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Nutritive value?
1. Age
2. Fibre contents ( NDF ADF)
3. Protein
4. Legume vs grasses
5. Leaf to stem ratio
6. Physical and chemical treatments
7. Anti Quality factors ( Lignin, Alkaloids, tannins, cutin and silica)
8. Fertilization
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Measure of protein quality for mono gastric animals
Protein quality
• Characteristics of a protein in relation to its ability to achieve
defined metabolic actions or
• Protein's ability to provide specific patterns of amino acids to
satisfy the demands for synthesis of protein or
• Usefulness of a dietary protein for growth and maintenance of
tissue, and, in animals, production of meat, eggs, wool, and
milk
• Protein quality also depends on bioavailability or digestibility of
a protein or
• the capacity to provide metabolically available nitrogen and
amino acid to tissues and organs
• All proteins are not equal
• Gelatin, Zein, Casein have different effects on growth
• What makes them different?
• Amino acids they are made up of
• Total amino acids: 21
• Essential amino acids: 9
• Non-essential amino acids: 12
Estimate of protein quality
(a) Biological assays
(b) Chemical analysis
(100− 20+0 )
𝐵𝑉 = x 100 = 100% or 1.0
(100−20)
(100− 20+10 )
𝐵𝑉 = x 100 = 88% or .88
(100−20)
If Urinary Nitrogen =20, then BV ?
(100− 20+20 )
𝐵𝑉 = x 100 = 75% or .75
(100−20)
(100− 20+80 )
𝐵𝑉 = x 100 = 0%
(100−20)
NPU = BV × digestibility
(100− 20+0 )
𝐵𝑉 = x 100 = 100% or 1
(100−20)
Example : Calves gain 1000 g/ day and their daily protein intake was 450 g/ day
Net Protein Retention (NPR) is the weight gain of animals fed the test protein,
minus the weight loss of a group fed a protein‐free diet, divided by the protein
consumed
Protein Retention Efficiency (PRE) is the NPR converted into a percentage scale
by multiplying by 16: it then becomes numerically the same as Net Protein
Utilization
Relative Protein Value (RPV) is the ability of a test protein, fed at
various levels of intake, to support nitrogen balance, relative to a
standard protein
Essential amino acid index is the sum of all the essential amino
acids compared with those in egg protein or the amino acid
target mixture
Protein Digestibility Corrected Amino Acid Score (PDCAAS) is a
method of evaluating the protein quality based on both the amino
acid requirements of humans and their ability to digest it
Reasons:
• Complicated ?