BCC3000 Service Manual 

     
 
          

1
 Content

Content ..............................................................................................................................................

Preface ................................................................................................................................................

Part one Specification ................................................................................................................

Part two Slectrical system ..........................................................................................................

Part three Liquid line....................................................................................................................

Part four Maintenance................................................................................................................

Part five Maintenance Guide......................................................................................................

Part six Mechanical unit disassembly ............................................................................................

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z Preface

Please read the manual carefully in order to maintain the system correctly.

Appropriately keep the service manual somewhere is easily available in order to use it at your
disposal.

Product Name: Auto-Chemistry Hematology Analyzer

Specification model: BCC—3000B

Components: Analysis unit (host), operation unit (computer system), result output unit
(printer), accessories and consumables.

Scope of application: Blood samples for clinical test.

Product registration No.:

Standard No:

Production License No:

Registration Address:

Production Address:

z Reader

Readers of the manual should be Dirui company or the maintenance staff authorized by Dirui.

z Content

This is the service manual of BCC—3000B auto-chemistry hematology analyzer, which

introduces BCC-3000B principle of structure, installation steps, basic theory, maintenance and
repair methods, failure debugging and so on.. Please refer to this manual for maintenance.
z Notes

●The instrument should used only for medical professionals or trained doctors, nurses or

experimentalist.

●Reagent should be placed statically for a period of time until stable.

●Correct handling the waste liquid and instrument consumables is a must, and the blood contained

in waste liquid may have been contaminated by pathogens, therefore, please handle the waste

liquid and instrument consumables according to the regulations coping with medical waste,

infectious waste and industrial waste.

●Samples, quality control, calibrator, waste liquid, etc. possessing potential biological infectious

danger may be pungent to the eyes, skin and mucous membranes. Operator should comply with

the safe operation of the laboratory and wear personal protective equipment (such as laboratory

protective clothing, gloves, etc.) when exposing to the related items in the laboratory.
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●Rinse it immediately with plenty of water once skin or eyes have contacted with the reagent.

Please accept medical treatment if necessary.

●Avoid contacting with the sharp sampling probe which may be appendiculate with blood, quality

control and calibrator having potential biological infectious hazard; in the sampling process, a

certain distance should be maintained between the sampling probe and the bottom of container,

otherwise it will affect the accuracy of the assimilated volume solution. Forasmuch, avoid blood

splash by collision of the tube wall and sampling probe.

●Do not directly contact with a patient's blood specimen.

●Do not repeat using disposable supplies.

●Do not use resin oil, benzene to clean the outside dirt, because it may cause changes in color and

shape. Scrub it with a soft or wet cloth, and clean it with diluted cleaning liquid or alcohol for the

serious.
●Do not use the expired reagent, and forbid dust, dirt, bacteria going in reagent after unseal.

●The outcome of the analysis can not be reliable if the analyzer worked at room temperature

exceeding normal range.

●Do not unplug the printer, bar code scanner at the power-on state.
●Using the specified types of external equipment is must.

●Background check, namely, the analyzer works by using diluent instead of sample diluent.

●The outcome of the analysis can not be reliable if abnormality occurs to the analyzer when it is

implementing background check.

If failure occurs in the initialization process, the corresponding failure information will be
displayed on the screen. The operator should not proceed before solve the failure by referring to
malfunction handling table.

 
 
 
 
 
 
 
 
 
 
 

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Part one Specification

1.1 Name and Model

Name：Auto-Hematology Analyzer

Model：BCC3000B

1.2 Electrical Parameters

Power supply voltage：AC90～240V

Frequency：50/60Hz

Power：less than 200W

1.3 Dimension and Weight

Dimension：

Weight：25Kg

1.4 Performance specifications

1.4.1 Analysis and display range

parameter Display range Linear range Background range
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WBC（10 /L） 0.0~99.9 0.0~99.9 ≤0.3×109/L

RBC（1012/L） 0.00~7.00 0.00~9.99 ≤0.03×1012/L

HGB（g/L） 0~300 0~300 ≤1g/L

MCV（fL） 0.0~300.0

PLT（109/L） 0~999 0~999 ≤10×109/L

1.4.2 Accuracy
The average and measurement error on standard machine should be in the following range with
quality control or calibrator used for testing 10 times.
Whole blood model WBC ±3% or ±0.5×109/L
RBC ±2% or±0.03×1012/L
PLT ±5% or±10×109/L
Dilution model WBC ±5% or±0.4×109/L
RBC ±3% or±0.05×1012/L
PLT ±8% or±15×109/L

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1.4.3 Repeatability
Variation parameter in the 95% confidence interval should be within the following range in 10
times continuous tests by normal fresh blood and QC blood.
1) Whole blood model
Parameter Range Repeatability

(CV%)
WBC 4.0×109/L or more ≤4.0%

RBC 4.0×1012/L or more ≤2.0%

PLT 100×109/L or more ≤5.0%

HGB 0~300g/L ≤2.0%

MCV 80.0~110.0fL ≤2.0%

2) Dilution model
Parameter Range Repeatability (CV%)

WBC 4.0×109/L or more ≤6.0%

RBC 4.0×1012/L or more ≤4.0%
PLT 100×109/L or more ≤9.0%
HGB 0~300g/L ≤2.5%
MCV 80.0~110.0fL ≤3.0%

1.4.4 linearity

Parameter Linear range Linear error

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(1.0-10.0)* 10 /L Less than ±0.5* 109/L
WBC
(10.1-99.9)* 109/L Less than ±5%
(0.30-1.00)* Less than ±0.05* 1012/L
1012/L
RBC
(1.01-7.00)* Less than ±5%
1012/L
(20-70) g/L Less than ±2g/L
HGB
(71-240) g/L Less than ±3%
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(20-100)* 10 /L Less than ±10* 109/L
PLT
(101-999)* 109/L Less than ±10%

