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ABSTRACT: Chicken meat powder and chicken meat at five different levels (10, 15, 20, 25, and 30%)
was added to white and whole wheat bread for protein enrichment and nutritional, sensory, and quality
characteristics of these enriched bread samples were determined. The protein content of the bread
samples with the addition of chicken meat and chicken meat powder were increased from 7.60% to
18.44% and 18.70% for white bread, and from 8.85% to 14.23% and 16.49% for whole wheat bread,
respectively. The total colour difference of bread crumbs was increasing with the increasing level of
chicken meat powder and chicken meat (p<0.01). For white wheat bread, enrichment didn’t present
any significant effect on crumb firmness, while for whole wheat bread the firmness was increasing with
the level of enrichment (p<0.01). Five different sensory attributes like crumb colour, crust colour,
crumb firmness, crust firmness, and overall acceptability, were assessed to determine the differences
among the enriched bread samples. These attributes of breads were significantly affected by the
addition of chicken meat powder and chicken meat.
Key words: white bread, whole wheat bread, chicken meat, chicken meat powder, protein enrichment
INTRODUCTION
Wheat and wheat products are the major (Pyler, 1973), wheat flour should be consi-
staple foodstuffs in the human diet. They dered as a good source for enrichment
are source of calories and contribute and fortification as it is regularly consumed
significant quantities of other nutrients by large populations (WHO et al., 2009).
such as vitamins, minerals, and dietary To define the terms; fortification is des-
fibre (Sidhu et al., 1999). Bread and other cribed as increasing the content of essen-
wheat products are one of the most con- tial micronutrients, i.e. vitamins and mine-
sumed foods in the daily diet of all people rals, including trace elements, in a food so
around the world, although the level of as to improve the nutritional quality of the
consumption varies from region to region food supply and provide a public health
(Cauvain, 1995). According to the re- benefit with minimal risk to health, while
search of the Turkish Society of Cardio- enrichment is the addition of microntrients
logy (Istanbul, Turkey), 37% of the daily to a food irrespective of whether the nu-
energy intake of the Turkish people is ful- trients were originally in the food before
filled by cereals and other related products processing or not. To deal with malnu-
while meat and poultry products fulfill only trition problems, some strategies were
6% (Aslan et al., 2006). Since fortification developed at the International Conference
of white flour and white bread with thia- on Nutrition with a consensus by 159
mine and riboflavin is not a new subject countries worldwide about the enrichment
Cakmak, H. et al., Chicken meat added bread formulation for protein enrichment,
Food and Feed Research 40 (1), 33-41, 2013
of foods (Allen et al., 2006). Since wheat The aims of this research are to invest-
products are the main item of a daily diet, tigate possibilities of using chicken meat
enrichment techniques are mostly studied (chicken meat and chicken meat powder)
on this food. as an animal protein source in bread and
determine the nutritional, sensory, and
Various alternatives for enrichment of quality characteristics of those enriched
bread have been discussed in literature. breads. Therefore two types of flour, white
Oliete et al. (2008) are enriched breads wheat flour (type 650) and whole wheat
with nut pastes to determine the morpho- flour were used in bread making studies.
geometric and textural characteristics
along with the consumer acceptability of MATERIAL AND METHODS
the breads. Conforti and Cachaper (2009) MATERIALS
have studied the flaxseed meal enrich- The white wheat flour and whole wheat
ment of bread based on the richness of flour were obtained from Yuksel Tezcan
flaxseed with dietary fibre, protein, vi- Gida Sanayi ve Ticaret A.S. (Izmir, Tur-
tamins, and minerals. Carob fibre, inulin, key). The chicken meat powder and chi-
and pea fibre addition to bread with res- cken meat were supplied from Banvit A.S.
pect to bread quality and dough perfor- (Balikesir, Turkey) and Keskinoglu Tavuk-
mance was also studied (Wang et al. culuk A.S. (Manisa, Turkey), respectively.
