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BCC3000
Automatic Hematology Analyzer
 Contents

BCC3000 Working Principle

BCC3000 Performance Features

Composition

Maintenance
BCC3000 Working Principle
WBC/RBC / PLT Testing- Impedance Method
Electrical Impedance Method is based on
the measurement of changes in electrical
resistance produced by a particle passing
through an aperture.
As blood cells are poor conductive, when
they pass through the aperture, they
cause an increased impedance, the
impedance directly proportioned to the cell
size.
Working principle
Size of Cells
An important feature of the electrical pulses is the relationship of
the pulse height to the size of the cell passing through the
aperture. Larger particles or cells generate higher pulse
amplitudes. Fortunately, each cell type falls within a predictable
range of sizes making it possible to distinguish one type of cells
from another. During cells sensing period, approximate normal
size ranges for the different cells are:
WBC’ s: 30-400 femtoliter
RBC’ s: 30-250 femtoliter
PLT’ s: 2-25 femtoliter
The number of pulses is equivalent to the cell number
through the aperture.

The pulse amplitude is proportional to the cell size.

All electrical pulse is classified according to different

channel voltage value.

Reagent ：
Diluent ： diluting, rinsing, offering conductive
environment, cells equal volume processing
Quantitative tube
Hemoglobin Concentration Testing-Colorimetry
 The diluted sample is mixed with lyse reagent, RBC dissolve, releas
ing hemoglobin.
 Hemoglobin combined with lyse reagent to form hemoglobin complex.
 The hemoglobin complex is irradiated by monochromatic light with a w
avelength of 546nm .
 Phototube is used to receive transmission light, and convert the light sig
nal into voltage signal.
 By comparing with the voltage produced by background transmission li
ght intensity before sample adding (only diluent exists), the hemoglobin
concentration can be obtained(HGB), unit is g ／ L.

HGB=Constant × Ln background transmission light intensity

sample transmission light intensity
Reagent consumption
Name When switch on When When When runs
power off runs （ whole ） （ predilute
d）
cleanser 32ml 20 11.2ml 11.2ml

Lyse 0 0 500ul 500ul

diluent 61ml 0ml 27.5ml 28.2ml

B-Z cleanser 0 650ul 0 0

probe cleanser 0 0 620ul 0

diluent 64ML.cleaning solution 17.5ml when rinsing pipeline system

BCC3000 Performance Features
Sample Volume : Prediluted 20ul
Whole Blood 13ul
Aperture Diameter : WBC 100um
RBC: 70um
Wavelength : 540 nm
Throughput : 60 samples per hour

Dilution rations :
Whole Blood Capillary Blood
WBC/HGB 1:308 1:428
RBC/PLT 1:44872 1:43355
Lyse Volume Whole Blood Capillary Blood
0.5 ml 0.36 ml
Parameters

White Blood Cell or leukocyte WBC

Lymphocyte LYM#
Mid-sized cell MON#
Granulocyte MXD#
Lymphocyte percentage LYM%
Mid-sized cell percentage MON%
Granulocyte percentage MXD%
Red Blood Cell or erythrocyte RBC
Hemoglobin Concentration HGB
Mean Corpuscular (erythrocyte) Volume MCV
Mean Cell (erythrocyte) Hemoglobin MCH
Mean Cell (erythrocyte) Hemoglobin Concentration MCHC
Red Blood Cell (erythrocyte) Distribution Width Coefficient of Varia
tio RDW-CV
Red Blood Cell Distribution Width Standard Deviation RDW-SD