1.4.5 Testing speed

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 60 samples/h

1.4.6 Sample volume

Whole blood：13µＬ
Peripheral blood：20µＬ

1.4.7 Dilution ratio

Whole blood: WBC/HGB 1:308 RBC/PLT 1:44,872

Peripheral blood: WBC/HGB 1:428 RBC/PLT 1:43,355

1.4.8 Hemolytic agent volume

Whole blood 0.5mL

Peripheral blood 0.36mL

1.5 Function

1.5.1 Memory

1000 test records、570 QC records

1.5.2 Printer

1） External printer：

2） Internal Printer：

1.5.3 Print item

20 test parameters and 3 histogram；

1.5.4 Serial interface

One for host communication, the other for program escalation;

1.5.5 QC
X Control or L-J Control
1.5.6 Calibration
Manual calibration
Fresh blood calibration
1.6 Reagent

Diluent, cleaning liquid, hemolytic agent, probe cleaning liquid.

1.7 Fuse

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 250v 2A；

1.8 Working condition

Ambient temperature： 15℃~35℃；
Relative humidity： 30%~85%；

Atmospheric pressure： 860 hPa～1060hPa

Installation condition：
Environment should be clean, no mechanical vibration, no substantial noise
source and power supply interference; be not close to brush-type motor,
sparkling fluorescent and regular flashes of electric-contact switch equipment;
avoid direct sunlight or place near sources of heat and wind.
1.9 Storage condition

Temperature: -20℃～+50℃

Relative humidity: <85%

Non-corrosive gases and well-ventilated environment.

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 Part two. Electrical System

2.1 Working principle

Analysis solution

Constant negative pressure

Constant flow source

Voltage
Internal and external electrodes Analysis circuit

Pulse Time

Adopting counting principle of Coulter electrical impedance (see figure 1). Resistance sensor

is constituted by electrodes of one glass gem microporous tube and that of a pair of contraposition

gem microporous tube inside and outside. Constant current is automatically added on electrodes

when testing.

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 A certain amount of electrolyte should be contained in the inspected, namely test can be

carried out when the inspected and diluent resistance ratio of standard particle used by calibrator

must be equal under the specified resistance ratio, which is the condition of resistance test

Resistance method analyzers adopt negative pressure to siphon sample to force the inspected

pass the small cylindrical gem micropore whose inside is filled with the inspected to form the

constant liquid resistance (R0). The increment of resistance ΔR occurs the moment a spherical

standard particle whose diameter is d in the inspected passes the gem micropore because the

resistivity of particle is more than that of the inspected, which is calculated by Coulter formula:

Ρ0──resistivity of the inspected (Ω.cm)

D ── diameter of gem micropore (µm)

d ── diameter of spherical particle (µm)

ΔR is direct proportion to the volume of particle (Volume of sphere ). The output

voltage pulse (ΔV=I0•ΔR) of resistance sensor is direct proportion to the volume of particle as

well. The degree of increased voltage depends on the volume of small aperture sensing zone

taken by non-conductive cells, namely, the bigger the cell are, the bigger the pulse caused are.

The number of cells can be calculated by pulse signal as following steps.

z Amplifying: because the pulse signal generated when passing through the micropore is very

weak so that the counting circuit can not be touched off directly, the pulse signal should be

amplified from micro V class to V class.

z Screening: All kinds of particles can generate corresponding pulse signals when passing

micropore whose level (pulse range) is direct proportion to particle volume. Besides blood

cells and cells in blood, the debris in blood, impurities in diluent all can generate fake signals

which can make counted result higher. Screening means that the fake signals which are lower

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 than reference level should be taken out by using discriminator to improve the accuracy of

counting cells according to the reference level provided by threshold value adjustor.

z Threshold value： Adjust the reference level in the specified scope so that the result of the

calculation probably is in line with the actual value.

z Shaping: The amplified and Screened signal wave shapes of cell pulse differ and need to

be shaped by shaper to be the wave that V is consistent with shape, and which is standard

flat-topped before trigger circuit.

z Counting: The pulse signals of blood cells are sent into the counting system after amplifying,

screening, shaping, and sent collecting peak value of pulse into the CPU simultaneously.

z Overlapping calibration technology

Incorporate the overlapping calibration formula into the instrument testing program by the

instrument logical circuit and math calculation and calculating formula. The instrument can

calibrate result easily after judging and calculating when the situation that two cells pass the

micropore simultaneously.

z Fitting curve technology

To rule out the noise and small red blood cell interference, the accurate counting result of

platelet without noise interference and impact of platelet can be obtained by electronic curve

fitting to count the large platelets which are not counted in accordance with normal volume of

platelet distribution that was log-normal distribution theory.

2.2 Blood cell classification

2.2.1 In the counting of pulse, correctly differentiating erythrocyte, leukocyte, platelet one of key

techniques, which are counted by their size and quantity.

Content of human leukocyte 　 --- 109 / L quantity of class

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 　 The number of human erythrocyte are about 1000 times as many as that of leukocyte, so

counting the number of human leukocyte can be carried out simultaneously when counting

erythrocyte.

z Content of human erythrocyte --- 1012 / L number class

z Content of human leukocyte 　 --- 109 / L quantity of class

z The number of human erythrocyte are about 1000 times as many as that of

leukocyte, so counting the number of human leukocyte can be carried out simultaneously when

counting erythrocyte.

2.2.2 Diameters of erythrocyte, leukocyte, platelet

z Diameter of erythrocyte ------69µm

z Diameter of leukocyte ------7~25µm
z Diameter of platelet ------2~3µm

Working principle：The pulse values of counting cells are different because of different types.

When the threshold value voltage of circuit is V1, start to count erythrocyte and leukocyte,

and the pulse of platelet is screened. The result is the number of erythrocyte. When the

threshold value voltage of circuit is between V2 and V1, the result is the number of platelet.

