BioEdit version 7.0.5.

3 (10/28/05) Restriction Mapping Utility

(c)1998, Tom Hall

Sri Utami Rudy Restriction Map

9/29/2022 6:31:35 AM
1530 base pairs
Translations: 1

Restriction Enzyme Map:

M T Q K S H Y I S * L I M V P A V N P P T T S N H V
A
1
ATGACACAAAAATCCCATTACATATCCTAACTAATTATGGTACCAGCAGTAAATCCTCCTACCACATCCAACCACGTGGC 80
1
TACTGTGTTTTTAGGGTAATGTATAGGATTGATTAATACCATGGTCGTCATTTAGGAGGATGGTGTAGGTTGGTGCACCG 80
Acc65I FokI BstF5I
BstXI
BanI MnlI
BsaAI
NlaIV
PmlI
KpnI

V I G A G A A G L V A A R E L R R E G H S V V V F E
81
AGTAATCGGTGCTGGAGCAGCCGGACTCGTGGCTGCTCGAGAGCTCCGCCGTGAGGGCCACTCTGTCGTTGTTTTCGAAC 160
81
TCATTAGCCACGACCTCGTCGGCCTGAGCACCGACGAGCTCTCGAGGCGGCACTCCCGGTGAGACAGCAACAAAAGCTTG 160
MmeI HpyF10VI BbvI EciI EcoICRI BslI
BstBI
MwoI BssSI BpmI BplI BglI
BplI
MlyI Eco57MI BanII MwoI
PleI BceAI BsiHKAI HpyF10VI
BbvI AvaI Bsp1286I
SmlI SacI
XhoI MnlI
Hpy188III

R G N H I G G V W A Y T P N V E P D P L S I D P T R
P
161
GTGGGAACCACATCGGAGGCGTGTGGGCTTACACGCCAAACGTTGAACCCGACCCCCTTAGCATCGACCCGACCCGACCC 240
161
CACCCTTGGTGTAGCCTCCGCACACCCGAATGTGCGGTTTGCAACTTGGGCTGGGGGAATCGTAGCTGGGCTGGGCTGGG 240
NlaIV AclI Bpu10I Hin4I
MnlI Hin4I
SfaNI

V I H S S L Y S S L R T I I P Q E C M G F T D F P F
S
241
GTAATCCATTCGAGCCTCTACAGTTCTCTCCGAACCATCATCCCACAAGAATGCATGGGTTTCACTGATTTCCCGTTTTC 320
241
CATTAGGTAAGCTCGGAGATGTCAAGAGAGGCTTGGTAGTAGGGTGTTCTTACGTACCCAAAGTGACTAAAGGGCAAAAG 320
 SfcI MnlI BstF5I MslI BsmI
TspRI
Hin4I BfrBI
Hin4I NsiI
FokI

T R L E N G S R D P R R H P G H S E V L A Y L R D F
321
GACCAGACTCGAAAACGGGTCTAGAGATCCGAGAAGACATCCAGGTCATAGTGAGGTTCTTGCTTACCTGAGAGACTTTG 400
321
CTGGTCTGAGCTTTTGCCCAGATCTCTAGGCTCTTCTGTAGGTCCAGTATCACTCCAAGAACGAATGGACTCTCTGAAAC 400
MlyI XbaI BstF5I MboII Hin4I BsmAI
MnlI
PleI AlwI BbsI MnlI BseMII
Hpy188III DrdI BspCNI
Hin4I
FokI
BstYI

V R E F K I E E M I R F E T E V V R V E Q A G E N P
K
401
TGAGGGAGTTCAAGATTGAGGAGATGATTCGTTTCGAGACGGAGGTTGTGAGGGTTGAGCAGGCGGGGGAAAATCCCAAA 480
401
ACTCCCTCAAGTTCTAACTCCTCTACTAAGCAAAGCTCTGCCTCCAACACTCCCAACTCGTCCGCCCCCTTTTAGGGTTT 480
Hpy188III BsmAI MnlI TspGWI
MnlI BsmBI FauI
BseRI Cac8I
Hpy188III
MnlI