2002). The average particle size of white flour
Dietary fibre enrichment has gained more and chicken meat powder was 205 μm
importance in the prevention of cancer, and 390 μm, respectively. Two types of
cardiovascular diseases, and reduction of baking improvers namely EMCE gluten
high cholesterol levels (Mesci et al., 2011). plusP and Top Bake Pitta (ABP Mühlen-
chemie Gida San. ve Tic. A.S., Izmir,
This health promoting effect may be
Turkey) with a rate of 0.4% (on flour ba-
caused by plenty of vitamins, minerals,
sis) were blended with 70% whole wheat
dietary fibre, phytochemicals, and phyto-
flour and 30% white flour to obtain whole
sterols found naturally in whole grains
wheat flour blend.
(Jonnalagadda et al., 2011).
METHODS
Vegetable proteins (Bildstein et al., 2008;
El Adawy, 1997; El Soukkary, 2001; Chemical and technological properties
Khalil, 2002; Seguchi and Abe, 2003; Se- of raw materials
guchi et al., 2001, Škrbic and Filipčev, The farinograph and extensograph ana-
2008) and animal proteins i.e. supplied lysis were conducted to examine the rheo-
from tench fish (Dursun et al., 2009) were logical properties of dough (ICC, 1992).
used for protein enrichment in breads. The moisture (ICC, 1976), and ash con-
The concept of the ideal protein provides tents of flour samples (ICC, 1990) were
that all amounts of essential amino acids determined according to ICC Standard
are balanced against each other and the Methods. The protein content of the flour
nonessential amino acids are according to samples were measured according to the
the nutritional needs of the organism. Ce- AACC method (AACC, 2000). The mois-
real proteins, on the other hand, are un- ture, ash, and protein contents of chicken
balanced in terms of essential amino acids meat powder and chicken meat were
and deficient in the essential amino acids analysed according to AOAC methods
lysine and threonine (Dhingra and Jood, (AOAC, 1991; AOAC, 1996). All chemical
2002). analysis was carried out in triplicate and
the mean value expressed as dry matter.
Supplementing bread with protein con-
centrates and pure amino acids (i.e., ly- Bread making
sine) can be nutritionally effective and Chicken meat powder (CMP) and chicken
shows great promise for improving the diet meat (CM) was added to the commercial
of people who depend on bread as their type white wheat flour and whole wheat
main source of nutrients (Pomeranz and flour blend (containing 70% whole wheat
Shellenberger, 1971). flour, 30% white wheat flour, and 0.4%
Cakmak, H. et al., Chicken meat added bread formulation for protein enrichment,
Food and Feed Research 40 (1), 33-41, 2013
baking improvers). The chicken meat used one day after baking with a texture ana-
in the study was first boiled in water until lyzer, TA XT2i (Stable Microsystems, Sur-
its cold point temperature exceeds 70 oC. rey, United Kingdom), equipped with a
Then skin is removed and meat is debo- cylindrical probe of 36 mm in diameter at a
ned and grounded into small pieces for 3 speed of 1.7 mm,s according to AACC
minutes in a blender (Waring Blender, Dy- method 74 09 (AACC, 1988). Loaf volume
namics, New Hartford, Connecticut, USA). was determined by the rapeseed displa-
The bread formulations were optimized cement method (AACC, 2010). The sen-
using the Detmold method with slight sory evaluations were conducted using
modifications (baking temperature and ranking test with 30 randomly selected
time) (AGF, 1978). For the bread making untrained panellists (Altug and Elmaci,
studies, chicken meat powder and chicken 2005). The ranking test was scaled with
meat at 10, 15, 20, 25, and 30% levels “1” being the lowest score and “6” being
(wet basis) were blended with flour the highest score. Five different sensory
samples. The amount of compressed attributes, crumb colour and crust colour,
yeast (3%) and salt (1.2%) used in these crumb firmness and crust firmness, and
trials were calculated on the flour basis. overall acceptability, were assessed to
After preliminary trials, two types of baking determine the differences among samples
improver were used to improve the using average panel scores. The water ac-
specific volume and the texture of the tivity was measured in duplicate (AG400,
whole wheat breads. All the ingredients Testo, Lenzkirch, Germany).
