• EVALUATION OF IRON STATUS IN WELCOME

PREGNANT WOMEN OF
NORTHERN KERALA
 AIM AND OBJECTIVES
PRIMARY OBJECTIVE
TO EVALUATE THE IRON STATUS OF PREGNANT WOMEN OF NORTHERN KERALA

SECONDARY OBJECTIVES
1. TO COMPARE IRON LEVEL WITH HEMOGLOBIN
2. TO FIND OUT THE CORRELATION OF IRON LEVELS WITH SERUM CALCIUM AND
PHOSPHORUS LEVELS
3. TO FIND OUT THE VARIATIONS IN HEMATOLOGICALPARAMETERS IN PREGNANT
WOMEN FROM NON PREGNANT WOMEN
4. TO ASSESS THE USE OF IRON STATUS FOR ESTABLISHING IRON DEFICIENCY
ANEMIA IN PREGNANT WOMEN
5. TO ESTABLISH IRON STATUS AS A ROUTINE INVEATIGATION IN PREGNANT
WOMEN WITH IRON DEFICIENCY ANEMIA
 MATERIALS AND METHODS
THE PATIENTS CHOSEN FOR THE STUDY WERE PREGNANT (TEST) AND NON
PREGNANT (CONTROL) ATTENDING THE GYNECOLOGY OP AND MEDICINE OP
OF ACADEMY OF MEDICAL SCIENCES, PARIYARAM. IN THIS STUDY 100
PATIENTS WHO WERE PREGNANT AND 35 NORMAL HEALTHY WOMEN OF
REPRODUCTIVE AGE WERE TAKEN AS CONTROLS.

INTRUMENTS USED
1. SYSTRONICS UV VISIBLE SPECTROPHOTOMETER
2. CELL-DYN 32OO FULLY AUTOMATED ANALYSER
3. ROTEK LABORATORY CENTRIFUGE M8E
4. TABLE TOP CENTRIFUGE (REMI)
5. INCUBATOR
 ESTIMATION OF SERUM IRON
 FERROZINE METHOD

ASSAY PARAMETERS
 ESTIMATION OF SERUM TOTAL IRON BINDING
CAPACITY (TIBC)
FERROZINE METHOD
THE SERUM IS TREATED WITH EXCESS OF FE+2 TO SATURATE THE IRON BINDING
SITES ON TRANSFERRIN. THE EXCESS OF FE+2 IS ABSORBED AND PRECIPITATED
AND THE IRON CONTENT IN THE SUPERNATANT IS MEASURED TO GIVE TIBC.
ASSAY PARAMETERS
CALCULATION OF TRANSFERRIN AND
TRANSFERIN SATURATION
 ESTIMATION OF SERUM C ALCIUM
0- CRESOLPHTHALEIN COMPLEXONE, END POINT ASSAY

ASSAY PARAMETERS
 ESTIMATION OF SERUM PHOSPHORUS
UV MOLYBDATE, END POINT ASSAY

ASSAY PARAMETERS
ESTIMATION OF HEMATOLOGICAL PARAMETERS
HEMATOLOGICAL PARAMETERS SUCH AS RBC COUNT, MCV, HCT, MCH,
MCHC WERE ESTIMATED USING ABBOTT-CELL-DYN 3200 FULLY AUTOMATED
ANALYSER.
 RESULTS
 IRON LEVELS IN TEST AND CONTROL
140

120

100

values
80

60

40

20

0
control stage1 stage2 stage3
TIBC LEVELS IN TEST AND CONTROL

500

450

400

values
350

300

250

200

150

100

50

0
control stage1 stage2 stage3
TRANSFERRIN LEVELS IN TEST AND CONTROL

3.5

2.5

values
2

1.5

0.5

0
control stage1 stage2 stage3
TRANSFERRIN SATURATION LEVELS IN TEST AND CONTROL

35

30

25

20

values
15

10

0
control stage1 stage2 stage3
CALCIUM LEVELS IN TEST AND CONTROL
9.7

9.6

9.5

values
9.4

9.3

9.2

9.1

8.9
control stage1 stage2 stage3
PHOSPHORUS LEVELS IN TEST AND CONTROL
4

3.9

3.8

values
3.7

3.6

3.5

3.4
control stage1 stage2 stage3
HEMOGLOBIN LEVELS IN TEST AND CONTROL
12.4

12.2

12

11.8

values
11.6

11.4

11.2

11

10.8
control stage1 stage2 stage3
RBC COUNTS IN TEST AND CONTROL
4.5

4.4

4.3

4.2

4.1

values
4

3.9

3.8

3.7

3.6

3.5
control stage1 stage2 stage3
PCV LEVELS IN TEST AND CONTROL
45

40

35

30

25

values
20

15

10

0
control stage1 stage2 stage3
MCHC LEVELS IN TEST AND CONTROL
38

37

36

35

34

values
33

32

31

30

29

28
control stage1 stage2 stage3
MCH LEVELS IN TEST AND CONTROL
29

28.5

28

values
27.5

27

26.5
control stage1 stage2 stage3