K W R V K S R N F G D I S D E I Y D A V V V C N G H
Y
481
AAGTGGAGAGTCAAGTCTAGAAATTTCGGTGATATCTCCGACGAGATTTATGACGCCGTAGTTGTTTGTAACGGTCACTA 560
481
TTCACCTCTCAGTTCAGATCTTTAAAGCCACTATAGAGGCTGCTCTAAATACTGCGGCATCAACAAACATTGCCAGTGAT 560
BslI PleI EcoRV HphI BsaHI HgaI
PflMI XbaI BceAI MmeI
MlyI
Hpy188III
ApoI

T E P R H A L I P G N K I N H S F S I G L G I D T W
561
TACAGAGCCTCGTCATGCTCTAATACCTGGTAATAAAATTAACCACTCATTTTCCATCGGTCTTGGCATAGATACATGGC 640
561
ATGTCTCGGAGCAGTACGAGATTATGGACCATTATTTTAATTGGTGAGTAAAAGGTAGCCAGAACCGTATCTATGTACCG 640
MnlI SexAI TaqII
EaeI

MscI
 P G K Q I H S H N Y R V P E Q V K D Q V V V V I G S
S
641
CAGGAAAGCAAATCCACAGCCACAATTATCGAGTTCCAGAACAAGTTAAAGATCAGGTGGTGGTGGTGATTGGAAGCTCA 720
641
GTCCTTTCGTTTAGGTGTCGGTGTTAATAGCTCAAGGTCTTGTTCAATTTCTAGTCCACCACCACCACTAACCTTCGAGT 720
MwoI Hpy188III
HphI
HpyF10VI

V S G V D I S R D I A N V T K E V H I S S R S T K P
E
721
GTGAGCGGAGTCGATATAAGCAGAGACATAGCAAATGTTACAAAAGAAGTCCATATATCTTCAAGATCAACTAAACCGGA 800
721
CACTCGCCTCAGCTATATTCGTCTCTGTATCGTTTACAATGTTTTCTTCAGGTATATAGAAGTTCTAGTTGATTTGGCCT 800
MwoI BspCNI MboII Hpy188III
BsaWI
HpyF10VI PleI
TspRI MlyI
BsrBI BsmAI
BseMII

T Y E K L P G Y D N L W L H S N I E T V R E D G S V
801
AACATACGAAAAACTTCCCGGTTACGACAACTTATGGCTTCACTCTAATATTGAAACTGTCCGAGAAGATGGTTCGGTGG 880
801
TTGTATGCTTTTTGAAGGGCCAATGCTGTTGAATACCGAAGTGAGATTATAACTTTGACAGGCTCTTCTACCAAGCCACC 880
XmnI SspI
MboII

V F K N G K T V Y A D T I M H C T G Y K Y Y F P F L
D
881
TGTTTAAGAACGGAAAGACGGTTTACGCGGATACCATTATGCATTGCACCGGGTACAAATATTACTTCCCATTTCTCGAC 960
881
ACAAATTCTTGCCTTTCTGCCAAATGCGCCTATGGTAATACGTAACGTGGCCCATGTTTATAATGAAGGGTAAAGAGCTG 960
Hpy8I BfrBI SspI
Hpy188III
BciVI BsrDI
TspGWI NsiI

T K G E V T V E D N R V G P L Y K H V F P P A L S P
G
961
ACGAAAGGCGAAGTGACCGTTGAGGATAACCGTGTTGGACCATTATATAAGCATGTTTTCCCACCAGCTCTTTCTCCCGG
1040
961
TGCTTTCCGCTTCACTGGCAACTCCTATTGGCACAACCTGGTAATATATTCGTACAAAAGGGTGGTCGAGAAAGAGGGCC
1040
MmeI NspI
TspDTI
 MnlI
TspDTI

