Recent investigations[8] revealed that this plant contains metabolites and phenolic compound as gallic acid,

benzoic acid and flavonoids. These organic components can play the role of reducing agent by the oxidation of

aldehyde groups in the molecules to carboxylic acids[2].

In this regard, the evidence of the organic compounds associated to the Melissa officinalis is showed by a FT-IR

-1
spectra of AgNP’s obtained (figure 2 c). The band located at 3285 cm is associated to the stretching vibrations of

-1
hydroxyl (O-H) functional group of the phenols. The intensity observed at 2936 cm corresponds to the C-H bonds

-1
of alkanes. Also, the band located at 2360 cm can be correlated with interaction of the partial positive charges of

-1
the AgNP´s and the hydroxyl groups presents in the gallic acid[14]. The peak at 1644 cm may belong to the C=C

bonds of flavonoids. The bonds of CO-CH3 are represented by the intensity situated at 1416
DOI: 10.3109/1040841X.2014.912200 Silver nanoparticles 2

-1 -1
cm , which is associated to the aromatic compounds. Finally, the intense peak at 1035 cm

corresponds to the C-O bond. In this sense, it is possible to carry out reduction of Ag ions by

Melissa officinalis extract, and this result confirms the presence of organic compounds

associated to the synthesis of AgNP´s[5,15,16].

Figure 2 d) shows the Raman spectra of AgNP´s synthesized at 1, 5,9,15 and 20 mM. The

-1
band situated at 158 cm shows clearly the presence of the Ag lattice vibrations models[17].

-1
In this spectra can be appreciated the presence of the nitrate (NO 3 ) ion, the intensities

-1
observed at 698, 717 and 791 cm correspond to vibration of the doubly generated N-O in

plane [1,8]. Complementarily, the presence of the Ag nanoparticles is demonstrated by the

−1
bands situated at 1319 and 1643 cm [1,4].