INTRODUCTION

In chemistry, spectrophotometry is the quantitative measurement of the reflection

or transmission properties of a material as a function of wavelength. It is more
specific than the general term electromagnetic spectroscopy in that spectrophotometry
deals with visible light, near-ultraviolet, and near-infrared, but does not cover time-
resolved spectroscopic techniques. A spectrophotometer is a photometer that can
measure intensity as a function of the light source wavelength. Important features of
spectrophotometers are spectral bandwidth and linear range of absorption or
reflectance measurement.

In this experiment, the absorbance value of the known Cu2+ solution was
determined by using the spectrophotometer. Graph of absorbance versus
concentration of the known solution was plotted. This graph was explained about the
relationship of the concentration and absorbance. As the concentration of solution is
increase, the absorbance value will become greater. It is proved that the concentration
is directly proportional to the absorbance.