GROUP 3

Jupiter, Larisa

EXPERIMENT 2

ADSORPTION OF A DYE ON A SILK CLOTHE

PROCEDURE

I. Calibration Curve

Acetic Acid

Assure pH = 3.00

100 mL in 100 mL in 100 mL in 100 mL in 100 mL in 100 mL in

E. flask E. flask E. flask E. flask E. flask E. flask

1 g juice 0.8 g juice 0.6 g juice 0.4 g juice 0.2 g juice 0.05 g juice
powder powder powder powder powder powder

Prepare blank solution (Acetic Acid + Water)

Determine λmax using blank and solution
Read absorbance at λmax starting with the lowest
concentration
Plot absorbance against concentration
 II. Adsorption

Prepared Solutions

Read absorbance at λmax and record as λ at t=0

Immerse 0.50 g of silk cloth to the dye solution
Read absorbance at different time intervals
0-10 min: 1-min interval
10-30 min: 5-min interval
30-90 min: 10-min interval
Determine amount of dye adsorb using a mass-balance
relationship
Plot qt (mg/g silk) against time (min)
Plot Langmuir and Freundlich adsorption isotherm

mass-balance relationship: Langmuir: Plot 1/ qe against 1/Ce

qt = (Co – Ct )
qe = (Co – Ce ) 𝑅𝐿 =
* Co – initial dye concentration; Ce – dye Freundlich: ln qee against ln Ce e
concentration wrt time; Ct – equilibrium ln qe = ln Qf + ln Ce
o
dye concentration
* V – volume of dye solutions (mL)
*W – weight of silk (g)