CERTIFICATE OF ANALYSIS

Product Name: R-2A Agar Plate (90 mm) (Triple Layer Pack, Gamma Irradiated )

Lot No.: P17-2001

P03-2016 Catalogue No.: 205180260100

Mfg.: 21-02-2019
03-06-2020
21-12-2019 Exp.: 20-06-2020
03-12-2020

Pack Size: 100 Plates Storage Temperature: 15°C-25°C

Test Certificate No.: MX/FPPP/2020/0159 Date Of Release: 31-12-2019

13/06/2020

Composition/Litre:

Description Quantity UOM

Yeast Extract 0.5 g

Proteose Peptone 0.5 g

Casein Hydrolysate 0.5 g

Glucose 0.5 g

Soluble Starch 0.5 g

Sodium Pyruvate 0.3 g

Dipotassium Phosphate 0.3 g

Magnesium Sulfate Anhydrous 0.024 g

Bacteriological Agar 15.0 g

* Formula adjusted to suit performance parameters.

Testing Parameters :

Test Specification Results

Appearance Gel with smooth and even surface without any cracks, bubbles and drying or shrinking of media. Complies

Colour of medium Light yellow coloured, slightly opalescent gel in petriplates. Complies

Quantity of medium 29 ± 2 g in 90 mm petriplate. 27.954 g

Plates do not have any microbial contamination after incubation at 30-35°C & 20-25°C for more
Preincubation Complies
than or equal to 5 days.

Final pH at 25°C ± 2°C 7.00 - 7.40 7.34

Gamma Irradiation : The above said product was Gamma Irradiated between 23KGy - 32KGy.
Growth Promotion Test :
Growth promotion was carried out in accordance with the harmonized method of USP/EP/JP and growth was observed after an incubation at 30-35°C
for ≤ 3 days for bacteria and at 30-35°C for ≤ 5 days for fungi.

Page 1 of 2
 CERTIFICATE OF ANALYSIS

Growth Promoting Properties :

Growth promoting properties are comparable to the previously tested and approved lot. The test results observed are within the specified
temperature and shortest period of time specified in the test, inoculating ≤ 100 cfu of appropriate microorganism.

Growth Promoting

Type Incubation Incubation

Organism Growth
Culture Temperature Period

Escherichia coli ATCC (8739) Good 30-35°C 24 Hours

Pseudomonas aeruginosa ATCC (9027) Good 30-35°C 24 Hours

Enterococcus faecalis ATCC (29212) Good 30-35°C 24 Hours

Staphylococcus aureus ATCC (6538) Good 30-35°C 24 Hours

Bacillus subtilis ATCC (6633) Good 30-35°C 24 Hours

Candida albicans ATCC (10231) Good 30-35°C 48 Hours

Aspergillus brasiliensis ATCC (16404) Good 30-35°C 72 Hours

Note:
For Good growth - growth obtained on test media should not differ by a factor greater than 2 from calculated value for a standardized inoculum.
Incase of water samples from fields it is suggested to incubate further upto 7 days to ascertain the absence of organisms.

ATCC is a registered trade mark of the American Type Culture Collection.

Microxpress dehydrated culture media incorporates raw materials and additives originating from BSE/TSE risk-free countries.

This is to certify that this lot passes and complies with the above mentioned tests and specifications.

Suchita Naik Chetalee Naik

Quality Control Quality Assurance
Date: 13/06/2020 Date: 13/06/2020

Page 2 of 2