BIOINFORMATIKA

GEN ND4 PADA FAMILY ANATIDAE

DISUSUN OLEH

NAMA : KINANTI

NIM : G 401 16 021

JURUSAN BIOLOGI
FAKULTAS MATEMATIKA DAN ILMU PENGETAHUAN ALAMA
UNIVERSITAS TADULAKO
Gene : ND4

Gene type : Protein coding

Species : Anser fabalis

Genomic context
Sequence: Chromosome: MT; NC_016922.1 (10231..11609)

Chromosome MT - NC_016922.1

Genomic regions, transcripts, and products

Restriction Enzyme Map:
M L K I I L P T I I L L P T A L L S P P K F L W T N T
1 ATGCTAAAAATCATCCTACCCACAATCATACTTCTTCCAACAGCCCTGCTGTCTCCACCAAAATTTCTATGGACTAACAC 80
1 TACGATTTTTAGTAGGATGGGTGTTAGTATGAAGAAGGTTGTCGGGACGACAGAGGTGGTTTTAAAGATACCTGATTGTG 80
BstF5I MboII BsmAI XcmI
MmeI
ApoI

T L Y S L L I A A L S L Q * L I P T Y Y P Y K F L S
81 CACCTTATACAGCCTCCTAATCGCTGCCCTAAGCCTACAGTGATTAATCCCAACCTACTACCCCTACAAATTTTTATCCA 160
81 GTGGAATATGTCGGAGGATTAGCGACGGGATTCGGATGTCACTAATTAGGGTTGGATGATGGGGATGTTTAAAAATAGGT 160
BbvI MnlI Bpu10I TspRI ApoI
SfcI AseI

N * T G I D Q I S S P L L V L S C * L L P L I I I A S
161 ACTGAACAGGAATTGACCAAATCTCATCCCCCCTCCTAGTACTATCCTGCTGACTACTCCCACTCATAATCATAGCAAGC 240
161 TGACTTGTCCTTAACTGGTTTAGAGTAGGGGGGAGGATCATGATAGGACGACTGATGAGGGTGAGTATTAGTATCGTTCG 240
Hin4I MmeI Hin4I Cac8I
FokI BstF5I TatI
ScaI
MnlI

Q N H L Q Q E P L Q R K R I F I S T L V A V Q P F I L
241 CAAAACCACCTGCAACAAGAACCCCTACAACGCAAACGAATCTTTATCTCAACCTTAGTCGCAGTCCAGCCATTCATCCT 320
241 GTTTTGGTGGACGTTGTTCTTGGGGATGTTGCGTTTGCTTAGAAATAGAGTTGGAATCAGCGTCAGGTCGGTAAGTAGGA 320
AarI FokI BstF5I
BspMI TspDTI

L A F S T T E L A L F Y I S F E A T L I P T L I L I
321 ACTAGCCTTTTCCACCACAGAACTAGCACTATTTTACATCTCATTTGAAGCAACCCTCATTCCCACCCTAATCTTAATCA 400
321 TGATCGGAAAAGGTGGTGTCTTGATCGTGATAAAATGTAGAGTAAACTTCGTTGGGAGTAAGGGTGGGATTAGAATTAGT 400
MnlI
HphI

T R W G N Q P E R L S A G I Y L L F Y T P V S S L P L
401 CCCGATGGGGGAACCAACCAGAACGCCTAAGCGCTGGCATCTACCTGCTATTCTACACGCCAGTAAGCTCTCTACCCCTG 480
401 GGGCTACCCCCTTGGTTGGTCTTGCGGATTCGCGACCGTAGATGGACGATAAGATGTGCGGTCATTCGAGAGATGGGGAC 480
BslI Bpu10I Cac8I SfaNI BsrI
NlaIV AfeI BspMI
HaeII

L I T I I H L Y V K I G T L H L P T L Q L T H P T L S
481 CTAATCACAATCATACACCTATACGTGAAAATTGGCACCCTGCACCTGCCAACCCTACAACTAACCCACCCAACCCTATC 560
481 GATTAGTGTTAGTATGTGGATATGCACTTTTAACCGTGGGACGTGGACGGTTGGGATGTTGATTGGGTGGGTTGGGATAG 560
BsaAI BanI AarI
BsgI NlaIV BspMI

