Antibacterial effect

The antimicrobial properties of AgNP’s evaluated against S. aureus and E. coli are shown in

figure 3 a) and b) respectively. All the AgNP`s solutions (1, 5, 9, 15 and 20 mM) show inhibitory

effect. Figure 3 (c) shows a comparative graph of the antimicrobial behavior of AgNP`s against the S.

aureus and E. coli. In both cases, t hese results confirmed the antibacterial properties of AgNP´s. Table

1 displays the result of the inhibition assay obtained.

Table 1. Antibacterial activity of silver nanoparticles synthesized by Melissa officinalis extract

AgNP`s concentration
Inhibition Zone (mm)
(mM) Staphylococcus
Escherichia coli
aureu
s
1.0 6.0 5.0
3.0 6.5 6.0
5.0 8.0 7.0
9.0 10.0 6.0
15. 11.0 12.0
In the case of the S. aureus assay, the magnitude of the inhibition zone exhibits a lineal behavior in the range from

6 to11.5 mm. Regarding to the test against E. coli, the response to the inhibition zone were observed from the

sample performed at 3 mM of AgNO3. In the range of the 3 to 9 mM, variations in the inhibition zone were minimal.

It has been reported that the AgNP´s have the capacity to hold fast to the bacterial cell membrane and penetrate into

the cytoplasm[3]. This fact produce structural changes in the cell, and consequently the elimination of this organism.

In

this sense, can be suppose the cell membrane of the E. Coli exhibits a major resistance to the AgNP`s in low

concentration. Moreover, the AgNP`s synthesized with concentration of 15 and 20 mM of AgNO 3, shows the highest

antibacterial activity. In contrast, cell membrane of the S. aureus exhibit an immediate antibacterial activity from

low concentration and with a uniform activity[18–21].

IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS)

DOI: 10.9790/3008-1303026875 www.iosrjournals.org 3 | Page