L S F I G L P W Q N M K L Q T L D V N E L I G Q C F
1041
ACTTTCATTCATTGGATTACCATGGCAGAATATGAAGTTACAAACTCTTGATGTCAATGAACTTATTGGTCAATGTTTCG
1120
1041
TGAAAGTAAGTAACCTAATGGTACCGTCTTATACTTCAATGTTTGAGAACTACAGTTACTTGAATAACCAGTTACAAAGC
1120
BsaJI TspDTI
TspDTI
BtgI Hpy188III
NcoI
StyI

G Y L L V I P F P M F E L Q S K W V A A V L A G R V
S
1121
GATATTTATTGGTCATTCCTTTTCCAATGTTTGAACTCCAAAGCAAATGGGTAGCCGCGGTTTTGGCAGGTAGGGTTTCA
1200
1121
CTATAAATAACCAGTAAGGAAAAGGTTACAAACTTGAGGTTTCGTTTACCCATCGGCGCCAAAACCGTCCATCCCAAAGT
1200
BsaJI MwoI
BtgI HpyF10VI
MspA1I
BspMI
SacII

L P S Q E E M E D T K M F Y L K L E A S C I P K R Y
T
1201
CTCCCCTCACAAGAAGAGATGGAAGATACTAAGATGTTTTATTTGAAGCTCGAAGCCTCATGTATTCCCAAAAGATACAC
1280
1201
GAGGGGAGTGTTCTTCTCTACCTTCTATGATTCTACAAAATAAACTTCGAGCTTCGGAGTACATAAGGGTTTTCTATGTG
1280
EarI MnlI MboII MboII MnlI

H L M A E L D S Q F V Y N N W L A D Q C D Y P R I E
1281
ACATCTTATGGCAGAATTAGATTCTCAGTTTGTATACAATAACTGGCTTGCTGATCAATGTGACTATCCTCGGATCGAGA
1360
1281
TGTAGAATACCGTCTTAATCTAAGAGTCAAACATATGTTATTGACCGAACGACTAGTTACACTGATAGGAGCCTAGCTCT
1360
AccI BsrI BsaJI
Hpy188III
BstZ17I Cac8I
MnlI
Hpy8I BclI
BspCNI
BseMII
 K W R E Q M F Y K V F K R I Q S Q A S T Y K D D W D
D
1361
AATGGAGAGAGCAAATGTTTTACAAGGTGTTCAAGAGAATACAATCCCAAGCCAGTACATATAAGGACGATTGGGACGAT
1440
1361
TTACCTCTCTCGTTTACAAAATGTTCCACAAGTTCTCTTATGTTAGGGTTCGGTCATGTATATTCCTGCTAACCCTGCTA
1440
AlwI Hpy188III BsrI
HphI
TatI

D H L I A E A Y E D F V K F P S N Y P S S L I E R E
Y
1441
GATCACCTCATCGCAGAAGCATATGAAGATTTCGTCAAATTCCCATCTAACTATCCCAGTTCTTTAATCGAAAGAGAATA
1520
1441
CTAGTGGAGTAGCGTCTTCGTATACTTCTAAAGCAGTTTAAGGGTAGATTGATAGGGTCAAGAAATTAGCTTTCTCTTAT
1520
BclI BsmFI MnlI MboII BmrI BsrI
NdeI ApoI
TspDTI

T S *
1521 TACAAGCTGA 1530
1521 ATGTTCGACT 1530

Restriction table:

