194 200 24251 Journal of Hospital Infection Review Persistence of coronaviruses on inanimate surfaces and their inactivation with biocidal agents G. Kampf", D. Todt, S, Pfaender”, E. Steinmann? * uivesey Medicine Geena, Intute for Hygiene ond Environmental Medicine, Ferdinand Saveronuh-Sroe, 17475 Grefoate, Germany Department of Molecular and Media! Vly, Ruby Unveity Bochum, Universitatea So, 4901 Bochum, Gemany Bedeniage Garey Se merges avo has coarviy RACO, Tones gal Decepted 31 Janaty 2020 Usamistor have en casein ao fatre a0 lt avlaste formation about We pesstence of hunen and weternary er on marimate turner at wall ar craton aeons get Remora ‘es. nesthcareTacives. Thana of 72 set Corman feveale shat nan cororavses ach Severe Acts Resortary Spon aR Perstence avin, ede ask Rexpratry Syndrome (HRS) crerai or sneer Manan ‘Chemical inactivation ‘but can be eficlenty activated by surface disinfection procedures with 62—71% Bia ages fal 3.8 nyone eroaeo sadm yaar hin | Pe: Introduction spread in the public and healthcare stings. Fransmsson of ‘A novel coronavius SARS-COV.) has recently emerged ‘rom China wth 9 ttal of 4517 confirmed exter of pro trons (ar of February 12,2029) (1 Together wit Seve este Respiratory Synarome (S435) coronavrur and Milo East Respiratory Syncomme ERS) coronvins [2], OSs the third highly pathoterc Nanan coronevi that hos neta Inthe tas two cecadee Prson-to-peson EAaSMsion Mas Seen described both ir hospi! and amily Settings [is Ahrefore of umost Importance to prevent any fer romper 6 Kamp eis aan 01.2 Dortuated nein stnaclaion of mucour membranes iF the note eyes mouth [és], emphasing the impo {ince ofa cea uncertandng of corenavi perstence cents surtces [ vars pe of Noa aes Berzalkonun chlonde are wsee wortwae for Sahecton, ry nena tg Tn ti of he even oF al cornaruss nly eneing SARS-CoV a HERS CoV as wll ar veterinary coronaire sich a anenierble Ssvoenterts veut {TEE}, mouse hepetits vrs HY) Sha canine coonevror (2A). an erent per oF eller fctin Sct, Pale by Eerie sed6. Map e al Jourel of Hosa Infection 106 (2020) 246-251 Blocial agents tsee sm surface esnectans against Method folowing seert were ured, aye fe combination with "aronaas", "GEV", "Mor "CCV" sual sua (68 pesstencenandi8/0/3/ his, survvathand 22/0731 ta surraakin(8/0/0/ 1M} ersterceshn (0/0. Th) vrocda 233/37 nes: remielractratn 23 7 07 6/1) suaperson test (180707 ON ander a (17 141 01 Oe. Pobleatant wore icles td reste wore “enuactd gen they peovoed egal deta on caveats ob parton faces, materia) td raciation by boat [gens wed for dsinvcton (uspension tests, camer at, finan sts). ata with eommertl products sre of ‘nor serene typer of lecda agent were excldes within tn spe ofthis revi. THpeot aria Tne a Fae amie Rey Ste2¥¢ argc oe saas.coy sean Key Sea 29 Surge aave (ates) HESY Sans 229 and 0C43 connie Fev stan me Results Persistence of coronavirus on inanimate surfaces est data were decree with the endemic hunan core: sais eas HC] 229 On dere pe of atl e an remain irfectus for fom 2 hous up f09 days A Maer persistence of Why pathogenic MERS-CoV, TGEV and MAY. Honea acpesence G8 nd WV ce ese Inet at prtence was oper wh ahr ecla {Tate lh tddon was shown a oun temperate ts Scav.ae peste betr at S08 compared vo 30 rete mara Inactivation of corenaviruses by biocidal agents in suspension tests ane (74-95%), 2-propano 70-1008), the combination of 45H Dprapanal ite JO” ‘repel, sluardaldenyee TOsc2sk), formaldehyde (O7A18) are poveone taahne 1° We Tr ve 0 ey we ry Seo ba ie ts ° bs wo ts fa ba e i ia ba ie Gy i ba wo ta fa bo e