T S W T G I L S G L A L L I A F I V K A P L Y G L H
561 CACCTCATGGACAGGAATACTATCAGGTCTAGCACTGCTCATAGCATTCATAGTAAAAGCCCCACTATACGGCCTACACC 640
561 GTGGAGTACCTGTCCTTATGATAGTCCAGATCGTGACGAGTATCGTAAGTATCATTTTCGGGGTGATATGCCGGATGTGG 640
TaqII MnlI BtsI TspRI BslI
BslI TspDTI
PflMI BsmI
 L * L Q K A H V G D F V I D S I L L A A L L L K L G G
641 TCTGACTACAGAAAGCCCACGTAGGGGATTTCGTCATAGACTCAATACTCCTCGCCGCCCTACTACTAAAGCTAGGAGGA 720
641 AGACTGATGTCTTTCGGGTGCATCCCCTAAAGCAGTATCTGAGTTATGAGGAGCGGCGGGATGATGATTTCGATCCTCCT 720
BceAI BsaAI MlyI BseRI MnlI MnlI
SfcI BslI PleI BciVI
MnlI BslI

Y G I I R V T L F M G P L S N L L H Y P F L T L A L *
721 TACGGAATTATACGAGTCACTCTATTTATGGGCCCACTGTCCAACCTCCTGCACTACCCCTTCCTAACCCTGGCCCTATG 800
721 ATGCCTTAATATGCTCAGTGAGATAAATACCCGGGTGACAGGTTGGAGGACGTGATGGGGAAGGATTGGGACCGGGATAC 800
TspGWI PspOMI TspRI MnlI MmeI MnlI
PleI NlaIV BsaJI
MlyI ApaI BslI
BanII
Bme1580I
Bsp1286I
BsgI

G A L M T S S I C L R Q T D L K S L I A Y S S V S H
801 AGGCGCCCTAATGACTAGCTCAATCTGCCTACGACAGACAGACCTAAAATCACTAATCGCTTATTCATCCGTTAGCCACA 880
801 TCCGCGGGATTACTGATCGAGTTAGACGGATGCTGTCTGTCTGGATTTTAGTGATTAGCGAATAAGTAGGCAATCGGTGT 880
BanI MwoI Hin4I FokI TspGWI
KasI HpyF10VI TspDTI Hin4I
BsaHI Hin4I Hin4I
NarI BstF5I
NlaIV
SfoI
BbeI
HaeII

I G L V I A A G I I Q T H * S F S G A M I L I I S H G
881 TAGGCCTAGTTATCGCCGCAGGAATAATCCAAACCCACTGATCATTCTCAGGGGCAATGATCCTAATAATTTCCCATGGA 960
881 ATCCGGATCAATAGCGGCGTCCTTATTAGGTTTGGGTGACTAGTAAGAGTCCCCGTTACTAGGATTATTAAAGGGTACCT 960
BglI BclI AlwI BspCNI BsaJI
MwoI TspRI BseMII BtgI
HpyF10VI BsrDI NcoI
StuI StyI

L T S S I L F C L A N T N Y E R T H S R I L L L T R G
961 CTAACTTCCTCCATACTATTCTGCCTAGCCAACACAAACTATGAACGTACGCACAGCCGAATTCTACTACTCACACGAGG 1040
961 GATTGAAGGAGGTATGATAAGACGGATCGGTTGTGTTTGATACTTGCATGCGTGTCGGCTTAAGATGATGAGTGTGCTCC 1040
MnlI BsiWI TspDTI MnlI
ApoI BsaXI
EcoRI BssSI

L Q P L L P L I A T * * L L A N L T N I A L P P T T
1041 CCTACAACCTCTCCTACCACTCATAGCTACCTGATGATTGCTGGCCAATCTAACAAACATAGCCTTACCCCCAACAACAA 1120
1041 GGATGTTGGAGAGGATGGTGAGTATCGATGGACTACTAACGACCGGTTAGATTGTTTGTATCGGAATGGGGGTTGTTGTT 1120
StuI MnlI BsaXI Cac8I
EaeI
MscI

