Statistical Treatment

To interpret the data effectively, the researchers will employ the following statistical data
treatment, the T-test and ANOVA.
Further data analysis will be carried out using T test for statistical examination of two (2)
population means. A two-sample T test will be examined whether the two (2) samples were
different and was commonly used when the variances of two (2) normal distributions were
unknown and when an experiment uses a small sample size. The researchers considered that the
T test is the most useful method for comparing the means of two (2) samples (or treatments),
even if they had different numbers of replicates. In simple terms, the T test compares the actual
difference between two (2) means in relation to the variation in the data (expressed as the
standard deviation of the difference between the means).
T test will be used to compare two (2) different set of values. It was generally performed
on a small set of data. T test was generally applied to normal distribution which has a small set of
values. This test compared the mean of two (2) samples.
The zone of inhibition implied the effect of the treatment to a specific microbial agent. It
will be used to test antimicrobial properties of a certain treatment, it will also be used clinically
to measure the antibiotic resistance and industrially to test the ability of solids and textiles to
inhibit microbial growth, and it will be also an easy way to measure and compare levels of
inhibitory activity.
Kirby-Bauer technique or disc diffusion test will be used to determine if an organism was
susceptible or resistant to a selection of antimicrobial agents. When it was performed in a
specific manner, it also could be used to determine how susceptible an organism was to a
specific antimicrobial agent.
T test will use means and standard deviations of two (2) samples to make a comparison in
determining the statistical significance of the zone of inhibition of C.longa from its gathered
extract against Multi-Resistant Staphylococcus aureus and Pseudomonas aeroginosa.

Analysis of Variance (ANOVA) is a statistical method that will be used to test

differences between two or more means. It is a collection of statistical models and their
associated procedures (such as “variation” among and between groups) used to analyze the
differences among group means. It was developed by a statistician, and a biologist Ronald
Fisher.
In ANOVA, the observed variance in a particular variable was partitioned into
components attributable to different sources of variation. In its simplest form, ANOVA provided
a statistical test of whether or not the means of several groups are equal, and therefore
generalizes the T test to more than two (2) groups. ANOVA was useful for comparing (testing)
three (3) or more means (groups or variables) for statistical significance.