Pa o ba wo ty fy by Se ta e ba e i e ae we @ re (3 Tens = mee Ei Repro Spine, Heo = punan cwerean TOV Stas Severe ass Reprnny sya! AT ~ ann angesoa 6, Kamp el arnt of Hostal Infection 1042020) 246-251 tae me vas Suen iste Bgaiwetine—_teaicioneT oes mx coy Sant tom 333 bo 2reparal fox Eatecoy sate et a! 533 by me Saco hottest wos 54 ba ES NeKScov Stan ae 2 ao ba, hos Sanecev te Pt ee 33 ba. om av Stara 2 fomn 537 bay sox coy Sant tom 337 ba. 2erparal and UShane HX SARS-COV late mt a 2a bo propa! Saco hatte Pact wee 228 rat enalonin corde 0.28 Ney aTceRIS9 omin oo ba 58 wav Sta 2 toms > 37 bo. ons coy Sant tom a7 bal Sanne Gey Sansire uM ao by Daccyemotns. Sams Ey Stan PE a4 24a ba chanendine oom mw sansa tom bal om co fino tome pol Seatum hpacionte 0218 iy Stam te wes ba oa ft Semana tome ba, Hsosen pede 0.58 Hey stan 2 tine oe 025% SARS-COV tn 40 [aS sr tae Rao aOR MENS = leae a sa aaat 7 [San SScL EE HE cs 7 a ‘kon u/minaract ean da ba 5 2 er im = is # th = 2 ec oe i fe He = toe Barmera erm a vewtonncinnie Soa me Sem, Seem ie ‘a compa O88 REE BAVeeoes ess im S38 i othe ‘Mey Baruyr eae i om Stee see me imo a cwensinee fo AE Bavaees Gemini ii 8 Sree Fase Sa ecm se is ce mS on Mw oom Styl /anmesstect Nene se 0 by & a SS TS '250 6. Mampy et Journal of Hostal Infection 1042020) 246-251 (022-754) readty facthated coroavis infectivity by approximately $topic or more. (a=) Socian hypecnore ‘eguved @ minal concetaion of at leat OFT to be fective. Hyrogen peronde was effecive with 2 concen tat of 3% ant cibation reo Yin Da obtalned ‘wn bonetenkum elage at restorableconact tires wore ating With 10 min a ceentabon of 0.2% reveated no ‘tfeny agar carnavins whereas concentration af 0.05% vine ste effete, 0.02% chomhenidne.dpuconte wae Basel tnttective Tab). Inactivation of coronaviruses by biocdal agents in carrier tests Ethanol at concentrations betwesn 628 and 71X reduced corona tethy win tin expasre ine by 20-40 logy. concertation of, -0.58sacum hypochiate and 28 sdaidalaehyce were ao que eectve wi 3.0 ee Feuer sn vial tre. Ir contas, 004% Berzaeonm horde, 000% sedium hypecNorte and 05% orth. prtalaldehyde were tea tect (72 lh. Discussion Human cernavres can remaia fective 07 inanimate safes arom temperature or up 109 day. At 8 tee ‘tire of °C er mare te durations perestence store ‘Veterinary coronas nave beer shown to pest eves longer fo" 28 Contamination of frequent tosh maces 9 heatrcare setngs are erefore a ater Source ef ir. UWarsmasion Daan the rani crores rom “ontamatee surfaces fo hance were nt found. Howeve’, i Ianster 3.68 of the wa oat the hance]. Te tranaer iflene was ower (158) with peaauenea vs Sanda 53 “antaet between the rfce and he hands], han bee ‘ational stay twas sero Bat student ou he ace ‘on oe own tars ah average 73 tes per hy with cotact ‘ost fo tho skin (43), flowed by mouth (64), rose OTS) Sheejes 3%)" Although the val ed of arenas on ‘tanimate earacere ot known dorng an outbrearstntion sce plas to recuce the vial lad on suraces by di Infectan, especialy of fequenty toached surfaces in the Inmeclte sent suroucing where he highestvra aden bbe expected. The WHO rearmands "to emu tat enor imental clear and. denfecten procedwrer re followed anatenty and correctly. Tarogty cleans envionment Surfaces wh water ang cetergent ng appinng commeniy {sed nepal evel cfectars such as soctum pect) Be effective ana sufitont procedures." [12] The tpl we ‘ot beach fat 3 tion of 1:10 of 5k soclun hypechrte ‘eauling nara concentration of 0.058. Cursumnar zed ‘ata wth crcraveuss sages tata cncentration of 155 