N L I A E L T I I I T L F N * S A F T I I L T G I A T
1121 ACCTCATAGCAGAGCTAACCATTATAATCACCTTATTCAACTGATCTGCCTTCACGATCATCCTAACAGGAATCGCAACC 1200
1121 TGGAGTATCGTCTCGATTGGTAATATTAGTGGAATAAGTTGACTAGACGGAAGTGCTAGTAGGATTGTCCTTAGCGTTGG 1200
MnlI HphI FokI Hpy188III
PsiI BstF5I
 L L T A S Y T L F I L L M T Q R G S I P S H I T S M Q
1201 CTACTAACCGCCTCCTACACCTTATTCATACTACTAATGACCCAACGAGGCTCAATCCCCTCCCACATCACATCTATGCA 1280
1201 GATGATTGGCGGAGGATGTGGAATAAGTATGATGATTACTGGGTTGCTCCGAGTTAGGGGAGGGTGTAGTGTAGATACGT 1280
TspDTI Hin4I MnlI
MnlI MnlI Hin4I
MslI

N S T T R E H L L I T L H I I P I F L L I L K P E L
1281 AAACTCAACCACGCGAGAACACCTGCTCATAACACTCCACATTATCCCAATATTCCTCCTAATCCTCAAACCCGAACTGA 1360
1281 TTTGAGTTGGTGCGCTCTTGTGGACGAGTATTGTGAGGTGTAATAGGGTTATAAGGAGGATTAGGAGTTTGGGCTTGACT 1360
PpiI AarI PpiI SspI MnlI MnlI
BspMI

I S G I P S
1361 TCTCCGGAATCCCCTCATA 1379
1361 AGAGGCCTTAGGGGAGTAT 1379
BsaWI
BspEI
Hpy188III

Restriction table:
Enzyme Recognition frequency Positions
__________________________________________________________________________
AarI CACCTGCnnnn'nnnn_ 3 258, 534, 1311
AfeI AGC'GCT 1 433
AlwI GGATCnnnn'n_ 1 934
ApaI G_GGCC'C 1 755
ApoI r'AATT_y 3 62, 149, 1020
AseI AT'TA_AT 1 125
BanI G'GyrC_C 2 515, 803
BanII G_rGCy'C 1 755
BbeI G_GCGC'C 1 807
BbvI GCAGCnnnnnnnn'nnnn_ 1 91
BceAI ACGGCnnnnnnnnnnnn'nn_ 1 646
BciVI GTATCCnnnnn_n' 1 713
BclI T'GATC_A 1 920
BglI GCCn_nnn'nGGC 1 882
Bme1580I G_kGCm'C 1 755
Bpu10I CC'TnA_GC 2 110, 428
BsaAI yAC'GTr 2 505, 661
BsaHI Gr'CG_yC 1 804
BsaJI C'CnnG_G 2 789, 955
BsaWI w'CCGG_w 1 1364
BsaXI ACnnnnnCTCCnnnnnnn_nnn' 1 1065
BsaXI GGAGnnnnnGTnnnnnnnnn_nnn' 1 1035
BseMII CTCAGnnnnnnnn_nn' 1 942
BseRI GAGGAGnnnnnnnn_nn' 1 680
BsgI GTGCAGnnnnnnnnnnnnnn_nn' 2 506, 755
BsiWI C'GTAC_G 1 1007
BslI CCnn_nnn'nnGG 6 408, 567, 629, 663, 664, 790
BsmI GAATG_Cn' 1 605
BsmAI GTCTCn'nnnn_ 1 57
Bsp1286I G_dGCh'C 1 755
BspCNI CTCAGnnnnnnn_nn' 1 941
BspEI T'CCGG_A 1 1364
BspMI ACCTGCnnnn'nnnn_ 4 258, 453, 534, 1311
BsrI ACTG_Gn' 1 461
BsrDI GCAATG_nn' 1 942
BssSI C'ACGA_G 1 1035
BstF5I GGATG_nn' 5 12, 185, 315, 866, 1179
BtgI C'CryG_G 1 955
BtsI GCAGTG_nn' 1 593
Cac8I GCn'nGC 3 238, 436, 1083
EaeI y'GGCC_r 1 1083
EcoRI G'AATT_C 1 1020
FokI GGATGnnnnnnnnn'nnnn_ 4 172, 302, 853, 1166
HaeII r_GCGC'y 2 435, 807
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' 4 199, 833, 865, 1239
Hin4I GAbnnnnnrTCnnnnnnnn_nnnnn' 4 167, 833, 865, 1271
HphI GGTGAnnnnnnn_n' 2 391, 1141
Hpy188III TC'nn_GA 2 1174, 1365
HpyF10VI GCn_nnnnn'nGC 2 826, 883
KasI G'GCGC_C 1 803
MboII GAAGAnnnnnnn_n' 1 26
MlyI GAGTCnnnnn' 2 674, 744
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' 3 62, 183, 786
MnlI CCTCnnnnnn_n' 18 104, 203, 386, 574, 650, 701
710, 776, 794, 979, 1031, 1059
1133, 1222, 1241, 1270, 1346
1355
MscI TGG'CCA 1 1085
MslI CAynn'nnrTG 1 1274
MwoI GCnn_nnn'nnGC 2 825, 882
NarI GG'CG_CC 1 804
NcoI C'CATG_G 1 955
NlaIV GGn'nCC 4 413, 517, 753, 805
PflMI CCAn_nnn'nTGG 1 567
PleI GAGTCnnnn'n_ 2 674, 743
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' 1 1322
PpiI GAGnnnnnGTTCnnnnnnn_nnnnn' 1 1290
PsiI TTA'TAA 1 1145
PspOMI G'GGCC_C 1 751
ScaI AGT'ACT 1 201
SfaNI GCATCnnnnn'nnnn_ 1 447
SfcI C'TryA_G 2 116, 647
SfoI GGC'GCC 1 805
SspI AAT'ATT 1 1332
StuI AGG'CCT 2 885, 1041
StyI C'CwwG_G 1 955
TaqII CACCCAnnnnnnnnn_nn' 1 564
TatI w'GTAC_w 1 199
TspDTI ATGAAnnnnnnnnn_nn' 5 304, 598, 855, 1017, 1216
TspGWI ACGGAnnnnnnnnn_nn' 2 738, 859
TspRI _nnCAsTGnn' 4 125, 600, 762, 923
XcmI CCAnnnn_n'nnnnTGG 1 66

