Za Rin Eng

How to use the microscopes 1.Setting up the microscope 2. Using the microscope 3.troubleshooting
hints ,and 4.clean up .

Setting up the microscope

1.Carring the microscope with holding the arm and under the base of the instrument .

2.Unwind the cord from the back of the microscope and plug it in. you can see On /off switch on the
back of the scope

3. check the ocular lenses and turn them until the number 64 lines up the white line below the rotating
part of the lens..Check that the condenser lens is just below the stage .

4.Put the objective lens on low poser and move the stage to its highest position. Adjust the light.

Using the microscope

1 Open the clip on the stage with one hand and slide a microscope slide to the back of the stage and
make sure the slide doesn’t go under the clips that hold in place .

2.Use the knobs on the lower right side of the stage to center the specimen over the condenser lens.

3. Start with the low power objective lens (4x)and the stage in its highest position .then Move to the
next highest objective lens (10x) and refocuses and last to the highest power lens .

Troubleshooting hints

If you are having difficulty focusing on the specimen.

1. Place you specimen in center .Start over at the lowest power and move the stage back to its
highest position .
2. When focusing , move the slide just a little bit back and forth with the slide holder knobs .if you
are observing unstained specimens ,use the stage diaphragm to reduce the amount of light
passing through, try to find a bubble or other easily observed object under the cover slip and
focus on that .
3. If when you move to higher poser lens, the specimen becomes blurry as though it were coated
with Vaseline and you cannot focus, you may need to clean the lens. Soak a piece of lens paper
with lens cleaner and wipe the lens. Then wipe dry with clean lens paper. Repeat a couple of
times. If this doesn’t solve the problem, check with your instructor.

Clean up

1. After you finished using remove the slide ,and rotate the lowest power lens in place .
2. Move the stage to a centered position and slide holder to the left.
3. Check that the stage is clean and dry.
4. Turn off the light and power and wrap the cord.

The Video so simple and clear easy to understand. The video show step by step on how to
use the microscope .