CONFIDENTIAL ASIDEC 2018/810462 UNIVERSITI TEKNOLOGI MARA FINAL EXAMINATION COURSE : BIOCHEMISTRY COURSE CODE BlO462 EXAMINATION : DECEMBER 2018 TIME 2 HOURS | INSTRUCTIONS TO CANDIDATES 1 This question paper consists of five (5) questions. 2 ‘Answer ALL questions in the Answer Booklet. Start each answer on a new page. 3 Do not bring any material into the examination room unless permission is given by the invigilator. 4 Please check to make sure that this examination pack consists of: i) the Question Paper ii) an Answer Booklet ~ provided by the Faculty 5. ‘Answer ALL questions in English. DO NOT TURN THIS PAGE UNTIL YOU ARE TOLD TO DO SO This examination paper consists of 4 printed pages (© Hak Cipta Universiti Teknologi MARA CONFIDENTIALCONFIDENTIAL 2 QUESTION 1 a) State the Laws of Thermodynamics. ASIDEC 2018/B10462 (3 marks) b) The change in enthalpy for a reaction is -10 kJ. mol". Determine this reaction in terms of nsfer. transfer. ¢) Calculate the pH of 1 L of water to which Is added i) 10 mL of 5.0 MHC! li) 10 mL of 5.0 MNaOH QUESTION 2 a) Name three examples of each polar and non-polar amino acids. b) State the region in an enzyme molecule where a substrate fits. c) Give three characteristics of an enzyme. d) Determine the differences between a synthase and a synthetase. @) Describe the purpose of urea production in humans. f) Explain the role of glutamate in transamination. g) Compare between fibrous and globular proteins. QUESTION 3 ) State one example of aldose sugar. b) Give the three control points in the glycolytic pathway. (© Hak Cipte UniverattiTeknologl MARA, (2 marks) (2 marks) (3 marks) (6 marks) (1 mark) (3 marks) (2marks) (1 mark) (5 marks) (4marks) (1 mark) (3 marks) CONFIDENTIAL,CONFIDENTIAL 3 ASIDEC 2018/B10462 c) Propose a reason why the Cori cycle takes place in the liver and muscles. (2 marks) d) Compare the energy used in the conversion of two moles of pyruvate to glucose with the energy generated during glycolysis when one mole of glucose is converted to two moles of pyruvate. (2 marks) @) Distinguish how the energy used for gluconeogenesis is obtained. (2 marks) f) Illustrate the gluconeogenesis pathway. (10 marks) QUESTION 4 a) Sketch the structure of a phosphoacylglycerol. (1 mark) b) State the processes and enzymes involved in each of the steps in 2-oxidation cycle. (8 marks) ¢) Elaborate on the carboxylation of acetyl-coA during the synthesis of a fatty acid, (3 marks) d) Determine how the composition of membrane bilayer affects its properties. (4 marks) (© Hak Cipta Universiti Toknolog! MARA CONFIDENTIAL,CONFIDENTIAL, 4 ASIDEC 2018/B10462 QUESTION 5 a) Identify four physical traits determined by DNA. (4 marks) b) Describe briefly the steps in DNA replication. (3 marks) ¢) Label all the proteins (m-q) involved in DNA replication as shown in Figure 1. (5 marks) END OF QUESTION PAPER © Hak Cipta Universiti Teknologi MARA. CONFIDENTIAL