Enzymes that cut five or fewer times

Enzyme Recognition frequency Positions

__________________________________________________________________________
AarI CACCTGCnnnn'nnnn_ 3 258, 534, 1311
AfeI AGC'GCT 1 433
AlwI GGATCnnnn'n_ 1 934
ApaI G_GGCC'C 1 755
ApoI r'AATT_y 3 62, 149, 1020
AseI AT'TA_AT 1 125
BanI G'GyrC_C 2 515, 803
BanII G_rGCy'C 1 755
BbeI G_GCGC'C 1 807
BbvI GCAGCnnnnnnnn'nnnn_ 1 91
BceAI ACGGCnnnnnnnnnnnn'nn_ 1 646
BciVI GTATCCnnnnn_n' 1 713
BclI T'GATC_A 1 920
BglI GCCn_nnn'nGGC 1 882
Bme1580I G_kGCm'C 1 755
Bpu10I CC'TnA_GC 2 110, 428
BsaAI yAC'GTr 2 505, 661
BsaHI Gr'CG_yC 1 804
BsaJI C'CnnG_G 2 789, 955
BsaWI w'CCGG_w 1 1364
BsaXI ACnnnnnCTCCnnnnnnn_nnn' 1 1065
BsaXI GGAGnnnnnGTnnnnnnnnn_nnn' 1 1035
BseMII CTCAGnnnnnnnn_nn' 1 942
BseRI GAGGAGnnnnnnnn_nn' 1 680
BsgI GTGCAGnnnnnnnnnnnnnn_nn' 2 506, 755
BsiWI C'GTAC_G 1 1007
BsmI GAATG_Cn' 1 605
BsmAI GTCTCn'nnnn_ 1 57
Bsp1286I G_dGCh'C 1 755
BspCNI CTCAGnnnnnnn_nn' 1 941
BspEI T'CCGG_A 1 1364
BspMI ACCTGCnnnn'nnnn_ 4 258, 453, 534, 1311
BsrI ACTG_Gn' 1 461
BsrDI GCAATG_nn' 1 942
BssSI C'ACGA_G 1 1035
BstF5I GGATG_nn' 5 12, 185, 315, 866, 1179
BtgI C'CryG_G 1 955
BtsI GCAGTG_nn' 1 593
Cac8I GCn'nGC 3 238, 436, 1083
EaeI y'GGCC_r 1 1083
EcoRI G'AATT_C 1 1020
FokI GGATGnnnnnnnnn'nnnn_ 4 172, 302, 853, 1166
HaeII r_GCGC'y 2 435, 807
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' 4 199, 833, 865, 1239
Hin4I GAbnnnnnrTCnnnnnnnn_nnnnn' 4 167, 833, 865, 1271
HphI GGTGAnnnnnnn_n' 2 391, 1141
Hpy188III TC'nn_GA 2 1174, 1365
HpyF10VI GCn_nnnnn'nGC 2 826, 883
KasI G'GCGC_C 1 803
MboII GAAGAnnnnnnn_n' 1 26
MlyI GAGTCnnnnn' 2 674, 744
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' 3 62, 183, 786
MscI TGG'CCA 1 1085
MslI CAynn'nnrTG 1 1274
MwoI GCnn_nnn'nnGC 2 825, 882
NarI GG'CG_CC 1 804
NcoI C'CATG_G 1 955
NlaIV GGn'nCC 4 413, 517, 753, 805
PflMI CCAn_nnn'nTGG 1 567
PleI GAGTCnnnn'n_ 2 674, 743
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' 1 1322
PpiI GAGnnnnnGTTCnnnnnnn_nnnnn' 1 1290
PsiI TTA'TAA 1 1145
PspOMI G'GGCC_C 1 751
ScaI AGT'ACT 1 201
SfaNI GCATCnnnnn'nnnn_ 1 447
SfcI C'TryA_G 2 116, 647
SfoI GGC'GCC 1 805
SspI AAT'ATT 1 1332
StuI AGG'CCT 2 885, 1041
StyI C'CwwG_G 1 955
TaqII CACCCAnnnnnnnnn_nn' 1 564
TatI w'GTAC_w 1 199
TspDTI ATGAAnnnnnnnnn_nn' 5 304, 598, 855, 1017, 1216
TspGWI ACGGAnnnnnnnnn_nn' 2 738, 859
TspRI _nnCAsTGnn' 4 125, 600, 762, 923
XcmI CCAnnnn_n'nnnnTGG 1 66

