Harlan Laboratories

Teklad Global Rodent Diets ®

Ingredient Comparison

Reducing Research Variables • Global Customer Relations and Service: We manage the
Teklad Global Diets® Ensure Reliable and Repeatable distribution and delivery to each customer to ensure global
Research Results availability and biosecurity and 100% customer satisfaction with
The Global line of Rodent Diets helps researchers worldwide reduce our diets for worldwide research.
research variables with:
• Quality Ingredients: Harlan guarantees strict and careful ingredient No Alfalfa Meal
selection with single source suppliers to ensure quality and
No Fish Meal
consistency.
No Animal Fat
• Diet Formulation: Global Rodent Diets contain levels of protein,
energy, vitamins, and minerals that more closely align with No Meat Meals
nutritional requirements in order to better meet the needs of No Meat By-Products
biomedical research studies. The fixed nature of these formulas, Minimal Cholesterol
along with careful ingredient selection and screening, provides for a
stable background on which to conduct research.
Lower Protein Levels
Zero or Reduced Soybean Meal
• Ingredient selection: Global Diets exclude ingredients that other
diets include. In the process, components that can affect research
results that are inherent in these ingredients of concern, such as
phytoestrogens, nitrosamines, cholesterol, mercury and other
contaminants, were also reduced or eliminated. We have reduced,
and in most cases eliminated, the set of ingredients of concern,
highlighted in red on the next pages.
• Quality Control Standards: Harlan has over 25 quality checks to Teklad Global Diets
control the manufacturing process. All Teklad operations and
quality processes are ISO9001-2000 Certified. Reducing Research Variables
The following chart shows ingredients of potential concern highlighted in red and how researchers worldwide can further reduce research variables.

Teklad Diets
Diet 2014 2016 2018 2019 2020X 7012 8604
Global 14% Global 16% Global 18% Global 19% Global18% LM-485 Teklad
Protein Protein Protein Protein Protein Sterilizable Rodent
Rodent Rodent Rodent Extruded Extruded Rodent Diet
Diet Diet Diet Rodent Rodent Diet
Diet Diet
Ingredients Wheat Midds Ground Ground Ground Ground Ground Corn Soybean
Wheat Wheat Wheat Wheat Meal
Ground Ground Corn Ground Corn Ground Corn Ground Corn Soybean Wheat Midds
Wheat Meal
Ground Corn Wheat Midds Wheat Midds Corn Gluten Corn Gluten Ground Oats Flaked Corn
Meal Meal
Corn Gluten Corn Gluten Soybean Meal Wheat Midds Wheat Midds Wheat Midds Ground Corn
Meal Meal
Brewers Yeast Corn Gluten Brewers Yeast Brewers Yeast Alfalfa Meal Fish Meal
Meal
Brewers Yeast Corn Gluten Molasses
Meal
Brewers Ground
Yeast Wheat
Isoflavone nd - 20 mg/kg nd - 20 mg/kg 150-250 mg/kg nd - 20 mg/kg nd - 20 mg/kg 300-500 mg/kg 350-575 mg/kg
Content✝
Protein 14.0% 16.0% 18.0% 19.0% 18.0% 19.0% 24.0%

Fat 4.0% 4.0% 6.0% 9.0% 6.0% 5.5% 4.5%

Source of Soybean Oil* Soybean Oil* Soybean Oil* Soybean Oil* Soybean Oil* Soybean Oil* Soybean Oil*
Added Fat

Metabolizable 2.9 kcal/g 3.0 kcal/g 3.1 kcal/g 3.4 kcal/g 3.1 kcal/g 3.0 kcal/g 3.0 kcal/g
Energy

✝
Expected range of daidzein plus genistein based on routine monitoring of diet and/or soybean meal source. nd = non-detectable
* Unlike Soybean Meal, Soybean Oil does not contain phytoestrogens.

All Teklad Diets are Fixed Formula diets. All Teklad Global Rodent Diet formulations are also available in autoclavable, irradiated &
certified formats. For example, for 2018:
2018 Non-Autoclavable Form
2018S Autoclavable* Form (Steam Sterilizable)
2918.15 Irradiated Form, in 15 Kg. Poly Cap Sacks
2918.CS Irradiated Form, in cases of 5 x 5 Lb. Vacuum Packs
2018C Certified Form
2018SX Autoclavable* & Extruded Form
The autoclavable version differs only in the level of vitamin supplementation, which is increased to account for presumed losses due to autoclaving.
 Other Diets
Diet 5001 5002 5008 5010 5P00 5P14 5053 5058
PMI PMI PMI PMI Auto RMH RMH PMI PMI
Rodent Certified Formulab Rodent 3000 2500 Irradiated Irradiated
Rodent Rodent Rodent Rodent

Ingredients Ground Ground Ground Ground Ground Soybean Ground Ground

Corn Corn Corn Corn Wheat Meal Corn Wheat
Soybean Soybean Soybean Soybean Soybean Ground Soybean Ground
Meal Meal Meal Meal Meal Corn Meal Corn
Beet Pulp Ground Ground Wheat Wheat Wheat Wheat Soybean
Wheat Wheat Midds Midds Midds Midds Meal
Fish Meal Fish Meal Fish Meal Fish Meal Ground Molasses Ground Wheat
Corn Wheat Germ
Ground Wheat Wheat Ground Fish Meal Ground Fish Meal Fish Meal
Oats Midds Midds Wheat Oats
Brewers Brewers Animal Fat Wheat Animal Fat Alfalfa Molasses Brewers
Yeast Yeast Germ Meal Yeast
Molasses Molasses Molasses Brewers Alfalfa Fish Meal Wheat Corn Gluten
Yeast Meal Germ Meal
Also Contains: Meat Meal Alfalfa Meal Meat Meal Alfalfa Meal Meat Meal Alfalfa Meal