Hematocrit HCT
Platelet PLT
Mean Platelet Volume MPV
Platelet Distribution Width PDW
Plateletcrit PCT
Platelet Larger cell ratio P-LCR
WBC Histogram
RBC Histogram
PLT Histogram
Storage: 1000 testing results, 570 QC results
Language: English
Operation Environment:
Temperature ： 15℃ ～ 30℃ ；
Humidity ： 30 ％～ 85 ％；
Air Pressure ： 76kPa ～ 106kPa
Input/Output: RS-232, Internal thermal printer, 1 bar code reader (optional),
1 parallel printer(optional)
Data transmitting rate: 19200bps
Power supply: 100~240VAC ， 50/60Hz
Fuse: 250V 2A
Power: 200W
Weight: 25Kgs
Dimension: 460(L)×390(W)×410(H)
Analysis and display range
parameter Display range Linear range Background range
WBC （ 109/ 0.0~99.9 0.0~99.9 ≤0.3×109/L
L）
RBC （ 1012/ 0.00~9.99 0.00~7.00 ≤0.03×1012/L
L）
HGB （ g/ 0~300 0~300 ≤1g/L
L）
MCV （ fL ） 0.0~300.0 　 　

PLT （ 109/ 0~999 0~999 ≤10×109/L

L）
Accuracy

Whole blood model WBC ±3% or ±0.5×109/L

RBC ±2% or±0.03×1012/L

PLT ±5% or±10×109/L

Dilution model WBC ±5% or±0.4×109/L

RBC ±3% or±0.05×1012/L

PLT ±8% or±15×109/L

 Repeatability
Variation parameter in the 95% confidence interval should be within the following
range in 10 times continuous tests by normal fresh blood and QC blood.
1.Whole blood model

Parameter Range Repeatability

(CV%)

WBC 4.0×109/L or more ≤4.0%

RBC 4.0×1012/L or more ≤2.0%

PLT 100×109/L or more ≤5.0%
HGB 0~300g/L ≤2.0%

MCV 80.0~110.0fL ≤2.0%

2.Dilution model

Parameter Range Repeatability (CV%)

WBC 4.0×109/L or more ≤6.0%

RBC 4.0×1012/L or more ≤4.0%

PLT 100×109/L or more ≤9.0%

HGB 0~300g/L ≤2.5%

MCV 80.0~110.0fL ≤3.0%

linearity
Parameter Linear range Linear error
(1.0-10.0)* 109/L Less than ±0.5* 109/L
WBC
(10.1-99.9)* 109/L Less than ±5%

(0.30-1.00)* 1012/L Less than ±0.05* 1012/L

RBC
(1.01-7.00)* 1012/L Less than ±5%

(20-70) g/L Less than ±2g/L

HGB Less than ±3%
(71-240) g/L

(20-100)* 109/L Less than ±10* 109/L

PLT
(101-999)* 109/L Less than ±10%
Composition
Instrument structure
•Front view

4
3
2

1 -Screen 2- Printer 3 -Probe 4 -Aspiration switch

5 -Bio hazardous symbol
• Back view 1-External cnnection
port （ PRINTER ）
2- Power switch
3-power socket
9 4-Dilution connection
1
port
10
5-Cleaning liquid
connection port
6- Waste connection
port
2
7-Cleaning liquid
4 sensor socket
3
8 8-dilution sensor
5 socket
7 9- Communication
6 port （ RS-232 ）
10-barcode reader
port （ BAR CODE ）
Front view with door open
1 1-Elevation motor
2-Probe
3-Swab
4-WBC cover
4 3 5-RBC cover

5
View of left door open 1-Pressure bottle
2-negative pressure Pump
3-positive pressure pump

2
3
1--- valve 8 2---volumetric unit
3---vacuum chamber 4---valve 14
5---valve 13 6---valve 9
7---valve 12 8---valve 11
9---valve 10 10--- valve 2
11---2.5ml and 50ul motor
12---10ml motor
13---2.5ml syringe 14---50ul syringe
15---10ml syringe 16---valve 4
17---valve 3 18---valve 1
19---valve 6 20---valve 5
21---valve 15 22---valve 16
23---valve 17 24---valve 7
25--valve 18
BCC-3000B electrical board

