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    This document is an SDS-PAGE gel analysis of purified wild type and mutant proteins. It shows the molecular weights of the wild type protein and two mutant variants at approximately 45 kDa. A third mutant variant is also present at the same molecular weight. The gel demonstrates that the proteins were successfully purified for further analysis.

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    electrophorese

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    pone.0147301.s001.pdf

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    This document is an SDS-PAGE gel analysis of purified wild type and mutant proteins. It shows the molecular weights of the wild type protein and two mutant variants at approximately 45 kDa. A third mutant variant is also present at the same molecular weight. The gel demonstrates that the proteins were successfully purified for further analysis.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    21 views1 page

    Pone 0147301 s001 PDF

    Uploaded by

    lop
    This document is an SDS-PAGE gel analysis of purified wild type and mutant proteins. It shows the molecular weights of the wild type protein and two mutant variants at approximately 45 kDa. A third mutant variant is also present at the same molecular weight. The gel demonstrates that the proteins were successfully purified for further analysis.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 1

    t

    an
    ut
    2/ nt
    m
    17 ut a

    8
    33
    16 2 m
    17
    7/
    7/
    M

    T
    M

    W
    16
    (kDa)

    200
    116
    97

    66

    45

    31

    S1 Fig. SDS-PAGE analysis of the purified WT and the 167/172 and

    167/172/338 mutants. The recombinant enzymes (5 µg each) were subjected

    to electrophoresis. M, molecular mass markers.

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