Appendix Frequently Asked Questions of Pipettor

Appendix A
Needle Coordinates& Frequently Asked Questions
of Pipettor

Needle Coordinates .............................................................................................................. 2

1 Coordinates................................................................................................................... 3
1.1 Preparation for coordinate............................................................................... 3
1.2 Procedures of Coordinates ............................................................................. 5
1.3 Adjustment of reference position ................................................................... 6
1.4 Adjustment of pipetting needle ..................................................................... 9
1.4.1 Adjustment of< First Sampling> position ......................................................... 10
1.4.2 Adjustment of Second Sampling after Incubate ............................................. 11
1.4.3 Adjustment of Second Sampling after Wash .................................................. 12
1.5 Washing position adjust................................................................................. 15
1.6 Sample Reagent area position adjust ......................................................... 17
1.6.1Sample area position adjust ............................................................................... 18
1.6.2 Reagent area position Adjust ........................................................................... 19
1.7 Z-Dispense position adjust........................................................................... 21
1.8 Adjustment of the pipetting needle in Z- Start position ............................. 22

Frequently Asked Questions of Pipettor ...................................................................... 23

1.9 Pipettor cannot be coordinated ............................................................ 24

2.0 How to change the pipettor of M600 .................................................... 32
2.1 How to disassemble the out-arm belt of M600 ..................................... 39

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Needle Coordinates

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Appendix Frequently Asked Questions of Pipettor

1 Coordinates

After installment of the analyzer and computer, the pipettors’ plane

position and the maximum position should be coordinated at first.

1.1 Preparation for coordinate

Prepare reaction cuvette filled with 75μl of distilled water respectively

st th st
in the 1 and 6 vial, then put it in the incubator (usually put in the 1
slot)
st
Prepare a reaction cuvette filled with 600 of distilled water in the 1
vial

Prepare three tubes filled with100μl of distilled water respectively

Then placed them at the track#1, 1st and 8th position, track#2,8th
position in sample area.

Prepare a blank reagent kit with 7 vials, the 1st vial (the position of
magnetic particle) fill with 200μldistilled water, the 2nd vial (the
position of low calibrator) fill 200μldistilled water, the 4th vial (the
position of displacer) fill with 500μldistilled water and the 7th vial (the
position of diluter) fill with 1000μldistilled water. Then slide the
reagent kit into the track#1 of Reagent Area.

Double click the [Maglumi Service] icon on the desktop, entered password :snibe923,the [Maglumi
Service]Interface will be shown as below Fig 1.1-1

Fig. 1.1-1: [MAGLUMI Service]dialog

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Press <Initialize>button on the top center of the dialog to execute

initialization of the plane assemblies.

When the initialization finishes, press <Incubator>button to open the

[Incubator] dialog. Select in the [target position], click
icon, back to the [Maglumi Service]interface . Click

button to open [Sample Loader] dialog。

Fig. 1.1-2:[Sample Loader]dialog

Click <both> icon, one reaction module will be moved to the first
pipetting position
Click <OK> icon, back to the [Maglumi Service] dialog

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1.2 Procedures of Coordinates

Click the upper left corner button of [Maglumi 600

Service] interface to open[Needles Adjust]dialog.

Fig. 1.2-1: [Needles Adjust] dialog

After finishing needle arm initialize, the inner arm and outer arm should be on the same line with
around 45°degree(the pipettor arm direct to the top-right corner of the sample &reagent area). If not,
then follow the chapter 1.9 Pipettor cannot be coordinated (followed this chapter) to check
the needle arm.

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Notices for Needle Coordination

During coordination, the Right-Way method arm adjustment only occurs in right Ref
position (Motion trace 2, as the figure 1 showed below). And rest arm adjustment
base on the Left-Way method (as the figure 2 showed below).

(1) (2)

1.3 Adjustment of reference position

Click button on upper right corner of the [Needles

Adjust] interface to open [Ref. Adjust]dialog.