Enzyme Recognition frequency Positions

__________________________________________________________________________
AccI GT'mk_AC 1 1314
Acc65I G'GTAC_C 1 40
AclI AA'CG_TT 1 201
AlwI GGATCnnnn'n_ 2 341, 1361
ApoI r'AATT_y 2 502, 1478
AvaI C'yCGr_G 1 117
BanI G'GyrC_C 1 40
BanII G_rGCy'C 1 126
BbsI GAAGACnn'nnnn_ 1 361
BbvI GCAGCnnnnnnnn'nnnn_ 2 110, 100
BceAI ACGGCnnnnnnnnnnnn'nn_ 2 114, 521
BciVI GTATCCnnnnn_n' 1 904
BclI T'GATC_A 2 1333, 1441
BfrBI ATG'CAT 2 294, 922
BglI GCCn_nnn'nGGC 1 135
BmrI ACTGGGnnnn_n' 1 1491
BplI GAGnnnnnCTCnnnnnnnn_nnnnn' 2 125, 157
BpmI CTGGAGnnnnnnnnnnnnnn_nn' 1 114
Bpu10I CC'TnA_GC 1 218
BsaAI yAC'GTr 1 76
BsaHI Gr'CG_yC 1 534
BsaJI C'CnnG_G 3 1061, 1176, 1349
BsaWI w'CCGG_w 1 796
BseMII CTCAGnnnnnnnn_nn' 3 380, 732, 1319
BseRI GAGGAGnnnnnnnn_nn' 1 434
BsiHKAI G_wGCw'C 1 126
BslI CCnn_nnn'nnGG 2 133, 483
BsmI GAATG_Cn' 1 296
BsmAI GTCTCn'nnnn_ 3 387, 431, 738
BsmBI CGTCTCn'nnnn_ 1 431
BsmFI GGGACnnnnnnnnnn'nnnn_ 1 1448
Bsp1286I G_dGCh'C 1 126
BspCNI CTCAGnnnnnnn_nn' 3 381, 731, 1318
BspMI ACCTGCnnnn'nnnn_ 1 1178
BsrI ACTG_Gn' 3 1328, 1413, 1497
BsrBI CCG'CTC 1 726
BsrDI GCAATG_nn' 1 922
BssSI C'ACGA_G 1 107
BstBI TT'CG_AA 1 156
BstF5I GGATG_nn' 3 65, 279, 358
BstXI CCAn_nnnn'nTGG 1 76
BstYI r'GATC_y 1 346
BstZ17I GTA'TAC 1 1315
BtgI C'CryG_G 2 1061, 1176
Cac8I GCn'nGC 2 462, 1329
DrdI GACnn_nn'nnGTC 1 363
EaeI y'GGCC_r 1 638
EarI CTCTTCn'nnn_ 1 1209
EciI GGCGGAnnnnnnnnn_nn' 1 116
EcoICRI GAG'CTC 1 124
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' 1 114
EcoRV GAT'ATC 1 514
FauI CCCGCnnnn'nn_ 1 457
FokI GGATGnnnnnnnnn'nnnn_ 3 52, 266, 345
HgaI GACGCnnnnn'nnnnn_ 1 542
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' 3 228, 260, 343
Hin4I GAbnnnnnrTCnnnnnnnn_nnnnn' 3 228, 260, 375
HphI GGTGAnnnnnnn_n' 3 521, 718, 1436
Hpy8I GTn'nAC 2 904, 1315
Hpy188III TC'nn_GA 11 119, 342, 412, 436, 498, 677
783, 956, 1088, 1357, 1393
HpyF10VI GCn_nnnnn'nGC 5 99, 136, 647, 724, 1185
KpnI G_GTAC'C 1 44
MboII GAAGAnnnnnnn_n' 6 366, 771, 878, 1226, 1235, 1478
MlyI GAGTCnnnnn' 4 99, 321, 498, 738
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' 3 93, 543, 976
MnlI CCTCnnnnnn_n' 15 66, 127, 170, 266, 367, 396, 412
436, 444, 579, 976, 1216, 1267
1359, 1457
MscI TGG'CCA 1 640
MslI CAynn'nnrTG 1 289
MspA1I CmG'CkG 1 1178
MwoI GCnn_nnn'nnGC 5 98, 135, 646, 723, 1184
NcoI C'CATG_G 1 1061
NdeI CA'TA_TG 1 1462
NlaIV GGn'nCC 2 42, 167
NsiI A_TGCA'T 2 296, 924
NspI r_CATG'y 1 1016
PflMI CCAn_nnn'nTGG 1 483
PleI GAGTCnnnn'n_ 4 99, 321, 497, 737
PmlI CAC'GTG 1 76
SacI G_AGCT'C 1 126
SacII CC_GC'GG 1 1179
SexAI A'CCwGG_T 1 586
SfaNI GCATCnnnnn'nnnn_ 1 231
SfcI C'TryA_G 1 259
SmlI C'TyrA_G 1 117
SspI AAT'ATT 2 850, 941
StyI C'CwwG_G 1 1061
TaqII GACCGAnnnnnnnnn_nn' 1 608
TatI w'GTAC_w 1 1415
TspDTI ATGAAnnnnnnnnn_nn' 5 1035, 1039, 1088, 1113, 1479
TspGWI ACGGAnnnnnnnnn_nn' 2 455, 906
TspRI _nnCAsTGnn' 2 310, 726
XbaI T'CTAG_A 2 341, 497
XhoI C'TCGA_G 1 117
XmnI GAAnn'nnTTC 1 813