2.2.3 Leukocyte classification counting

Add hemolytic agent in sample, and erythrocytes are dissolved. Leukocytes are dehydrated

to some extend in the controlled conditions, and the volume of leukocyte depends on the number

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 of tangible substances. Small cells in leukocyte, for instance, lymphocyte is mononuclear cell

with small nuclear, small particles and size of 35 ~ 98fL; large cells in leukocyte, for instance,

neutrophili is polymorphonuclear cell with much particles and size of 160～450fL；However,

mononuclear cell, eosinophil, basophil l, the original cells and late promyelocyte shrink to98～

160fL, which are called middle cell in leukocyte, or median cell. The following shows the

distribution or histogram of leukocyte volume. The different region classification fitting of the

histogram and integral calculating can obtain the count of the leukocyte trisection classification

（LYM#，MON#，GAN#）.

Small cell (lymphocyte) 

Middle cell (mononuclear cell) 

Big cell (neutrophili) 

2.3 HGB test principle

The red blood cell starts to be dissolved after add the hemolytic agent to diluted blood and the

hemoglobin is released, and hemoglobin derivatives are generated after the latter combines

with the hemolytic agent. Enter into the hemoglobin test system and execute colorimetry in a

specific wavelength (usually 530-550nm), and the changes of absorbance is in proportion to

the content of Hb in the liquid, and HGB concentration can be calculated by the instrument.
HGB calculating formula:
Lambert-Beer law

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 K: constant，I0: transmitted light intensity of blank sample
2.4 Volume testing principle

Volume testing principle

 

Start sensor
Start sensor
 

Finish sensor Finish sensor

 

1. Start counting and empty the tube 2. Liquid level descends when

Start sensor Start sensor

 

Finish sensor Finish sensor

 

3. Count starts when liquid level 4. Count finishes when liquid level
passing start sensor passing finish sensor

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2.4 Electrical overall figure

Serial Number Label Name

1 A1 Power supply module

2 A2 Power supply module

3 A3 Power supply board（PCB NO.110703384）

4 A4 Analog board (PCB NO.1107042026 )

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5 A5 Main control board (PCB NO.110703241)

6 A6 Drive board(PCB NO. 110703859 )

7 A7 Display drive board(PCB NO.110700601)

8 A8 Print board (PCB NO.110700764)

9 A9 Rationing board (PCB NO. 110803253)

10 MO1 Diluting motor

11 MO2 Sampling motor

12 MO3 Up-down motor

13 MO4 Rotating motor

14 PHOTO1 Diluting motor light sensor

15 PHOTO2 Sampling motor light sensor

16 PHOTO3 Up-down motor light sensor

17 PHOTO4 Rotating motor light sensor

18 PHOTO5 Rotating motor light sensor

19 PO1 Power line filter

20 P1 Air pump

21 P2 Gas-liquid mixing pump

22 S1 Button switch

23 V1 Three-pass solenoid valve

24 V2 Two-pass solenoid valve

25 V3 Three-pass solenoid valve

26 V4 Three-pass solenoid valve

27 V5 Three-pass solenoid valve

28 V6 Three-pass solenoid valve

29 V7 Two-pass solenoid valve

30 V8 Two-pass solenoid valve

31 V9 Three-pass solenoid valve

32 V10 Two-pass solenoid valve

33 V11 Two-pass solenoid valve

34 V12 Two-pass solenoid valve

35 V13 Two-pass solenoid valve

36 V14 Two-pass solenoid valve

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 37 V15 Two-pass solenoid valve

38 V16 Two-pass solenoid valve

39 V17 Two-pass solenoid valve

40 V18 Two-pass solenoid valve

2.5 Circuit board

1、Main control board
CPU circuit board consists of the display module, A / D module circuit, I / O module circuit,
which controls the display of the entire system, data collection, data storage and internal and
external printing. The main control part of hardware mainly includes LPC2214, and the peripheral
device adopts flash memory SST39VF1601, SST39VF200A, AT45DB642D serial FLASH for
data expansion, IS61LV25616AL RAM, PCF8563 for clock chip, MAX232 for interface chip,
CycloneIIEP2C20Q240 FPGA, EMP240-100 CPLD chip for functional expansion, AD7928
AD7918 A / D chip for data collection. LPC2214 achieves human-computer interaction by
controlling the LCD screen, touch screen and printer, serial interface, and completes all kinds of
control function by controlling the FPGA. FPGA EP2C20 gets the control information through
LPC2214 interface, and the issued ARM instruction is accomplished by the built-in NIOS
soft-core and other logic circuits. CPLD mainly controls I / O extension function.

17
 Real  time  Internal 
clock  printer 
Serial flash 

Drive 
board 
interface  LCD screen 

Touch screen 
Liquid level sensor

Control interface of amplification board 

Data collecting model

RS232 interface

External printer

Main board frame 

J1 Rationing board interface J8 Switch controlling interface
J3 Drive board interface（communicate with drive J13 Display board interface
board，controlling pumps, valves）
J5 Print board interface P1 Parallel interface, serial interface
J6 Analog board interface J4 Liquid level sensor interface
J7 Power supply interface（+5v）

1.1 Layout

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2、Drive board
Power drive board carries out controlling deflection motor, up-down motor and injector motor
according to the instruction of upper machine, and controls pumps and valves by receiving the
switch signal of upper machine. Power mainly consists of three models: switch control module,
motor control module and power module. Power supply 

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 Power supply 

Detection  module  of  light 

sensor 

Drive module of solenoid 
valve 

Drive module of motor
Serial port 

Mainboard interface 

Drive module

J1 Interface connecting to main board

J2 Valve control line interface
J4 Air pump interface
J6 Power interface (+24 v)
J7 Diluting motor control interface
J8 Sampling motor control interface
J9 Rotating motor control interface
J10 up-down motor control interface

2.1 Layout

20
2、 Analog board

21
 Analog board is reasonable for changing original signal into the signal which meets the A/D
conversion requirement, including WBC and RBC + PLT amplification unit, HGB amplification
unit, the temperature measurement unit, vacuum measurement unit, power supply monitoring unit,
processing unit of small hole voltage, electronic analog switch module etc..

