SCREENING OF POLYPHENOLIC CONTENTS AND ANTIOXIDANT PROPERTIES OF GARLIC

Siham OUROUADI, Abdelkarim KHIRAOUI, Aziz HASIB, Hamid MOUMENE, Naima ZAKI, Aziz OUATMANE, Abdel-Ali BOULLI

Laboratory of Environment and Valorisation of Agro ressouces; University Sultan Moulay Slimane. Faculty
of Sciences and Technology; Beni Mellal; Morocco

E-mail : siham.ourouadi@gmail.com

I- Abstract V- Determination of antioxidant activities

Garlic (Allium sativum) is one of the world’s oldest medicines and has been Antioxidant activities were studied using two methods: DPPH and ABTS radical
used not only for flavoring but also as a medicinal herb for its prophylactic and scavenging activity.
therapeutic properties. The aim of current research was to study and compare an
antiradical activity, phenolic, flavonoid and flavonol contents of garlic of five regions
in Morocco.
VI- Results
II- Materials and methods
0,250
The solvents and the chemicals used were of analytical grade, methanol was
used as solvent for extraction of antioxidants compounds.
Garlic bulbs (Allium sativum L.) were collected from deferent regions (Fez (Ain 0,200
Chgag), Meknes (Agouray), Ben Hmed, Tetouan (Bni Hssan), and Marrakesh (Ait
Ourir) flavonols
0,150
mg/g
Flavonoides
III- Geographic location mg/g
0,100 Phenols
mg/g

0,050

0,000
Fes Agouray Marrakech Ben hmed Bni hssan

Polyphenolic content of garlic in five areas of Morocco

62,00
DPPH radical scavening

60,00
58,00
activity %

56,00
54,00
52,00
50,00
48,00
46,00
Fez Meknes Marrakesh Ben Ahmed Tetouan

DPPH antiradical activity of garlic in five areas of Morocco

30,00
ABTS radical scavening

25,00
Maps shows the areas of sampling garlic
activity %

20,00

IV- Determination of polyphenolic content 15,00

The measurements were determined using spectrophotometric method. 10,00

The concentration of total phenolic compounds was calculated as gallic acid 5,00

(mg/g) equivalent (GAE) from the calibration curve.

0,00
Fes Meknes Marrakech Ben Ahmed tetouan
The flavonoids and flavonols content were calculated as quercetin (mg/g)
equivalent (QE) from the calibration curve.
ABTS antiradical activity of garlic in five areas of Morocco

VII- Conclusion

A large variability in polyphenolic contents was observed among the cultivars. The fives cultivar represent a good sources of natural antioxidants and they could be
considered as useful sources of materials for human health, and the results showed, in general, a good correlation could be found between antioxidant activity and
polyphenolic compounds.