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Cheese: Processing and Sensory Properties

JM Kongo, INOVA, Instituto de Inovação Tecnológica dos Açores, Ponta Delgada, Açores, Portugal
FX Malcata, LEPABE/Department of Chemical Engineering, University of Porto, Porto, Portugal
ã 2016 Elsevier Ltd. All rights reserved.

Introduction added as a preservative, flavor enhancer, and syneresis promoter,


reducing also the aw of the curd. Finally, the pressed curds are put
Cheesemaking implies the elimination of whey (the watery por- to the ripening process so that the required biochemical reac-
tion of milk) after coagulation of milk, thus resulting in the tions that lead to specific flavors will occur.
concentration and preservation of the most important milk Fresh, soft, or hard cheeses are made essentially by the same
nutrients – protein, fat, and minerals. For many cheeses, namely, process with slight differences in one or more of the steps
those undergoing the process of ripening, the optimization of described (see Figure 1). Thus, while fresh cheeses are ready
coagulation and whey elimination (syneresis) are obtained by for use as soon as the manufacturing process is complete, hard
first adding a lactic acid bacteria (LAB) starter culture to milk. At cheeses are allowed to mature in temperature- and humidity-
this initial phase, the starter culture is expected to cause a slight controlled rooms from 1 to 24 or more months depending on
and rapid acidification of milk through the production of lactic the type of cheese.
acid, with the consequent decrease in milk pH. Next, rennet (an From coagulation, cutting, and pressing, the pH of the
acidic enzyme) is added to coagulate the milk, and this, with the curds will usually change markedly (see Figure 2). Later, dur-
contribution of the subsequent cheesemaking steps, leads to ing ripening, the pH will usually keep dropping in most cheese
formation of the initial texture and the almost insipid flavor of varieties as shown in Table 1, and the pH of the final cheese
the cheese. Ripening, a slow process that may last from 1 to 24 will usually vary between 4.8 and 5.4 as a result of the contin-
months depending on the cheese variety, is the final step that uous acidification by the starter culture, which also contributes
imparts the final and most significant changes of cheese flavor. to the release of volatile components in the ripening curd.
Thus, the final sensorial features of a cheese are more or less a
consequence of the combined actions of all steps involved in its
processing. Consequently, understanding and controlling the Cheese Texture
effect of each of the processing steps during cheesemaking is
crucial toward directing the process to deliver the final product Texture can be defined as the sensory manifestation of struc-
in mind. Also, due to the key role the sensorial factors play in the ture of the food and the manner in which this structure reacts
cheese consumer’s choice, attempts to understand and control to applied forces. Texture affects the immediate perception of a
the dynamics of the development of texture and flavor have been consumer to the quality of a product, through vision, kinesthe-
an important part of the activity in the field of food science. sia, and hearing, making it a key flavor indicator. The final

Manufacturing Ripening
Milk Fresh Curd Cheese
Cheese Processing and Starter Cultures Selection of milk 1 to 24 months
Acidification
The basic principles involved in cheese manufacture have
remained the same for over 1000 years. Basically, milk must be Figure 1 Essential steps involved in most cheese processing.
first heat treated to kill the harmful bacteria that it may harbor.
Starter cultures, usually LAB, are then added to the milk and their
growth ‘ripens’ the milk and later helps to develop the desired
flavors and aromas in the cheese. For raw milk cheeses, in case a 6.6
starter culture is added, it can be of a pure or mixed undefined
(whey from previous day) type. The starter culture produces 6.4
lactic acid, a natural preservative that, besides acting on cheese 6.2
taste, will cause the pH of milk to drop, thus creating optimal
6.0
conditions for the activity of rennet that is usually added after-
pH

ward. This will cause the coagulation of the main protein present 5.8
in milk - casein - by lowering its solubility, leading to its precip- 5.6
itation and formation of a gel that will separate into curds and
5.4
whey. LAB may later have a different number of metabolic
activities, namely metabolizing citric acid, acting on the break- 5.2
down of the protein (in conjunction with the rennet and
Milk Cutting Pressing day 1
enzymes from milk), as well as on the breakdown of the diglyc-
erides formed from the milk triglycerides, and in some cheeses, Cheese processing step
on the breakdown of hippuric acid to benzoic acid. The formed Figure 2 Evolution of pH (average þ standard deviation) in an
curds are then heated according to the type of cheese, and salt is experimental cheese.