Temperature sensor 
温度传感器
Voltage check  电压检测

检波波形
Check wave start point
Amplifier  Amplifier  起 点
放大器 放大器 Sensitivity
灵敏度increasement
增 益

Absorbance 
WBC
吸光度 Main control board 
主控板
Converting 
HGB 放大器
Amplifier  放大器
Amplifier  模数转换

RBC
检波波形
RBC Check
起 wave
点 start point
放大器
Amplifier 
放大器
Amplifier 
灵敏度
Sensitivity increasement
增 益
100V  voltage  Amplifier 
放大器
100V电压电路
circuit   

Check wave start

检波波形
PLT point
Pressure sensor  Amplifier  起 点
压力传感器 放大器 Sensitivity
放大器 灵敏度
Voltage check 
电压检测 Amplifier  增 益
increasement

模拟检测模块原理框图
Principle frame of simulation check module 

J1 RBC transducer interface J3 Power interface（+/-12v）

J2 WBC transducer interface
J4 HGB interface
J6 Temperature sensor interface
JC Main board Interface

22
3.1 Layout

23
4、Volume computation
Volume computation board, by detecting the liquid volume with the light sensor of
computation tube, ensures the WBC and RBC + PLT test accuracy.
4.1 Layout

24
5、Display board
Control the interface through the main board.
5.1 Layout

25
6、Printing board
Singlechip LPC2114 on the board communicates with the main control board CPU through
the serial port, and printing of characters and waveforms and sending real-time status of counter to

26
the host CPU to display is achieved in accordance with the instruction of main control board CPU
with total two sets power supply on the board. Printing head heating voltage is provided with the
voltage that has been converted from 24V DC voltage, and CPU power supply is provided with
5V by the main board.

Power  supply  of  JTAG  adjusting 

数字电路电源
digital circuit  Micro  JTAG调试接口
interface 
微处处理器
processor 

串行数据输入
Serial  data 
input 

Motor  drive 
Heater  of  paper‐in 
Power  打印头加热器 进纸电机电机驱
打印机电源
电路 of 
circuit  motor 动
supply  of 
print head 
printer 

Figure Circuit frame of printer 
图 1: 打印机电路框图

JP7 Main board interface

JP8 Power supply interface

27
6.1 Layout

28
7. Power supply board
Providing system power supply:+5v，±12v。
7.1 Layout

J1 +5v power supply

J2 Power inlet
J3 ±12v power supply（Driver board）

29
Part three Liquid line

1.1 Working principle

In the whole blood mode, diluted sample of ratio about 1:269 is formed by mixing 13ul of

whole blood sample and 3.5mL of diluent that are assimilated by counter, which is divided into

two parts. Counting sample of ratio about 1:44872, obtained by mixing the 15.6ul diluted sample

and 2.6mL diluent, is used for counting erythrocyte and platelet; counting sample of ratio about

1:308, obtained by mixing the rest of diluted sample and 0.5mL hemolytic agent, is used for

measuring the concentration of hemoglobin and counting leukocyte.

              13μL whole blood sample 

3.5 mL diluent  

Sample of diluting ratio about 1：269 
全血工作模式稀释流程  

0.5 mL hemolytic agent 

About 2.6 mL diluent 

Leukocyte  counting  sample  of  Erythrocyte/platelet  counting  sample  of 

diluting ratio about1：1：308    diluting ratio about 1：44872     

1.2 Pre-diluting mode

In the pre-diluting mode, diluted sample of ratio about 1:36 is formed by mixing 20ul of

peripheral blood sample and 0.7mL of diluent (diluted externally). Diluted sample of ratio about

1:384, obtained by mixing 0.3mL previous diluted sample with 2.9ml diluent that is assimilated by

counter, is divided into two parts. Counting sample of ratio about 1:43355, obtained by mixing the

24.8ul diluted sample and 2.8mL diluent, is used for counting erythrocyte and platelet; counting

sample of ratio about 1:428, obtained by mixing the rest of the previous diluted sample and

0.36mL hemolytic agent, is used for measuring the concentration of hemoglobin and counting
30
leukocyte.

20μL peripheral blood sample   

0.7mL diluent 

Sample of diluting ratio about 1：36   

2.9mL diluent 
Sample  of  diluting  ratio 
about 1：384 

0.36mLhemolytic agent  About 2.8mL diluent 

Leukocyte  counting  sample  of  Erythrocyte/platelet  counting  sample  of 

diluting ratio about11：428    diluting ratio about 1：43355     

Flow of pre-diluting mode

1.2 Assembly of liquid line

31
  

2 

20 3 4

6 5 7

8 
9 

 
10

14
11  12

13
15

16
21

17 

18 19

1,10:    Buffering bottle 
2:          Liquid absorbing pump 
3,4,6,7: light sensor 
5,8:        Flowmeter 
9:          Cleaning liquid bottle 
11:        Hemolysin bottle 

32
12:        Positive pressure pump 
13:        Pressure sensor 
14:        Hemolysin pump 
15:        Sampling pump 
16:        Diluting pump 
17:        Diluent pump 
18:        Sampling probe 
19:        Rinsing bath 
20:        Hydraulic pump 
21:        Pressure pump 

33
Part five. Maintenance

4.1 System status

The information and the normal range of system status, such as ambient temperature, counter
HGB zero voltage, HGB background voltage, power supply voltage, vacuum chamber pressure
and the pressure chamber pressure, are displayed on the main interface.
     ~ system status ~ 
 