• CPU Main control board

• Drive board
• AD board
• Quantitative board
• Print drive board
Http://www.dirui.com.cn
CPU Main Control board
• It is composed of several parts: Computer system, Display module
circuit, A/D module circuit, I/O module circuit.
Drive board
• Accroding to the order of CPU board, Power drive board carry
out the operation to syringe unit, probe motor ,pump and valves.
AD board
• AD board mainly transforms the analog signal to the one meeting A/D requirement. It
include : WBC and RBC+PLT magnification unit, HGB magnification unit , temperature
measuring unit, vacuum measuring unit . Power monitor unit ( change all output value
into 0 to 5v voltage available for A/D conversion) , and plus power supply unit, pore
voltage disposal unit, electron simulation switch etc.
Quantitative board
• Volume meter board guarantees the precision of WBC and
RBC+RLT by burette and measure photocoupler calculating liquid
volume
 Printer drive board
• There is a SMC on the printer drive plate, who communicate with
CPU by serial port and main control plate, print out the character
and wave shape according to the CPU order, and send the status of
recorder to main CPU for display.
 Whole Blood13uL

Diluent 3.5 mL

Ratio 1 ： 269 Take 15.6ul mixed sa

mple to RBC bath

Lyse 0.5 mL Diluent 2.6 mL

Ratio 1 ： 308 For WBC and Ratio 1 ： 44872 For RBC a

HGB test nd PLT test
Tubing diagram
Maintenance
 System status
The information and the normal range of system status, such as ambient
temperature, counter HGB zero voltage, HGB background voltage, power supply
voltage, vacuum chamber pressure and the pressure chamber pressure, are
displayed on the main interface.
~ system status ~

Item Detection value Range

ambient temperature 26.0 15.0-30.0
HGB zero voltage 0.00 0.00-0.20
HGB background voltage 1.8 1.40-2.50
DC +12V 　 11.71
11.04-12.96
DC -12V 　 　 11.84
-11.04--12.96
106V(V) 108 100-112
56 V(V) 56.40 53.00-59.00
Vacuum chamber vacuum HP 201 175-205
Test
pressure chamber pressure HP 67 40-70
Exit
 Valve check
Valve switch can be checked whether it is normal in the valve interface. System
liquid lines will not work normally if the valve fails.
~Valve Check~

Valve Valve Valve Valve Valve

V1 OFF V5 OFF V9 OFF V13 OFF V17 OFF

V2 OFF V6 OFF V10 OFF V14 OFF V18 OFF

V3 OFF V7 OFF V11 OFF V15 OFF

V4 OFF V8 OFF V12 OFF V16 OFF

Confirm Exit
Description and use of menu of instrument system check and diagnosis
Total 19 tests, such as counter hardware, machinery parts and liquid line
components, are tested by system, and it is shown that whether the component is in
normal state or whether the time of the test is in the range of time.
System testing is fluid path for a total of 19 tests showed or to the time required for
this operation. Check whether 2.5ml and 50ul, 10ml, deflection and up-down motors
run normally in all directions.
System Testing ~

No. Item Result No. Item Result

1 WBC A/D status 11 Sampling motor
2 RBC A/D status 12 10ml motor
3 PLT A/D status 13 Deflexion motor
4 WBC A/D intermitted 14 Up-down motor
5 RBC A/D intermitted 15 Printing
6 PLT A/D intermitted 16 Vacuum of vacuum chamber
7 WBC path timing Pressure of vacuum chamber
8 RBC path timing 17 Pressure of pressure chamber
9 WBC ventage voltage 18 Filter of vacuum chamber
10 RBC ventage voltage 19

Confirm Up Down Exit

1 ． Press 【 Down 】 to move icon to any item, press 【 Confirm 】 key,
system starts to test item chip.

2. If the result is normal, “Normal”(Y) will be displayed in the corresponding

result column of the "Test Item" .

3 ． If test results are abnormal, failure (N) will be displayed in the

corresponding result column of the "Test Item", and refer to malfunction at
this time or measure the test points of relative valtage.

4. Press 【 Exit 】 to exit the system monitoring interface.

Regular maintenance

Daily 1, if instrument is not shut down for 24-hour, conducting

"counting cell cleaning" daily is a must (not less than 40 minutes
immersing is recommended) or press [Rinse] button to carry out
the rinsing operation

2 、 QC

Every 3 if instrument is not shut down for 24-hour, conduct "Immerse

days probe top" every 3 days is a must.

