Confidential Internal Sales Bulletin

Not for Distribution

This bulletin announces a new HORIBA Fluorescence Application Note (FL-2019-07-08) on Synchronous Scanning. It
is intended to inform our sales network about the inherent benefits of our technology, but the document itself is not
to be distributed outside of our sales network.

New HORIBA Life Science Application Note Based on Duetta Synchronous Scanning (FL-2019-07-08)

Synchronous scanning is a spectroscopic method designed to extract information from complex fluorescence
spectra that results from multiple fluorescing components. With a synchronous scan, both the excitation and the
emission monochromators are scanned at the same time, in synchrony, with a fixed offset wavelength value.

This technical note addresses how the Duetta Fluorescence and Absorbance Spectrometer performs a synchronous
scan. The synchronous fluorescence scan technique is used to extract component information from complex sample
mixtures, for example in oil analysis. While the synchronous scan method has been used for decades, a much better
method for fluorescence component analysis is to acquire a complete 3-D excitation and emission matrix (EEM). The
EEM captures much more information and can be used with chemometrics software for very precise, and complex,
component analysis.

This technical note compares both techniques, and provides comparison data and shows how a fluorometer like Duetta,
which quickly and easily measures EEMs, and also offers Absorbance-Transmission parameters and correction of the EEM
for the inner filter effect (A-TEEMTM), is the ideal tool for fluorescence component analysis.

HORIBA Sales Bulletins are confidential internal documents that are

intended to inform the HORIBA sales network of aspects of HORIBA This Sales Bulletin is internal and confidential.
instrumentation and use. They are not to be distributed outside of It is not for distribution outside the company.
HORIBA.