Enzymes that cut five or fewer times

Enzyme Recognition frequency Positions

__________________________________________________________________________
AccI GT'mk_AC 1 1314
Acc65I G'GTAC_C 1 40
AclI AA'CG_TT 1 201
AlwI GGATCnnnn'n_ 2 341, 1361
ApoI r'AATT_y 2 502, 1478
AvaI C'yCGr_G 1 117
BanI G'GyrC_C 1 40
BanII G_rGCy'C 1 126
BbsI GAAGACnn'nnnn_ 1 361
BbvI GCAGCnnnnnnnn'nnnn_ 2 110, 100
BceAI ACGGCnnnnnnnnnnnn'nn_ 2 114, 521
BciVI GTATCCnnnnn_n' 1 904
BclI T'GATC_A 2 1333, 1441
BfrBI ATG'CAT 2 294, 922
BglI GCCn_nnn'nGGC 1 135
BmrI ACTGGGnnnn_n' 1 1491
BplI GAGnnnnnCTCnnnnnnnn_nnnnn' 2 125, 157
BpmI CTGGAGnnnnnnnnnnnnnn_nn' 1 114
Bpu10I CC'TnA_GC 1 218
BsaAI yAC'GTr 1 76
BsaHI Gr'CG_yC 1 534
BsaJI C'CnnG_G 3 1061, 1176, 1349
BsaWI w'CCGG_w 1 796
BseMII CTCAGnnnnnnnn_nn' 3 380, 732, 1319
BseRI GAGGAGnnnnnnnn_nn' 1 434
BsiHKAI G_wGCw'C 1 126
BslI CCnn_nnn'nnGG 2 133, 483
BsmI GAATG_Cn' 1 296
BsmAI GTCTCn'nnnn_ 3 387, 431, 738
BsmBI CGTCTCn'nnnn_ 1 431
BsmFI GGGACnnnnnnnnnn'nnnn_ 1 1448
Bsp1286I G_dGCh'C 1 126
BspCNI CTCAGnnnnnnn_nn' 3 381, 731, 1318
BspMI ACCTGCnnnn'nnnn_ 1 1178
BsrI ACTG_Gn' 3 1328, 1413, 1497
BsrBI CCG'CTC 1 726
BsrDI GCAATG_nn' 1 922
BssSI C'ACGA_G 1 107
BstBI TT'CG_AA 1 156
BstF5I GGATG_nn' 3 65, 279, 358
BstXI CCAn_nnnn'nTGG 1 76
BstYI r'GATC_y 1 346
BstZ17I GTA'TAC 1 1315
BtgI C'CryG_G 2 1061, 1176
Cac8I GCn'nGC 2 462, 1329
DrdI GACnn_nn'nnGTC 1 363
EaeI y'GGCC_r 1 638
EarI CTCTTCn'nnn_ 1 1209
EciI GGCGGAnnnnnnnnn_nn' 1 116
EcoICRI GAG'CTC 1 124
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' 1 114
EcoRV GAT'ATC 1 514
FauI CCCGCnnnn'nn_ 1 457
FokI GGATGnnnnnnnnn'nnnn_ 3 52, 266, 345
HgaI GACGCnnnnn'nnnnn_ 1 542
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' 3 228, 260, 343
Hin4I GAbnnnnnrTCnnnnnnnn_nnnnn' 3 228, 260, 375
HphI GGTGAnnnnnnn_n' 3 521, 718, 1436
Hpy8I GTn'nAC 2 904, 1315
HpyF10VI GCn_nnnnn'nGC 5 99, 136, 647, 724, 1185
KpnI G_GTAC'C 1 44
MlyI GAGTCnnnnn' 4 99, 321, 498, 738
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' 3 93, 543, 976
MscI TGG'CCA 1 640
MslI CAynn'nnrTG 1 289
MspA1I CmG'CkG 1 1178
MwoI GCnn_nnn'nnGC 5 98, 135, 646, 723, 1184
NcoI C'CATG_G 1 1061
NdeI CA'TA_TG 1 1462
NlaIV GGn'nCC 2 42, 167
NsiI A_TGCA'T 2 296, 924
NspI r_CATG'y 1 1016
PflMI CCAn_nnn'nTGG 1 483
PleI GAGTCnnnn'n_ 4 99, 321, 497, 737
PmlI CAC'GTG 1 76
SacI G_AGCT'C 1 126
SacII CC_GC'GG 1 1179
SexAI A'CCwGG_T 1 586
SfaNI GCATCnnnnn'nnnn_ 1 231
SfcI C'TryA_G 1 259
SmlI C'TyrA_G 1 117
SspI AAT'ATT 2 850, 941
StyI C'CwwG_G 1 1061
TaqII GACCGAnnnnnnnnn_nn' 1 608
TatI w'GTAC_w 1 1415
TspDTI ATGAAnnnnnnnnn_nn' 5 1035, 1039, 1088, 1113, 1479
TspGWI ACGGAnnnnnnnnn_nn' 2 455, 906
TspRI _nnCAsTGnn' 2 310, 726
XbaI T'CTAG_A 2 341, 497
XhoI C'TCGA_G 1 117
XmnI GAAnn'nnTTC 1 813