748 Encyclopedia of Food and Health http://dx.doi.org/10.1016/B978-0-12-384947-2.00136-7


Cheese: Processing and Sensory Properties 749

Table 1 Typical pH versus time profiles for several cheese varieties (time is in minutes unless otherwise noted)

Swiss-type Gouda Cheddar Feta Cottage

Operations Time pH Time pH Time pH Time pH Time pH

Add starter 0 6.60 0 6.60 0 6.60 0 6.60 0 6.60


Add rennet 15 6.60 35 6.55 30 6.55 75 6.50 60 6.50
Cut 45 6.55 70 6.50 75 6.50 115 6.4 300 4.80
Drain or dip into forms 150 6.35 100 6.45 195 6.3 130 NA 360 5.0
Milling NA NA NA NA 315 5.45 NA NA NA NA
Pressing 165 6.35 130 390 5.40 NA NA NA NA
Demolding 16 h 5.30 8h 5.40 10 h 5.20 24 h 4.6 NA NA
Minimum pH 1 week 5.20 1 week 5.20 1 week 5.10 1 week 4.4 NA NA
Retail 6 months 5.6 6 months 5.6 4 months 5.3 6 weeks 4.4 2–14 days 5.2

texture of a cheese is developed throughout ripening, a slow Table 2 Definitions of textural properties attributed to cheeses and
process involving the concerted series of biochemical, other dairy products
microbiological, and chemical reactions, which are predeter-
Textural
mined by the manufacturing processes. Generally, a cheese
properties Definition
may be classified as having a typical soft, semisoft (medium),
or hard texture, and the said classification is related to the Adhesiveness Work necessary to overcome the forces between
cheese water and fat contents, to which many factors will dissimilar materials
contribute. Chewiness Energy required for masticating a solid food material
As previously stated, processing conditions – temperature until it is ready for swallowing
of coagulation, cutting and cooking the curds, pressing, salting, Cohesiveness The strength of the internal bonds making up the body
and ripening – will affect directly the cheese texture or hard- of the product or degree to which the sample
deforms before rupture
ness. The pH at drain determines the retention of rennet
Gumminess Energy required to disintegrate a semisolid food
(which participates in hydrolysis of as1-casein) and the amount
material to a state ready for swallowing
of plasmin (a native milk proteinase that is responsible for Hardness Force necessary to attain a given deformation
much of casein breakdown in the curd), while the salt-to- Resilience How well a product fights to regain its original position
moisture ratio affects the amount of intact casein. Finally, Springiness Degree or rate at which the sample returns to its
the changes in body and texture that transform the rubbery, original shape/size after partial compression
elastic mass of curd to a cheese with a firm close texture between tongue and palate
are essentially the results of the three primary biochemical
processes – glycolysis, lipolysis, and proteolysis – occurring
mainly during ripening. With aging, the cheese texture will syneresis and the use of specific types of vegetable curds
also change as a result of the evaporation of available water (such as cardosin) or starter cultures (viz., specific fungi of the
on the surface, leading to a ‘drier,’ harder, and less deformable group Penicillium), which are highly proteolytic and thus will
cheese. Finally, the storage temperature (or ripening tempera- contribute to loosening the matrix formed by casein to peptides
ture) impacts the rate of proteolysis, namely, proteolysis of and amino acids, involving high fat content and water.
as1-casein hydrolysis. In conclusion, the texture of a cheese is a reflection of its
In general, cheese texture will change markedly in the first microstructure, formed essentially by the casein matrix entrap-
1–2 weeks of ripening as the hydrolysis of a small fraction of ping fat globules and bounded to water, forming a structure
as1-casein by the rennet to the peptide as1-I results in a general that is affected by the biochemical activities occurring during
weakening of the casein network. The following and usually ripening, which change the amount, type, and physicochemi-
relatively slow (occurs over a period of months) change in cal relation of the said components.
texture is often associated with the rate of proteolysis, which A cheese may have several important textural properties as
in turn is controlled largely by the proportion of residual listed in Table 2.
rennet, plasmin, microflora peptidases, salt-to-moisture ratio,
and storage temperature.
Cheese composition also affects the final texture, as both Flavor in Cheese
dissolved calcium in the cheese serum and calcium bound to
the protein network have been shown to affect the rate of While the perception of a cheese texture also contributes to its
proteolysis, and cheeses having a higher fat content are less general flavor, texture may be seen as a simpler contact by
firm and more elastic. means of the sense of touch, while flavor usually requires a
Sodium chloride affects both the matrix and the serum more profound use of our senses of vision, taste, and smell
phases of the cheese, which, in turn, affects the overall texture. with the food product.
It is known that brine-immersed cheeses show dramatic As previously stated, the initial fresh curds from any cheese
changes in texture during the early stages of aging. Many soft type taste more or less the same. It is only during ripening that
cheeses are obtained by a combination of a low degree of the biochemical processes occurring by the action of LAB and
750 Cheese: Processing and Sensory Properties