Fig. 1.3-1: [Ref. Adjust] dialog

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Requirement:
1.Needletip should be located in the center point of the reference position, which is located on
the protection board of reagent area.

Reference Position

2.The Needletip higher than the reference position center point by 0.5mm

The coordinate detail procedures are below.

(1)Select<Motion Track1>In the field of [Select Motion

Track ]of reference position .

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Adjust the needle in X-axis and Y-axis direction with the 、

icon of Arm Outside & Arm inside, making the needletip just
above the target position

Adjust the needletip just above the center point of reference

position, Then press and icon to make the needletip
higher than the reference position center point by 0.5mm. Save the

parameters with icon, then press <OK> to exit.

(2)And then adjust the Motion Track2, the two arm should follow the
right-way method as the following picture shown.After adjust, save

the parameters with icon,and thenclick to

exit the interface.

Ensure that Motion Track1and Motion Track2

adjust the same reference position.

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1.4 Adjustment of pipetting needle

Preparea reaction cuvette filled with 75μl of distilled water

st th
respectively in the 1 and 6 vial, then move the cuvvete into
the first sampling area by software manually. Click
the icon (marked 1) in the right side of [Needles
Adjust] interface to open the [RightPipetting Position Adjust]
dialog

Fig. 1.4-1: [Right Pipetting Position Adjust]dialog

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1.4.1 Adjustment of< First Sampling> position

Select First Sampling in the field of [Select Sampling Time]

Adjust the Position of 1 & 6 in the field of [Select Area].

Fig. 1.4.1: [Right Pipetting Position Adjust] (First Sampling, Position of 1 & 6)

Adjust the needle in X-axis and Y-axis direction with the 、 icon of
Arm Outside & Arm inside, making the needletip just above the target

position .Then press and icon to adjust the pipettor needle. When
needle tip reach the liquid level, the LLD' light would be on showed in the

lower left corner of the message box, then press icon to save the
parameters.

Requirements:
1. Plane position: The edge of the needle should be aligned
with the corner of the reaction module cavity.

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2. Z-max in module: click and icon to adjust the pipettor

needle. When the needle tip reaches the liquid level, the LLD’ light

would be on, then press icon to save the parameters.

1.4.2Adjustment of Second Sampling after Incubate

* Move the cuvette to the first sampling position by software (use the sample loader)

Select Second Sampling after Incubate in the field of

[Select Sampling Time]
Adjust the Position of 1 & 6 in the field of [Select Area]

Fig. 1.4.2: [Right Pipetting Position Adjust]( Second Sampling after Incubate, Position of 1 & 6 )

Adjust the needle in X-axis and Y-axis direction with the 、

icon of Arm Outside & Arm inside, making the needletip just

above the target position .Then press and icon to adjust the
pipettor needle. When needle tip reach the liquid level, the LLD' light
would be on showed in the lower left corner of the message box ,

then press icon to save the parameters.

Requirements:
1. Plane position: The edge of the needle should be aligned with the
corner of the reaction module cavity.

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2. Z-max in module: click and icon to adjust the pipettor

needle. When the needle tip reaches the liquid level, the LLD’ light

would be on, then press icon to save the parameters.

1.4.3 Adjustment of Second Sampling after Wash

* Move the cuvette to the Second Sampling after wash by software (use the back transport)
manually.
Select Second Sampling after Wash in [Select Sampling Time] interface
Adjust the Position 1 & 6 & Mix in [Select Area]

Fig. 1.4.3-1: [Right Pipetting Position Adjust](Second Sampling after Wash, Position 1 & 6 )

Adjust the needle in X-axis and Y-axis direction with the 、

icon of Arm Outside & Arm inside, making the needletip just

above the target position .Then press and icon to adjust the
pipettor needle. When needle tip reach the liquid level, the LLD' light
would be on showed in the lower left corner of the message box ,

then press icon to save the parameters.

Requirements:
1. Plane: The edge of the needle should be approximately1-2 mm
away from the back wall& centered to the forward corner.