Position Enzyme(s)
__________________________________________________________________________
40 Acc65I G'GTAC_C
40 BanI G'GyrC_C
42 NlaIV GGn'nCC
44 KpnI G_GTAC'C
52 FokI GGATGnnnnnnnnn'nnnn_
65 BstF5I GGATG_nn'
66 MnlI CCTCnnnnnn_n'
76 BstXI CCAn_nnnn'nTGG
76 BsaAI yAC'GTr
76 PmlI CAC'GTG
93 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
98 MwoI GCnn_nnn'nnGC
99 HpyF10VI GCn_nnnnn'nGC
99 MlyI GAGTCnnnnn'
99 PleI GAGTCnnnn'n_
100 BbvI GCAGCnnnnnnnn'nnnn_
107 BssSI C'ACGA_G
110 BbvI GCAGCnnnnnnnn'nnnn_
114 BpmI CTGGAGnnnnnnnnnnnnnn_nn'
114 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
114 BceAI ACGGCnnnnnnnnnnnn'nn_
116 EciI GGCGGAnnnnnnnnn_nn'
117 AvaI C'yCGr_G
117 SmlI C'TyrA_G
117 XhoI C'TCGA_G
119 Hpy188III TC'nn_GA
124 EcoICRI GAG'CTC
125 BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
126 BanII G_rGCy'C
126 BsiHKAI G_wGCw'C
126 Bsp1286I G_dGCh'C
126 SacI G_AGCT'C
127 MnlI CCTCnnnnnn_n'
133 BslI CCnn_nnn'nnGG
135 BglI GCCn_nnn'nGGC
135 MwoI GCnn_nnn'nnGC
136 HpyF10VI GCn_nnnnn'nGC
156 BstBI TT'CG_AA
157 BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
167 NlaIV GGn'nCC
170 MnlI CCTCnnnnnn_n'
201 AclI AA'CG_TT
218 Bpu10I CC'TnA_GC
228 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
228 Hin4I GAbnnnnnrTCnnnnnnnn_nnnnn'
231 SfaNI GCATCnnnnn'nnnn_
259 SfcI C'TryA_G
260 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
260 Hin4I GAbnnnnnrTCnnnnnnnn_nnnnn'
266 MnlI CCTCnnnnnn_n'
266 FokI GGATGnnnnnnnnn'nnnn_
279 BstF5I GGATG_nn'
289 MslI CAynn'nnrTG
294 BfrBI ATG'CAT
296 BsmI GAATG_Cn'
296 NsiI A_TGCA'T
310 TspRI _nnCAsTGnn'
321 MlyI GAGTCnnnnn'
321 PleI GAGTCnnnn'n_
341 XbaI T'CTAG_A
341 AlwI GGATCnnnn'n_
342 Hpy188III TC'nn_GA
343 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
345 FokI GGATGnnnnnnnnn'nnnn_
346 BstYI r'GATC_y
358 BstF5I GGATG_nn'
361 BbsI GAAGACnn'nnnn_
363 DrdI GACnn_nn'nnGTC
366 MboII GAAGAnnnnnnn_n'
367 MnlI CCTCnnnnnn_n'
375 Hin4I GAbnnnnnrTCnnnnnnnn_nnnnn'
380 BseMII CTCAGnnnnnnnn_nn'
381 BspCNI CTCAGnnnnnnn_nn'
387 BsmAI GTCTCn'nnnn_
396 MnlI CCTCnnnnnn_n'
412 Hpy188III TC'nn_GA
412 MnlI CCTCnnnnnn_n'
431 BsmAI GTCTCn'nnnn_
431 BsmBI CGTCTCn'nnnn_
434 BseRI GAGGAGnnnnnnnn_nn'
436 Hpy188III TC'nn_GA
436 MnlI CCTCnnnnnn_n'
444 MnlI CCTCnnnnnn_n'
455 TspGWI ACGGAnnnnnnnnn_nn'