milk native enzymes present in the curd will cause the main Glycolysis
contribution to cheese flavor development. Those primary
The fermentation of lactose is one the first LAB activities that
changes are followed and overlapped by a host of secondary
contribute to flavor in cheese. The general simplified pathway
catabolic changes, including deamination, decarboxylation,
of lactose metabolism (glycolysis) by LAB (starter and
and desulfurylation of amino acids and b-oxidation and ester-
nonstarter LAB (NSLAB)) in cheese is shown in Figure 3.
ification of fatty acids. While the primary reactions are mainly
Lactose can be metabolized to lactate and then to acetate
responsible for the basic textural changes of the curd and for
and diacetyl by strains of Lactococcus lactis diacetylactis and
the basic flavor of cheese, the secondary transformations are
Leuconostoc spp. and CO2, which is responsible for the
responsible for the finer aspects of cheese flavor and also
characteristic eyes of many cheeses, and to citrate that contrib-
modify the texture. A number of metabolites, important con-
ute to flavor.
tributors to a cheese’s flavor, are formed from the three main
In the case of many traditional cheeses, a mixed culture of
milk components during ripening, as shown in Tables 3 and 4.
unknown composition is used in the so-called back slop
It can thus be stated that a cheese flavor is composed of a
method.
complex large number of dissolved volatiles of low-molecular-
weight compounds and ions, which act synergistically in the
perception of flavor, and their sensory contribution may not be Lipolysis
directly dependent on their comparative concentration. If in
excess, any of these metabolic products may also result in an Lipolysis in cheese is important as a process leading to the
off-flavor appearance in cheeses. formation of many flavor metabolites due to the activity of
Many of the enzymes involved in the said processes may be
dependent on cooperation between strains, making the meta-
bolic processes by LAB important in flavor formation. Lactose

Starter culture
Biochemical Reactions and Cheese Flavor Development

Cheese flavor is a direct result of three biochemical reactions


L- Lactate
generally known as glycolysis, lipolysis, and proteolysis.

Table 3 Flavor compounds formed from main milk components


NSLAB
during ripening

Casein Milk fat Lactose and citrate


DL- lactate
Peptides Fatty acids Lactate
Amino acids Keto acids Pyruvate
Acetic acid Methyl ketones CO2
Ammonia Lactones Diacetyl
NSLAB
Pyruvate Acetoin
Aldehydes 2,3-Butanediol
Alcohols Acetaldehyde Acetate
Carboxylic acid Acetic acid
Sulfur compounds Ethanol
Figure 3 Simplified pathway of lactose metabolism in LAB.

Table 4 Some key flavor components in known variety of cheeses

Cheese variety

Metabolism of Gouda Cheddar Camembert Swiss-type

Peptides/amino acids 3-Methylbutanal 3-Methylbutanal 3-Methylbutyrate Methional


Methanethiol Isovaleric acid Methanethiol 3-Methylbutanal
Dimethyl sulfide Methanethiol Benzaldehyde Skatole
Methylpropanol
Sugar Diacetyl Propanoic acid 2,3-Butanedione Propanoic acid
Diacetyl Diacetyl
Fat Butyric acid Butyric acid Butyric acid Ethyl butyrate
Butanone Acetic acid 1-Octen-3-ol Ethyl hexanoate
Hexanal Butanone 2-Undecalactone Ethyl-3-methylbutanoate

Source: Smit G, Smit BA, and Engels WJM (2005). Flavour formation by lactic acid bacteria and biochemical flavour profiling of cheeses products. FEMS Microbiology Reviews 29:
591–610.
Cheese: Processing and Sensory Properties 751