tlc isis). TRak why escems appropriate {errcomanc alin 150 of standard leach the cos ‘aves Sling. Far te dsecton of sal surfaces ethane (Ga=7ix; caror toms) reveseg a sma effeny agsinet ‘mended by the WHO for einfocting sal suraces No dita were found to describe the frequency of hands ‘econ contaminated with erona, or he va ado hands either, after patient contactor after touring ca {aminated surface, Tne WHO reconenendr fo preferably apy ‘Scthactased hand rubs fr the decantarnation of hand, Sig after romowng gover, Two WHO recommended fo. ‘ruaton (aceon 6 ethanol” 7K Z-propara) have been ‘raat ln sispnsion tests apt SARS-COV ar MENS, Se both ware ezerbed tobe very etfectve (1) No no date were found on the eesey of hd wating apt “ornavr contamination on hansen Tata, Powerer, ras decribed tha taling hand wash stain the emer {oncy department wnt the ony infection control measure Vehen was sigfeany assocted Wty the potecton Tamn Fealtheare workers fram aeqitg the SARSCOY, naeatng {hat hand hygiene can havea pretectvee'fect [5] Corr Dilance it hand hygiene can be sgieaty ges fn an ‘Sutbeak stain buts rel to remain an bstcl expetaly Song prin [e~2] Taramison in heats et gt ‘ane Focersdly preverte when appreaate mesures are ‘sitenty performed [9,2 Conclusions Human corrarses can rein infective on ranenate sree or upto days. Surface dafcton with. sodium Iypechionte er 62-71X etanal gran reduces corona ‘ie lfectvty on surfaces tnt min exposure te. Me fexseet sa effect agains he SARS-COV Conic of interest statement None eecared Funding Sources References 1m ne, coma Bese 209 fom WHO 228 Stn Ret Day Set an Oaremaien Warr 8 rer VSS nd Se eet nee rewrite 1] En 5 Th Yt a aa inca perro Caran a yf fy Sener anet Ie. hep ee oto ea 1m ter Si, Socey , Yea, Ontbwake , Goldener 5, 1B] Domes sr, Sinmerman JM, Cra 00, Wu, chaorevanich A Ia sa se seen yoo coe (0 ter arava RE. ean cee: he 1 Farstcanept stewart Bocce etree 2 eal fnpcane chan Speer neta snes 08 1 Set StS rg Convio wes ha wi She BSB Pee A, Cavin6, Kamp eal ural of Hospital Infection 104 (2070) 246-251 ast 10 oat Sk, Se Ns Sa A ae aba 1 Roa Ys to ea Fae tung a agar ae {hot ha imtaten ar hard yen rn es Cote fitter pevertin a coal ic heat cre when tet Cori cin ope. Wi, 09 Wr, Won an eect sed ap ene. Se pin te cn cr prec pere (a Bw ne vt tana Fed on ncn ene [16] Ashammani te, Reynolds KA, Vorhougstracte M, O'Rourke Mt Sees eer ren caee ee eee in es etre i htt eg eee 1 Se annie re doer ms meaeeae ocean tones eas ee eee [20] 0, Han 5K, Son 46, Park SO Rk of wana va medial Poeneiipeiera ae omcaa hacocrarn ner [BM] van Berean H, BushraberT, Master V3, Sabity of mie amie haere LS Seeetama reer oa inns etack nna See res eeataeeenre e sorta vas a BERET are ee (Bay Sian 3, Yu mw, Tbr ea se ang an acon spss (tn ge cen Eee ahaa Wace at pas Satta dacs tes on S's haan ea aw he a or WSs ane acai of SH coo hed saben a Gy Sea Der tency of vis BO) Sok el cnet to engenaeane Set Caan deat eeeaiiee |B] Desianno C, Vous @, Boesenborg B. The antiviral action of com- soanhoeaiety mule acts (24 omni star sad nei messi conic saa ows ae pata enie oen S rections. 3 Hoy wee ‘eeaisl isc Smczaana) Re RN (BM Lggen Ekman, Zor J, Rapid ae ec Va ‘lyst Porconane Prost An Me a Sep {str Sarone Caen NERS Co reefs Voce Via ar pi ase De her 235 -494-S (Bm ges he fabeertsen Eckman Zor Vise ‘esr ard Vr tfcacy of Povaonene Cage enn ast penta OT abe Ht (Bm ther Ry Csarora UM, Rtn WA, Weber Sey HO. Inetan st surapts cevan sno curtsr sy eh 1) pra arn Lae, onc ‘peed rian ene ter epee (48 Ga ‘Mayorconn peracid apr Maple