Position Enzyme(s)
__________________________________________________________________________
12 BstF5I GGATG_nn'
26 MboII GAAGAnnnnnnn_n'
57 BsmAI GTCTCn'nnnn_
62 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
62 ApoI r'AATT_y
66 XcmI CCAnnnn_n'nnnnTGG
91 BbvI GCAGCnnnnnnnn'nnnn_
104 MnlI CCTCnnnnnn_n'
110 Bpu10I CC'TnA_GC
116 SfcI C'TryA_G
125 TspRI _nnCAsTGnn'
125 AseI AT'TA_AT
149 ApoI r'AATT_y
167 Hin4I GAbnnnnnrTCnnnnnnnn_nnnnn'
172 FokI GGATGnnnnnnnnn'nnnn_
183 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
185 BstF5I GGATG_nn'
199 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
199 TatI w'GTAC_w
201 ScaI AGT'ACT
203 MnlI CCTCnnnnnn_n'
238 Cac8I GCn'nGC
258 AarI CACCTGCnnnn'nnnn_
258 BspMI ACCTGCnnnn'nnnn_
302 FokI GGATGnnnnnnnnn'nnnn_
304 TspDTI ATGAAnnnnnnnnn_nn'
315 BstF5I GGATG_nn'
386 MnlI CCTCnnnnnn_n'
391 HphI GGTGAnnnnnnn_n'
408 BslI CCnn_nnn'nnGG
413 NlaIV GGn'nCC
428 Bpu10I CC'TnA_GC
433 AfeI AGC'GCT
435 HaeII r_GCGC'y
436 Cac8I GCn'nGC
447 SfaNI GCATCnnnnn'nnnn_
453 BspMI ACCTGCnnnn'nnnn_
461 BsrI ACTG_Gn'
505 BsaAI yAC'GTr
506 BsgI GTGCAGnnnnnnnnnnnnnn_nn'
515 BanI G'GyrC_C
517 NlaIV GGn'nCC
534 AarI CACCTGCnnnn'nnnn_
534 BspMI ACCTGCnnnn'nnnn_
564 TaqII CACCCAnnnnnnnnn_nn'
567 BslI CCnn_nnn'nnGG
567 PflMI CCAn_nnn'nTGG
574 MnlI CCTCnnnnnn_n'
593 BtsI GCAGTG_nn'
598 TspDTI ATGAAnnnnnnnnn_nn'
600 TspRI _nnCAsTGnn'
605 BsmI GAATG_Cn'
629 BslI CCnn_nnn'nnGG
646 BceAI ACGGCnnnnnnnnnnnn'nn_
647 SfcI C'TryA_G
650 MnlI CCTCnnnnnn_n'
661 BsaAI yAC'GTr
663 BslI CCnn_nnn'nnGG
664 BslI CCnn_nnn'nnGG
674 MlyI GAGTCnnnnn'
674 PleI GAGTCnnnn'n_
680 BseRI GAGGAGnnnnnnnn_nn'
701 MnlI CCTCnnnnnn_n'
710 MnlI CCTCnnnnnn_n'
713 BciVI GTATCCnnnnn_n'
738 TspGWI ACGGAnnnnnnnnn_nn'
743 PleI GAGTCnnnn'n_
744 MlyI GAGTCnnnnn'
751 PspOMI G'GGCC_C
753 NlaIV GGn'nCC
755 ApaI G_GGCC'C
755 BanII G_rGCy'C
755 Bme1580I G_kGCm'C
755 Bsp1286I G_dGCh'C
755 BsgI GTGCAGnnnnnnnnnnnnnn_nn'