Triglycerides

Lipases

Fatty acids

Secondary alcohols Free fatty acids Lactones Acids Alcohols

Flavor compounds

Figure 4 A simplified pathway of milk triglyceride and fatty acid flavor compound formation during cheese ripening.

such enzymes as lipases and esterases. The hydrolysis of tri- by the microflora. Products such as keto acids, aldehydes or
glycerides, which constitutes more than 98% of cheese fat, is carboxylic acids, ketones, lactones, esters, alcohols, aldehydes,
the main biochemical transformation of fat occurring during pyrazines, sulfurous compounds, carbonyl compounds, FFAs,
ripening and causes the production of short-chain free fatty free amino acids, and salts have been detected and reported to
acids (FFAs) that contribute to the aroma of cheese, depending contribute to cheese aroma. In general, different nitrogen
on the amount of the aqueous phase and the pH of a cheese. catabolisms will result from different starter cultures, and
LAB play an important role in this process that leads to the such products as 3-methylbutanal, an important volatile com-
formation of flavor. The presence of specific FFA can give the pound formed during the ripening of Parmesan cheese, or
perception of flavor such as rancid, sharp, goaty, soapy, and methyl alcohols and methyl aldehydes derived from the
coconut-like. FFA can be further hydrolyzed to methyl ketones, branched-chain amino acids, leucine, isoleucine, and valine,
which are responsible for the characteristic aroma of blue may be formed. A general overview of proteolysis and the
cheeses. Lactones have also been identified as possessing pathway of formation of methylbutanal are shown in Figures 5
strong aroma that may be important in overall cheese flavor. and 6.
A simplified pathway of milk fat metabolism is shown in As consequence of the dynamic of activity of LAB through-
Figure 4. out ripening, cheese taste and texture will obviously change
with aging, as more biochemical reactions occur and because
moisture and salt content and pH also change throughout
Proteolysis ripening.
Proteolysis is probably the most important biochemical event
in cheese ripening accounting for the development of a num-
ber of organoleptic features, encompassing both flavor and Cheese Microflora and Flavor Development
texture.
Thus, early-stage hydrolysis (primary proteolysis), that Fermentation with LAB is a cheap and effective food preserva-
leads to the formation of large specific peptides, and the tion method that can be applied even in more rural/remote
later-stage proteolysis (secondary proteolysis) will occur places and leads to improvement in texture, flavor, and nutri-
when the said peptides are digested into smaller ones and tional value of many food products. LAB have a long and safe
even free amino acids by enzymes from starter or nonstarter history of application and consumption, namely, in cheese
microorganisms, leading to changes in texture and taste of the processing, thus, being generally recognized as safe.
cheese. Generally, the rate of breakdown of as1-casein is greater Cheesemaking is based on the application of LAB in the
at lower storage pH than the rate of breakdown of b-casein, form of defined or undefined starter cultures that are expected
thus, changes in pH during storage affect the rate of proteolysis to cause a rapid acidification of milk through the production of
and consequently texture. lactic acid, with the consequent decrease in pH, thus, affecting
Proteolysis in cheese is defined as changes in b-, g-, and a number of aspects of the cheese manufacturing process and
s-casein peptides and other minor proteins that lead to the ultimately cheese composition and quality.
formation of large water-insoluble peptides and smaller Traditionally, mixed or undefined strain starter cultures
water-soluble peptides from the action of rennet (chymosin), composed of a number of strains of Lactococcus spp., Lactoba-
milk native proteases, and peptidase enzymes from starter and cillus, and even Enterococcus spp. were used.
NSLAB. The hydrolysis of casein to high-molecular-weight The earliest productions of cheeses were based on
peptides is thought to be primarily the result of chymosin spontaneous fermentation, resulting from the development
and plasmin, while the subsequent hydrolysis of high- of the microflora naturally present in the raw milk and its
molecular-weight peptides is primarily the result of proteolytic environment. The quality of the end product was a reflex of
enzymes from LAB. In general, many proteolytic compounds the microbial load and spectrum of the raw material. Sponta-
contribute to the typical aroma of a cheese. They are a result of neous fermentation was later optimized through backslop-
different reactions including deamination, transamination, ping, that is, inoculation of the raw material with a small
decarboxylation, and cleavage of the amino acid side chain quantity of whey from a previously performed successful
752 Cheese: Processing and Sensory Properties