         
Item                                                          Detection value                                    Range 
ambient temperature                                  26.0                        15.0‐30.0 
HGB zero voltage                                      0.00                        0.00‐0.20 
HGB background voltage                        1.8                          1.40‐2.50 
DC    +12V                                                  11.71                      11.04‐12.96 
DC    ‐12V                                                11.84                      ‐11.04‐‐12.96 
106V(V)                                                          108                        100‐112 
56 V(V)                                                            56.40                    53.00‐59.00 
Vacuum chamber vacuum HP                    201                      175‐205 
pressure chamber pressure HP                67                        40‐70 
 
                                          
                                                   
Test Exit

Figure 3-1
4.2 Valve check
Valve switch can be checked whether it is normal in the valve interface. System liquid lines
will not work normally if the valve fails.
Steps are as follows:
Press V1~V18 to start check before select the items “V1”、“V2”、“V3”……“V18” if the “OFF”
is shown in the corresponding result column of items. After check, the status key shows as “ON”.
It can be inferred preliminarily that valve works normally if the sound that valve and switch issue
sounds normally in the process of checking. Contrarily, failure may happen to valve.

    ~Valve Check~
 
    Valve            Valve            Valve            Valve          Valve 
  
V1    OFF      V5      OFF      V9    OFF        V13    OFF  V17    OFF 
 
  
V2    OFF      V6      OFF      V10    OFF      V14    OFF  V18    OFF 
 
 
V3    OFF      V7      OFF      V11    OFF      V15    OFF                   
 
 
V4    OFF      V8      OFF      V12    OFF      V16    OFF                 
  Figure3-2
Confirm                                                                    Exit 

Figure 3-2

34
4.3、Description and use of menu of instrument system check and diagnosis
Total 19 tests, such as counter hardware, machinery parts and liquid line components, are
tested by system, and it is shown that whether the component is in normal state or whether the
time of the test is in the range of time.
System testing is fluid path for a total of 19 tests showed or to the time required for this operation.
Check whether 2.5ml and 50ul, 10ml, deflection and up-down motors run normally in all
directions.

System  Testing

No. Item Result No. Item Result 

1 WBC A/D status 11 Sampling motor
2 RBC A/D status 12 10ml motor
3 PLT A/D status 13 Deflexion motor
4 WBC A/D intermitted 14 Up-down motor
5 RBC A/D intermitted 15 Printing
6 PLT A/D intermitted 16 Vacuum of vacuum chamber
7 WBC path timing 17 Pressure of vacuum chamber
8 RBC path timing 18 Pressure of pressure chamber
9 WBC ventage voltage 19  Filter of vacuum chamber 
10 RBC ventage voltage
   

Confirm  Up  Down  Exit 

Figure3-3
1．Press 【Down】to move icon to any item, press 【Confirm】key, system starts to test item chip.

2. If the result is normal, “Normal”(Y) will be displayed in the corresponding result column of the

"Test Item" .

3．If test results are abnormal, failure (N) will be displayed in the corresponding result column of

the "Test Item", and refer to malfunction at this time or measure the test points of relative valtage.

4. Press 【Exit】 to exit the system monitoring interface.

4.4 Maintenance

4.4.1 Regular maintenance

Daily 1, if instrument is not shut down for 24-hour, conducting "counting cell

cleaning" daily is a must (not less than 40 minutes immersing is

recommended) or press [Rinse] button to carry out the rinsing operation

2、QC
35
Every 3 days if instrument is not shut down for 24-hour, conduct "Immerse probe top"

every 3 days is a must.

Weekly If instrument is shut down normally every day, conduct "Immerse probe

top" weekly

Monthly Calibrate sampling probe with sampling probe height adjustor

4.4.2 Maintenance when needed

● If the counting cell is contaminated, implement “Counting cell cleansing " operation;

● When the counter is not used for more than two weeks, implement " Exhaust liquid line"

operation in "System maintenance" menu to complete the counter liquid line exhausting and

cleaning, and wipe counter and place it in a clean site;

Press 【Eliminate block】 key manually or execute “Burn gem hole” and “Back-flush gem hole”

operation.

● Reliable analysis result can be obtained when the counter works in a normal circumstance. So,

counter working status should be confirmed by implementing “System check” operation.

4.5 Method
10 ways are available in the system maintenance interface

    ～System maintenance～                                     

 
1. Replace diluent                                6. Cleaning liquid immersion probe   
2. Replace cleaning liquid                    7. Cleansing pool count   
3.Replace hemolytic agent                  8. Cleansing swab   
4. Burn gem hole.                                  9, Exhaust liquid line 
5. Back flush gem hole                        10. Exhaust count pool   

36
Part five Maintenance Guide

Initially, it is needed to check whether the wiring of instrument is loose and the connecting of
liquid lines, pipelines of diluent, cleanser and waste liquid is good. Check system status
instrumentally and systematically check relevant function when start the instrument.

1. Boot
No. Function Malfunction Solution
1 Displaying Displaying 1. Adjust backlighting potentiometer
“loading…” failure 2. Check whether the LCD connection is loose
3. 3. Rewrite BOOT program
2 Entering self-check Displaying 1. Re-weld mainboard ARM chip and FPGA chip
interface failure 2. Rewrite ARM main program
3. Replace mainborad
3 Motor failure 1. Check whether the corresponding light sensor is
normal
2. Check whether the motor wire and light sensor
wire are loose.
4 System status Status 1. Check analog board
abnormal 2. Check whether the wire of temperature sensor is
loose.
5 System check Corresponding Replace mainborad EPCS4
AD status
normal, break
error
6 Sampling volume Sampling 1. Check whether the corresponding injection pump
check volume error is loose
2. Check whether there are bubbles or leakage to
corresponding pipeline
7 Blank check PLT test*** 1. Check whether poor soldering exists at RBC
sensor connecting wire
2. Exchange RBC sensor and WBC sensor
connecting wire
Replace the RBC cup body if WBC counting
abnormal
3.Replace analog board
8 PLT bland pass Replace diluent
failure
9 WBC bland
pass failure
10 Histogram-out Histogram setup option failure
failure