were mixed with an adequate quantity of The colour was measured in Hunter colour
water, which was determined according to space using a ColorFlex colorimeter
the farinograph water absorption levels (HunterLab, Reston, Virginia, USA) where
and kneaded in a spiral mixer (ISM 10, L is a mea-sure of brightness (0: black,
Inoksan, Bursa, Turkey) to get dough of a 100: white), +a,-a is a measure of red-
moderately stiff consistency. The dough ness, greenness, and +b,-b is yellowness,
was then placed in a fermentation cham- blueness. To reveal the overall effect of
ber (FGM 100, Inoksan, Bursa, Turkey) for enrichment o colour total colour difference
30 min at 30 °C and 75% relative humidity. was calculated according to following for-
After manual aeration of the dough for 1 mula;
minute, the dough was then allowed to
rest in the same fermentation chamber for
30 min and after this period they were
divided into 400 g portions, and moulded Statistical Analysis
in Teflon baking pans (bottom 80 x 80 mm, All data were analyzed with SPSS
top 120 x 120 mm, and a height of software version 16.0 (SPSS, 2007).
100 mm) and proved for 45 min. After the One way analysis of variance was used to
proofing period the breads were baked at compare the mean values and Duncan
220 °C and 25% relative humidity in a pre- post hoc multiple comparison test was
heated oven (FBE010, Inoksan, Bursa, applied with a significance level of p<0.01
Turkey) for 20 min and to avoid dryness of to evaluate the differences between the
the bread crusts, some water being bread samples. The sensory analyses
sprinkled on the pans manually imme- were evaluated using one way analysis of
diately before baking. Loaves were re- variance and Tukey post hoc multiple
moved from pans and subjected to a 4 comparison test with a significance level of
hour cooling period at room temperature p<0.01.
before performing of all analyses.
RESULTS AND DISCUSSION
Bread analysis Evaluation of bread quality
All the breads were sliced into 12.5 mm characteristics
thickness using a bread slicer prior to the The chemical composition and techno-
measurements (Bosch Spot, Stuttgart, logical properties of raw materials and
Germany). Crumb firmness was measured dough are given in Table 1.
Cakmak, H. et al., Chicken meat added bread formulation for protein enrichment,
Food and Feed Research 40 (1), 33-41, 2013
Table 1.
Chemical and technological properties of samples
White wheat Whole wheat Chicken meat Chicken
flour flour powder meat
Moisture (%) 11.94±0.27 9.80±0.31 2.20±0.57 64.67±0.13
a,b a,b a,c a,c
Protein (%) 12.13±0.11 10.39±0.31 78.83±1.77 84.83±3.33
a a a a
Ash (%) 0.62±0.02 1.67±0.03 11.15±1.65 2.89±0.01
Farinograph Results
*
Water absorption (%) 66.1 71.1
*
Development time (min) 1.4 5.5
*
Stability (min) 8.6 1.2
d *
Degree of softening 100 70
e
Extensograph results
d *
Resistance to extension 1313 653
d *
Extensibility 110 79
2 *
Energy (cm ) 158 71
a b c d e th *
Expressed on dry mater basis, Nx5.7, Nx6.25, Brabender unit, 135 minute result, whole wheat flour blend
Table 2.
