Give at least two (2) clinical laboratory procedures that utilize the principle of particulate

gravimetry. Describe each procedure comprehensively.

1. Microbiological Water Testing
Water microbiological testing is carried out through particulate gravimetry. An
adequate sample volume is passed through a sterilized0.45-μm membrane filter for the
analysis of coliform bacteria. Then the filter is placed on a sterilized absorbent pad saturated
with a culture medium and incubated at 35 ±0.5 ° C for 22-24 hours. The presence of
individual bacterial colonies that develop during the incubation period indicates coliform
bacteria.
2. Chocolate's crude fat content
For the quantitative analysis of food samples, various standard approaches are
based on the calculation of the mass of the sample after selective solvent extraction. By
extracting with ether for 16 hours in a Soxhlet extractor, the crude fat content can be
determined in chocolate. The ether is allowed to evaporate after extraction is complete, and
the residue is weighed at 100 ° C after drying. This analysis was also conducted indirectly
by measuring a sample with supercritical CO2 before and after extraction.

Reference:

Harvey, D. (2000). Modern Analytical Chemistry (1st ed.). McGraw-Hill Education: USA