37
11 Sampling probe 1. check 2 # pipeline
leaking 2. check 4 # pipeline
12

2. Maintenance in testing

No. Function Malfunction Solution

1 HGB background 1、 Whether Liquid flows into
voltage low HGB colorimetric unit
2、 Whether the installation of
colorimetric unit is correct
3、 Whether WBC cup body is
broken
4、 LED fails to shine
5、 Analog board failure
2 Overtime ration 1、 Hole blocked
2、 Ration board light sensor
does not shine
3、 Connection point of ration
tube leaks air
4、 Check 8# and 18# valves if it
happens repeatedly.
5、No reagent, outside
pipeline blocked (pressed),
inside pipeline blocked
(folded), cell and pipeline
leak air, valve failure.
3 Poor test 1, Remove shield of cup
repeatability body, and check the bubble
mixing
2, the pressure is incorrect
4 WBC 1, check whether the ground
background value wire inside the machine is loose.
high 2, watch the pulse
histogram displayed
3, use probe cleaning liquid
to immerse counting cell
4, open the front door to
count exclude interference of
circuit.
5, check whether there are
bubbles in 10mL syringe
(peripheral blood).
Dismount colorimetric unit
and mount it after wipe it.
Remount
Replace cup body
LED connecting wire broken,
re-weld it.
Check analog board failure
Implement burning, back
flushing program.
Adjust corresponding
potentiometer.
Reconnect or replace silicone
tube.
Disconnect the corresponding
valves and clean the
membrane
Disconnect gem hole and
clean it.
Clean the pipeline connected
to cup body
1、 adjust potentiometer VR4
2、 replace analog board

38
 6, open, clean the front and
back counting cells of counter and
the gem hole, and replace
microporous gasket.
7, Cut off the inflow of
hemolysin to exclude hemolysin
interference.
8,Check the lower
connected pipes of counting cell,
rinse the lower pipeline if it is Repair analog board
dirty.
9, valve dirty, not tightly
closed, poor opening.
10, replace counting cell.
11, analog board broken failure
5 PLT background 1、 whether the background Replace reagent
value high value is stable
2、 whether there is ground wire
3、 watch the histogram
4、 use probe cleaning liquid to
immerse counting cell
5、 open, clean the front and
back counting cells of
counter and the gem hole,
and replace microporous
gasket.
6、 Pipeline on the surface of Replace cell and check analog
cell is dirty and rinse it. board
7、 Counting cell can’t be
emptied; V11 valve dirty,
not tightly closed, poor
opening.
8、 Electrode of count failure
9、 Analog board

6 WBC blood 1, whether the background is

sample value low normal
2,whether calibrating
coefficients are correct,
3, whether there are
hemolysin, check hemolysin
adding volume; check 2.5mL
syringe and related valves
4, valve dirty, valves

39
 broken.
5, analog board
6, HGB adjustment alarm
7, Watch the background of
HGB voltage
8, immerse, clean WBC
cell
7 HGB background 1, clean WBC cell if it has
voltage not stable dirt.
2, whether there is water
membrane between the outside of
cell and HGB rack.
3, HGB illuminates and
receives connection oxidation
4, analog board
8 RBC、PLT blood 1, the cell is not emptied,
sample value low and poor valve opening.
2, whether sampling needle
is sampling and diluting normally.
3, analog board
4, whether sample
assimilating is normal and re-set
it height
9 WBC blood 1, the cell is not emptied,
sample value low and poor valve opening. (RBC
value low at the same time)
2, sampling probe blocked,
sampling volume inadequate RBC
value low(at the same time)
3, leakage occurs between
sampling probe and 10mL
syringe (RBC value low at the
same time)
10 WBC/RBC Bubbles simultaneously
bubble 1, check whether there is diluent
2, check whether there is diluent
in the two cells.
3, check whether the 10mL
syringe is filled with liquid; if not,
leakage occurs in 10mL syringe
or pipeline.
4,10ml syringe upper pipeline is
kinked, and pipeline is blocked
(10mL motor issues failure at the

40
 same time).
Bubbles singly
1, back cell leaks
2, valve membrane broken
3, pipeline leaks
4,valve is not closed tightly or
valve membrane is broken.
11 when counting 1, Diluent and rinsing liquid
blood sample, are connected in reverse.
WBC value is 2, vacuum degree is low
normal; RBC 3, waste liquid pipe is
value is less than kinked and liquid exhausting is
1; PLT is 0 or a poor.
few dozen or 4, vacuum filter is blocked
normal 5, vacuum pump failure
6, valve is closed tightly.
7, vacuum tank and
relevant pipelines leak
8, vacuum sensor of analog
board are crystallized and
blocked.
12 Motor failure 1, the relevant line blocked
2, motor broken
3, light sensor broken
4, drive board failure
13 Hard to classify 1, what’s the WBC total number
WBC 2, check the background and
eliminate its interference factors
3, check whether the histogram is
compressed
4, what’s the voltage of small
hole
5, check whether it is diluent of
the original factory or it is
expired.
6, check whether it is whole
blood or peripheral blood, and
whether anticoagulant ratio is
followed EDTA K2
7, check whether specimen is
from children and specimen
storage time