Quality characteristics of enriched breads
3
Specific volume (dm ,kg) Moisture (%)
Chicken
A-CMP A-CM B-CMP B-CM A-CMP A-CM B-CMP B-CM
(%)*
c e d d e b,c a a
0 2.700 2.700 2.015 2.015 39.835 39.835 36.235 36.235
d d,e c,d c a a,b b a
10 2.965 2.555 1.975 1.705 35.455 39.305 40.260 36.600
c c,d b,c b,c b a c a
15 2.660 2.360 1.905 1.635 36.115 39.095 40.700 38.740
b b,c b,c b,c c a d a
20 2.300 2.445 1.890 1.625 38.520 38.930 41.610 38.310
a b b a,b d c e a
25 1.890 2.090 1.830 1.545 39.440 40.115 42.195 41.215
a a a a f d f a
30 1.800 1.860 1.735 1.425 40.625 41.515 42.815 41.470
∆E crumb ∆E crust
Chicken
A-CMP A-CM B-CMP B-CM A-CMP A-CM B-CMP B-CM
(%)*
0 - - - - - - - -
a a a a e c a a
10 7.000 2.903 1.795 3.103 12.180 4.083 1.868 4.198
b b b b d b d b
15 9.670 3.073 2.030 3.480 11.350 3.318 4.103 4.868
c b c c c a b d
20 10.500 3.108 4.080 4.503 7.580 2.418 3.313 5.530
d c d d a d c b
25 14.350 4.880 4.565 4.703 5.680 13.288 3.755 4.888
e d e e b e e c
30 16.175 8.840 5.130 5.318 5.950 14.800 5.478 5.373
*wet basis
A: white wheat flour bread, B: whole wheat flour blend bread, CMP: chicken meat powder, CM: chicken meat
a-f
values in the same column followed by different superscripts are significantly different (p<0.01).
Cakmak, H. et al., Chicken meat added bread formulation for protein enrichment,
Food and Feed Research 40 (1), 33-41, 2013
The water absorption of white wheat flour meat powder (p<0.01). For whole wheat
and whole wheat flour was found to be breads, firmness was increasing with the
66.1% and 71.1%, respectively by farino- increasing level of enrichment. Those
graph analysis and it is in agreement with samples which have lower specific volume
the observation of Mis et al. (2012), which also have higher crumb firmness in accor-
is also stating that dough extensibility can dance with other studies (Shah et al.,
be predicted based on water absorption 2006; Jaekel et al., 2012)
with its negative effect. For white wheat
The effects of chicken meat powder and
flour, development time was lower and
chicken meat on bread colour are given in
stability was longer than whole wheat flour
Table 2. Total colour difference of bread
blend, these results were also in agre-
crumbs whether they were produced from
ement with the study of Seyer and Gelinas
white flour or not, was significantly diffe-
(2009).
rent from each other (p<0.01). Increasing
The physical and chemical properties of levels of enrichment resulted in higher to-
the bread samples are shown in Table 2. tal colour differences. But the similar effect
In the present study, the moisture content was not clear for the bread crusts. Also the
of the bread samples were between 35.5% crumb images are shown in Figure 1.
and 42.9%. Similarly in another study, the
The effects of chicken meat and chicken
moisture content of bread samples con-
meat powder on water activity are shown
taining different levels of wheat germ and
in Table 2. A significant increase in water
equal ratio of coarse and fine bran addi-
activity was found with an increase in
tions were found between 39.29% and
chicken meat and chicken meat powder
42.25% (Sidhu et al., 1999).
(p<0.01). Similar increasing trend was ob-
Loaf volume is a very important measure served in moisture of enriched breads as
of bread quality (Shah et al., 2006). The expected.
level of enrichment and the type of flour
Typically with those water activity results,
significantly affected the specific volume of
the shelf life of the enriched breads would
enriched breads (p<0.01). Bran has a ne-
be no longer than 4 8 days depending on
gative effect on bread volume (Almeida et
the storage conditions as it is stated by
al., 2013; Ozboy and Koksel, 1999; Seyer
Cauvain and Young (2011), however the
and Gelinas, 2009; Zhang and Moore,
staling kinetics and microbial stability of
1999).
enriched breads during storage should be
White bread enriched with 10% chicken determined in further studies.