457 FauI CCCGCnnnn'nn_
462 Cac8I GCn'nGC
483 BslI CCnn_nnn'nnGG
483 PflMI CCAn_nnn'nTGG
497 PleI GAGTCnnnn'n_
497 XbaI T'CTAG_A
498 MlyI GAGTCnnnnn'
498 Hpy188III TC'nn_GA
502 ApoI r'AATT_y
514 EcoRV GAT'ATC
521 HphI GGTGAnnnnnnn_n'
521 BceAI ACGGCnnnnnnnnnnnn'nn_
534 BsaHI Gr'CG_yC
542 HgaI GACGCnnnnn'nnnnn_
543 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
579 MnlI CCTCnnnnnn_n'
586 SexAI A'CCwGG_T
608 TaqII GACCGAnnnnnnnnn_nn'
638 EaeI y'GGCC_r
640 MscI TGG'CCA
646 MwoI GCnn_nnn'nnGC
647 HpyF10VI GCn_nnnnn'nGC
677 Hpy188III TC'nn_GA
718 HphI GGTGAnnnnnnn_n'
723 MwoI GCnn_nnn'nnGC
724 HpyF10VI GCn_nnnnn'nGC
726 TspRI _nnCAsTGnn'
726 BsrBI CCG'CTC
731 BspCNI CTCAGnnnnnnn_nn'
732 BseMII CTCAGnnnnnnnn_nn'
737 PleI GAGTCnnnn'n_
738 MlyI GAGTCnnnnn'
738 BsmAI GTCTCn'nnnn_
771 MboII GAAGAnnnnnnn_n'
783 Hpy188III TC'nn_GA
796 BsaWI w'CCGG_w
813 XmnI GAAnn'nnTTC
850 SspI AAT'ATT
878 MboII GAAGAnnnnnnn_n'
904 Hpy8I GTn'nAC
904 BciVI GTATCCnnnnn_n'
906 TspGWI ACGGAnnnnnnnnn_nn'
922 BfrBI ATG'CAT
922 BsrDI GCAATG_nn'
924 NsiI A_TGCA'T
941 SspI AAT'ATT
956 Hpy188III TC'nn_GA
976 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
976 MnlI CCTCnnnnnn_n'
1016 NspI r_CATG'y
1035 TspDTI ATGAAnnnnnnnnn_nn'
1039 TspDTI ATGAAnnnnnnnnn_nn'
1061 BsaJI C'CnnG_G
1061 BtgI C'CryG_G
1061 NcoI C'CATG_G
1061 StyI C'CwwG_G
1088 TspDTI ATGAAnnnnnnnnn_nn'
1088 Hpy188III TC'nn_GA
1113 TspDTI ATGAAnnnnnnnnn_nn'
1176 BsaJI C'CnnG_G
1176 BtgI C'CryG_G
1178 MspA1I CmG'CkG
1178 BspMI ACCTGCnnnn'nnnn_
1179 SacII CC_GC'GG
1184 MwoI GCnn_nnn'nnGC
1185 HpyF10VI GCn_nnnnn'nGC
1209 EarI CTCTTCn'nnn_
1216 MnlI CCTCnnnnnn_n'
1226 MboII GAAGAnnnnnnn_n'
1235 MboII GAAGAnnnnnnn_n'
1267 MnlI CCTCnnnnnn_n'
1314 AccI GT'mk_AC
1315 BstZ17I GTA'TAC
1315 Hpy8I GTn'nAC
1318 BspCNI CTCAGnnnnnnn_nn'
1319 BseMII CTCAGnnnnnnnn_nn'
1328 BsrI ACTG_Gn'
1329 Cac8I GCn'nGC
1333 BclI T'GATC_A
1349 BsaJI C'CnnG_G
1357 Hpy188III TC'nn_GA
1359 MnlI CCTCnnnnnn_n'
1361 AlwI GGATCnnnn'n_
1393 Hpy188III TC'nn_GA
1413 BsrI ACTG_Gn'
1415 TatI w'GTAC_w
1436 HphI GGTGAnnnnnnn_n'
1441 BclI T'GATC_A
1448 BsmFI GGGACnnnnnnnnnn'nnnn_
1457 MnlI CCTCnnnnnn_n'
1462 NdeI CA'TA_TG
1478 MboII GAAGAnnnnnnn_n'
1478 ApoI r'AATT_y
1479 TspDTI ATGAAnnnnnnnnn_nn'
1491 BmrI ACTGGGnnnn_n'
1497 BsrI ACTG_Gn'

