100 % actual size

Otros - Latinoamérica y Caribe “The point of care CD4 provides a result in

+1-877-441-7440 Opción 4
LATAMCustomerSupport@alere.com 20 minutes, is highly correlated with the
laboratory CD4 tests and performed very well
in identifying individuals requiring antiretroviral
therapy. It was highly acceptable among those

Visit AlereHIV.com Alere Pima™ CD4 tested, and may facilitate service delivery in a
Optimising HIV diagnosis and management Reaching Further variety of patient-centred settings /times and
offers rapid assessment of the newly diagnosed
Alere™ HIV Combo HIV patient.”
Now you can help identify HIV just days
after infection.
Dr. Paul Benn, HIV Service Lead
Alere Determine™ Syphilis TP The Mortimer Market Centre, London
Detecting antibodies to Treponema pallidum at
the point-of-care.

Alere Determine™ HBsAg

Detecting surface antigen of Hepatitis B virus
in minutes at the point-of-care.

Alere Determine™ TB LAM Ag

Rapidly screening for tuberculosis in HIV
positive patients with results in just minutes.

© 2012 Alere. All rights reserved. The Alere Logo, Alere, Determine and Pima are trademarks of the Alere group of companies. 12000528-01
 CD4 Testing: The Alere Way Optimise your workflow with Alere Pima™ CD4 The science behind the Alere Pima™ CD4 test Lab quality CD4 results  in your hands

Accurate 34 year old patient newly diagnosed

The Alere Pima CD4 Test generates
™
absolute CD4+ count, equivalent
precision and accuracy as compared
to the lab tests when using venous
draw.¹
Convenient
Compact and portable, the Alere
for a CD4 count
Pima™ CD4 Test provides results
anytime, anywhere.
Patient waits one
The dried reagent sealed within the image capture. T-helper cells are Lab Accurate CD4 Count - anytime, anywhere
HIV positive in the Sexual Health Clinic 1. Collect Sample Alere Pima™ cartridge contains CD3 automatically detected by determining
and CD4 detection markers. Once the where fluorescent signals from both The Alere Pima™ Analyser employs lab based flow cytometry methods.
• Check sample volume
sample is loaded onto the cartridge CD4 and CD3 antibodies are found the same static image analysis and Results show that when comparing
• Remove sample collector
Traditional Alere the reagents are dissolved into the on the same cell. counting principles as existing CD4 the Alere Pima™ CD4 Test to a gold
Pathway Pathway and cap cartridge
blood sample. enumeration technology, in a compact, standard laboratory CD4 Test, the
The co-located signals within the portable and robust package at the Alere Pima CD4 Test provides an
At this time the sample interacts with detection channel are then counted
Blood is taken Patient is tested point-of-care. equivalence of 96 % of all samples that
and sent to lab immediately CD3 and CD4 specific monoclonal and correlated against the volume were tested.3
using the Alere antibodies each labelled with a of the detection channel and the
Thoroughly validated in numerous
Pima™ CD4 test
2. Run Test different fluorescent dye. absolute CD4 count displayed in independent studies the Alere Pima™ 3 Baker, C. K. (2011) Evaluating the Point-of-care
Alere Pima™ CD4 Test with the traditional
Cells / μL right on the analyser screen. Analyser and CD4 test cartridge,
• Insert capped cartridge in the laboratory Flow Cytometer. Northampton General

week Patient receives

The stained sample is then spread consistently delivers results comparable Hospital, NHS Trust
Alere Pima™ CD4 Analyser
CD4 count along the detection channel to await to those obtained using conventional
Flexible within 20
• Result in less than 20 minutes

Requires only a small amount of blood Patient returns to minutes and

allowing for fingerstick or venous whole clinic for result is managed
accordingly
blood samples. Cell labeled with
Red Dye
Conventional CD4 vs Alere Pima™ CD4 3 Bland-Altman 3
2009–09–23 11:30
Using the result
3. Read Screen Result pima cd4 800 + 1.96 SD (+ 138.2)

Difference (Alere Pima™ CD4 – Conventional CD4)

the patient Mean ( - 6.7)

Efficient is managed Sample 67352 Unrecog­nized

cell
1200 y = 9.34E - 01 x + 22.605 r = 0.952 p = 0.000
600 – 1.96 SD ( - 138.2)

It offers a one stop, same-day-result accordingly

• View and read absolute CD3 1000
400

CD4 result CD4 200

Alere Pima™ CD4

for absolute CD4 count in less than BACK PRINT
800
0
Cell with two
20 minutes. 600
antibodies - 200

co-localized 400
- 500
200

1 Innovative Strategies for Providing Enhanced

Research shows a significant proportion No manual gating or software manipulation. - 800
0
Accessibility to Healthcare and Improving HIV of patients recently diagnosed with HIV Cell labeled with
Patient Outcomes. do not attend follow-up appointments Onboard control features ensure result quality. Green Dye
0 200 400 600 800 1000 1200 200 400 600 800 1000 1200

Average CD4 (cells / uL)

2 ‘Evaluation of Pima point-of-care CD4 testing in meaning delays in providing treatment Conventional CD4

a large UK HIV service’ Paul Benn et al, Mortimer

and care.2
Simplistic procedure and easy to follow on-screen instructions. Both charts adapted from: Baker, C.K. 2011
Market Centre, London, 2011