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  • University of California, Berkeley Quagga Svante Pääbo Mummified Bacterial Cloning Polymerase Chain Reaction Nested PCR

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    Jeamea Eyano
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    The first study of ancient DNA (aDNA) was conducted in 1984 when researchers at the University of California, Berkeley were able to extract and sequence DNA traces from a 150-year-old museum specimen of a quagga. Over the next two years, Svante Pääbo confirmed this phenomenon could also be replicated in naturally and artificially mummified human samples up to several thousand years old. While the processes for sequencing ancient DNA were initially laborious, the development of polymerase chain reaction in the late 1980s allowed the field to progress rapidly, though double primer PCR amplification presented issues that were later addressed using a nested multiple primer strategy.

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    The first study of ancient DNA (aDNA) was conducted in 1984 when researchers at the University of California, Berkeley were able to extract and sequence DNA traces from a 150-year-old museum specimen of a quagga. Over the next two years, Svante Pääbo confirmed this phenomenon could also be replicated in naturally and artificially mummified human samples up to several thousand years old. While the processes for sequencing ancient DNA were initially laborious, the development of polymerase chain reaction in the late 1980s allowed the field to progress rapidly, though double primer PCR amplification presented issues that were later addressed using a nested multiple primer strategy.

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    9 views1 page

    University of California, Berkeley Quagga Svante Pääbo Mummified Bacterial Cloning Polymerase Chain Reaction Nested PCR

    Uploaded by

    Jeamea Eyano
    The first study of ancient DNA (aDNA) was conducted in 1984 when researchers at the University of California, Berkeley were able to extract and sequence DNA traces from a 150-year-old museum specimen of a quagga. Over the next two years, Svante Pääbo confirmed this phenomenon could also be replicated in naturally and artificially mummified human samples up to several thousand years old. While the processes for sequencing ancient DNA were initially laborious, the development of polymerase chain reaction in the late 1980s allowed the field to progress rapidly, though double primer PCR amplification presented issues that were later addressed using a nested multiple primer strategy.

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    © All Rights Reserved
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    The first study of what would come to be called aDNA was conducted in 1984, when Russ Higuchi

    and colleagues at the University of California, Berkeley reported that traces of DNA from a museum
    specimen of the Quagga not only remained in the specimen over 150 years after the death of the
    individual, but could be extracted and sequenced. [5] Over the next two years, through investigations
    into natural and artificially mummified specimens, Svante Pääbo confirmed that this phenomenon
    was not limited to relatively recent museum specimens but could apparently be replicated in a range
    of mummified human samples that dated as far back as several thousand years.[6][7][8]
    The laborious processes that were required at that time to sequence such DNA (through bacterial
    cloning) were an effective brake on the development of the field of ancient DNA (aDNA). However,
    with the development of the Polymerase Chain Reaction (PCR) in the late 1980s, the field began to
    progress rapidly.[9][10] Double primer PCR amplification of aDNA (jumping-PCR) can produce highly
    skewed and non-authentic sequence artifacts. Multiple primer, nested PCR strategy was used to
    overcome those shortcomings.

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