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2. Z-max in module: click to open to open 、 icon to

adjust the pipettor needle. When the needle tip reaches the
liquid level, the LLD’ light would be on, then press

icon to save the parameters.

3. When finish take out the cuvette

Select Position of Mix in [Select Area]

Fig. 1.4.3-2: [Right Pipetting Position Adjust](Second Sampling after Wash, Position ofMix )
A
d
j
u
s
t

t
h
e

n
e
e
d
l
e

i
n

X
-
a

xis and Y-axis direction with the 、 icon of Arm Outside &
Arm inside, making the needletip just above the target position .Then

press , the LLD' light would be on showed in the lower left

corner of the message box , then press icon to save the

parameters.

Requirements:
1.The needle should be centered to the forward corner.

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2. When you finish the coordinate in sampling area, take out the
cuvette

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1.5 Washing position adjust

Click the washing position icon in the [Needles Adjust] dialog

to open the [Washing Position Adjust] dialog.

Fig.1.5 : [Washing Position Adjust]dialog

Select [Waste Position] or [Prime Position] in [Select Area],

Adjust the needle in-X-axis and Y-axis direction with the 、

icon of Arm Outside & Arm inside, making the needletip just

above the target position .Then press and icon to adjust the

pipettor needle to the required position, then press icon to

save the parameters.

Requirements:

1. The needle of the waste position must always be higher than the
prime position wall. If the waste position is lower, the needle may
crash when moving from the waste to the prime position.
2. The needle of the Prime Position should be taught to the bottom
of the priming well and 3steps up.

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1.6 Sample Reagent area position adjust

Click the icon in the [Needles Adjust] dialog to open

the [Sample Area Reagent Adjust] dialog

Fig. 1.6-1: [Sample Reagent Area Adjust]diallog

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1.6.1Sample area position adjust

Select Position of Track 1 and Tube 1 & 8, Track 2 and Tube 8 in

[SelectSample Area]

Fig. 1.6.1: [Sample Reagent Area Adjust](Track 1 and Tube 1 & 8 , Track 2 and Tube 8)

,
Put the tubes in these related position.

Adjust the needle in X-axis and Y-axis direction with the 、

icon of Arm Outside & Arm inside, making the needletip just

above the target position .Then press and icon to adjust the
pipettor needle. When needle tip reach the liquid level, the LLD' light
would be on showed in the lower left corner of the message box, then

press icon to save the parameters.

Requirements:
st
1.Three tubes, each with 100μl distilled water, are place in the 1 and
th st th nd
8 position of 1 track, the 8 of the 2 track of sample area

2.Plane position: needle’s position should be just at the center point

of the tube mouth.

3. Z max in module: click and icon to adjust the pipettor

needle. When the needle tip reaches the liquid level, the LLD’ light

would be on, then press icon to save the parameters.

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1.6.2 Reagent area position Adjust

Select Position of Track 1 and Tube 1 & 2, & 4 & 7, Track 4 and Tube 7
in [Select Reagent Area]

Fig. 1.6.2: [Sample Reagent Area Adjust](Track 1 and Tube 1 & 2, & 4 & 7, Track 4 and Tube 7)

Put the reagent kit in the related Track

Adjust the needle in X-axis and Y-axis direction with the 、

icon of Arm Outside & Arm inside, making the needletip just

above the target position .Then press and icon to adjust the
pipettor needle. When needle tip reach the liquid level, the LLD' light
would be on showed in the lower left corner of the message box ,

then press icon to save the parameters.

Requirements:

1.Prepare a blank reagent kit , the 1st vial (the position of magnetic
particle) fill with 200μl water, the 2nd vial (the position of low
calibrator) fill 200μl water, the 4th vial (the position of displacer) fill
with 500μl water and the 7th vial (the position of diluter) fill with
1000μl water (as the picture shown in the following page).

2.Plane position: needle’s position should be just at the center point

of the sealing flap.