Enzymes that do not cut:

_________________________________________________________
AarI, AatII, AfeI, AflII, AflIII, AgeI, AhdI, AleI, AloI, AloI, AlwNI, ApaI, ApaLI
AscI, AseI, AsiSI, AvrII, BaeI, BaeI, BamHI, BbeI, BbvCI, BcgI, BcgI, BglII, BlpI
Bme1580I, BmgBI, BmtI, BpuEI, BsaI, BsaBI, BsaXI, BsaXI, BseYI, BsgI, BsiEI, BsiWI
BspEI, BspHI, BsrFI, BsrGI, BssHII, BstAPI, BstEII, Bsu36I, BtsI, ClaI, DraI
DraIII, EagI, Eco57I, EcoNI, EcoO109I, EcoRI, FalI, FseI, FspI, FspAI, HaeII
HincII, HindIII, HpaI, KasI, MfeI, MluI, NaeI, NarI, NgoMIV, NheI, NotI, NruI
PacI, PciI, PmeI, PpiI, PpiI, PpuMI, PshAI, PsiI, PspOMI, PsrI, PsrI, PstI, PvuI
PvuII, RsrII, SalI, SanDI, SapI, SbfI, ScaI, SfiI, SfoI, SgrAI, SmaI, SnaBI, SpeI
SphI, SrfI, StuI, SwaI, TaqII, Tth111I, XcmI, XmaI, ZraI