Plants

B Animals

C Fungi

D All of the above

2. The Ti plasmid is obtained from which species?

A Escherichia coli

B Agrobacterium tumifaciens

C Thermus aquaticus

D Bacillus thuriengiensis

3. Which vector would you chose to express a foreign gene in a plant?

A Baculovirus vector.

B Retroviral vector.

C Yep vector.

D Ti vector having T-DNA

4. pBIN 19 binary vector consist of

A lac z' gene

B Kanamycin resistance gene
C Cloning sites
D All of above
5. Which one is not used in making genetically modified crops ?
A Vector based on plant Virus
B Various plasmid DNA
C Artificial plasmid of animals
D Natural plasmid of Agrobacterium
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6. Which is not a method of DNA insertion

A Transformation
B Transfection
C Microinjection
D None
7. Agrobacterium tumifaciens causes
A Rust
B Mildew
C Crown Gall disease
D Hairy root disease
8. Plant cells can easily regenerate into entire plant because
A These are totipotent
B Embryo is totipotent
C Stem cells are pluripotent
D They divide actively
9. Which of the following microorganisms has been widely used as a vehicle for foreign gene
transfer in plants ?
A Agrobacterium
B Escherichia coli
C Bacillus anthracis
D TMV
10. Ti plasmid is present in
A Agrobacterium
B Cymbidium
C Dendrobium
D Syzigium
11. The first transgenic crop produced was
A Pea
B Flax
C Tobacco
D Cotton
12. Insect tolerant gene from Bacillus thurngiensis is introduced into plants using plasmid of
A Escherichia coli
B Agrobacterium rhizogenes
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C Haemophilus influenza
D Arabidiopsis thaliana
13.Agrobacterium tumifaciens is an effective vector for use with
A Corn
B Rice
C Wheat
D Soyabean
14. What roles do opines play in crown gall diseases ?

A Source of Carbon, Nitrogen and energy for the Agrobacterium

B Transfer of T-DNA to plant cells

C Attachment of Agrobacterium to the plants

D Induction of expression of Vir genes

15. A plant Genetic Engineer wishes to transfer and express a gene from Sunflower into
beans,which of the following would be vector of choice ?

A lambda phage

B pBR322

C Ti plasmid

D Maize streak virus

16. Golden Rice is a transgenic crop with a trait for

A Pest Resistance

B High vitamin A content

C Drought Resistance

D High protein content

17. Transfer of T-DNA from Ti plasmid into plant cell is mediated by

A mob genes

B vir genes

C nif genes

D octapine genes