CERTIFICATE OF ANALYSIS

Product Name: Xylose Lysine Deoxycholate Agar Plate (Harmonized)

Lot No.:P14-2002
P14-2005 Catalogue No.: 205240080020/205240080100

Mfg.: 10-02-2020
02-06-2020 10-02-2020 Exp.: 02-09-2020
05-06-2020
06-02-2020

Pack Size: 20 Plates / 100 Plates Storage Temperature: 15°C-25°C

Test Certificate No.: MX/FPPP/2020/0055-S Date Of Release: 08/06/2020

16-02-2020

Composition/Litre:

Description Quantity UOM

Xylose 3.5 g

L-Lysine 5.0 g

Lactose Monohydrate 7.5 g

Sucrose 7.5 g

Sodium Chloride 5.0 g

Yeast Extract 3.0 g

Phenol Red 0.08 g

Agar 13.5 g

Sodium Deoxycholate 2.5 g

Sodium Thiosulphate 6.8 g

Ferric Ammonium Citrate 0.8 g

*Formula adjusted to suit performance parameters.

Note : The medium may exhibit slight precipitation, which is inherent property of the medium, and it has no impact on the product performance.

Testing Parameters:

Test Specification Results

Appearance Gel with smooth, even surface, without any cracks,bubbles and drying or shrinking of media. Complies

Colour of medium Red coloured. Complies

Quantity of medium 26 ± 2 g in 90 mm petriplate. 26.979 g

Plates do not have any microbial contamination observed after incubation at 30-35°C & 20-25°C
Preincubation Complies
for more than or equal to 5 days.

Final pH at 25°C ± 2°C 7.20 – 7.60 7.52

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 CERTIFICATE OF ANALYSIS

Growth Promotion Test :

Growth promotion was carried out in accordance with the harmonized method of USP/EP/JP and growth was observed after an incubation at 30-35°C
for 18-48 hours.

Growth Promoting Properties :

Growth of the microorganisms is comparable to the previously tested and approved lot.
The test results observed are within the specified temperature and shortest period of time, inoculating ≤100 cfu of appropriate microorganism.

Indicative Properties :
Colonies are comparable in appearance and indication reaction to the previously tested and approved lot. The test results observed are within the
specified temperature and time, inoculating ≤ 100 cfu of appropriate microorganism.

Growth Promoting + Indicative

Organism Type Culture Growth Colour of Colony Incubation Temperature Incubation Period

Salmonella enterica subsp. enterica serovar

ATCC (14028) Good Red with black centres 30-35°C 18 Hours
Typhimurium

Salmonella enterica subsp. enterica serovar Abony NCTC (6017) Good Red with black centres 30-35°C 18 Hours

Note :
For Good Growth - Growth obtained on test media should not differ by a factor greater than 2 from calculated value for a standardized inoculum.

ATCC is a registered trade mark of the American Type Culture Collection.

NCTC (National Collection of Type Culture) is a registered trade mark of the Public Health England.

Microxpress dehydrated culture media incorporates raw materials and additives originating from BSE/TSE risk-free countries.

This is to certify that this lot passes and complies with the above mentioned tests and specifications.

Rolf Fernandes Chetalee Naik

Quality Control Quality Assurance
Date: 08/06/2020 Date: 08/06/2020

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