GEN SINTETICO DE FASCIOLA

GenBank Description Protein Synthesised peptide Position Immune response

length (aa) sequence / Epitope
AAB02579.1 Amoebapore homologue 102 KGAGSSQDACIKFIQYEVDG / B1 63-82 Th1/Th2
AAB02579.1 Amoebapore homologue 102 KGAGSSQDATIKFIQYEVDG / B2 63-82 Th10
ABU62951.1 Cathepsin B 337 ISEIRDQSSTSSTWAVSSAS / B5 102-121 Th1/Th2/Th10/Th17
ABU62951.1 Cathepsin B 337 GVENGVKYWLIANSWNEGWG / 293-312 Th2
B6
AAB02579.1 Amoebapore homologue 102 TVNLVKRLLQNSVVE / T14 37-51 Th2
AAF88069.1 Amoebapore-like protein 101 DYIIDHVDQHNATEI / T15 80-94 Th1
ABU62925.1 Cathepsin B 337 DRNTQRQTVRYSVSE / T16 69-83 Th1/Th2

Rojas-Caraballo, J., López-Abán, J., Moreno-Pérez, D.A. et al. Transcriptome profiling of gene expression during immunisation trial
against Fasciola hepatica: identification of genes and pathways involved in conferring immunoprotection in a murine model. BMC
Infect Dis 17, 94 (2017). https://doi.org/10.1186/s12879-017-2205-3

amoebapore homolog [Fasciola hepatica]

GenBank: AAB02579.1
GenPept Identical Proteins Graphics
>AAB02579.1 amoebapore homolog [Fasciola hepatica]
MNPLFVLVLAAVALASTGVESEEPHLDISLCESCTNTVNLVKRLLQNSVVETHIRYLVKYLCKGAGSSQDACIKFIQYEVDGAVGYLIQHNATDICHVIRLC

amoebapore-like protein [Fasciola hepatica]

GenBank: AAF88069.1
>AAF88069.1 amoebapore-like protein [Fasciola hepatica]
MNPLFVLMLAAVTFASFDVPSKQPTIDIDLCDICTNTMDVIKKMLADQTVEEHIGYLVKYLCKIARSQDACIEFVQQEVDYIIDHVDQHNATEICRLIKLC
cathepsin B [Fasciola hepatica]
GenBank: ABU62925.1
>ABU62925.1 cathepsin B [Fasciola hepatica]
MSWLLIFAAIVVAQAKPNYKRQFEPFSDELIHYINEESGASWKAAPSTRFNNIDQVKQNLGVLEETPEDRNTQRQTVRYSVSENDLPESFDARQKWANCPSISEIRDQ
SSCSSCWAVSSASAITDRICIHSNGQKKPRLSAIDIVSCCAYCGYGCNGGIPAMSWDYWTREGVVTGGTLENPTGCLPYPFPKCSHGVVTPGLPPCPRDIYPTPKCEK
KCHAGYNKTYEQDKVKGKSSYNVGGQETDIMMEIMKNGPVDGIFYMFEDFLVYKSGIYHYTTGRLVGGHAIRVIGWGVENGVKYWLIANSWNEGWGEKGYFRMRRGNN
ECGIEARINAGLP

Table 1
Selected F. hepatica proteins containing signal peptide (SP) but no transmembrane domain (TM).

Epitop Synthesised peptide sequence Position GenBank Description Proteinlength (aa)

e

T14 DYIIDHVDQHNATEI 80–94 AAF88069.1 Amoebapore-like protein 101

T15 DRNTQRQTVRYSVSE 69–83 ABU62925.1 Cathepsin B 337

 Epitop Synthesised peptide sequence Position GenBank Description Proteinlength (aa)
e

T16 FYMFEDFLVYKSGIY 260–274 ABU62925.1 Cathepsin B 337

Rojas-Caraballo J, López-Abán J, Pérez del Villar L, et al. In vitro and in vivo studies for assessing the immune response and protection-inducing
ability conferred by Fasciola hepatica-derived synthetic peptides containing B- and T-cell epitopes. PLoS One. 2014;9(8):e105323. Published 2014
Aug 14. doi:10.1371/journal.pone.0105323