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BIO - Ummehani.m.

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Title:  The effects of temperature on the rate of


reaction (amount of foam produced during the
reaction of hydrogen peroxide with yeast solution) 
 
Variables: 
 Independent Variable: temperature
 
Dependent Variable: the amount of foam produced (rate of reaction) 

Control Variables Table: table 1

Control Units Why is it How should it Has it been


variable controlled? be controlled? included in the
method
pH - Change in the The amount of Yes
pH will affect pH can
the rate of controlled by
reaction using the buffer
solution and
measured using
pH paper
BUFFEER 20ml Used to maintain The amount of Yes
solution 7 the pH 7 can controlled by
measuring using
the cylinder or
pipet
hydrogen 20ml High The amount of Yes
peroxide concentration of pH can
hydrogen controlled by
peroxide can measuring using
reduce the the cylinder or
catalytic reaction pipet accurately
yeast solution 5g in 10ml of Yeast solution The water and Yes
water will work as a yeast solution
catalyst so the will be kept in a
decrease in smaller test tube
concentration the amount is at
will decrease the 10ml of the
rate of reaction Measure the
amount of yeast
using an
electronic
balance
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Research Question:
How does the change in temperature affect the rate of catalytic
reaction?

Background Information:

A catalyst is an agent or a compound that speeds up an experiment .A


catalyst can increase the rate of the reaction and can participate in
secondary reactions without suffering itself for any permanent chemical
change.

The effect of temperature plays a high role with a catalase reaction with
an increase in temperature this gives us a guarantee that the reaction
will a successful reaction.This will result in more particles with higher
energy. This means the higher the temperature the higher the rate of
reaction. However having extreme high temperatures the rate could
decrease due to having been degraded by the high temperature.

In this investigation we will see the how the catalyst reacts to different
temperatures this reaction will be seen in a physical change .To
measure the rate we will measure the volume of how much foam it
produced.

In this experiment we will see how using yeast as a catalyst react to


different temperatures. This reaction will be seen in a physical change
the way we can see is through the reaction between yeast solution and
a hydrogen peroxide plus pH buffer solution the reaction will cause a
foam like substance to be produced by measuring this substance we
can tell that the rate is fast or slow.

For a reaction to occur between particles they must overcome a barrier


this is called the activation energy. When there are particles with higher
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energy then the activation energy there is a higher chance of having a


successful reaction

Hypothesis:
If the increase in temperature will increase the reaction at a certain
limit, above that the rate of reaction will start decreasing due to
degradation of catalyst.

Materials Table: table 2


Material Units Quantity Uncertainty
Yeast 5 grams 5 grams ±0.5
pH 20 milliters 20 milliters ±0.5
Buffer solution 7 20 milliters 20 milliters ±0.5
Hydrogen peroxide 20 milliters 20 milliters ±0.5
Water 20 milliters 20 milliters ±0.5
Graduated cylinder - 5 -
Test tubes - 5 -
Water bath - 5 -
pH paper - 5 -
Electronic balance - 1 -
Test tube holder - 1 -
Thermometer - 5 -

Gloves - 2 pairs -
Lab coat - 2 -
Goggles - 2 -

Safety, ethical and environmental concerns:


Risk posed Precaution taken
Safety The chemicals could fall on wear lab coats, gloves and
to the skin or on someone’s goggles and do not use
clothes or may even get into corrosive or extremely
someone’s eyes harmful chemicals
Ethical - There are no animals taken
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Risk posed Precaution taken


into use of this experiment
Environmental Harmful gases could be This experiment will be
created that may pollute the indoor so if a harmful
air substance is created there will
be a way to contain that.

Method:
Wear the lab coat, goggles and gloves to protect the skin from harmful
chemicals

By using the electronic balance measure 5g of yeast and measure


10ml of water using the cylinder add both in a beaker stir it well to
create a homogenous solution.

Take the 20 ml of buffer solution of pH 7 using the pipet and put it in a


test tube.

Using the pipet again take 20ml of hydrogen peroxide solution and add
it into the test-tube containing buffer solution
Place the graduated cylinder and test-tube in 5 different water bath all
maintaining different temperatures such as: 20°c, 25°c, 30°c, 35°c, 40°c,

Keep checking the temperature of the water baths with a thermometer


Leave the test-tubes and cylinder for 10min in the water bath for
acclimatization of solutions.

Use the test tube holder to take them out .For each cylinder add 10ml
of yeast solution all at once and wait for 20 seconds to produce
maximum foam in each cylinder.
Using the scale notice the amount of foam produced in each cylinder
and notes it down in table.

Repeat the experiment unto 5 trials to make the result more reliable.

Bibliography;
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https://www.mytutor.co.uk/answers/13239/A-Level/Chemistry/How-
does-a-catalyst-and-increasing-temperature-affect-the-rate-of-reaction/

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