meat powder had a higher specific volume
compared to the control sample. The si- Sensory evaluation
milar results were obtained with Welsh Table 3 represents the average scores of
onion powder enriched breads (Seguchi crumb colour, crust colour, crumb firm-
and Abe, 2003) and nut paste enriched ness, crust firmness and overall accepta-
breads (Oliete et al., 2008). bility of enriched breads. Significant diffe-
The addition of chicken meat and chicken rences were obtained for crumb colour,
meat powder increased the protein con- crust colour, and crumb firmness and crust
tents of the white and whole wheat bread firmness with increasing levels of chicken
samples (Table 2). Although the initial pro- meat and chicken meat powder (p<0.01).
tein content of whole bread was higher The overall acceptability of both breads
than the white bread, the highest protein was not perceived as significantly different
content was found for the 30% chicken (p>0.01), except for the whole wheat
meat powder enriched white bread. bread enriched with chicken meat. To re-
Texture analysis results are shown in veal the clear interactions of sensory attri-
Table 2. There is no significant change in butes with enrichment levels, further stu-
the firmness of enriched white bread sam- dies should be implemented in several dif-
ples containing chicken meat and chicken ferent consumer groups.
Cakmak, H. et al., Chicken meat added bread formulation for protein enrichment,
Food and Feed Research 40 (1), 33-41, 2013
Table 3.
Sensory evaluation of enriched breads
Crumb colour Crust colour
Chicken
A-CMP A-CM B-CMP B-CM A-CMP A-CM B-CMP B-CM
(%)*
a a a a a a a a
0 1.00 1.40 2.40 2.50 1.00 1.70 1.45 1.25
b a,b a,b a b a b b
10 2.00 2.25 3.00 2.50 2.15 2.25 2.80 2.55
c b a,b a,b c a b,c c
15 3.35 2.80 3.30 3.20 3.30 2.30 3.90 4.05
d c a,b a,b,c c b b,c c
20 3.75 4.25 3.80 3.50 3.80 4.40 4.05 4.15
e c a,b b,c d b,c c c
25 5.05 5.10 3.85 4.35 5.15 4.85 4.35 4.35
f c b c d c c c
30 5.85 5.20 4.65 4.95 5.60 5.50 4.45 4.65
Crumb firmness Crust firmness
Chicken
A-CMP A-CM B-CMP B-CM A-CMP A-CM B-CMP B-CM
(%)*
a a a a a a a a
0 1.70 2.05 2.90 2.30 1.45 2.85 2.95 2.60
a,b a,b a a,b b a,b a a
10 2.20 2.70 3.30 2.85 3.00 3.05 3.40 3.40
b,c b,c a a,b b a,b a a
15 2.95 3.60 3.30 3.40 3.00 3.10 3.40 3.50
c,d b,c a a,b b,c a,b a a
20 3.90 3.80 3.65 3.65 3.80 3.40 3.55 3.65
d,e c a b c a,b a a
25 4.70 4.35 3.65 4.40 4.70 3.95 3.80 3.85
e c a b c b a a
30 5.55 4.50 4.20 4.40 5.05 4.65 3.90 4.00
Chicken
Overall acceptability
(%)*
0 A-CMP A-CM B-CMP B-CM
a a a a
10 3.00 2.60 2.70 2.15
a a a a,b
15 3.05 3.30 2.80 3.35
a a a a,b
20 3.15 3.45 3.20 3.70
a a a a,b
25 3.80 3.60 3.85 3.75
a a a b
30 4.00 3.70 4.05 4.00
a a a b
4.00 4.35 4.40 4.05
*wet basis
A: white wheat flour bread, B: whole wheat flour blend bread, CMP: chicken meat powder, CM: chicken meat
a-f
values in the same column followed by different superscripts are significantly different (p<0.01)
a)
b)
c)
d)
Figure 1. Crumb images of enriched breads with enrichment levels of 0-10-15-20-25-30% respectively
(a)A-CMP, (b)A-CM, (c)B-CMP, (d)B-CM
Cakmak, H. et al., Chicken meat added bread formulation for protein enrichment,
Food and Feed Research 40 (1), 33-41, 2013
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Cakmak, H. et al., Chicken meat added bread formulation for protein enrichment,
Food and Feed Research 40 (1), 33-41, 2013