Alfalfa Meal Alfalfa Meal Soy Hulls

Protein 23.0% 20.0% 23.0% 23.0% 22.0% 23.0% 20.0% 20.0%

Fat 5.0% 5.0% 6.5% 5.0% 5.0% 4.5% 5.0% 9.0%

Source of Animal Fat Soybean Oil* Animal Fat Animal Fat Animal Fat Animal Fat Soybean Oil* Animal Fat
Added Fat
Soybean Oil* Soybean Oil* Soybean Oil*

Metabolizable 3.0 kcal/g 3.1 kcal/g 3.3 kcal/g 3.1 kcal/g 3.2 kcal/g 3.0 kcal/g 3.1 kcal/g 3.5 kcal/g
Energy
Ingredients are listed top to bottom in decreasing order of prevalence in the diet.
Ingredient listings and nutrient information for PMI diets as per website, July 2009
PMI diets are produced using Constant Nutrition®, a variable formula process where ingredient inclusion rates are subject to change without notice.

Teklad Global Diets mean consistency across all possible applications:

Non-Autoclavable Certified Autoclavable Irradiated Consistency Factor
Teklad Rodent 2018 2018C 2018S or 2018SX 2918 Teklad - Identical
Diet Options Macro ingredient
formulations

PMI Rodent Diet 5001 5002 5010 5053 PMI - Requires the
Options use of 4 completely
different diets
 Phytoestrogens
Phytoestrogens are naturally occurring chemicals in plants which Historical Phytoestrogen (daidzein & genistein)
interact with endogenous estrogens and can potentially affect Content of Soybean Meal
research results. Soybean and alfalfa meal are the major
sources of phytoestrogens in laboratory rodent diets. When creating

2500
2490
2380
the Teklad Global Rodent Diets, we reduced this research variable

2330
2500

2045

2164
2020
by totally eliminating alfalfa meal and reducing or eliminating

1949
1845

1830
1786

1649
2000

1764

1606
Phytoestrogen, ppm
soybean meal.

1579
1500
Research Potential Affected by Phytoestrogens 1000
• Reproductive and Endocrine Disruptor Studies
500
• Cancer Studies (Breast, Prostate, Colorectal, Lung)
• Cardiovascular Disorders (Thrombosis, Atherosclerosis) 0

Q1 ’05
Q2 ‘05
Q3 ’05
Q4 ’05
Q1 ’06
Q2 ’06
Q3 ’06
Q4 ’06
Q1 ‘07
Q2 ’07
Q3 ’07
Q4 ‘07
Q1 ‘08
Q2 ’08
Q3 ’08
• Lipid and Cholesterol Studies
• Endocrine Studies (Insulin, Vasopressin, Thyroid Hormones)
• Bone Metabolism Studies Period of Sourcing (Quarter and Year)
• Central Nervous System Studies (Behavior, Memory, Learning,
Neural Degeneration) The variable levels of phytoestrogen content in soybean
meal make research results using diets containing this
• Immunology
ingredient unreliable.
• Gene Expression Studies
• Diabetes
Nitrosamines
Animal products and by-products such as fish meal and meat and bone
meal often used in rodent diets may be preserved using
Impact of Dietary Genistein on Breast Cancer Cell Growth1 many chemicals, including sodium nitrite. Under certain conditions,
OVX ACCL MCF-Est. 22 weeks
nitrites can interact with secondary amines to form potent carcinogens
known as nitrosamines. Given the obvious risk to research, Harlan
21d 7d 7d 7d
formulated Teklad Global Rodent Diets to exclude all meat and meat
by-products, including fish meal, effectively eliminating nitrosamines as a
research variable (Global Rodent Diets routinely contain < 3 ppb).
Growth of MCF-7 cells implanted in athymic nude mice
180

160
c
Traditional Rodent Diets
140
c Animal Byproducts
120
Nitrosamines
Tumor Surface Area

Nitrites
100
b (preservatives)
80 +
60
Tumor Growth
Baseline -
b Secondary Amines Tumor Growth
38 square mm
40
a
20
a
0 Alfalfa Meal and Imaging
NC PC 125Ge 250Ge 500Ge 1000Ge The presence of chlorophyll in alfalfa meal
NC=AIN-93G // PC=E17b // AIN-93G+Genistein and possibly other pigments such as those
Tumor growthininmice
Tumor growth miceconsuming
comsuming various
various levels
levels of of
present in fish meal, can significantly
genistein atatweek
Genistein week22.
22. (Ge=Genistein in PPM) increase auto-fluorescence and obscure or
reduce the clarity of certain types of optical
1
Ju YH, Allred CD, Allred KF, Karko KL, Doerge DR, Helferich WG. 2001. Physiological concentrations
of dietary genistein dose-dependently stimulate growth of estrogen-dependent human breast
imaging scans. Natural ingredient diets
Diet with Alfalfa-Free
cancer (MCF-7) tumors implanted in athymic nude mice. J Nutr 131:2957-2962.
that exclude alfalfa meal and fish meal, Alfalfa Diet
such as all Teklad Global Rodent Diets, Images courtesy of Caliper Life Sciences
have low fluorescent signals, resulting in
higher quality images.

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are trademarks of Harlan Laboratories, Inc. RMS1008-US-EN-01-PS-05

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