Cheese

Milk

LAB, Plasmin, and residual rennet


Lactose

αs1 and β-casein residues

LAB
Casein Flavor and
Proteins, Peptides, and free amino aroma
+ acid
formation
Free fatty acids
Rennet Lactones, and other components

Figure 5 General view of proteolysis during cheese processing.

proteolysis transamination descarboxylation


Casein Leucine caproic acid 3-methyl butanal

Figure 6 3-Methylbutanal formation by LAB during ripening of cheese.

fermentation, and the resulting product characteristics Undesirable flavors, namely, bitterness, may develop in
depended on the best-adapted strains dominance. Today, cheeses, often as consequence of combined excessive activity
backslopping is still used to produce many artisanal raw milk of rennet and mesophilic starter cultures. Bitterness is consid-
cheeses, namely, those bearing the PDO (Protected Designa- ered a result of excessive accumulation of bitter peptides (usu-
tion of Origin) status, which are considered to be an important ally of 2–23-amino-acid residues), predominantly containing
source of LAB genetic diversity. The starter culture applied in hydrophobic amino acids. Excessive use of certain types of
this, so-called natural fermentation, is usually a poorly known rennet and starter cultures with high proteolytic activity can
microflora mix that, although having a predominance of LAB, lead to formation of bitterness and it has been shown that this
may also contain non-LAB microorganisms, and its microbial defect often occurs in cheeses made without NaCl and in low-
diversity and load are usually variable over time. fat cheeses. Also, it seems that fast acid producers, heat-tolerant
Moreover, traditional cheeses also obtain their flavor inten- strains grown under uncontrolled pH conditions, are more
sity from the NSLAB, which are not part of the normal starter prone to producing high levels of bitter components. Off-
flora but develop in the product, particularly during matura- flavors such as rancidity may develop due to excessive or
tion, as a secondary flora. unbalanced lipolysis caused by lipases/esterases from starter
In the high-output cheese industry, defined cultures are or NSLAB, from enzymes produced by psychrotrophic bacteria,
rather used, optimizing the process, namely, the lactic acid or from indigenous milk lipoprotein lipase. Finally, late gas
formation. Bacteria existing in the processing environment – blowing and other off-flavors in certain hard cheeses result
the so-called nonstarter lactic acid bacteria – often contaminate from the metabolism of lactate (or glucose) by Clostridium sp.
the cheeses, thus, having also a role in flavor development. For to butyric acid and H2.
specific varieties of cheese, such as ‘blue cheeses,’ a starter
culture made up of specific molds such as the Penicillium spp.
is also used. Due to a lower diversity of the microflora in
industrial cheeses, they are commonly recognized as develop- Texture and Flavor Analysis
ing weaker flavors.
The starter culture in cheesemaking is thus very important While texture (mouthfeel) of a food product may be subjective,
for two obvious reasons of producing lactic acid via metabo- it plays a key role in consumer acceptance and market value of
lism of lactose: lowering the pH of the curd (recall that at lower a food product, namely, cheeses. The sensory measurements of
pH values, undesirable bacteria have more difficulties to grow a cheese may be taken by consumers (say, in shopping malls,
and therefore the pH history during processing of the cheese is supermarkets, or any other area having a large number of
a good indicator of the actual product safety) and producing consumers) or by a trained panel. The purpose of a consumer
the distinctive flavors and textures via metabolism of fat, taste test is usually for a general evaluation (acceptance/
lactose, and protein. rejection) of a dairy product, while the trained panels are
Cheese: Processing and Sensory Properties 753

trained to detect a specific food characteristic (bitterness, sand- spectroscopy, while mechanical properties such as firmness
iness, and crumbliness) with a high degree of reproducibility. and hardness (texture profile analysis (TPA)) may be evaluated
However, attempts have been made to standardize mea- by mechanical methods using a testing machine such as the
surements of texture and flavor. In fact, food texture character- Instron model (Figure 7), a penetrometer, or a rheometer.
ization is rooted on human sensory evaluation; however, many Figures 8 and 9 are results of a study of texture characterization
instrumental methods have been developed to analyze some of samples of São Jorge cheeses at different ripening periods.
physical and chemical properties in finished products, allow- Due to good separation efficiency and versatility, gas chro-
ing for corrections and control of the manufacturing and stor- matography methods have found increasing acceptance and
age processes in order to consistently deliver the expected final application in food science and in technology for separation
product. and identification of a large variety of compounds. These tech-
The evolution of a cheese microstructure can be followed by niques are thus helping to elucidate and understand the mech-
microscopy techniques, generally known by such acronyms as anisms and chemistry of flavor formation in cheeses.
TEM, SEM, and CLSM, imaging techniques, and infrared A cheese map developed for cheddar cheeses is shown in
Figure 10. Different cheddar cheeses from around the world
can be placed in the map due to their specific flavors.
In conclusion, cheese flavor and texture are complex and
slow processes resulting from the extent of the biochemical
pathways of glycolysis, lipolysis, and proteolysis, which are

