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Microscope
Care
Parts
Focusing
Light Microscope - uses compound (2) lenses to magnify
objects. The lenses bend or refract light to make the
object beneath them appear closer.
Eyepiece
Body Tube
Revolving Nosepiece
Arm
Objective Lens
Stage
Stage Clips
Coarse Focus
Diaphragm
Fine Focus
Light
Base
Magnification
Ocular Lens 10
multiply
General Procedures
1. Always start with the Scanning Objective.
2. Place your specimen in the center of your field of view.
3. Use the Coarse Knob to focus.
4. Next, use the Fine knob until clear.
5. Once you've focused on Scanning, switch to Low Power.
6. Repeat steps 3-4.
7. Now, switch to High Power.
8. At this point, ONLY use the ____
Fine ___________
Adjustment _____
Knob to
focus specimens.
Recap
1. Scanning --> use coarse and fine knob DO NOT SKIP
2. Low power --> use coarse and fine knob STEPS!!!!
3. High power --> use fine knob only
What will happen if I use the course knob under high power?
The slide or lens could crack
Field of View
view through a
Field of View: the area a person can _____
microscope.
circle
It is represented by a _______.
RELATIONSHIP: As magnification __________,
increases field
decreases
of view __________.
As you switch from scanning (40X) to high power
(100X), the area you see through the microscope gets
smaller.
We can see better detail with higher powers of
magnification, but we cannot see as much of the image.
The lens inverts the image of the object inside the
Inverted means that the image appears
microscope. _________
upside down and backward compared with the actual
object.
2. There's a spot in my viewing field, even when I move the slide the spot stays in
the same place!
Your lens is dirty. Use lens paper, and only lens paper to carefully clean the
objective and ocular lens. The ocular lens can be removed to clean the inside.
The spot is probably a spec of dust.
4. Only half of my viewing field is lit, it looks like there's a half-moon in there!
You probably don't have your objective fully clicked into place. .
Practice Labeling the Parts
Quiz Over the Microscope
1. When focusing a specimen, you should always start with the
___________________ objective.