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TYPES OF BASILLUS:
Some types of Bacillus bacteria are harmful to humans, plants, or other organisms.
For example, B. cereus sometimes causes spoilage in canned foods and food poisoning of short
duration. B. subtilis is a common contaminant of laboratory cultures and is often found on
human skin. Most strains of Bacillus are not pathogenic for humans but May, as soil organisms,
infects humans incidentally. A notable exception is B. anthracis, which causes anthrax in humans
and domestic animals. B. thuringiensis produces a toxin (Bt toxin) that causes disease in insects.
BACILLUS CEREUS:
Bacillus cereus (B. cereus) is classified as a gram-positive, aerobic or facultative anaerobic,
spore former, motile, pathogenic, and opportunistic bacterium capable of producing resistant
endospores in the presence of oxygen. B. cereus is widely distributed in the environment, namely
soil, where spores persist under adverse conditions. B. cereus can grow over a wide temperature
range (8–55 °C), but it is not well suited to tolerate low pH values (minimum 5–6) or water
content (minimum water activity 0.95).
BACILLUS SUBTILIS:
Bacillus subtilis is a rod-shaped, Gram-positive bacterium that forms spores. Efficient systems of
genetic analysis have helped B. subtilis to become a paradigm for the study of spore formation
and of low GC Gram-positive bacteria. B. subtilis can grow in minimal media containing only
essential salts and carbon, nitrogen, and phosphorus sources. It uses a range of regulatory
mechanisms to control the expression of genes for both biosynthetic and degradative pathways.
Growth and division require the synthesis and remodeling of the thick wall that surrounds the
bacterial cell.
BACILLUS ANTHRACIS:
Bacillus anthracis is a large (1–1.5×3–10 μm) gram-positive sporulating rod, with square or
concave ends. It can readily grow on sheep blood agar leading to the formation of rough gray
white colonies of 4 to 5 mm, with characteristic comma-shaped or “comet-tail” protrusions . The
virulence of B. anthracis largely depends on its two main toxins, lethal toxin and edema toxin.
BACILLUS THURINGIENSIS:
Bt is a Gram-positive, spore-forming, facultative bacterium, about 0.5–1.0×2–5 µm in size.
There are over 100 subspecies and varieties divided into 70 serotypes, based on flagellar H
antigens, host specificity, and presence of plasmids.
In nature, BT has been reported from all continents. It has been isolated from diseased insects,
from the soil, from granary dust, and from the leaf surface of many plants. This bacterium is
easily grown, sporulates well under laboratory conditions, and uses a variety of carbohydrates as
carbon and energy sources. BT cells can grow within a range of 10–45°C at an optimum pH of
5.7 and can tolerate 7% NaCl. The most convenient method of isolation is “acetate selection,”
MEDICALLY AGENT:
Medically useful antibiotics are produced by B. subtilis (bacitracin). In addition, strains of B.
amyloliquefaciens bacteria, which occur in association with certain plants, are known to
synthesize several different antibiotic substances, including bacillaene, macrolactin, and
difficidin. These substances serve to protect the host plant from infection by fungi or other
bacteria and have been studied for their usefulness as biological pest-control agents.
ENZYME ENCODING:
A gene encoding an enzyme known as barnase in B. amyloliquefaciens is of interest in the
development of genetically modified (GM) plants. Barnase combined with another protein
synthesized by B. amyloliquefaciens known as barstar, forming the barnase-barstar gene system,
was used to develop a line of non-self-fertilizing transgenic mustard (Brassica juncea) plants
with enhanced outbreeding capability. The gene controlling production of the Bt toxin in B.
thuringiensis has been used in the development of GM crops such as Bt cotton.
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Research aricle:
Bacillus As Potential Probiotics: Status, Concerns, and
Future Perspectives:
Summarize :
Spore-forming bacilli are being explored for the production and preservation of food for many
centuries. The inherent ability of production of large number of secretory proteins, enzymes,
antimicrobial compounds, vitamins, and carotenoids specifies the importance of bacilli in food
chain. Additionally, Bacillus spp. are gaining interest in human health related functional food
research coupled with their enhanced tolerance and survivability under hostile environment of
gastrointestinal tract. Besides, bacilli are more stable during processing and storage of food and
pharmaceutical preparations, making them more suitable candidate for health promoting
formulations.
Further, Bacillus strains also possess biotherapeutic potential which is connected with their
ability to interact with the internal milieu of the host by producing variety of antimicrobial
peptides and small extracellular effector molecules. Nonetheless, with proposed scientific
evidences, commercial probiotic supplements, and functional foods comprising of Bacillus spp.
had not gained much credential in general population, since the debate over probiotic vs
pathogen tag of Bacillus in the research and production terrains is confusing consumers. Hence,
it’s important to clearly understand the phenotypic and genotypic characteristics of selective
beneficial Bacillus spp.
The interest in the field of beneficial microbes has emerged multiple folds since its inception by
the Russian Noble laureate, Elie Metchnikoff. The term Probiotics, taken as an un-challenged
synonym to beneficial microbes, has gained popularity over the years and has found application
in several general health and clinical scenarios. Probiotics are live microorganisms, which when
administered in adequate amounts confer health benefits to the host.
Regulatory/Legislative Status:
The “health claims” of commercially available bio-therapeutics are allied to the viability, activity
and composition of component microorganisms. Likewise, the entitlements of probiotic products
being marketed as functional food, dietary supplement or drug are also elucidated by the state of
probiotic strains. Thereby, the quality of commercial probiotic products is an important issue to
be considered for regulation. Several researchers have identified discrepancies between labeled
and actual contents of commercial probiotic products.
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