Weekly If instrument is shut down normally every day, conduct "Immerse

probe top" weekly

Monthly Calibrate sampling probe with sampling probe height adjustor

 Maintenance when needed

● If the counting cell is contaminated, implement “Counting cell cleansing "

operation;
● When the counter is not used for more than two weeks, implement " Exhaust
liquid line" operation in "System maintenance" menu to complete the counter liquid
line exhausting and cleaning, and wipe counter and place it in a clean site;
Press 【 Eliminate block 】 key manually or execute “Burn gem hole” and “Back-
flush gem hole” operation.
● Reliable analysis result can be obtained when the counter works in a normal
circumstance. So, counter working status should be confirmed by implementing
“System check” operation.
 Method
10 ways are available in the system maintenance
interface
System maintenance

１ . Diluent prime 6. Probe cleanser

２ . Cleanser prime 7. Clean baths

３ . Lyse prime 8. Wipe block cleaning

４ . Zap apertures 9. Empty tubing

5. Flush apertures 10. Empty baths

Return
Troubleshooting
 Malfunction and resolvent
If analyzer detects some unnormal situations, the relevant malfunction
reminding will show on the screen. The following is the guidelines for
resolving problem.

WBC blank value high

1 ． Check if there are earthing wire in instrument inner
2 ． Interwiew the histogram on the screen.
3 ． Dip in the counting cell with probe washing solution
4 ． Open the front door, avoid electric circuit interfere
5 ． Observe if there are bubble in 10ml syringe.
6 ． Detach,wash front and rear counting cell, aperture. Replace the aperture’s washer
7 ． Stop adding hemolysin.
8 ． Observe the tube under counting cell,if it is dirty,clean it.
9 ． Valve dirty, close loosely, open hardly
10 ． Replace counting cell
11 ． Simulation plate break, fix it.
 PLT Blank value high

1 ． If blank value are stable, change reagent.

2 ． Check if there are earthing wire
3 ． Interwiew the histogram
4 ． Dip in the counting cell with probe washing solution
5 ． Detach and wash front cell, rear cell, aperture. Replace
the aperture’s washer
6 ． V11 Valve dirty, close loosely, open hardly
7 ． Replace cell.
8. Aperture
9 ． AD board
 WBC Blood sample value high

1 ． Check if the blank value is normal

2 ． Check if demarcate coefficient is correct.
3 ． Check if there are hemolysin, and its volume.
Check 2.5ml syringe and valve
4 ． Valve is dirty, valve slice break.
5 ． Simulation plate
6 ． HGB adjust alarm
7 ． Review the voltage of original value
8 ． Dip in and wash WBC cell .
 RBC.RLT Blood Sample Value Low
1 ． Cell does not completely discharge, To open /close valve
is not smooth
2 ． To add and dilute sample by sampling probe is normal or
not.
3 ． Simulation plate

WBC Blood Sample Value Low

1 ． Cell does not completely discharge, To open /close
valve
is not smooth . ( RBC value is also lower at one time) 2 ．
Sampling probe is block, sampling volume is not enough.
(RBC value is also lower at one time)
3 ． The distance between sampling probe and 10ml syringe
leak out. (RBC value is also lower at one time)
Blood sample counting: WBC value is normal; RBC
value a few; PLT value zero or several decades or
normal

1 ． Diluent and washing solution connect reversely

2 ． Vacuum tightness is low.
3 ． Waste tubing is pressed,discharging liquid is not easy.
4 ． Vacuum filter is blocked
5 ． The malfunction of vacuum pump
6 ． Valve is not close tightly.
7 ． Vacuum tank and its relevant pipeline are leaking
8 ． The pipeline of vacuum sensor on simulation plate has

crystal .
Fluctuating motor/ rotatory motor malfunction

1 ． Temperature is too low

2 ． Motor is too large
3 ． Upper photocoupler is wrong
4 ． Lower turning photocoupler is wrong
5 ． Motor is wrong
6 ． Power drive plate is wrong

At turning on, black screen happens.

1 ． If pilot lamp of power is on

2 ． If it is initializing.
3 ． Electron plate is wrong .
4 ． Main control plate is wrong
5 ． Power supply plate is wrong .