Plate ID:

Step 1: fill-in Plate ID (no spaces) Sample ID Format OK? OK

Step 2: fill-in Sample ID (no spaces or

symbols) 2 Wells Labeled: PGEM PGEM WELLS NOT OK

Well Sample ID:

A01 0
B01 0
C01 0 PLEASE READ AND FOLLOW THESE INSTRUCTI
D01 0
E01 0 Note: Input information into light yellow boxes only.
F01 0
G01 0
H01 0 PLATE ID
A02 0
B02 0 Step 1) In the "Plate ID" box, write Researcher's 3 init
and starting plate number. (e.g. ABC##). Spaces are n
C02 0
allowed in the Plate ID, due to restrictions of the ABI so
D02 0
Also write this plate ID on the side of the plate itself.
E02 0
F02 0 If you are submitting multiple plates, complete this enti
G02 0 workbook for each plate, and submit a separate online
H02 0 form for each plate submitted.
A03 0
B03 0
C03 0
D03 0 SAMPLE ID
E03 0
F03 0 Step 2) In the 96 cells corresponding to the wells, inpu
sample IDs for the 96 wells of the plate. This can be ea
G03 0
accomplished by pasting a column of information from a
H03 0
existing spreadsheet. Please paste values only (not form
A04 0 using the PASTE SPECIAL function in Excel (RIGHT CL
B04 0 select PASTE SPECIAL, select values, select OK). Leav
C04 0 empty if well is unused and clean. Periods or other
D04 0 characters are not allowed except underscores.
E04 0
Partial plates should be filled from A1-H1, A2-H2, etc. le
F04 0
Blanks at the end of the plate.
G04 0
H04 0
A05 0
B05 0
C05 0 BLANKS (PGEM)
D05 0
E05 0 Step 3) 2 Blank wells are needed for PGEM (contr
Indicate 2 blank wells by writing PGEM exactly as writte
F05 0
for 2 controls that are required for all plates. The indica
G05 0
will read "OK" if you have entered two empty PGEM wel
H05 0
A06 0 NOTE: THIS IS VERY IMPORTANT! Every plate must hav
B06 0 uniquely located empty wells (no water, no buffer -- no
C06 0 other words, these two wells must not be the same on
D06 0 plates. Ideally, they should also not be symmetrical abo
diagonal (i.e., don't use A01 and H12, as if a plate is in
E06 0
these blanks do not diagnose such an error). These blan
F06 0 locations will be used for positive control template (PGE
G06 0 plasmid), and their orientation will therefore serve as a
H06 0 guarantee of sample plate ID throughout the entire pro
A07 0
B07 0 Blank wells are to be left empty, without water or buff
C07 0 the Sample ID cell blank on the submission form and do
write anything in the cells that do not contain sample.
D07 0
when a blank well appears within other samples and is
E07 0 PGEM, you will need to label that well/cell (ex: BLANK,
F07 0 Nothing).
G07 0
H07 0
A08 0
B08 0
C08 0
D08 0
E08 0
F08 0
G08 0
H08 0
FAILURE TO FOLLOW THESE INSTRUCTIONS
A09 0
RESULT IN A DELAY OF YOUR PROJECT
B09 0
C09 0
D09 0
E09 0
F09 0
G09 0
H09 0
A10 0
B10 0
C10 0
D10 0
E10 0
F10 0
G10 0
H10 0
A11 0
B11 0
C11 0
D11 0
E11 0
F11 0
G11 0
H11 0
A12 0
B12 0
C12 0
D12 0
E12 0
F12 0
G12 0
H12 0
 OK

PGEM WELLS NOT OK

OW THESE INSTRUCTIONS

ght yellow boxes only.

Row-
ordered
Column-
(A01, A02,
ordered
3
A03
(A01,…)
B01,
write Researcher's 3 initials and
g. ABC##). Spaces are not C01 …)
restrictions of the ABI software.
side of the plate itself.

plates, complete this entire Excel

ubmit a separate online order

ponding to the wells, input 96

the plate. This can be easily
mn of information from an
aste values only (not formatting)
nction in Excel (RIGHT CLICK
alues, select OK). Leave cell
clean. Periods or other special
ept underscores.

om A1-H1, A2-H2, etc. leaving

eeded for PGEM (controls).
g PGEM exactly as written here
for all plates. The indicator above
red two empty PGEM wells.

NT! Every plate must have two

no water, no buffer -- nothing). In
must not be the same on any two
o not be symmetrical about the
nd H12, as if a plate is inverted,
uch an error). These blank wells'
ve control template (PGEM
will therefore serve as a
hroughout the entire process.

pty, without water or buffer. Leave

e submission form and do not
do not contain sample. ONLY
hin other samples and is not a
at well/cell (ex: BLANK, EMPTY,

HESE INSTRUCTIONS WILL

Y OF YOUR PROJECT.
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
1 2 3
2 A01 A01
3 A02 B01
4 A03 C01
5 A04 D01
6 A05 E01
7 A06 F01
8 A07 G01
9 A08 H01
10 A09 A02
11 A10 B02
12 A11 C02
13 A12 D02
14 B01 E02
15 B02 F02
16 B03 G02
17 B04 H02
18 B05 A03
19 B06 B03
20 B07 C03
21 B08 D03
22 B09 E03
23 B10 F03
24 B11 G03
25 B12 H03
26 C01 A04
27 C02 B04
28 C03 C04
29 C04 D04
30 C05 E04
31 C06 F04
32 C07 G04
C08 H04
C09 A05
C10 B05
C11 C05
C12 D05
D01 E05
D02 F05
D03 G05
D04 H05
D05 A06
D06 B06
D07 C06
D08 D06
D09 E06
D10 F06
D11 G06
D12 H06
E01 A07
E02 B07
E03 C07
E04 D07
E05 E07
E06 F07
E07 G07
E08 H07
E09 A08
E10 B08
E11 C08
E12 D08
F01 E08
F02 F08
F03 G08
F04 H08
F05 A09
F06 B09
F07 C09
F08 D09
F09 E09
F10 F09
F11 G09
F12 H09
G01 A10
G02 B10
G03 C10
G04 D10
G05 E10
G06 F10
G07 G10
G08 H10
G09 A11
G10 B11
G11 C11
G12 D11
H01 E11
H02 F11
H03 G11
H04 H11
H05 A12
H06 B12
H07 C12
H08 D12
H09 E12
H10 F12
H11 G12
H12 H12