Examples of physical agents that are capable of denaturing proteins are heat, surface action,

ultraviolet light, ultrasound and many more. The examples of chemical agents that are capable
of denaturing proteins are acids, alkalis, heavy metal salts, urea, ethanol, guanidine detergents
and many more. There are type of bonds that are disrupted by these agents, like normal alpha-
helix and beta sheets in a protein, another example are the hydrogen bonds.

(n.d.). Retrieved from

http://chemistry.elmhurst.edu/vchembook/568denaturation.html#:~:text=Denaturation disrupts the
normal alpha,and tertiary structure are disrupted.

(n.d.). Retrieved from http://profilegenomics.com/merritt/protocol_PCR.html

https://www.biologydiscussion.com/proteins/denaturation-of-proteins-with-denaturing-agents/41902