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RESULTS

Test tube no. Concentration (M) Absorbance


1. 0.0000 0.066
2. 0.0600 0.257
3. 0.1400 0.397
4. 0.2000 0.621
5. 0.0058 0.082
6. 0.0263 0.139

CALCULATION

Concentration
Test tube No.
M1V1 = M2V2
0 × 0.2 = 0.01M2
1.
M2 = 0.000 M
0.004 × 0.2 = 0.01M2
2.
M2 = 0.080 M
0.007 × 0.2 = 0.01M2
3.
M2 = 0.140 M
0.010 × 0.2 = 0.01M2
4.
M2 = 0.200 M
(0.082 – 0.066) ÷ 2.775
5.
= 0.0058 M
(0.139 – 0.066) ÷ 2.775
6.
= 0.0263 M

Y = mx + c

At point of (0.000, 0.066)


0.066 = (0.000 × m) + c

c = 0.066

At point (0.200, 0.621)

0.621 = m (0.200) + 0.066

m = 2.775

Therefore, the equation is Y = 2.775x + c

Graph of Absorbance vs Concentration


0.7

0.6

0.5
Absorbance

0.4

0.3

0.2

0.1

0
0 0.05 0.1 0.15 0.2 0.25
Concentration/ M

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