BASIC ANALYTICAL CHEMISTRY

CHM 256
LABORATORY CASE STUDY REPORT
(CHROMATOGRAPHY)

TITLE OF CASE STUDY (VIDEO):

SEPARATION OF PIGMENTS FROM THE EXTRACT OF SPINACH LEAVES

BY PAPER CHROMATOGRAPHY

NO NAME OF MEMBERS STUDENT ID

GROUP :

LECTURER’S NAME :

DATE OF SUBMISSION :

1
4.0 RESULTS & DISCUSSION

Figure 1: How to find R f in Paper Chromatography

A
Rf=
B

Distance travelled by the compound

Rf=
Distance travelled by the solvent .

Colour Rf Pigment
Yellow-orange 6.67 Carotene
=0.95
7
Yellow 5.25 Xanthophyll
=0.71
7
Dark Green 4.55 Chlorophyll a
=0.65
7
Yellowish Green 3.15 Chlorophyll b
=0.45
7

Table 1: Identify R f value and the Pigment

Figure 3: Pigment in Paper Chromatography