3. MCV value Not matched diluent

41
 high or low

3. Malfunction Code

Malfunction Malfunction
No. Malfunction Code Solution
description cause
1. Press “confirm” button
to reset motor
2. Check the diluting pump
32—41、48、49、 Diluting motor
Diluting motor motor
53—55、 61、 62、 abnormal with
1 failure 3. Check driver board
64、65、66、 abnormal sound
4. Check driver board power
187—189
supply
5. check LCP2131
6.Replace driver chip 3977
1. Press “confirm” button
to reset motor
Sample motor 2. Check the sample motor
Sample motor
16—22、24、 abnormal with 3. Check driver board
2 failure
25—28、179、185 abnormal sound 4. Check driver board power
supply
5. check LCP2131
6.Replace driver chip 3977
1. Press “confirm” button
to reset motor
2. Check the rotating motor
Rotating motor Rotating motor
3. check light sensor
abnormal with failure or light
3 112、115、116 4Check driver board
abnormal sound sensor is not
5. Check driver board power
checked
supply
6. check LCP2131
7.Replace driver chip 3977
1. Press “confirm” button
to reset motor
2. Check the s up-down
Up-down motor Up-down motor
motor
50、113、114、117、 abnormal with failure or light
4 3. Check light sensor
118 abnormal sound sensor is not
4. Check driver board power
checked
supply
5. check LCP2131
6.Replace driver chip 3977
5 176—178、184、186 Clean swab; motor failure 1. Press “confirm” button

42
 automatically reset to reset motor
if not assimilating 2. Check the sample and
sample diluting pump motor
3. Check driver board
4. Check driver board power
supply
5. check LCP2131
6.Replace driver chip 3977
1 Check the analog board
Reset signal and mainboard cable.
6 180—183 Circuit failure
abnormal 2. Check the analog board
and mainboard
1. Check liquid line
2. Check analog board
3. Check +12 V voltage
4. Replace U19
5,Replace potentiometer
Liquid line and VR negative pressure and
7 2—5 Pressure abnormal analog board VR3 positive pressure
failure 6.Replace pressure sensor
negative pressure U21 and
U23 positive pressure
7.Replace U14 negative
pressure and U15 positive
pressure
1, Replace diluent,
cleaning liquid in the
maintenance interface
2, Check HGB background
voltage
3, check white blood cell
Liquid line counting cell, and check the
bubbles, relevant pipeline and
counting cell solenoid valves （3、1、4、
8 201 HGB Blank error cracks, bad light 5）if there is no liquid.
penetrating, bad 4, Check± 12V voltage
colorimetric (corresponding failure 202)
terminals 5, check the colorimetric
terminal
6, check the 100V voltage
and 56V Voltage
7, check Q14 (IRF210)
8, check U32 (UC3438)
9, check U12

43
 1. Check analog board and
mainboard
2. Check the cable between
56v failure, the analog board and mainboard
lower probe, 3.Observe analog board
9 202 temperature alarm Circuit failure indicator
issues when probe 4. check ± 12V power
up and down supply voltage
5. Check 100 voltage,
6. check ZD1
7. Check 56V voltage
1. check whether the lead
of ration tube and its light
sensor is damaged
2. Check light sensors P1,
P2, P3, P4
Ration tube
3.Check potentiometers
10 203 Ration tube failure light sensor
VR1, VR2, VR3, VR4
failure
4. Check U2A, U2B, U2C,
U2D
5. Check whether solenoid
valves 7,17,8,18 are normal.
6. Check driver board
1,Implement washing,
1. Erythrocyte
immersing, eliminating
test unit hole
Counting cell liquid block, burning, back flush
blocked
Ration overtime overflows, and it is and other maintenance
11 2. leukocyte test
issued that ration is measures
unit hole
over time 2, clean gem hole
blocked
3, Clean No. 2,10,11,12
3.valve blocked
valves.

44
Part SIX Mechanical Unit Disassembly

Disassembly of counting cell and its components

3 2
5 
Gem hole 

Rubber pad 
6 

4 

Figure 1
1、Setscrew 2、Transducer 3、Vacuum chamber
4、Ruby (microporous) 5、Mesoporous tube 6、Rubber gasket

1.1 Installation of leukocyte transducer

Components of vacuum chamber

Figure 2
Install the fluorine rubber gasket, gem microporous 100 (mind direction) and vacuum chamber
components into the transducer, and lock them tightly on the transducer by self-tapping screws;
2、Disassembly and component of HGB Rack

45
 HGB rack on WBC count pool 

Figure 3

HGB rack installation hole 

Figure 4
Install LED component, optoelectronic receiving tubes into the O-ring into the transducer, and
lock them tightly by pan head screws.

3、Disassembly and component of sampling unit

3.1、Installation of rotating and up-down motor components
5 
8 
6 
1 

2  4 
3 
7 
Cable 21 

Cable 20 
Illustration

46
1、Guide closing tube 2、Cross pan head screw 3、Bracket of rotating motor 4、Step motor
5、Cross pan head screw 6、Cross pan head screw
7、Linear motor 8 Bracket of up-down motor

1)、Paint the screw thread on cross pan head screw with screw thread fastening agent, lock the step
motor（cable 21) tightly on the bracket component of the rotating motor with screws, and fix the
guide closing tube on the bracket of rotating motor with cross pan head screw as the following
figures shows, which assembles rotating motor components.
2)、Paint the screw thread on cross pan head screw with screw thread fastening agent, lock the
Linear motor（cable 20) tightly on the bracket component of the up-down motor with screws
（Note：lifting lead screw is forbidden when moving motor）as the following figures shows, which
assembles up-down motor components. Note: Sequently, put the components on table with
leadscrew up and motor down
3.2 Installation of components of connecting items, slider and rotating arm

4 10

1 
9 
3  5 

8 
2 
6 
7 

Illustration
1、Connecting item 2、Cross countersunk head screw 3、Swab bracket 4、Cross pan
head screw 5、flange board of screw nut 6、N
7、Up-down light sensor 8、Cross countersunk head screw 9、Up-down slider 10、swaying
arm of sampling probe

1)、fix the swab bracket on the connecting item with the Cross countersunk head screw （2）to
assemble connecting items as the following figure shows；
2)、①Buckle the up-down slider and swaying arm of sample probe as the direction of figure 4
shows. (Note: the trough of sampling probe swaying arm should be buckled down to the bottom of
the slider circular button)
②fix the flange board of screw nut on the swaying arm of sampling probe with the cross
countersunk head screw(4).
③fix the nut on the up-down slider with the cross pan head screw（8）, and fix up-down light
sensor on the up-down slider with cross pan head screw to assemble the slider and the components
of swaying arm .

