University of Santo Tomas

College of Science
Department of Biological Sciences

WORKSHEET - EXERCISE 3
Aseptic Transfer of Culture and
Microbial Growth on Solid and Liquid Media
Name of student (Last name first) Date: ________________
Section: _____________
__________________________________________ Group No. __________

I. OBSERVATION: Verbalize any deviation/s from the proper techniques used in the
laboratory based on the descriptions given in the manual and compare with those
shown in the different video presentations. Give the scientific basis for the reasoning.

Video Missteps/ Unacceptable Scientific Contention

Presentation/Simulation Technique/Deviations/Improper
handling of microorganisms

1. dispensing of agar medium to

a Petri dish

2. Aseptic technique and transfer

of microbial cultures

3. Aseptic transfer from slant

culture to another sterile slant
medium)

4. Aseptic transfer using

Bactincinerator

5. Inoculation in different media

preparation

6. Detailed discussion of the

rationale for the cultivation of
microorganisms

7. Further discussion on culture

media classification and uses

8. Bacterial isolation using streak

plate technique

9. Time lapsed growth of a

bacterium

10. Colonial morphology and

growth patterns
 University of Santo Tomas
College of Science
Department of Biological Sciences

11. Bacterial colony description

I. DISCUSSION

A. Guide questions

1. How will you know if a culture medium is sterile prior to use?

2. What is the purpose of using the following preparations?

a. Agar deeps

b. Slants

c. Deep-slants

3. In a plated medium, what is the purpose of multiple streaking?

B. Extension question or case study for real-world application

Discuss the importance of microbial cultures in medical diagnostics and in industrial microbiology.
 University of Santo Tomas
College of Science
Department of Biological Sciences

III. REFERENCES (In APA format)