AIM:

By the end of this lab experiment, I should be able to:

1.) Become familiar with yeast culture.

2.) Visualise and identify the unicellular Fungi.

INTRODUCTION

Bakers yeast is the common name for strains of yeast commonly used as a leavening
agent in baking bread and bakery products, where it converts the fermentable sugars
present in the dough into carbondioxide and ethanol. Bakers yeast is of the species
Saccharomyces cerevisiae, which is the same species (but a different strain) commonly
used in alcoholic fermentation, which is called Brewers yeast. Bakers yeast is also
single-cell microorganisms found on and around the human body.

Saccharomyces cerevisiae, is a unicellular fungus that reproduces by budding. During

the cell cycle,(while the replication machinery in the nucleus makes a second copy of
the genome), a small bud emerges from the Ovoid mother cell. This Buds continue to
grow as the cells prepares for cell division. During mitosis, the nucleus divides and one
full complement of DNA is packaged into the large bud before it is pinched off. Both the
mother and daughter cells go on to G1 where they grow and subsequently embark on a
new round of the cell cycle. Thus by observing the cellular morphology of S. Cerevisiae,
you can determine each cells position in the cell cycle.
 MATERIALS

-Yeast Extract, agar and dextrose.

-Petri dishes.
-70% Ethanol.
-Sterile Micropipette tips.
-Inoculation loops.
-Bakers Yeast.

METHOD
1.) Yeast Peptone Dextrose agar was prepared by adding 10g of Yeast extract in 20g
anhydrous dextrose and 20g agar with 1litre of distilled water.
2.) After preparation, the YPD was then sterilised at 121ºC for 15mins. The sterilised,
the media is then poured in the sterile petri dishes.
3.) After solidifications of agar, the yeast culture was spreaded over the surface of the
agar by spread plate method.
4.) After inoculation, the petri dish was then incubated at 30ºC for 24-48hrs.

Streaking
1.) A loopful full of yeast culture from the yeast broth was streaked from one end to
the other on the prepared YPD agar.

Observing Yeast Under Microscope.

1.) A cover slip was placed ontop of the sample by first resting one edge of the
coverslip on the slide adjacent to the droplet, and it was gently lowered to the
opposite edge of the cover slip.
2.) The slide was observed under lower resolution power than move upto high
resolution.
 RESULTS AND OBSERVATION
Diagram Colony Morphology
Streak 1

-Less Colony formation.

-Colony growth follow streaping pattern.

Streak 2 Streak 1: 8 colonies

Streak 2: 9 colonies
Fast growth.

Streak 3: 6 colonies

Colonies are all countable

Streak 3:
Spread Plate 1:

Spread plate 1: Colonies are spreadout

but can still be counted.

Spread Plate 2: Colony growth tend to fill

the whole peri dish
Spread Plate 2:
-Non-Countable

Spread plate 3: Petri dish is fully filled

with yeast cell growth.
1q
-Non-Countable

Spread Plate 3:
 CONCLUSION

To conclude, after performing this lab, I was able to understand the method and obtain
skills on how to cuture yeast cells. On the other hand, I was also able to visualise and
identify the unicellular fungi during our lab experiment. Streaking and Spread plate has
different growth pattern within desired time and temperature in an incubator as observed
as an evident in this lab. For streaking, colonies tends to be countable and less growth
seems to be occuring in the petri dish whereas Spread plate colonies are non-countable
and its growth fills up the petri dish.

REFERENCES
• https://kenanfellows.org/kfp-cp-sites/cp24/cp24/activity-3-lab-culturing-yeast-
cells-media/index.html
• Lab Handout for Bio 602, Practical 5
• https://www.google.com/search?
q=streaking+and+Spread+plate&oq=streaking+and+Spread+plate&aqs=chrome..
69i57.8177j0j7&sourceid=chrome&ie=UTF-8
•