47
3.3 Installation of screw nut components, up-down motor and connecting components.

3
2 

4 

5  6 Cable 20 

Illustration

1、Rotating light sensor 2、Screw nut（spline shaft） 3、Deep groove ball Bearing
4、Up-down motor component 5、Interspace connection board 6、Internal hexagon head screw

1)、Install deep groove ball Bearing at the short end of optical axis of screw nut (bearing is
supposed to be install at the bottom of short end of optical axis), assemble rotating light sensor
on the screw nut to form the screw nut components as figure shows:
2)、Paint the screw thread on hexagon countersunk head screw with screw thread fastening agent,
fix the bracket of up-down motor on middle connection board to form the up-down motor and
middle connection components

48
3.4  Component installation and component connection of rotating motor and supporting
components:

1 

3  4 
2 

7
5 

Illustration
1、Slider and swaying components 2、Screw nut components
3、Linear axis 4、Rotating motor and middle connection components 5、Flange outer ball bearing
6、Rotation support 7、Rotating motor components

1)、Install the linear axis and screw nut components on up-down motor board, and install slider
and swaying components on the screw rod of up-down motor as figure shows:
2)、Install flange outer ball bearing on the screw rod of up-down motor, and install rotation support
on the axis end of step motor to form rotating motor and supporting components as figure shows:

49
3.5 Overall assembly

 
 
0.2 
 
                    
 
        
   
  
 
    Cable 21  Cable 20 
   
     
     
                                 
 
  1 
 
 
2 
 
  3
  Cable 21 
 
 
 
                                                                Illustration 
 
1 Connection components 2 、 Rotating motor and supporting components
3、Hexagon countersunk screw 4、Screw thread fastening agent
Assemble the connection components on the long end of screw nut, install the up-down motor
screw rod and one end of linear axis into the corresponding holes of rotating motor board,
adjust the parallelism of rotating motor board and up-down motor board to make sure the
difference of the two boards is less than 0.2mm, paint the screw thread on the hexagon
countersunk screw with fastening agent and fix the rotating motor bracket on the middle
connection board as figure 9 shows:

Note: after completion of installation, move the slider manually to make sure it moves smoothly.

50
1.Disassembly and structure of liquid pressure unit
4.1 Installation of left and right block

1  7 6

2  3 
4 5
illustration
1、Friction sleeve 2、Cross countersunk screw 3、Cross pan head screw 4、Light sensor
5、Shaft ring 6、Linear axis 7、Left block

1，Assemble linear bearing into left block, fix it with shaft ring
2，Lock the friction sleeve tightly with cross countersunk screw on the right block.
3，Lock the light sensor on the left block with pan head screw as figure shows:
4, Ibid to the right block

4.2 Installation of motor components

1  4

2 
Cable 23  Cable 22 
3 

Illustration
1、Cross pan head screw 2、Motor seal sleeve 3、Linear motor 4、Lower board

51
 Assemble the motor seal sleeve into lower board, with whose top end pointing at the middle
area of upper and lower board, assemble the Linear motor into the lower board and lock it
tightly with pan head screw to form motor components with the cable coming out direction as
figure shows:

4.3 Installation of middle connection board and upper and lower boards

1 

2 
3 4

Illustration
Adjust the distance of upper and lower boards, and lock the upper and lower boards tightly at the
screw holes of both ends of middle connection board after paint hexagon column head screw with
screw thread fastening agent as figure shows:

4.4 Overall assembly

1 

2  3  4  5 6
Illustration

1 、Linear bearing rod 2、Middle rod 3、Motor screw rod

4、MSSU4-10 5、Cylindrical pin 6、MSSU4-6

52
1, Install the middle rod into the middle, lock it tightly after paint it with screw thread fastening
agent MSSU4-6, assemble the linear bearing rod into the left and right blocks respectively, rotate
into the corresponding positions of upper and lower boards and lock them tightly after paint them
with screw thread fastening agent 2-MSSU4-6, and keep the end levels of the three rods and
outside end of upper board at the same level.
2、Tighten the screw rod in the left block and right block after push it through the motor seal
sleeve, and lock the motor axis tightly on the left and right blocks with 2 MSSU4-10 hexagon set
screws with chamfered end , which is painted screw thread fastening agent.
3 、 Insert 3 cylindrical pin into corresponding positions of hydraulic connector, hydraulic
connector1 as figure shows;
 
Overall assembly 2：

2  3  4  5  6 

7 8  9 

Illustration
1、2.5ml injection pump 2、Screw 3、50ul injection pump 4、10ml injection pump
5 、 injection pump interface 1 6 、 Hydraulic interface 7 、 Baffle 1
8、Baffle 9、Cross pan head screw

1, Tighten injection pump interface 1 on the 50ul injection pump, spread the glue 704 evenly on
the injection pump and the connecting point of connector. The same way to install the hydraulic

53
connector the other two injection pumps and they are available until glue is congealed.
2、Install the end of 3 injection pump components intoФ3 pin hole, and lock the other end on the
left and right blocks with 3 stainless steel screws as figure shows; Note: It is forbidden to shake
the 50ul injection when pulling down the left and right blocks to motor.
3、Lock the baffle 1 and baffle on the upper board with 4-M3x8 pan head screw as figure shows:

54