Depth of Focus

Which colored thread comes into focus first, second, and third (as observed under 10x)

The order of the threads are as follows: pink comes first, which is in the bottom, then the blue one, and
lastly, yellow which is at the top. Based on our observation, the yellow thread comes into focus first,
then the middle one which is the blue thread, and finally the pink thread.

As observed under 40x and 4x objectives.

When viewed under the HPO (40x magnification), yellow thread was on top among the other two
colored threads. They showcase hairy-like projections. In addition, the surrounded parts were somewhat
blurry to successfully observe. On the other hand, when viewed under 4x, we saw all the three colored
threads and their arrangements were from the yellow thread being on top then blue thread in the
middle and pink thread as the last one.

Depth of Field
- the distance along the optical axis throughout which the object can be located and yet be imaged with
satisfactory clarity. This is used in stereo microscopy.

Koehler Illumination
- a technique for uniformly illuminating a field from non-uniform light such as a coiled filament lamp.

Magnification
- the enlargement of an object through the lens system. This is determined by multiplying the magnifying
power of the objective by the eyepiece.

Parcentered
- When all the elements of the optical system are aligned on a single axis thus reducing aberration.

Parfocal (parfocality)
- a term used describing the property of a microscope where the subject stays in focus when the objective
lenses are changed. Less then 1/2 of a revolution from the fine adjustment is usually acceptable.
Planachromat
- an achromat lens which has been corrected for a flat field.
Resolution
- a measure of the ability of a lens to image closely spaced objects so they are recognized as separate
objets.
Resolving power
- the capacity of any optical system to distinguish and separate details in a specimen.
Total magnification
- the final magnification of the microscope is the result of objective magnification x eyepiece magnification
with consideration of the tube factor.

A microscope is an instrument used to see objects that are too small to be seen by the naked
eye. 

Microscopy is the science of investigating small objects and structures using such an
instrument.