90 days 120 days 210 days


6.0

Stress (kPa) 5.0

4.0

3.0

2.0

1.0

0.0
0.0 0.2 0.4 0.6 0.8
Strain
Figure 9 Stress–strain curves of São Jorge cheese with 90, 120, and
210 days of ripening.

Figure 7 Texture testing machine Instron model 4501, series H3279.

1.2

1.0
0.662 a
0.8 0.690 a
Hardness (kg)

0.556 b 90 days

0.6 120 days


210 days
0.4

0.2

0.0
Ripening time (days)
Figure 8 Hardness of São Jorge cheese (average  standard deviation,
n ¼ 154) by 90, 120, and 210 days of ripening. Different superscript
letters represent mean values that are statistically different for p < 0.05. Figure 10 Cheddar cheese flavor map.
754 Cheese: Processing and Sensory Properties

affected by the cheesemaking specificities and the microflora Lin H, Boylston TD, Luedecke LO, and Shultz TD (1999) Conjugated linoleic acid
(starter and NSLAB) present in the curd and by the native content of cheddar-type cheeses as affected by processing. Journal of Food Science
64: 874–878.
enzymes of milk and rennet. Studies and attempts to control
McSweeney PLH (2007) Cheese problems solved. Cambridge: CRC Press.
cheese flavor are important activities in food science due to the Moatsou G, Massouras T, Kandarakis I, and Anifantakis E (2002) Evolution of
key role flavor and texture play in the consumer’s choice of a proteolysis during the ripening of traditional Feta cheese. Lait 82: 601–611.
cheese. Mullan, W. M. A. (2005). Role of cheese starters (online). Available from: http://www.
dairyscience.info/index.php/cheese-starters/225-role-of-starters.html (accessed 20
May 2014).
Nollet LML and Toldra F (2010) Handbook of dairy foods analysis. Boca Raton, FL: CRC
See also: Cheese: Chemistry and Microbiology; Cheese: Composition Press.
and Health Effects; Cheese: Types of Cheese – Medium; Cheese: Types Sigh TK, Drake MA, and Caldwallader KK (2003) Flavor of Cheddar cheese: a chemical
of Cheeses – Hard; Cheese: Types of Cheeses – Soft; Fermented and sensory perspective. Comprehensive Reviews in Food and Science and Food
Foods: Fermented Milks; Lactic Acid Bacteria. Safety 2: 139–161.

Further Reading
Adams MR and Moss MO (1995) Food microbiology. Guildorf: Royal Society of Relevant Websites
Chemistry, University of Surrey.
Araujo VS, Pagliares VA, Queiroz MLP, and Freitas-Almeida AC (2002) Occurrence of http://www.cheese.com/.
Staphylococcus and enteropathogens in soft cheese commercialized in the city of http://www.cheesesociety.org/ – American Cheese Society.
Rio de Janeiro, Brazil. Journal of Applied Microbiology 92: 1172–1177. http://dairyscience.info/cheese-starters/49-cheese-starters.html – Dairy Science and
Eck A (1987) O Queijo (Le Fromage). Portugal: Europa America Publisher. Food Technology.
Garabal JI (2007) Biodiversity and survival of autochthonous fermented products. http://www.ehow.com/how-does_4571415_how-cottage-cheese-made.html – eHow.
International Microbiology 10: 1–3. http://www.foodprocessing.com/articles/2008/047/ – Food Processing.
Kosikowski FV and Mistry VV (1997) Cheese and fermented milk foods, 3rd ed. http://www.thedairysite.com/articles/2875/european-cheese-market – The Dairy Site.
Brooktondale, NY: F.V. Kosikowski and Associates. https://www.uoguelph.ca/foodscience/ – University of Guelph.

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