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3. Z max in module: click and icon to adjust the pipettor

needle. When the needle tip reaches the liquid level, the LLD’ light

would be on, then click icon to save the parameters.

st
4. When the adjustment of the 1 Track finished, take out of the
reagent kit and then slide it into the 4th track of Reagent Area

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1.7 Z-Dispense position adjust

st
Prepare a cuvette filled600μl water in the 1 vial, use the software
loaded it into the first sampling position ( use the sample loader)

Fig .1.7： Select pipetting needle in [Z-Dispense Adjust] dialog

Click and icon to adjust the pipettor needle. When the

needle tip reaches the liquid level, the LLD’ light would be on, then

click icon to save the parameters.

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1.8 Adjustment of the pipetting needle in Z- Start position

Put the blank reagent kit in the 1st track of reagent area

Click icon to open [Z-StartAdjust] dialog

Select Needle in [Select Needle]

Fig .1.8： Select Pipetting Needle in [Z-Dispense Adjust] dialog

Click and icon to adjust the pipettor needle. When the

needle tip (about one third of the Teflon area) insert into the tube,

click icon to save the parameters.

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Frequently Asked Questions of

Pipettor

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Appendix Frequently Asked Questions of Pipettor

1.9 Pipettor cannot be coordinated

Normally, when the pipettor arm is initialized, it will direct to the top-right corner of the sample &
reagent area, and the outer arm and inner arm are in the same line.

However, sometimes the pipettor arm's position maybe changed during the transport, which makes
the pipettor arm cannot get to the right position during initializing. In this circumstance, please check
the pipettor arm carefully as the following steps.
1.9.1 Move the panel away and the pipettor arm cover. There are two screws under the inner arm to
fix the inner arm cover; you need to loosen the two screws before move the inner arm cover.

1.9.2 After move the arm cover and the panel, then check the arm belt, both the inner arm belt and
outer arm belt need to be checked. If the belt is loosened, please tighten it as following steps:
(1)For the outer arm belt, please first loose the two screws as it marked in the following picture, then
move the motor to the left side to make the belt tighten. At last fix the two screws.

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(2) For the inner belt, it is same as the outer arm belt. If it loose, please first loose the four screws,
and then move the motor to tighten the belt and fix the screws.

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1.9.3 After make sure the two belts are tighten, please log in the service software, click [Coordinate],
the pipettor will initialize. After it initialized, please check whether the outer arm and inner arm in same
line, and the two arm should directing the top-right corner of the reagent area. You can refer the
following picture.

(1) If the inner arm is not directing to the top-right corner of the reagent area when you initialize the
pipettor.

Firstly, initialize the pipettor (click [Coordinate]), after initialize, please do not turn off the analyzer, and
do not quit the coordinate. Just loose the two screws marked A and B in the above picture, and then
move the inner arm to make it directing the top-right corner of the reagent area. Then tighten the two
screws.
(2) If the inner arm is directing to the top-right corner of the reagent area when you initialize the
pipettor, but the outer arm is not in the same line with the inner arm.

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Firstly, initialize the pipettor and do not turn off the analyzer and also do not quit the coordinate to
leave the pipettor maintaining initialize status. There are two screws in the wheel gear of the outer
arm. Loose the two screws as the above picture indicated, then move the outer arm to make it in the
same line with the inner arm. At last, tighten the two screws and initialize the pipettor to check
whether the pipettor is good.
1.9.4 After step 1 and step 2, if the pipettor is no problem when initialized. Then you can log in the
[coordinate], and adjust the needle position. Before you adjust the needle position, make sure that the
needle sensitivity (Coordinate>>>Inject) value is between 20~30, if the value is not in the range, you
can fill 25 in the area and then click [writeparam].

1.9.5 After the above steps, if the pipettor cannot detect the liquid level when you adjust the needle
position, or the needle cannot get to the right position after you finish the coordinate. Please check the
all cables of the pipettor, whether the cables were connected well. Especially the blue line of the
M600-02-E02 PCB in the inner arm.