762 TspRI _nnCAsTGnn'
776 MnlI CCTCnnnnnn_n'
786 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
789 BsaJI C'CnnG_G
790 BslI CCnn_nnn'nnGG
794 MnlI CCTCnnnnnn_n'
803 BanI G'GyrC_C
803 KasI G'GCGC_C
804 BsaHI Gr'CG_yC
804 NarI GG'CG_CC
805 NlaIV GGn'nCC
805 SfoI GGC'GCC
807 BbeI G_GCGC'C
807 HaeII r_GCGC'y
825 MwoI GCnn_nnn'nnGC
826 HpyF10VI GCn_nnnnn'nGC
833 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
833 Hin4I GAbnnnnnrTCnnnnnnnn_nnnnn'
853 FokI GGATGnnnnnnnnn'nnnn_
855 TspDTI ATGAAnnnnnnnnn_nn'
859 TspGWI ACGGAnnnnnnnnn_nn'
865 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
865 Hin4I GAbnnnnnrTCnnnnnnnn_nnnnn'
866 BstF5I GGATG_nn'
882 BglI GCCn_nnn'nGGC
882 MwoI GCnn_nnn'nnGC
883 HpyF10VI GCn_nnnnn'nGC
885 StuI AGG'CCT
920 BclI T'GATC_A
923 TspRI _nnCAsTGnn'
934 AlwI GGATCnnnn'n_
941 BspCNI CTCAGnnnnnnn_nn'
942 BseMII CTCAGnnnnnnnn_nn'
942 BsrDI GCAATG_nn'
955 BsaJI C'CnnG_G
955 BtgI C'CryG_G
955 NcoI C'CATG_G
955 StyI C'CwwG_G
979 MnlI CCTCnnnnnn_n'
1007 BsiWI C'GTAC_G
1017 TspDTI ATGAAnnnnnnnnn_nn'
1020 ApoI r'AATT_y
1020 EcoRI G'AATT_C
1031 MnlI CCTCnnnnnn_n'
1035 BsaXI GGAGnnnnnGTnnnnnnnnn_nnn'
1035 BssSI C'ACGA_G
1041 StuI AGG'CCT
1059 MnlI CCTCnnnnnn_n'
1065 BsaXI ACnnnnnCTCCnnnnnnn_nnn'
1083 Cac8I GCn'nGC
1083 EaeI y'GGCC_r
1085 MscI TGG'CCA
1133 MnlI CCTCnnnnnn_n'
1141 HphI GGTGAnnnnnnn_n'
1145 PsiI TTA'TAA
1166 FokI GGATGnnnnnnnnn'nnnn_
1174 Hpy188III TC'nn_GA
1179 BstF5I GGATG_nn'
1216 TspDTI ATGAAnnnnnnnnn_nn'
1222 MnlI CCTCnnnnnn_n'
1239 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
1241 MnlI CCTCnnnnnn_n'
1270 MnlI CCTCnnnnnn_n'
1271 Hin4I GAbnnnnnrTCnnnnnnnn_nnnnn'
1274 MslI CAynn'nnrTG
1290 PpiI GAGnnnnnGTTCnnnnnnn_nnnnn'
1311 AarI CACCTGCnnnn'nnnn_
1311 BspMI ACCTGCnnnn'nnnn_
1322 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
1332 SspI AAT'ATT
1346 MnlI CCTCnnnnnn_n'
1355 MnlI CCTCnnnnnn_n'
1364 BsaWI w'CCGG_w
1364 BspEI T'CCGG_A
1365 Hpy188III TC'nn_GA

