Professional Documents
Culture Documents
.....................................................................................................................................................................................
.....................................................................................................................................................................................
.....................................................................................................................................................................................
.....................................................................................................................................................................................
.....................................................................................................................................................................................
.....................................................................................................................................................................................
…………………………………………………………………………………………………………………………………………………………………….……[3]
2. Over a period of ten years, an antibiotic was used in a hospital to treat an infection. Fig. 5.1 shows the
amount of antibiotic used and the proportion of bacteria that survived treatment with the antibiotic over this
period of time.
Fig. 5.1
.................................................................... [1]
Page -1
(b) State the period of time during which the antibiotic was most effective at treating the infection in the
hospital.
.................................................................... [1]
(c) Suggest and explain possible causes for the increase in the proportion of bacteria that survived treatment
with the antibiotic after 1994.
.....................................................................................................................................................................................
.....................................................................................................................................................................................
.....................................................................................................................................................................................
.....................................................................................................................................................................................
.....................................................................................................................................................................................
.....................................................................................................................................................................................
.....................................................................................................................................................................................
.....................................................................................................................................................................................
.....................................................................................................................................................................................
................................................................................................................................................................................ [5]
3. Microorganisms can be grown in the laboratory on agar (a jelly-like medium) in a Petri dish.
(a) Name a nutrient that should be added to ensure the maximum rates of
A student is provided with a Petri dish containing agar with the optimum concentration of nutrients. Explain how
the student could speed up the rate of growth of microorganisms growing in this Petri dish.
.....................................................................................................................................................................................
.....................................................................................................................................................................................
.....................................................................................................................................................................................
.................................................................................................................................................................................[2]
Page -2
Fig. 4.1 shows the stages in an experiment to show the effect of different antibiotics on microorganisms.
The microorganisms originally came from the throat of a person suffering from a throat infection.
(i) identify by letter the antibiotic which might be best for treating the infection,
...................................... [1]
(ii) state which type of microorganism was probably causing the throat infection and give a reason for your
answer.
reason .....................................................................................................................................................................[2]
(d) Antibiotic F used to be the most effective in treating the infection. Suggest and explain why it has become
less effective.
.....................................................................................................................................................................................
.....................................................................................................................................................................................
.....................................................................................................................................................................................
.....................................................................................................................................................................................
.....................................................................................................................................................................................
.....................................................................................................................................................................................
.................................................................................................................................................................................[3]
Page -3
4. Fig. 4.1 shows the change in percentage of disease-causing bacteria that were resistant to the antibiotic
penicillin from 1991 to 1995.
(a) (i) Describe the change in the percentage of bacteria resistant to penicillin between 1991 and 1995.
.....................................................................................................................................................................................
.....................................................................................................................................................................................
.....................................................................................................................................................................................
…………………………………………………………………………………………………………………………………………………………………..……..[2]
.....................................................................................................................................................................................
.....................................................................................................................................................................................
.....................................................................................................................................................................................
.....................................................................................................................................................................................
.....................................................................................................................................................................................
.....................................................................................................................................................................................
.....................................................................................................................................................................................
…………………………………………………………………………………………………………………………………………………………………………..[4]
Page -4