1.

Teasing/Dissociation – is a process
whereby a selected tissue specimen is
immersed in a watch glass containing
isotonic salt solution, carefully
dissected or separated and examined
unstained under microscope
2. Squash preparation – process whereby
small pieces of tissue not more than 1mm
in diameter placed in a microscopic slide
and forcibly compressed with another
slide with a cover glass.
3. Smear preparation – process of
examining sections whereby cellular
material are spread lightly over a slide by
means of a wire loop or application. This
is specifically useful in cytological
examinations particularly cancer
diagnosis.
A. Streaking
With a applicator stick or platinum
loop, the material is rapidly and gently
applied in a zigzag line throughout the
slide, attempting to obtain a relatively
uniform distribution of secretion. Too
thin or thick smears have to be avoided,
since they make the tissue unstable for
examination.
B. Spreading
A selection portion of the material is
transferred to a clean slide and gently spread
into a moderately thick film by teasing the
mucous strands apart with an applicator stick.
This method is a little more tedious than
streaking, but has the advantage of maintaining
cellular interrelationship of the material to be
examined. It is especially recommended for
smear preparation of fresh sputum and bronchial
aspirates, and also for thick mucoid secretions.
C. Pull-apart
D. Touch preparation
4. Frozen section – this method normally
utilized when a rapid diagnosis of the
tissue is question is required
1. Fixation
2. Dehydration
3. Clearing
4. Infiltration
5. Embedding
6. Section Cutting
7. Staining
8. Mounting
9. Labelling