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Appendix Frequently Asked Questions of Pipettor

B C

Check the cable of which connected to 02-E02 PCB (Fig A), 20-E01 PCB (Fig
B) and 02-E00 PCB -Sample& Reagent (Fig C).

1.9.6 If the cables are connected well, please check the 02-E02 PCB as the following instructions:
Use a multi-meter (short circuit function) to detect if there is short connection (circuit) for the capacitor.

Note: There are all 8 capacitors (C1,C2,C3,C4,C5,C6,C7,C8) on this PCB(M600-02-E02), which have
to be checked overall. If there is any capacitor is detected to be “short circuit” connection, it means
that capacitor is defective. Please report it to SNIBE engineer to ask for solution.

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If any of the capacity is detected to be “short circuit” connection, please contact to Snibe's
engineer.

After the above steps, generally, the analyzer will be normal. If the pipettor cannot work still, please
contact us.

After everything is normal, then please adjust the needle in the coordinate. For the Ref.Pos, please
make sure that the angle of inner arm and outer arm are different in the Motion Trace1 and Motion
trace 2. For other positions, please refer the <User manual>.

(1) For the [Ref.Pos], you must be careful that the Motion Trace 1 and Motion Trace 2 are different.

For motion trace 1, the inner arm and the outer arm angle like Fig 1.

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Fig 1

For motion trace 2, the inner arm and the outer arm angle like Fig 2.

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Fig 2
For other positions in the coordinate, please follow the chapter of needle coordinate.

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2.0 How to change the pipettor of M600

2.0.1 Loosen the screw of level panel, vertical panel, left panel, front cover and back panel.

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2.0.2 For the front panel, there are four screws in total please loosen them, disconnect the cable and
take the cover away.

2.0.3 Loosen the two screws, and take the holder out.

2.0.4 Loosen the two screws at the bottom of the pipettor, dismantle the two cable and the tube joint,
and then take the whole pipettor out.

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2.0.5 Install the new pipettor, following the other way as step 4.

2.0.6 Disassemble the pipettor needle from the old pipettor, and assemble it in the new pipettor.

(1). Please move the outer arm cover away (Fig 1).

Fig 1
(2). Loosen the screws and the black screw which connects the tube to the pipettor needle and
move it away; loosen the hexagon screw and move the black cable; disconnect the blue line
from the small board (Fig 2).

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Appendix Frequently Asked Questions of Pipettor

Fig 2

(3). Loosen the screws marked 1 and 2, and take the pin marked 3 out. Then take the whole
pipettor needle out of the outer arm, and take the pin out. (Fig 3&4)

(4). Now we dismantle the old pipettor needle, and get one old needle and five accessories (Fig
5).

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Fig 5
(5). Take the needle holder into the outer arm, make sure that the plane of the holder should
on the right side (Fig 6).

Fig 6

(6). Insert the new needle into the needle holder, install the spring and pin, and fix the needle with
the two screws.

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(7). Fix the trigger A and the black line. Make sure that the trigger A should right above the trigger
B (Fig 7).

Fig 7

(8). Connect the blue line to the small board, and rotate the black screw (on the tube) to the
pipettor needle (Fig 8)

Fig 8

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(9). Install the outer arm cover (Fig 9).

Fig 9

2.0.7 Install the front cover and all the panels as the other way of the above steps.

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2.1 How to disassemble the out-arm belt of M600

I. This technical guidance designed to teach you how to disassemble the out-arm belt of M600（See
the picture below）.

II. In order to disassemble the belt, we need to remove the front side panel 1 (See the pic below),

as well as the RFID reader cable, and dis-connect the white cable.

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III. Then, unscrew the two screws to remove the motor,

And disconnect the white cable,

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V. Next step, unscrew the two screws.

Now the belt can smoothly go through the motor and cables.

V. Unscrew the two screws, disconnect the 3 white cables,

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Then, dis-connect the black tube joint.

VI. When you finish the above steps, the belt can be smoothly take off from the analyzer.

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