Enzymes that do not cut:

_________________________________________________________
AatII, AccI, Acc65I, AclI, AflII, AflIII, AgeI, AhdI, AleI, AloI, AloI, AlwNI
ApaLI, AscI, AsiSI, AvaI, AvrII, BaeI, BaeI, BamHI, BbsI, BbvCI, BcgI, BcgI, BfrBI
BglII, BlpI, BmgBI, BmrI, BmtI, BplI, BpmI, BpuEI, BsaI, BsaBI, BseYI, BsiEI
BsiHKAI, BsmBI, BsmFI, BspHI, BsrBI, BsrFI, BsrGI, BssHII, BstAPI, BstBI, BstEII
BstXI, BstYI, BstZ17I, Bsu36I, ClaI, DraI, DraIII, DrdI, EagI, EarI, EciI, Eco57I
EcoICRI, Eco57MI, EcoNI, EcoO109I, EcoRV, FalI, FauI, FseI, FspI, FspAI, HgaI
HincII, HindIII, HpaI, Hpy8I, KpnI, MfeI, MluI, MspA1I, NaeI, NdeI, NgoMIV, NheI
NotI, NruI, NsiI, NspI, PacI, PciI, PmeI, PmlI, PpuMI, PshAI, PsrI, PsrI, PstI
PvuI, PvuII, RsrII, SacI, SacII, SalI, SanDI, SapI, SbfI, SexAI, SfiI, SgrAI, SmaI
SmlI, SnaBI, SpeI, SphI, SrfI, SwaI, TaqII, Tth111I, XbaI, XhoI, XmaI, XmnI, ZraI
Align By ClustalW

Align By ClustalW (Codons)

Align By MUSCLE

Align By MUSCLE (Codons)

Phylogenetic tree

Phylogeny with test maximum likelilhood

MK133021.1 A.anser
100%

100% KT427476.1 A.cygnoides

100%
NC 016922.1 A.fabalis KINANTI

100%
KM455570.1 A.indicus

NC 039888.1 A.albifrons
100%

KP981363.1 C.cygnus
99%

KF800698.1 C.columbianus

99%
KR867679.1 C.olor

99%
FJ379295.1 C.atratus

KJ710708.1 A.ferina
100%

AF090337.1 A.americana

100% KT345702.1 A.clypeata

KC759527.1 A.falcata
100%

KF562761.1 A.chathamica
100%

100% KJ778676.1 A.platyrhynchos

100%
KF751616.1 A.poecilorhyncha
Phylogeny with test neighbor-joining

MK133021.1 A.anser
100%

100% KT427476.1 A.cygnoides

100%
NC 016922.1 A.fabalis KINANTI

100%
KM455570.1 A.indicus

NC 039888.1 A.albifrons
100%

KP981363.1 C.cygnus
99%

KF800698.1 C.columbianus

99%
KR867679.1 C.olor

99%
FJ379295.1 C.atratus

KJ710708.1 A.ferina
100%

AF090337.1 A.americana

100% KT345702.1 A.clypeata

KC759527.1 A.falcata
100%

KF562761.1 A.chathamica
100%

100% KJ778676.1 A.platyrhynchos

100%
KF751616.1 A.poecilorhyncha
Phylogeny with test minimum-evolution

MK133021.1 A.anser
100%

100% KT427476.1 A.cygnoides

100%
NC 016922.1 A.fabalis KINANTI

100%
KM455570.1 A.indicus

NC 039888.1 A.albifrons
100%

KP981363.1 C.cygnus
99%

KF800698.1 C.columbianus

99%
KR867679.1 C.olor

99%
FJ379295.1 C.atratus

KJ710708.1 A.ferina
100%

AF090337.1 A.americana

100% KT345702.1 A.clypeata

KC759527.1 A.falcata
100%

KF562761.1 A.chathamica
100%

100% KJ778676.1 A.platyrhynchos

100%
KF751616.1 A.poecilorhyncha